• Journal of the Korean Applied Science and Technology
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• v.36 no.2
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• pp.434-447
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• 2019

Polyethylene glycol (PEG) is widely used in cosmetics as a surfactant, detergent and emulsifier. During the manufacturing process, 1,4-dioxane, which is toxic to humans, can be produced as a by-product by dimerization of ethylene oxide. As consumers' interest in cosmetic ingredients has increased, the need for safe emulsion research without PEG ingredients in the personal care market has increased. With increasing consumer interest in cosmetic ingredients, the need for safer emulsion research without the PEG ingredient in the personal care market has increased. In this study, we aimed to develop and stabilize nanoemulsion formulation without PEG. Response Surface Methodology (RSM) was used to develop optimized nanoemulsion formulations. Surfactant content (2~4%), oil content (4~8%) and polyol content (12~24%) were set as independent variables as a result of preliminary experiments for determining independent variables and ranges. The particle size, zeta potential, turbidity, and polydispersity index of the formulation were measured as response variables. As a result of measurement of the prepared nanoemulsion by FIB (Focused ion beam), spherical particles were found to have a size distribution of 100 to 200 nm. The stability of each formulation was evaluated for 30 days at each temperature ($4^{\circ}C$, $25^{\circ}C$, and $45^{\circ}C$). The optimal formulation considering the optimum particle size, turbidity, polydispersity index and zeta potential was found to be surfactant (2%), oil (8%) and polyol (24%).

• The Journal of the Convergence on Culture Technology
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• v.5 no.3
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• pp.317-326
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• 2019

The present study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): group I-normal group, group II-DSS control group, group III-DSS + sulfasalazine (30 mg/kg), group IV-DSS + sulfasalazine (60 mg/kg), group V-DSS + sulfasalazine (30 mg/kg) + Radish Extract mixture (30 mg /kg) (SRE). DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling molecules through suppression both MAPK) and nuclear factor-kappa B (NF-${\kappa}B$) signaling pathways, and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of anti-oxidant enzyme including SOD and Catalase. This is the first report that Radish extract mixture combined with sulfasalazine protects against experimental UC via the inhibition of both inflammation and apoptosis, very similar to the standard-of-care sulfasalazin.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.12
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• pp.1873-1879
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• 2010

This study was conducted to estimate the heavy metal contents of vegetables available on the markets in Seoul area. Concentrations of mercury (Hg), lead (Pb), cadmium (Cd), arsenic (As), chrome (Cr), nickel (Ni), copper (Cu), and zinc (Zn) were measured in 300 samples using a mercury analyzer and inductively coupled plasma optical emission spectrometer (ICP-OES) after wet digestion. The average values of heavy metals in vegetables were as follows [mean (minimum~maximum), mg/kg]; Hg: 0.0005 (N.D~0.007), Pb: 0.011 (N.D~0.259), Cd: 0.012 (N.D~0.188), As: 0.002 (N.D~0.142), Cr: 0.100 (0.019~0.954), Ni: 0.093 (0.003~1.231), Cu: 1.098 (0.072~36.29), and Zn: 3.48 (0.485~21.31). The heavy metal contents of vegetables available on the markets in Seoul were almost the same as or lower than those reported in other studies. The weekly average intakes of mercury, lead and cadmium from vegetables take 0.44~7.71% of PTWI (Provisional Tolerable Weekly Intakes) that the FAO/WHO Joint Food Additive and Contaminants Committee sets for evaluation of food safety.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.230-234
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• 2011

According to the Codex committee, the maximum allowable level for lead in fruits is 0.1 mg/kg. This survey was conducted as a surveillance program following the establishment of safety guideline for fruits in Korea. Concentrations of lead (Pb), cadmium (Cd), arsenic (As) and mercury (Hg) were measured in 927 samples using a ICP-MS and a mercury analyzer. The recoveries of microwave digestion method were 86.0-110.4% for Pb, 81.0-104.0% for Cd and 82.0-104.7% for As by standard addition method. The recovery of direct mercury analyzer was 106.5% for Hg. The average levels of Pb in ${\mu}g/kg$ were $10.0{\pm}12.8$ for apple, $8.8{\pm}10.9$ for pear, $4.1{\pm}4.4$ for persimmons, $14.9{\pm}12.3$ for mandarin, $7.1{\pm}6.5$ for orange, $3.1{\pm}3.3$ for banana, $8.8{\pm}8.9$ for kiwi, and $9.3{\pm}9.7$ for mango. The average levels of Cd in ${\mu}g/kg$ were $0.4{\pm}0.3$ for apple, $2.0{\pm}1.6$ for pear, $0.3{\pm}0.3$ for persimmon, $0.1{\pm}0.1$ for mandarin, $0.1{\pm}0.1$ for orange, $1.3{\pm}1.8$ for banana, $0.5{\pm}0.5$ for kiwi, and $0.7{\pm}0.6$ for mango. The average levels of As in ${\mu}g/kg$ were $2.0{\pm}2.1$ for apple, $1.2{\pm}1.3$ for pear, $1.5{\pm}1.2$ for persimmon, $0.8{\pm}0.3$ for mandarin, $1.5{\pm}0.5$ for orange, $1.8{\pm}1.2$ for banana, $1.6{\pm}1.5$ for kiwi, and $1.2{\pm}1.5$ for mango. The average levels of Hg in ${\mu}g/kg$ were $0.5{\pm}0.4$ for apple, $0.3{\pm}0.2$ for pear, $0.2{\pm}0.1$ for persimmon, $0.2{\pm}0.1$ for mandarin, $0.2{\pm}0.1$ for orange, $0.2{\pm}0.0$ for banana, $0.2{\pm}0.2$ for kiwi, and $0.6{\pm}0.2$ for mango. Based on the Korean public nutrition report 2005, these levels (or amounts) are calculated only at 0.17% for Pb, 0.013% for Cd and 0.006% for Hg of those presented in provisional tolerable weekly Intake (PTWI) which has been established by FAO/WHO. Therefore, the levels presented here are presumed to be adequately safe.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.9
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• pp.1464-1470
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• 2005

