• Title/Summary/Keyword: 스파

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Immunohistochemical Localization of 36 and 29 kDa proteins in sparganum (면역조직염색법으로 관찰한 스파르가눔 층체에서의 36, 29 kDa 항원 단백질의 생성위치)

  • Kim, Lee-Su;Kong, Yoon;Kang, Shin-Yong;Cho, Seung-Yull
    • Parasites, Hosts and Diseases
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    • v.30 no.1
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    • pp.25-32
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    • 1992
  • Antigenic proteins of 36 and 29 kDa were localized in Spirometra mansoni plerocercoid (sparganum) immunohistochemically by avidin biotin complex (ABC) staining. When polyclonal antibodies such as BALB/c mouse serum immunized with crude saline extract of sparganum or confirmed sparganosis sera were reacted as pri-mary antibodies, the positive chromogen (3-amino, 9-ethylcarbazole) reactions were recognized at syncytial tegument, tegumental cells, muscle and parenchymal cells and lining cells of excretory canals. A monoclonal antibody(MAb) which was reacting to 36 and 29 kDa proteins in the extract of the worm was localized at the syncytial tegument and tegumental cells. The present results suggested that the potent antigenic proteins of 36 and 29 kDa in sparganum were produced at the tegumental cells and transported to the syncytial tegument.

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Infectivity of the sparganum treated by praziquantel, gamma-irradiation and mechanical cutting (프라지콴텔, 감마선 조사 및 기계적 절단으로 처리한 스파르가눔의 마우스에 대한 감염력)

  • 손운목;호성태
    • Parasites, Hosts and Diseases
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    • v.31 no.2
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    • pp.135-140
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    • 1993
  • An experimental study was performed to observe the infectivlty of sparganum (plerocercold of Spirometra erinncel) treated by prazlquantel, gamma-irradiation and mechanical cutting. The spargana were obtained from the naturally Infected European grass snake, Rhabdophis tigrina, or from the expenmentally infected mice. A total of 83 mice (ICR straine were divided Into 3 experimental groups by the source of the damage, fed each UH 5 spargana, and sacnaced 1 month later for worm recovery. In the praziquantel group, the worms were incubated In the concentration of 10㎍/ml (control: Tyrode for 4 hours) for 0.5. 1.2 and 4 hours at 36℃. and fed to mice. The recovery rate from mice in prazlquantel group was not different from that (80%) of control poop and in the range of 76-100%. In the gamma-Irradiation group, the worms were irradiated by 10-1000 Gy with Csl37. The average recovery rates of 69-100% were not different from that of control up to 100 Gy. The rate was 56% under 150 Gy, and 5% by 1000 Gy. In the mechanical cutting group, the worms were cut at 0.5, 1, 2 and 3 m from the anterior end of the scolex. The average recovery rates In each group were 70-90% and that of control was 90%. The present folding suggests that the sparganum be highly resistant to prazlquantel, gamma- irradiation and mechanical cutting. The vitality center of the sparganum must be at the anterior end of Its scolex.

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Isolation and Partial Characterization of Cysteine Proteinase from Sparganum (스파르가눔 총체에서 분리한 cysteine proteinase의 정제 및 부분 특성)

  • 송철용;최동호
    • Parasites, Hosts and Diseases
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    • v.30 no.3
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    • pp.191-200
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    • 1992
  • A proteolytic enzyme was purified from the tissue extract of spargana (plerocercoids of Spirometra erinacei) by DEAE-Trisacryl M ion exchange chromatography and thiopropyl-sepharose affinity chromatography resulted in a 21-fold purification. The proteinase activity was assayed with a synthetic fluorescent substrate, carbobensoxy-phenylalanyl-7-amiso-4-trifluoromethyl-coumarin. SDS-polyacplamide gel electrophoresis of the purified materials revealed a single 28,000 dalton band. Inhibitor profiles of the band indicated that it belonged to cysteine endopeptidases. It exhibited identical pH curves with optimum at pH 5,5, and 50% activity from pH 4.7 to 8. It could completely degrade collagen chains to three identical products. It also showed some activity on hemoglobin. Furthermore, the band on immunoblots was reactive to the sera of sparganosis patients. These results suggest that the proteolytic enzyme belongs to cysteine proteinase which plays a role in the tissue penetration. Also it may be used as the antigen for diagnosis of active sparganosis.

