• Korean Security Journal
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• no.33
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• pp.51-80
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• 2012

This study is to summarize main contents which was mentioned by Beomseok Kim' doctoral dissertation. The purpose of this study focuses on presenting the outplacement countermeasure and retention level examination analysis about outplacement competency of special security government official through implement of questionnaire method. The questionnaire for retention level examination including four groups of outplacement competency and twenty subcategories was implemented in the object of six hundered persons relevant to outplacement more than forty age and five grade administration official of special security government officials, who have outplacement experiences as outplacement successors, outplacement losers, and outplacement expectants, in order to achieve this research purpose effectively. The questionnaire examination items are four groups of outplacement competency and twenty subcategories which are the group of knowledge competency & four subcategories including expert knowledge, outplacement knowledge, self comprehension, and organization comprehension, the group of skill competency & nine subcategories including job skill competency, job performance skill, problem-solving skill, reforming skill, communication skill, organization management skill, crisis management skill, career development skill, and human network application skill, the group of attitude-emotion competency & seven subcategories including positive attitude, active attitude, responsibility, professionalism, devoting-sacrificing attitude, affinity, and self-controlling ability, and the group of value-ethics competency & two subcategories including ethical consciousness and morality. The respondents highly regard twenty-two outplacement competency and they consider themselves well-qualified for the subcategories valued over 4.0 such as the professional knowledge, active attitude, responsibility, ethics and morality while they mark the other subcategories below average still need to be improved. Thus, the following is suggestions for successful outplacement. First, individual effort is essential to strengthen their capabilities based on accurate self evaluation, for which the awareness and concept need to be redefined to help them face up to the reality by readjusting career goal to a realistic level. Second, active career development plan to improve shortcoming in terms of outplacement competency is required. Third, it is necessary to establish the infrastructure related to outplacement training such as ON-OFF Line training system and facilities for learning to reinforce user-oriented outplacement training as a regular training course before during after the retirement.

• Clinical and Experimental Reproductive Medicine
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• v.36 no.4
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• pp.283-292
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• 2009

Objective: Human embryonic stem cells (hESCs) can proliferate indefinitely and differentiate into all kinds of cell types in vitro. Therefore, hESCs can be used as a cell source for cell-based therapy. Transduction of foreign genes to hESCs could be useful for tracing differentiation processes of hESCs and elucidation of gene function. Thus, we tried to introduce enhanced green fluorescent protein (eGFP) gene to hESCs, XX and XY cell lines in this study. Methods: Lentivirus containing eGFP was packaged in 293T cells and applied to hESCs to transduce eGFP. Expression of transduced eGFP was evaluated under the fluorescence microscope and eGFP positive population was analyzed by FACS. Expression of undifferentiation state markers such as Oct4, Nanog, SSEA4 and Tra-1-81 was examined by RT-PCR and/or immunofluorescence in eGFP-hESCs after transduction. In addition, the ability of eGFP-hESCs to form embryoid bodies (EBs) was tested. Results: eGFP was successfully transduced to hESCs by lentivirus. eGFP expression was stably maintained up to more than 40 passages. eGFP-hESCs retained expression patterns of undifferentiation state markers after transduction. Interestingly, disappearance of transduced eGFP was notably observed during spontaneous differentiation of eGFP-hESCs. Conclusion: We established eGFP expressing hESC lines using lentivirus and showed the maintenance of undifferentiation characteristics of these eGFP-hESCs. This reporter-containing hESCs could be useful for tracing the processes of differentiation of hESCs and other studies.

• Journal of Animal Science and Technology
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• v.52 no.1
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• pp.29-36
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• 2010

