• Journal of Life Science
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• v.20 no.4
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• pp.607-613
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• 2010

Osteoporosis is a disease involving a decrease in bone mineral density and an increased risk of fractures. The MC3T3-E1 pre-osteoblastic cell line is a well-accepted model of osteogenesis in vitro. Pine needles have long been used as a traditional health-promoting medicinal food in Korea. In this study, MTT assay, the alkaline phosphatase (ALP) activity and collagen synthesis of osteoblast cells were investigated to determine the effects of pine needle extracts on cell proliferation and differentiation. Pine needle extracts were prepared using hexane, ethanol and water. The effects of the pine needle extracts were examined by comparing the results with those of commercial agents, such as proanthocyanidin. The MC3T3-E1 cells exposed to proanthocyanidin showed increased proliferation in a concentration-dependent manner. The cells exposed to the hexane extract showed a similar increase in proliferation to that observed with proanthocyanidin. The hexane extract showed the highest ALP activity. Moreover, a supplement of pine needle extracts induced collagen synthesis in MC3T3-E1 cells. The pine needle extract produced the highest level of collagen synthesis at concentrations of $10{\sim}50\;{\mu}g/ml$. These results indicate that pine needle extracts have an anabolic effect on bone by promoting osteoblastic differentiation, and may be used in the treatment of common metabolic bone diseases.

• Journal of Animal Science and Technology
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• v.45 no.6
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• pp.1047-1060
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• 2003

To investigate the effects of conjugated linoleic acid added diet feeding on CLA accumulation and quality characteristics of pork meat. The CLA used to add in diet was chemically synthesized by alkaline isomerization method with corn oil. Pigs were divided into 5 treatment groups(4 pigs/group) and subjected to one of five treatment diets(0, 1.25% CLA for 2 weeks, 2.5% CLA for 2 weeks, 1.25% CLA for 4 weeks and 2.5% CLA for 4 weeks, CLA diets; total fed diets) before slaughter. Pork loin were collected from the animals(110 kg body weight) slaughtering at the commercial slaughter house. Pork loin meat were aerobic packaged and then stored during 2, 5, 8, 11 and 14 days at 4$^{\circ}C$. Samples were analyzed for shear force value, texture, TBARS, fatty acid composition, cholesterol and CLA content. CLA treatment groups showed significantly(p〈0.05) higher shear force value compared to those of control group at 11, 14 days of cold storage. All treatments were decreased significantly as the storage period passed. There was a not significantly difference in texture between control and CLA treatment groups. All CLA treatment groups showed significantly(p〈0.05) lower TBARS value than the control. TBARS value was increased significantly during storage in all treatment. CLA treatment groups showed significantly(p〈0.05) lower cholesterol content compared to those of control group. As dietary CLA was increased in feed, the content of CLA was increased, but the control was almost not detected. The contents of CLA were not significantly changed during chilled storage for 14 days. In the change of fatty acid composition, the contents of oleic, linoleic and arachidonic were decreased by dietary CLA-supplementation, whereas the increase level of CLA-supplementation resulted in the higher palmitic and stearic acid. In all results, CLA could be accumulated in pork meat and its antioxidant capability has been indicated. It was suggested that dietary CLA-supplementation could be produced high quality pork.

• Asian Journal of Turfgrass Science
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• v.22 no.1
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• pp.75-84
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• 2008

This study was carried out to examine the effects of cornstarch-based absorbent polymer (CAP) on the growth of cool season turfgrasses in sand-based soil mixture. Kentucky bluegrass + perennial ryegrass mixtures seeded at May 18 in 2006 on sand-based soil mixture. Sand + peat (5%, v/v), sand + CAP $20g{\cdot}m^{-2}$, sand + CAP $20g{\cdot}m^{-2}$ + peat (5%, v/v), and sand + CAP $40g{\cdot}m^{-2}$ + peat (5%, v/v) mixtures were compared. Ground coverage of sand + CAP $20g{\cdot}m^{-2}$ + peat (5%, v/v), and sand + CAP $40g{\cdot}m^{-2}$ + peat (5%, v/v) treatments showed 50% at a month after seeding. But the coverage of sand + peat (5%, v/v), sand + CAP $20g{\cdot}m^{-2}$ resulted in 36.7%. Mixing of CAP with sand was considered to be efficient method for increasing ground coverage as much as peat. Dry weight of turfgrass tiller at sand + CAP $20g{\cdot}m^{-2}$ + peat (5%, v/v), and sand + CAP $40g{\cdot}m^{-2}$ + peat (5%, v/v) were also significantly higher than sand + peat (5%, v/v), sand + CAP $20g{\cdot}m^{-2}$ mixtures at a month after seeding. Soil water retention at the sand + CAP $20g{\cdot}m^{-2}$, sand + CAP $40g{\cdot}m^{-2}$ + peat (5%, v/v) mixing were lower than sand + peat (5%, v/v) and sand + CAP $20g{\cdot}m^{-2}$ + peat (5%, v/v) during the dry periods. From the results, the mixing of CAP with sand is useful to increased ground coverage of kentucky bluegrass and perennial ryegrass.

