• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.5
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• pp.848-853
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• 2001

This research was conducted to study the effects on antigenicities (allergenicity) and structural changes of gamma-irradiated hen’s egg albumin (ovalbumin, OVA) by heating. Three groups of OVA solution (2.0 mg/mL) were prepared; 1) heat treatment; 2) irradiation after heating; 3) heating after irradiation. Samples were isothermally heated and/or irradiated at the absorption dose of 10 kGy. Competitive indirect ELISA was individually formatted with egg-allergic patients IgE (P-IgE), and mouse murine monoclonal IgG (M-IgG) and rabbit polyclonal IgG (R-IgG) for evaluating bindinhg abilities of antibodies to OVA in the sample solutions. Binding abilities of antibodies to thermally denatured OVA were changed : R-IgG to the sample treated with above 6$0^{\circ}C$, M-IgG to that above 7$0^{\circ}C$, and P-IgE to that above 8$0^{\circ}C$, respectively. P-IgE did not well recognize OVA heated at 8$0^{\circ}C$ and the above. However, binding abilities of M-IgG and R-IgG highly in creased. Significant differences of binding abilities were not observed in all samples with the combination of heat treatment and irradiation, regardless the order of the treatment. Turbidity of samples in creased both by heating and by irradiation, and the increase by irradiation was much higher than by heating. These results showed that allergenicity of OVA reduced by gamma irradiation was not affected by heating.

• Food Science and Preservation
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• v.30 no.2
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• pp.300-310
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• 2023

This study was carried out to investigate the effect of the extraction conditions for persimmon (Diospyros kaki Thunb.) leaf tea (PLT) on its color quality and antioxidant properties. The amount of persimmon leaf (PL) powder and pH influenced the PLT's color and antioxidant capacity. As the amount of PL powder in tea increased, lightness decreased while yellowness increased. The PLT with the highest amount of PL (10 mg/mL) exhibited the highest 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity and ferric reducing antioxidant power (FRAP). In addition, the PLT with the highest PL showed the highest total polyphenol and flavonoid contents. Subsequently, PLT was prepared using 10 mg/mL PL powder under varying pH conditions. As pH increased from 4 to 7, lightness decreased while redness and yellowness increased. Antioxidant capacity was determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and FRAP assay showed that lower pH exhibited higher antioxidant capacity. The PLT extracted under the lowest pH of 4 showed higher polyphenol and flavonoid contents than that extracted under higher pH conditions. Overall, PLT extraction using a solvent with pH 4.0 showed better antioxidant activities and higher amounts of polyphenolic compounds. Simultaneously, lesser lightness, redness, and yellowness were detected in PLT extracted under pH 4 conditions. In conclusion, to acquire a better functional health benefit in terms of antioxidant capacity, preparing PLT under pH 4 conditions is suggested.

• Korean Journal of Poultry Science
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• v.42 no.2
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• pp.109-116
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• 2015

Ogye rooster sperm chromatin status can be detected using well established sperm assays. In this paper, a simple and fast method to monitor rooster sperm chromatin status could be employed in field for assessment of chicken sperm quality. Using standard bright field microscope, Diff-Quik stains can be reproducibly, easily and routinely monitored with simple staining. The presence of abnormal chromatin staining of rooster sperm was determined by darker stain in head. In the fresh semen, the viabilities of three tested Ogye spermatozoa were 93.53%, 82.42% and 90.63% and normal chromatin rates were 87.96%, 74.25% and 85.10% respectively. However, after freezing, the rates of viability of thawed semen were reduced to 69.58%, 61.98% and 72.20% and normal chromatin rate also reduced to 58.91%, 48.49% and 63.34%. A significant correlation between live sperm and normal sperm nuclei was 0.875 in fresh semen and 0.513 in frozen semen. After incubation of sperm at $37^{\circ}C$ for 5min, the rates of viability, chromatin normality and sperm head activity were shown as $90.63{\pm}1.28%$, $82.44{\pm}8.09%$ and $66.68{\pm}10.29%$ in fresh semen. However, the rates of thawed semen were reduced to $67.92{\pm}7.55%$, $56.92{\pm}12.15%$ and 47.32{\pm}5.02%, respectively. The relationship between chromatin normality and sperm head movements in fresh and thawed semen were 0.564 and 0.540, respectively. With these results, the chicken sperm normality could be assessed by the Diff-Quik staining that could be used for chromatin status of sperm head and activated morphology of live spermatozoa, as a simple and rapid staining method.