This experiment was conducted to evaluate the influence of phase feeding methods on growth performance, carcass characteristics, pork quality, and economical efficiency in growing-finishing pigs. A total of 120 crossbred pigs ([Yorkshire ${\times}$ Landrace] ${\times}$ Duroc), average initial body weight 25.23 ${\pm}$ 2.66 kg, were allotted to 5 treatments by body weight and sex in a randomized complete block (RCB) design. Each treatment had 6 replicates with 4 pigs per pen. The treatments were 1) A (fed late weaner feed during whole phase), 2) B (fed late weaner feed in growing phase and grower feed in finishing phase), 3) C (fed grower feed during whole phase), 4) D (fed grower feed in growing phase and early finisher feed in early and late finishing phase), 5) E (fed grower feed in growing phase, early finisher feed in early finishing phase and late finisher feed in late finishing phase). Commercial diets bought from private feed company were used for this experiment. During the early growing phase (6 wks), treatments A and B showed higher growth performance than that of other treatments (P<0.05). However, growth performance tended to be similar among treatments at 10 wks after feeding and there was no difference in time of market weight (13 wks). Moreover, blood urea nitrogen (BUN) concentration was lowered in treatment E than other treatments during whole experimental period (P<0.01). Carcass characteristics and pork quality were measured when body weight of pigs reached at 110.33 ${\pm}$ 2.1 kg. Days to market weight (110 kg), quality grade, pork color, and pork pH did not show any significant difference among treatments. These results suggested that phase feeding based upon growth stages of pigs showed no detrimental effects on growth performance and the pork quality. Moreover, feed cost was saved approximately 37% by phase feeding method without delaying of days to market weight compared to general conventional feeding method (B) of swine farm in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.2
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• pp.195-202
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• 1995

This experiment was performed to characterize the optimum water table level for the growth and yield of barley(var. Olbori) and wheat(var, Grumil), Olbori and Grumil were grown in the 550 liter plastic pot filled with silt loam or sandy loam, During the whole growth period, the water table adjusted to be 20, 30, 40, 50, and 70cm, Higher water table was resulted in the decrease in plant height and top dry weight, but in the increase of the ratio of top to root dry weight, especially in barley, This suggested that high water table level affected more the growth of top than that of root, The number and area of green leaves were decreased as the water table was higher than 30 to 40cm at the late growth period(May 18, 1993), The largest number and area of green leaves were shown at 50cm of water table in sandy loam and at 70cm in silt loam, As the water table was high, the leaf chlorophyll content was low, And barley was affected more significantly than wheat by soil texture, The photosynthetic activity was decreased remarkably at 20cm water table, Heading period was 2 to 3 and 4 days earlier at the 20cm water table of sandy loam in barley and wheat, respectively, However this earlier heading was not shown in silt loam, Grain filling was accelerated 5 to 7 days earlier in barley and 10 days in wheat grown at 20cm water table, The highest yield was present at 50 and 70cm water table, The yield was decreased remarkably at 20cm water table, resulting that yield reduction ratio of barley was 71.1% and 72, 2%, and that of wheat was 41.0% and 60, 0% in sandy loam and silt loam, respectively, High water table decreased the number of spike per unit area, but increased the seed weight per spike in barley, However, High water table reduced the seed weight per spike in wheat. There was significant correlation between yield and leaf chlorophyll content in wheat and barley, Yield was correlated significantly with green leaf area in barley, and with top dry weight, ratio of top to root dry weight chlorophyll content and photosynthetic activity in wheat. The optimum water table was 50 to 70cm in wheat and barley, They grew fairly well at 30cm water table of sandy loam, and at 40cm of silt loam.

• Culinary science and hospitality research
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• v.18 no.2
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• pp.134-148
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• 2012

This study was conducted to investigate the state of cooking activities at home and at school among the elementary school 6th graders in Gyeongbuk province. For 'interest in cooking', 86.9% of the subjects answered 'very interested,' and the answers were significantly different according to sex(male 45.6%, female 77.8%, p<0.01). Above 70.0% of the subjects answered that they could cook by themselves and the responses were significantly different according to sex(male 35.3%, female 66.7%, p<0.01). The children had experiences of chopping(64.8%), slicing round(62.3%), cutting dice(62.3%), cutting julienne(53.3%), cutting diagonally(45.1%), slicing semicircle(34.4%), and cutting paysanne(22.1%). 93.4% of the subjects had cooking experience at home and most parents(95.1%) were positive to children's cooking at home. The reasons for cooking at home were 'like to cook'(55.3%), 'to satisfy hunger'(17.5%), 'to eat foods that I want'(14.0%), etc. The only 10.7% of the subjects answered 'difficult' or 'very difficult' for the cooking classes at school. The 62.3% of the subjects have cooked the foods again at home that they learned at school, and the responses were significantly different according to sex(p<0.05). The foods that the children wanted to cook at school were pizza, spaghetti, Tteokbokki, hamburgers, etc. The children answered that they had difficulties in 'seasoning'(34.4%) and 'cleaning up after cooking'(21.5%). What the children wished about the cooking class of their schools included 'to cook more often'(35.8%), 'to cook more diverse foods'(29.7%), 'to have the school equipped with cooking tools and facilities'(14.8%), etc. Therefore, the cooking classes for the elementary school students need to be planned considering these results about children's cooking abilities, skills and demands.