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Pleural Sparganosis -A case report - (흉막 스파르가눔증 -1예 보고-)

  • Kim Dae-Hyun;Yi In-Ho;Youn Hyo-Chul;Kim Soo-Cheol;Kim Bum-Shik;Cho Kyu-Seok;Kwak Young-Tae;Park Joo-Chul
    • Journal of Chest Surgery
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    • v.39 no.6 s.263
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    • pp.502-504
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    • 2006
  • The major cause of Sparganosis is ingestion of raw snake or frog. The most common clinical manifestation of Sparganosis is subcutaneous moving nodule in abdominal wall, chest wall, thigh, and scrotal area. The most accurate method of diagnosis and treatment for Sparganosis is surgical removal of the parasite. We experienced pleural sparganosis in a 70-years-old male patient, and report it with review of literatures.

Effect of Maesil (Prunus mume) Juice on the Alcohol Metabolizing Enzyme Activities (매실즙이 알코올대사 효소활성에 미치는 영향)

  • Hwang, Ja-Young;Ham, Jae-Woong;Nam, Sung-Hee
    • Korean Journal of Food Science and Technology
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    • v.36 no.2
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    • pp.329-332
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    • 2004
  • Changes in activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in vitro were examined by measuring maximum absorbances of ADH and ALDH at 340 nm to determine influence of Maesil (Prunus mume) on alcohol metabolism. Facilitating rates of ADH activity were 137.92, 131.58, 152,96, 218.70, 111.76, and 144.27% in Maesil juice, 5, 10, and 15% GMT, and 0.5 and 1.0% aspartic acid, respectively, ALDH activity increased in the order of Maesil juice > ALDH > GMT > aspartic acid, and facilitating rate of ALDH activity in Maesil juice was the highest at 976.44%. These results indicate alcohol metabolizing activity can be enhanced by Maesil juice.

Preparation of damage-less SnO$_{2}$ thin films by RF magnetron sputtering with oxide target (산화물 target의 RF마그네트론 스파터링에 의한 비손상 SnO$_{2}$박막의 제조)

  • 신성호
    • Electrical & Electronic Materials
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    • v.9 no.5
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    • pp.490-497
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    • 1996
  • RF마그네트론 스파터링에 의한 $sSnO_2$ 박막을 증착한 결과 작동조건에 따라 표면에서 막손상이 발생하였는데, 이는 target에서 생성된 높은 에너지의 고속입자가 기판에서 직접 충돌로 일어나며, 마그네트론의 자계분포와도 관계된다. 또한 기판의 위치에 따라 박막의 전기적, 구조적 특성이 급격히 변하는데 본 논문은 중심자석의 세기와 RF power, 가스압력, 그리고 기판온도 등의 스파터링 작동조건을 변화시키면서 박막의 비손상을 검토하였고, 물성특성을 평가하였다. 실험결과로 부터 기판의 외축부에서 제작된 박막은 전반적으로 막손상이 없었고, 특히 target의 중심자석을 Cobalt로 설치하고 15 mTorr의 가스압력과 50 W의 RF power로 한 경우 가장 우수한 특성을 가진 박막을 얻을 수 있었다. 추가로 막손상을 방지하고자, 환원형의 masking glass를 target위에 설치하였는데, 고에너지 입자의 직접 충돌을 확실히 차단할 수 있었으며, 비저항율도 target의 부식부위 (erosion)에 대응하는 부분에서 100배 정도로 개선하였다.

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A systolic Array to Effectively Solve Large Sparce Matrix Linear System of Equations (대형 스파스 메트릭스 선형방정식을 효율적으로 해석하는 씨스톨릭 어레이)

  • 이병홍;채수환;김정선
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.17 no.7
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    • pp.739-748
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    • 1992
  • A CGM iterative systolic algorithm to solve large sparse linear systems of equations is presented. For implementation of the algorithm, a systolic array using the stripe structure is proposed. The matrix A is decomposed into a strictly lower triangular matrix, a diagonal matrix, and a strictly up-per triangular matrix, and the two formers and the tatter· are concurrently computed by different linear arrays. Hence, the execution time of this approach Is reduced to half of the execution time of the that a linear array is used. computation of the Irregularly distributed sparse matrix can be executed effectively by using the stripe structure.