• Nuclear Medicine and Molecular Imaging
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• v.41 no.4
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• pp.299-308
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• 2007

Purpose: The aim of this study was to develop a bioinformatics software and to test it in serum samples of papillary thyroid cancer using mass spectrometry (SELDI-TOF-MS). Materials and Methods: Development of 'Protein analysis' software performing decision tree analysis was done by customizing C4.5. Sixty-one serum samples from 27 papillary thyroid cancer, 17 autoimmune thyroiditis, 17 controls were applied to 2 types of protein chips, CM10 (weak cation exchange) and IMAC3 (metal binding - Cu). Mass spectrometry was performed to reveal the protein expression profiles. Decision trees were generated using 'Protein analysis' software, and automatically detected biomarker candidates. Validation analysis was performed for CM10 chip by random sampling. Results: Decision tree software, which can perform training and validation from profiling data, was developed. For CM10 and IMAC3 chips, 23 of 113 and 8 of 41 protein peaks were significantly different among 3 groups (p<0.05), respectively. Decision tree correctly classified 3 groups with an error rate of 3.3% for CM10 and 2.0% for IMAC3, and 4 and 7 biomarker candidates were detected respectively. In 2 group comparisons, all cancer samples were correctly discriminated from non-cancer samples (error rate = 0%) for CM10 by single node and for IMAC3 by multiple nodes. Validation results from 5 test sets revealed SELDI-TOF-MS and decision tree correctly differentiated cancers from non-cancers (54/55, 98%), while predictability was moderate in 3 group classification (36/55, 65%). Conclusion: Our in-house software was able to successfully build decision trees and detect biomarker candidates, therefore it could be useful for biomarker discovery and clinical follow up of papillary thyroid cancer.

• Journal of Food Hygiene and Safety
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• v.20 no.4
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• pp.232-236
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• 2005

This study was performed to find out the patterns of Listeria spp. contamination during Kimbab preparation at the specialized restaurants. Samples were collected from ingredients, containers, equipments, and environments from two Kimbab restaurants from July through September, 2004. Isolation rate of Listeria spp. was $43.2\%$ among 264 samples. Strains isolated were Listeria innocua $35.9\%$, L. murrayi $6.8\%$, and L. grayii $0.3\%$. No L. monocytogenes was detected. Contamination level of ingredients, containers, equipments, and Kimbab sampled during afternoon was all significantly higher than the samples collected during morning(P < 0.05). The most influencing factor of contamination was improper handling or no disinfection procedures during Kimbab preparation. Safety controls are recommended as follows; prevention of cross-contamination during ingredients Preparation, temperature control for ingredients and Kimbab during holding, cleaning and disinfection of chopping boards and knives during preparation processing, and frequent changes of disposable gloves. Kimbab restaurants should recognize the importance of safety control using the Good Hygienic Practices.