• Journal of Korean Society of Forest Science
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• v.84 no.4
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• pp.424-431
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• 1995

This study was conducted to evaluate resistance and physiological responses of Zelkova serrata Makino seedlings to ozone in open-top chamber. One-year-old seedlings of Zelkova serrata were planted in pots in April and grown in greenhouse until August. The plants were transferred into two out-door open-top chambers with a dimension of 2.0 m in diameter and 2.0 m in height. First chamber served as a control and was supplied with ambient air. Ozone was added to the second chamber for 5 hours per day(10.00 AM-15.00 PM) for 23 consecutive days at 0.1 ppm. Each chamber housed 70 pots. Every two, three or five days after initiation of exposure, ten pots were randomly removed from the chamber and determined for the contents of chlorophyll a, b, total chlorophyll and ${\beta}$-carotene in the leaves. Photosynthesis and dark respiration were estimated by measuring $CO_2$ absorption in a gas exchange chamber and oxygen absorption by oxygen monitoring system, respectively. Superoxide dismutase(SOD) activity in the leaves was assayed by a xanthine oxidase method. First visible injury of translucent(water-soaked looking) spots appeared on the leaves 14 days after the initial exposure, and ozone accelerated senescence of old leaves. Contents of chlorophyll a and b decreased by 17%, and 31%, respectively, in ozone treatment two days after exposure. The decrease in chlorophyll b was greater than that of chlorophyll a. Content of ${\beta}$-carotene in ozone treatment decreased by 25% two days after initiation of exposure, but the reduction was recovered with time. Photosynthesis decreased by 45%, and the respiration increased by 28% in the ozone treatment. SOD activity started to increase 4 days after beginning of exposure and increased by 285% 7 days after exposure, and decreased to the level below the control treatment with the advancement of the visible injury.

• Journal of Embryo Transfer
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• v.26 no.1
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• pp.65-70
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• 2011

This study was conducted to investigate the survival rate of AndroMed and Tris-egg yolk extender for cryopreservation of Korean Native Bull Semen (Chick Cow). Semen was collected from a Korean Native Bull Semen over 3 year's old. The semen was diluted 1:1 by AndroMed and Tris-egg yolk extender. The pellet was diluted to final sperm concentration of $5{\times}10^7$ cell/ml by doubling in every 10 minutes at $4^{\circ}C$ cold chamber. The semen was equilibrated for 1 hrs at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm of liquid nitrogen for 5 minutes. And then the frozen straw was plunged to LN2. The presented straws were examined the viability and motility after thawed at $37^{\circ}C$ water bath. The survival rates was significantly higher (p<0.05) in Tris-egg yolk extender than AndroMed extender ($89.7{\pm}19.8$ vs. $73.4{\pm}11.2$). However, motility was no significant differences ($78.4{\pm}18.7$ vs. $67.9{\pm}14.6$). Survival rate in time of equilibration between visual and CASA program had higher in 2 h ($86.33{\pm}9.4$ vs. $92.32{\pm}12.4$) than in 5 h ($78.20{\pm}7.8$ vs. $88.28{\pm}13.1$) 15 h ($65.24{\pm}6.6$ vs. $76.48{\pm}17.3$) 20 h ($56.26{\pm}4.6$ vs. $67.73{\pm}18.4$). The development rates to cleavage was higher in Tris-egg yolk extender than AndroMed extender (82.2% vs. 81.7%). Similarly, the development rates to blastocyst was significantly higher (p<0.05) in Tris-egg yolk extender than AndroMed extender (42.3% vs. 29.6%). In conclusion, the obvious impact of this study will be its practical application to improve viability and fertilizing ability of cryopreserved spermatozoa used for in vitro fertilization (IVF) and AI, Which in turn will be beneficial to animal genetic resources conservation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.466-476
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• 2006