To assure the safety of the major edible seaweeds, we collected the 176 samples of seaweeds such as laver(Porphyra sp.), sea mustard (Undaria pinnatifida), sea tangle (Laminaria japonica) and sea lettuce (Enter-omorph sp.) from Korean coast. We investigated the contents of the trace metals in the edible seaweeds, and the values were expressed as the dry weight of sea weeds. The mean levels of the trace metals were high in the oder of Zn ($36.19{\pm}27.14 \;{\mu}g/g$), Mn ($28.75{\pm}28.03\;{\mu}g/g$), and Cu ($6.82{\pm}7.62\;{\mu}g/g$), which are necessary metals in the human body, and then followed by Cr ($1.54{\pm}2.07\;{\mu}g/g$), Ni ($1.29{\pm}2.57\;{\mu}g/g$), Pb ($0.89{\pm}1.11\;{\mu}g/g$), Cd ($0.72{\pm}0.80\;{\mu}g/g$) and Hg ($0.01{\pm}0.02\;{\mu}g/g$). The contents of Cd and Zn were high in the laver, Cr, Ni and Pb were detected highly in sea lettuce, and Hg was detected highly in sea tangle. Significant linear correlations were obtained among 28 different pairs of metals, some of them highly correlated (r>0.5, p<0.05),such as Ni-Mn, Zn-Ni, Cu-Pb and Cu-Zn in laver, Ni-Zn in sea mustard, Cu-Pb, Cu-Zn and Mn-Pb in sea tangle, and Ni-Cr in sea lettuce. And, the trace metal contents of seaweeds varied with parts and kinds of samples. The average weekly intakes of Hg, Cd and Pb from seaweeds were about $0.07{\~}3.57\%$ of PTWI (Provisional Tolerable Weekly Intakes) which has set by FAO/WHO to evaluate the safeties of trace metals in food. Therefore, it was found that they showed very safe levels in the edible seaweeds.

• Journal of Food Hygiene and Safety
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• v.39 no.3
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• pp.250-259
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• 2024

In this study, we investigated in pesticide residues and heavy metals in fermented liquor products (wine, beer, makgeolli). Targeted analysis of 400 pesticide residues in the sample was performed using the quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method, followed by gas chromatography-tandem mass spectrometry (MS/MS) and LC-MS/MS. The contents of heavy metals (Pb, Cd) were determined by ICP-MS using the microwave method. The mercury was measured using a mercury analyzer. From the analysis of 150 cases, 102 (68.0%) cases of fermented liquor were detected, and 35 pesticide residues (including metalaxyl, mandipropamid, azoxystrobin, and fenhexamid) were detected among the 400 pesticide residues tested. Pb, Cd, and Hg were tested in 150 samples. Lead was detected in 73 samples (48.7%), cadmium in 9 samples (6.0%), and mercury in 36 samples (24.0%). Exposure assessment was conducted to determine the safety of the detected pesticide residues and heavy metals. According to this assessment, the pesticide residues and heavy metals showed very low %ADI values (less than 1%).

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.11
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• pp.1278-1285
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• 2017