• Korean Journal of Food Science and Technology
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• v.45 no.5
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• pp.583-589
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• 2013

We investigated the delay of kimchi fermentation by the addition of plant extracts. Fifteen plant extracts were screened for inhibitory activity aginst Lactobacillus plantarum by using an agar well diffusion assay, and determined the minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) were determined. The lowest MIC for grapefruit seed extract (GFSE; 0.0313 mg/mL) was determined, followed by Caesalpinia sappan L. extract (CSLE; 0.25 mg/mL), and oregano essential oil (OREO; 1.0 mg/mL). GFSE, CSLE, and OREO were individually added to kimchi, and incubated the samples at 10 for up to 20 days. Results showed that the addition of GFSE (0.3 and 0.5%), CSLE (0.1, 0.3, and 0.5%), or OREO (0.5 and 1.0%) led to a significant increase in the pH of kimchi, and also a significant reduction in the numbers of lactic acid bacteria. Taken together, the addition of natural antimicrobial agents can delay kimchi fermentation.

• Journal of Animal Science and Technology
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• v.54 no.2
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• pp.71-75
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• 2012

The present study data were obtained from 36,894 cows in Korea Animal Improvement Association from 2001 to 2009 which was subjected for ultrasound measurements (eye muscle area, back-fat thickness, marbling score) and descent. Repeated record models were carried out using 7,913 of 36,894 of total animal traits. The ultrasound measured traits and performance test data were used to study the chest girth, body condition score, eye muscle area, back-fat thickness and marbling score with genetic correlation and parameters for the ultrasound measured traits using REMLF90 program. Genetic correlation of eye muscle area with back-fat thickness, marbling score and back-fat thickness with marbling score were noticed in repeated records animal model as 0.69, 0.54, and 0.59, whereas in multiple trait animal model method were 0.07, 0.66, and 0.39, respectively. Repeated records of animal models were used as positive correlation of traits. Multiple trait animal models were used as negative correlation of eye muscle area with marbling score. The analysis on repeat records of animal models using ultrasound measurements about Korean cattle showed positive effects for each traits. In comparison differences between the repeat records of animal models and multiple trait animal models was found with higher traits of her, the heritability and repeatability was found higher in repeat records animal models. In light of these assessments, carcass traits by ultrasound measurements are expected to help and improve an accurate analysis of each trait and if the research analysis using repeat records of animal models continue when we estimate genetic ability of these traits.

• Current Research on Agriculture and Life Sciences
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• v.1
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• pp.179-188
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• 1983

This study was conducted to find out the most fabourable breeding structure for the maximum genetic gain of live weight, the most important economic traits of Korean native cattle, in order to achieve the improvement goals for meat productivity of the native cattle early and effectively. For estimating genetic gain and population mean changes, the following factors were investigated under the assumation that 675,000 heads of over-two-year old cows were maintained each generation and 15% of the cows were culled every year: 1. The proportion of cow population inseminated by A I bulls; 30, 40, 50, 60, 70% 2. The number of semen doses produced from each A I bull per year; 5,000, 7,000, 10,000, 15,000, 20,000 doses. 3. The average body weight of A I bulls; 480, 520, 600, 640, 680, 720kg/18 months of age. The estimated results are summarized as follows: 1. The genetic gain of live weight is affected greately by the levels of A I bulls' body weight and the genetic gain was estimated 28.66~36.31kg per generation. 2. The proportion of genetic gain from sire selection were estimated 80~90%. 3. When the average body weight of A I bulls increase 40kg per generation and more than 50% of cow population is inseminated by A I bulls, then the phenotypic mean value of live weight of bulls at the age of 18 months and heifers at the age of 2 years are expected to be reached 600kg and 520kg in the A I population; 560kg and 480kg in the whole population, respectively, after 5th generation.

• Journal of Korea Foresty Energy
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• v.21 no.2
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• pp.25-33
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• 2002

One of the critical problems to preserve books and documents in libraries and archives is the deterioration. Some of previous results showed that the major cause of paper deterioration was the acid-catalyzed hydrolysis of the cellulose in paper fibres and aging rate of acidic paper was faster than that of alkaline paper. Therefore, It is necessary to remove the acid in the paper for reducing the rate of paper deterioration. It has been reported to extend the useful life of acidic paper by three to five times. Recently, It has been recognized the need for an effective method of deacidifying large quantities of books and document. However, in the previous many reports little attention was paid to the effect of paper additives. In this paper, We carried out experiment about the effect of additives on paper aging and the effect of deacidification by the gaseous ethanolamines (monoehtanolamine, diethanolamine, triehtanolamine). In result, it was found that the strength of aging was in the order of the alum＋rosin＞alum ＞AKD＞ control and the rate of deacidification was in the order of the monoethanolamine＞diethanolamine＞triethanolamine. The treatment with the gaseous ethanolamines caused decreasing of brightness and dropping of fold endurances. However, deacidification by combination treatment of the various gaseous ehtnaolamines prevented from decreasing of brightness and dropping of folding endurances.