The objective of this study was to compare and analyze the acceptability and consumption attitude for soy foods between Korean and Canadian university students as young consumers. This survey was carried out by questionnaire and the subjects were n=516 in Korea and n=502 in Canada. Opinions for soy foods in terms of general knowledge were that soy foods are healthy (86.5% in Korean and 53.4% in Canadian) or neutral (11.6% in Korean and 42.8% in Canadian), dairy foods can be substituted by soy foods (51.9% in Korean and 41.8% in Canadian), and soy foods are not only for vegetarians and milk allergy Patients but also for ordinary People (94.2% in Korean and 87.6% in Canadian). In main sources of information about soy foods, the rate by commercials on TV, radio or magazine was the highest (58.0%) for Korean students and the rate by family or friend was the highest(35.7%) for Canadian students. In consumption attitude, all of Korean students have purchased soy foods but only 55.4% of Canadian students have purchased soy foods, and soymilk was remarkably recognized and consumed then soy beverage and margarine in order. 76.4% of Korean students and 65.1% of Canadian students think soy foods are general and popular and can purchase easily, otherwise, in terms of price, soy foods were expensively recognized as 'more expensive than dairy foods' was 59.1% (Korean) and 54.7% (Canadian), and 'similar to dairy foods' was 36.8% (Korean) and 39.9% (Canadian). Major reasons for the rare consumption were 'I am not interested in soy foods' in Korean students (27.3%) and 'I prefer dairy foods to soy foods' in Canadian students (51.7%). However, consumption of soy foods in both countries are very positive and it will be increased.

• Korean Journal of Food Science and Technology
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• v.39 no.2
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• pp.138-145
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• 2007

The effects of amylopectin and hydrocolloid (locust bean gum and guar gum) content on wheat flour dough and noodle properties were investigated. As the amount of amylopectin increased, the water absorption rate (farinograph), the tension (tension test), the gel stability (freeze-thawing treatment), and the springiness and the cohesiveness (TPA) increased, but the pasting temperature (RVA), the lightness and yellowness (color measurement), and the hardness (TPA) tended to decrease. In sensory evaluations, the scores for cohesiveness, springiness, and acceptability of cooked noodle increased as the proportion of amylopectin increased. The proper combination of amylose/amylopectin ratio and hydrocolloids improved the freeze-thaw stability and the sensory acceptability of wheat flour dough and noodle.

• Korean Journal of Poultry Science
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• v.43 no.1
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• pp.39-45
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• 2016

This study was carried out to evaluate performance and uniformity of White Korean native ducks collected from two areas in Korea. White ducklings were selected from all ducklings that were hatched from hatchery eggs of Korean native ducks. A total of two hundred forty white ducklings (1-d-old) were used in this work and were divided into 4 groups (4 replicates/group, 15 birds/replicate) with $2{\times}2$ factors (2 groups, J, H; genders, male and female). Weekly body weight of H groups was higher than that of J groups (P<0.05). Also, weekly body weight of male ducks was higher compared to that of female at 6~8 wk old (P<0.05). Uniformity of J groups was higher than that of H groups at the age of 1 wks old (P<0.05), and that of female duck was higher than that of male at the age of 6 wks old (P<0.05). Body weight gain of H group was higher than that of J group (P<0.05), and that of male duck was higher compared to that of female at 6~7 wks old (P<0.05). Feed intake of H group was higher compared to that of J group (P<0.05), but there was no significant difference between male and female on feed intake. On feed conversion ratio, J group was higher than H group at 4~5 wks, 7~8 wks and 0~8 wks old (P<0.05), and feed conversion ratio of male duck was higher than that of female at 1~2 wks, 5~6 wks and 6~7 wks old. Finally, these results was tended to show that performance of H groups was higher than that of J groups. Further researches need to carry out to commercial White Korean native ducks on performance and uniformity as well as meat quality and genetic characteristic.