Accumulation of excess low density lipoprotein (LDL) cholesterol in the blood can initiate and accelerate atherosclerosis. Statins mediate the transactivation of proprotein convertase subtilisin/kexin type 9 (PCSK9), which in turn limits their cholesterol-lowering effects via LDL receptor (LDLR) degradation. The objective of this study was to investigate whether or not Allium tuberosum (AT) regulates LDLR and PCSK9. Mice were fed a low fat control diet (LD) or Western diet (WD) supplemented with AT (1%, w/w). AT significantly attenuated total and LDL cholesterol levels in mice fed WD (P<0.05). AT also significantly inhibited hepatic PCSK9 gene expression (P<0.05) while AT maintained hepatic LDLR gene expression. To further investigate AT-mediated PCSK9 regulation, HepG2 cells were treated with 10% delipidated serum (DLPS) in the presence or absence of AT. Non-toxic level of AT dose-dependently increased the LDLR protein level, and AT at $400{\mu}g/mL$ markedly inhibited PCSK9 protein expression. Similarly, AT significantly increased LDLR gene expression, whereas it significantly down-regulated PCSK9 gene expression. AT-mediated reduction of PCSK9 gene expression is likely due to decreased hepatic nuclear factor $1{\alpha}$ ($HNF1{\alpha}$) expression, but not SREBP2 in HepG2 cells under lipid-depleted conditions. AT-mediated PCSK9 inhibition contributed to LDLR protein stabilization via protection against LDLR lysosomal degradation in HepG2 cells under lipid-depleted conditions. Further investigation is warranted to determine the active components of AT and whether or not these components are effective in reducing hypercholesterolemia.

• Korean Journal of Food Science and Technology
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• v.39 no.2
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• pp.222-227
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• 2007

The effects of chlorine dioxide ($ClO_2$) treatment on the quality changes of pork and beef were examined. Pork belly and beef tenderloin samples were treated with 30, 50, and 100 ppm of $ClO_2$ solution, respectively, and stored at $4{\pm}1^{\circ}C$. The $ClO_2$ treatment of pork and beef during storage decreased total aerobic bacteria, yeast, and mold counts with increasing concentration of $ClO_2$. The total aerobic bacterial counts for the pork belly treated at 100 ppm of $ClO_2$ increased from 1.48 log CFU/g immediately following treatment to 4.73 log CFU/g after 10 days, while the control increased from 2.19 log CFU/g to 6.22 log CFU/g. For the beef tenderloin, the total aerobic bacterial counts increased from 3.98 log CFU/g to 5.97 log CFU/g after 10 days, and a $ClO_2$ treatment at 100 ppm resulted in an increase from 3.13 log CFU/g to 4.73 log CFU/g. The pH and volatile basic nitrogen (VBN) values of the $ClO_2-treated$ pork and beef, as well as the control groups, increased during storage, and there were no significant changes among the treatments. The thiobarbituric acid reactive substance (TBARS) values of the $ClO_2-treated$ samples were slightly higher than those of the control. Sensory evaluation results showed that the pork and beef samples were not acceptable at day 8 and 6 of storage, respectively. These results indicate that $ClO_2$ treatment could be useful in improving microbial safety and quality of both pork and beef.

• Journal of Food Hygiene and Safety
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• v.27 no.2
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• pp.127-132
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• 2012

This study was conducted to investigate of changes for organic acids in takju during storage conditions. Two types of takju, unsterilized and sterilized, were used. Acetic acid, citric acid, lactic acid, malic acid and succinic acid were detected in sample. Residues of malic acid was not detected after 7 days storage in room temperature($25^{\circ}C$). In contrast to lactic acid was increased by times after storage. Acetic acid concentrations was gradually increased in room temperature but was not changed in cold temperature($4^{\circ}C$). After storage for 10 days, the organic acid residues lactic acid > citric acid > succinic acid > malic acid > acetic acid in unsterilized takju. Compared to unsterilized sample, organic acid residues in sterilized sample was not significantly changed. Consequently, organic acids residues mainly affected by sterilization.

• Journal of Food Hygiene and Safety
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• v.27 no.1
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• pp.81-86
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• 2012

The present study was evaluated the antibacterial effect of the combination of $Coptidis$ $rhizoma$, $Glycyrrhiza$ $uralensis$ Fischet, $Schizandra$ $chinensis$ and $Corni$ $Fructus$(1:1:1) extracts(CGSC10). Furthermore, the effectiveness of CGSC10, sodium chlorate, and the combination of CGSC10 and sodium chlorate(CGSCS10) against $E.$ $coli$ O157:H7 infection was studied using ICR female mice. During the incubation period, the dose of 5, 10, and 20% CGSC10 was inhibited the growth of $E.$ $coli$ O157:H7 by 34.7, 60.2, and 76.4%, respectively. For 7 days after single challenge with $E.$ $coli$ O157:H7, forty female ICR mice were divided into four experimental groups which were administered in drinking water with saline, 10% CGSC10, 15 mM sodium chlorate, and CGSCS10, respectively. On the 3rd day, the number of $E.$ $coli$ O157:H7 in mouse feces was significantly decreased by administration of CGSC10, 15 mM sodium chlorate, and CGSCS10 ($p$ < 0.001). On the 7th day-after administration, CGSC10, sodium chlorate, and CGSCS10 were decreased the number of $E.$ $coli$ O157:H7 by 27.1, 67.7, and 83.3%, respectively. According to the results of the present study, administration of CGSCS10 to mice can reduce the severity of $E.$ $coli$ O157:H7 infection. In addition, it is suggested that CGSCS10 represents a good candidate for the treatment of enteric infections in domestic animals.