• Title/Summary/Keyword: 수정란 이식

Search Result 1,795, Processing Time 0.021 seconds

소 수정란의 채란

  • Jeong, Gyeong-Il;Gang, Sang-Won
    • Proceedings of the Korean Society of Embryo Transfer Conference
    • /
    • 1997.10a
    • /
    • pp.28-29
    • /
    • 1997

  • PDF

Studies on nuclear transplantation in mouse embryos II. Developmental potential of nuclei from embryos of different developmental stages (생쥐 수정란의 핵이식에 관한 연구 II. 발달단계별 수정란 핵의 이식후 생존성)

  • Park, Choong-saeng;Choe, Sang-yong;Lee, Hyo-jong;Park, Hee-sung
    • Korean Journal of Veterinary Research
    • /
    • v.30 no.4
    • /
    • pp.355-360
    • /
    • 1990

  • Single nuclei from two-, four- and eight-cell mouse embryos were transplanted into enucleated two-cell embryos by micromanipulation and Sendai virus mediated fusion. The developmental potential of these reconstituted embryos in vitro and in vivo was examined. It was found that the single nuclei which were transplanted to enucleated two-cell embryos were not only able to develop to the blastocyst stage in vitro(two-cell nuclei, 76.5%; four-cell nuclei, 68.4%; eight-cell nuclei, 48.3%), but also able to develop to full term in vivo after transfer to recipient mice(two-cell nuclei, 37.1%; four-cell nuclei, 29.6%; eight-cell nuclei, 16.3%). Although the proportion of live young produced after transfer of nucler of nuclear transplant embryos which received eight-cell nuclei was significantly (p<0.05) reduced, it would be suggested that the overall efficiency in producing identical offspring is greater when eight-cell embryos were selected for nuclear donor than two- or four-cell embryos were selected.

  • PDF

Possibility of Embryo Collection by Repeatable Superovulation in Hanwoo : Case Report (한우에서 반복 과배란처리에 의한 수정란 채란 가능성 : 사례보고)

  • 공일근;정세환;손병훈;이정우;정수룡;오인석;정경일;황금영;배인휴
    • Journal of Embryo Transfer
    • /
    • v.17 no.1
    • /
    • pp.1-5
    • /
    • 2002

  • The purpose of this study was carried out to determine the possibility of continued embryo collection by repeatable superovulation from a Hanwoo elite cow, who was already estimated to an elite cow by DNA analysis. The donor cow was superovulated with Folltropin-V and PG $F_2$$\alpha$ combination method for 5 times, 2 months interval during 10 months. The estrus cows were inseminated with frozen semen 3 times 12 hrs interval. Embryo collection from superovulated cows was flushed non-surgical method at 7 to 8 days after artificial insemination. The superovulated cow was collected from 1 to 5$^{th}$ times as total (14, 4, 10, 6, 9 embryos; total 30 embryos) and transferable embryos (7, 4, 10, 3, 6; Mean$\pm$S.E : 6.0$\pm$2.7 embryos). Seventeen recipients were transferred 24 embryos and then detected to pregnant 10 recipients after embryo transfer (58.8%; 10/17 recipients) The results indicated that the possibility of repeatable superovulation from elite donor cow for embryo transfer could be confirmed in this study, although the replicated number was not enough.

  • PDF KSCI