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An Implementation of Photovoltaic Web Monitoring System based Web Services (웹서비스 기반의 태양광 발전 웹모니터링 시스템 구현)

  • Yoo, Jea-Kyu;Ryu, Yeong-Hyeon;Kim, Young-Roc
    • Proceedings of the Korea Information Processing Society Conference
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    • 2010.11a
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    • pp.1381-1383
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    • 2010
  • 웹서비스는 표준 프로토콜인 HTTP를 이용하기 때문에 방화벽의 제약이 없어서 어디서나 실시간으로 통신하여야 하는 모니터링 시스템 특성상 꼭 필요한 기술이다. 또한, XML로 기술된 SOAP 메시지는 플랫폼 독립적이고, 시스템 확장과 통합에 유리하다. 본 논문에서 구현한 웹모니터링 시스템은 웹서비스를 이용하여 설치가 용이하고, 인터넷을 통해 원격지의 태양광 발전 시스템을 관리, 감시하는데 제약이 없으며, 신재생에너지 산업 통합에 적용될 수 있다.

Assessment of Dietary Consumption Patterns of Aspartame and Acesulfame K in the Korean Population (한국인의 아스파탐과 아세설팜 칼륨의 식이 섭취 수준 평가)

  • Suh, Hee-Jae;Choi, Jinhee;An, Duek-Jun;Choi, Sunghee;Kim, Da Young;Kim, Ae Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.604-611
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    • 2014
  • The purpose of this study was to assess the consumption of aspartame and acesulfame K, the most frequently utilized artificial sweeteners in Korea. The contents of aspartame and acesulfame K in processed foodstuffs were analyzed by HPLC, and daily intakes according to sex and age were estimated by applying the consumption data from the Korea National Health and Nutrition Examination Survey. Estimated daily intakes of aspartame and acesulfame K were 58.37 ${\mu}g/kg$ bw/day and 14.23 ${\mu}g/kg$ bw/day, respectively, in 2012. These amounts constituted 0.15% and 0.09% of the ADI (Acceptable Daily Intake) established by the JECFA (FAO/WHO Joint Expert Committee on Food Additives). Estimated daily intakes of aspartame and acesulfame K in the 95 percentile consumption group were 2,510.48 ${\mu}g/kg$ bw/day and 761.92 ${\mu}g/kg$ bw/day, respectively, and intake levels were 6.28% and 5.08% of the ADI, respectively. In conclusion, daily intake levels of artificial sweeteners were evaluated to be at safe levels.

Quality Characteristics of Vanilla Sauce with Various Sweeteners (감미료의 종류를 달리한 바닐라 소스의 품질특성)

  • Lee, Dong-gue;Kim, Ki-bbeum;Park, Ki-hong;Choi, Soo-keun
    • Culinary science and hospitality research
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    • v.22 no.7
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    • pp.36-46
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    • 2016
  • The study examined the kinds of sweeteners(xylitol, sorbitol, acesulfame K, aspartame, stevioside) instead of sugar for vanilla sauce to satisfy customers' health needs. According to the results, the sauce with sugar had lowest salinity, highest sugar content, and sauce with aspartame had lowest sugar content. pH were highest in sauce with sorbitol, with aspartame were lowest. L-value, a-value was the highest in sauce with aspartame, while b-value was the lowest. The moisture content was the lowest, while viscosity was the highest in the sauce with xylitol. In a sensory evaluation, texture and overall preference was the highest in vanilla sauces with aspartame which have proper color intensity and strong flavor in mouth feel. The result indicated that health-oriented consumers and satisfy the health of modern people's needs when using the vanilla sauce with aspartame instead of sugar. It is also advised to vanilla sauce with aspartame for preventing adult disease and using the diets for patient.