• Bulletin of the Korean Space Science Society
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• 2003.10a
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• pp.62-62
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• 2003

과학위성 1호에는 위성의 임무를 수행하기 위하여 광학계, 구조부, 및 전자부 등 여러가지 부품들이 실장되는데, 그 중 전자부의 가장 중요한 부품 중의 하나인 인쇄회로기판(Printed Circuit Board, PCB)의 우주환경에서의 특성 대해서 논의하고자 한다. Solder Resistor(Solder Mask)의 화학성분이 위성체가 작동하는 우주환경에서 위성체 임무수행 시 발생할 수 있는 out-gassing으로 인해 위성체가 본연의 임무 실패라는 결과를 초래할 수 있다 NASA 및 ESA의 Out-gassing에 관한 규정과 TRW에 의한 KOMSAT에 사용된 재료의 진공상태의 Outgassing에 관한 내용에 의하면, 재료의 진공상태와 Out-gassing은 America Society for Testing and Materials에서 제시한 ASTM E959 기준에 따라 제작된다. 일반적으로 우주 환경에서 광학계나 전자부의 원활한 동작을 위해서는 인쇄 회로 기판의 총 질량손실(Total Mass Loss, TML)은 1.00%을 넘지 말아야 하며, 휘발성 응축 질량 (Collected Volatile Condensable Mass, CVCM)은 0.1% 미만이어야 한다. Total Mass Loss(TML) 방법은 대기중에서 측정한 질량과 진공 조건에서 변화되는 질량을 측정함으로써 진공조건에서의 탈기체 특성을 측정하는 방법이다. 본 연구에서는 Solder Resistor(Solder Mask)의 탈기체 측정을 위한 진공챔버의 측정방법 및 진공 형성 과정을 기술하고 실제 과학위성1호에 장착될 시료를 예로 들어 인쇄회로기판에 입힌 Solder Resistor(Solder Mask)가 우주환경인 진공상태에서 위성체 부품의 작동 시 발생할 수 있는 탈기체되는 정도를 질량의 변화분으로 측정하여 위성체가 우주 환경에서 본연의 임무를 안전하게 수행할 있는지를 검증하였다.부분이다.다.향을 해석하고 시뮬레이션 하였다.Device Controller)는 ECU로부터 명령어를 받아서 arm 및 safe 상태에 대한 텔리 메트리 데이터를 제공한다 그리고, SAR(Solar Array Regulator)는 ECU로부터 Bypass Relay 및 ARM Relay에 관한 명령어를 받아 수행되며 그에 따른 텔리 메트리 데이터를 제공한다. 마지막으로 EPS 소프트웨어를 검증하는 EPS Software Verification을 수행하였다 전력계 소프트웨어의 설계의 검증 부분은 현재 설계 제작된 전력계 .소프트웨어의 동작 특성 이 위성 의 전체 운용개념과 연계하여 전력계 소프트웨어가 전력계 및 위성체의 요구조건을 만족시키는지를 확인하는데 있다. 전력계 운용 소프트웨어는 배터리의 충ㆍ방전을 효율적으로 관리해 3년의 임무 기간동안 위성체에 전력을 공급할 수 있도록 설계되어 있다this hot-core has a mass of 10sR1 which i:s about an order of magnitude larger those obtained by previous studies.previous studies.업순서들의 상관관계를 고려하여 보다 개선된 해를 구하기 위한 연구가 요구된다. 또한, 준비작업비용을 발생시키는 작업장의 작업순서결정에 대해서도 연구를 행하여, 보완작업비용과 준비비용을 고려한 GMMAL 작업순서문제를 해결하기 위한 연구가 수행되어야 할 것이다.로 이루어 져야 할 것이다.태를 보다 효율적으로 증진시킬 수 있는 대안이 마련되어져야 한다고 사료된다.$\ulcorner$순응$\lrcorner$의 범위를 벗어나지 않는다. 그렇기 때문에도 $\ulcorner$순응$\lrcorner$과 $\ulcorner$표현$\lrcorner$의 성격과 형태를 외형상으로

• Journal of the Korean Chemical Society
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• v.53 no.6
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• pp.765-773
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• 2009