• Journal of the Korean Association of Geographic Information Studies
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• v.23 no.3
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• pp.100-119
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• 2020

During recent years, the heat environment and particulate matter (PM₁₀) have become serious environmental problems, as increases in heat waves due to rising global temperature interact with weakening atmospheric wind speeds. There exist urban heat islands and urban pollution islands with higher temperatures and air pollution concentrations than other areas. However, few studies have examined these issues together because of a lack of micro-scale data, which can be constructed from spatial data. Today, with the help of satellite images and big data collected by private telecommunication companies, detailed spatial distribution analyses are possible. Therefore, this study aimed to examine the spatial distribution patterns of urban heat islands and urban pollution islands within Busan Metropolitan City and to compare the distributions of the two phenomena. In this study, the land surface temperature of Landsat 8 satellite images, air temperature and particulate matter concentration data derived from a private automated meteorological observation system were gridded in 30m × 30m units, and spatial analysis was performed. Analysis showed that simultaneous zones of urban heat islands and urban pollution islands included some vulnerable residential areas and industrial areas. The political migration areas such as Seo-dong and Bansong-dong, representative vulnerable residential areas in Busan, were included in the co-occurring areas. The areas have a high density of buildings and poor ventilation, most of whose residents are vulnerable to heat waves and air pollution; thus, these areas must be considered first when establishing related policies. In the industrial areas included in the co-occurring areas, concrete or asphalt concrete-based impervious surfaces accounted for an absolute majority, and not only was the proportion of vegetation insufficient, there was also considerable vehicular traffic. A hot-spot analysis examining the reliability of the analysis confirmed that more than 99.96% of the regions corresponded to hot-spot areas at a 99% confidence level.

• 한국산학경영학회:학술대회논문집
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• 2004.11a
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• pp.101-123
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• 2004

This study examines empirically the significant changes in tax payments when the consolidated tax return is introduced in the future. We estimate the consolidated tax payments under the eight cases which are classified as such : whether only 100% ownership subsidiaries should be included or 80% and over, whether all subsidiaries should be included or only subsidiaries with loss, and whether unrealized profits from intercompany transactions should be excluded or not. After estimating the consolidated tax payments, we test the difference between the consolidated tax payments and the sum of the individual tax payments of the subsidiaries. The results of the test show that the consolidated tax payments are significantly less than the sum of the individual tax payments of the subsidiaries. We interpret that the inclusion of the losses of the subsidiaries in the consolidated tax base makes the tax payment decrease. Based on our analysis about 3.8 billion Won per each parent company would decrease due to the introduction of the consolidated tax return. And we find that under the mandatory consolidated tax return system the significant difference between the consolidated and individual tax payment exists except that the only 100% ownership subsidiaries are included and unrealized profits from intercompany transactions are not excluded. However, when the parent companies have the discretion to select the consolidated subsidiaries, the consolidated tax payments are significantly less than the sum of the individual tax payments of the subsidiaries regardless of the ownership percentage, inclusion of the loss of the subsidiaries and exclusion of the unrealized profits.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.1 no.1
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• pp.117-129
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• 1990

35 children(17 boys, 18 girls) with Recurrent Abdominal pain(RAP) without apparent organic causes, referrd from pediatric department of Seoul National University Children's Hospital were studied during 1988. 7. 1 ${\sim}$ 1989. 2. 28. The aims of this study are to examine clinical characteristics of the patients with RAP, to find possible factors influencing the onset and the course of the disorder, to evaluate the psychological status and the relationship with psychiatric diagnoses. The study subjects were compared with the age and sex matched control subjects on measures of social and school functioning, family-environment, behavior traits by CBCL and parent-environment rating scale. All patients and their mothers were interviewed by a child psychiatrist. The results were as follows ; 1) Abdominal pain was found to be more frequent when with a care taker, and related with traumatic life stresses such as separation from parents, death of family members. 2) Family members of the patients with RAP also had a high rate of a history of gastrointestinal dysfunctions with could not be attributed to organic etiologies. 3) Among 35 patients with RAP, 16 were diagnosed as psychiatric disorders. They were adjustment disorders (4), overanxious disorders (4), depression (2), tic disorders (2), attention deficit disorders (2), separation anxiety disorders (1), enuresis (1). 4) On CBCL, the patients with RAP were more internalized, socially less competent and less active compared with the control subjects. 5) According to the parent-environment rating scale, the patients with RAP had more conflicts with their parents about control issues. Family members of the patients with RAP were socially less competent and less abjustable. The parents of the patients with RAP were more affectionate and spent more time with their children but they were unfair and vague on instruction and discipline.