The purpose of this study is to identify secondary chemistry teachers' abilities to carry-out experiment lesson successfully. As far as the research method is concerned, literature reviews, analyses of abilities to carry-out experimentation from science textbooks, and a survey of science teachers' perceptions of experimental ability were employed along with the progressive discussions among the authors. In order to identify secondary chemistry teachers' abilities to carry-out experimentation, we divided the experiment lesson into three stages of preparation, conduct and arrangement, and management of lab, and added the stage of safety. Each stage is classified into sub-areas, and the sub-area consists of subordinate elements. The safety stage was included separately to emphasize the importance of the safety issue in lab and experimental activities. The secondary chemistry teachers' abilities to carry-out experimentation are the abilities to perform experiments in person, presupposing the instruction of experiment, and can be featured with the use of reagent and measuring instruments, the preparation of sample, designing experiment, correct experimental habit and skillfulness, data processing, analysis and reasoning, and management of lab and safety.

• Korean Journal of Mineralogy and Petrology
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• v.35 no.4
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• pp.431-445
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• 2022

A quantitative phase analysis method of X-ray powder diffraction was studied to determine the mineral content of clay-rich rocks practically as well as effectively. For quantitative X-ray powder diffraction analysis of the clay-rich rocks, it is necessary to prepare whole-rock powder samples with a random orientation by side mounting method. In addition, for the identification of the clay minerals in the rock, it is required to prepare an oriented mount specimen with a clay particle size of 2 ㎛ or less, ethylene glycol treatment, and heat treatment. RIR (reference intensity ratio) and Rietveld method were used for the quantitative analysis of the clay-rich rocks. It was possible to obtain the total clay and the non-clay minerals contents from the whole-rock X-ray diffraction profiles using the RIR values. In addition, it was possible to calculate the relative content of each clay mineral from the oriented X-ray diffraction profiles of the clay particle size and assign it to the total clay. In the Rietveld method of whole-rock X-ray diffraction, effective quantitative values were obtained from the Rietveld diffraction patterns excluded the region of less than 10 degrees (2θ). Similar quantitative values were shown in not only the RIR but the Rietveld methods. Therefore, the analysis results indicate a possibility of a routine quantitative analysis of clay-rich rocks in the laboratory. However, quantitative analysis of clay minerals is still a challenge because there are numerous varieties of clay minerals with different chemical and structural characteristics.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2004.06a
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• pp.223-224
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• 2004

고준위폐기물 혹은 사용후 핵연료의 처분용기 재질은 각 국의 처분개념과 처분공의 주위 환경에 따라 달라질 수 있다. 용기의 후보재질로는 탄소강, 스텐레스 강, 구리, 니켈, 티탄 혹은 이들의 합금이 주로 고려되고 있으나, 국내에서는 아직 선정되지 않았다. 국내 처분환경에서 이들 재질의 부식특성을 조사하고자 모의 화강암 지하수를 가해 만든 겔 상태의 경주 벤토나이트에 탄소강, 스텐레스 강, 구리 시편을 넣고, $70^{\circ}C$, 아르곤 분위기에서 530일 경과한 후 시편의 표면 변화 (그림 1) 및 무게 감소를 측정하였다. 철 부식시편은 검정색의 철 화합물 층으로 덮여 있었으며, 구리표면에는 노란색의 부식층이 형성되었는데, 이를 XRD로 분석한 결과 $Cu_2O$로 판명되었다. 그러나 $700^{\circ}C$에서 각각 0, 24, 96시간동안 예민화시킨 스텐레스 강 시편들은 모두 초기상태 그대로 광택을 유지하고 있었으며, XRD에서 다른 화합물의 형성을 발견할 수 없었다 (그림 2). 시편의 무게 감소가 균일부식에 기인한 것으로 가정하여 환산한 결과, 구리와 스텐레스 강 모두 0.3~0.4 $\mu\textrm{m}/yr$의 부식속도를 나타내었다. 그러나 구리는 부식생성물이 표면에 부착되어 있기 때문에 실제 부식두께는 이 값보다 더 클 것으로 생각된다. 용기가 초기 530일간과 같은 속도로 처분용기의 부식이 진행된다면 한국에서 기준처분 개념으로 삼고 있는 50mm 두께의 내부식성 외벽 금속용기는 적어도 만년이상 견딜 수 있을 것으로 추정된다. 한편, 검정색 부식층을 제거한 무게감소로부터 계산한 철의 부식속도는 구리의 약 30배에 해당하였다. 금속 재질의 정확한 부식 거동을 파악하기 위해서는 보다 장기간의 실험이 요구된다. 시험법 선정에 각계(규제기관, 학계, 발전소 현장 및 산업계 등) 전문가로부터 기술자문회의를 통하여 자문 의견을 받기로 하였다. 특히 현재 폐기물 인수 기술기준치가 설정된 국가의 시험법을 심층 있게 검토하기로 하였다.검토하기로 하였다. 혹은 수성주변 환경이 지배하는 산악이나 구릉지에서 흔히 나타나는 침엽수-낙엽활엽수의 혼합림 식생상태를 잘 대변해 주고 있는 것으로 판단된다. 끝으로, 의림지 호저 퇴적층 중에서 인위적인 교란흔적이 없는 암회색 유기질 니층에 대한 탄소연대측정 결과, 제1호공 12번 시료에서 950$\pm$40 years B.P을 얻었으며, 제3-1호공에서도 아래로 내려가면서 8, 10, 11번 시료에 대하여 500$\pm$30 years B.P, 650$\pm$30 years B.P, 800$\pm$40 years B.P의 연대측정 결과를 획득하였다. 이상과 같은 의림지 호저 퇴적층의 형성환경과 형성시기 연구를 통하여 의림지의 제방축조의 최초시기를 해석해 보면, 의림지의 제방은 적어도 과거 약 827년 전에서 866년 전에는 이미 축조되어 있었음을 알 수 있다. 과거 제천 일대에 살았던 옛사람들이 의림지 하류의 곡지중앙과 고기 충적선상지에 대한 관계용 용수조달의 필요성에 부응하여 상류부 곡지하천의 자연입지 환경을 최대한 이용하여 축조한 것으로 판단된다..준비하였다.전류를 구성하는 주요 입자의 에너지 영역(75~l13keV)에서 가장 높은(0.80) 상관계수를 기록했다. 넷째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰 자기폭풍일수록 현저했다. 주상에서 관측된 이러한 특성은 서브스톰 확장기 활동이 자기폭풍의 발달과 밀접한 관계가 있음을 시사한다.se that were all low

• Journal of the Korean Society of Clothing and Textiles
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• v.31 no.12
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• pp.1682-1688
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• 2007

The effects of cultivar and DAPs on the lignin content and dyeability of the kenaf fibers were investigated. Four kenaf fiber samples were prepared from two cultivars, Tainung 2 and Everglades 41, and their 60 and 120 DAPs(days after planting) for the experiments. The lignin contents of the kenaf fibers of Tainung 2(T2) and Everglades 41(E41) were $11.29{\sim}12.78%$. Both T2 and E41 kenaf fibers had comparable amount of lignin, and klason lignin of the fibers was $2.5{\sim}3$ times as much as much as acid-soluble lignin. In both T2 and E41, 120 DAPs kenaf have 1% more lignin than 60 DAPs kenaf. The moisture regains of the four kenaf fiber samples were almost the same as $10.25{\pm}0.05%$. The absorbances of residual solution after dyeing for $1{\sim}180$ minutes with Red 81 at maximum wavelength 520 nm and Green 26 at 600 nm were measured. Comparing to Green 26, the dyeing rate of Red 81 was rapid and equilibrium state was reached in 12 minutes. The CIE $L^*,\;a^*, \;b^*,\;{\Delta}E$ and K/S values of the kenaf fibers dyed with Red 81 and Green 26 were measured as well. The dye exhaustion ratio of 60 DAPs kenaf was higher than that of 120 DAP.