• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2000.11a
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• pp.27-33
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• 2000

• Journal of Embryo Transfer
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• v.20 no.1
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• pp.9-15
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• 2005

This study was conducted to investigate the effects of various factors of practitioner's techniques such as skill, difficulty, consumed time, implantation depth, bleeding and altering of implant location, on pregnancy and abortion after transfer of in vitro produced Korean Native Cow embryos. The pregnancy rate of skilled transfer $(54.2\%)$ was significantly higher than that of unskilled transfer $(37.9\%)$, of 2/3 $(46.9\%)$ location in uterus was significantly higher than in 1/2 $(39.5\%)$ or 4/5 $(38.0\%)$ uterus, and of altering implantation location $(52.9\%)$ was significantly increased. There were no difference in pregnancy among the groups of difficulty of transfer, consumed time and bleeding of uterus. The abortion rates from skill, difficulty of transfer, implantation depth, consumed time, bleeding of uterus and altering of location were not differ.

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.42-42
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• 2002

본 연구는 한국 재래산양의 핵이식에 있어서 공여핵은 귀세포를, 수핵난자는 소 및 돼지의 난포란을 이용하여 핵이식을 실시하여 복제수정란의 체외발달율과 이종간의 핵이식 가능성을 검토하였다. 공여핵은 재래산양의 귀세포를 채취하여 10% FBS 가 첨가된 TCM-199 배양액으로 체외 배양을 실시하여 monolayar Confluent 형성후 0.25% Trypsin-EDTA을 처리하여 계대배양을 실시하였다. (중략)

• Journal of Embryo Transfer
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• v.22 no.1
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• pp.53-61
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• 2007

This study was investigated factors affecting the pregnancy rates after transfer of pronuclear microinjected embryos for the production of transgenic Korean black goats. Embryo transfer was carried out in 343 recipient Korean black goats from September 1999 to June 2000. Estrus was induced by the insertion of intravaginal progesterone devices $CIDR^(R)$ for 2 weeks. A single injection of 400 IU equine chorionic gonadotropin was administered at 48h before $CIDR^(R)$ removal to increase the proportion of does cycling and ovulation rate. Good quality embryos were prepared by microinjection of DNA into the pronuclei of fertilized goat oocyte and cultured in vitro. Pronuclear microinjected $1{\sim}8$ cell stage embryos were surgically transferred into the oviducts of the recipient at day 4 or 5 following $CIDR^(R)$ removal, and morula to blastocyst stage embryos were surgically transferred into uterus at day 9. Pregnancy was diagnosed by transrectal ultrasound scanning at $20{\sim}30d$ and 8 weeks following embryo transfer. The pregnancy rate was affected by several factors, such as estrus induction, the number of previous transfer, transfer site, stage of CL (corpus luteum), the number of recipient CL, stage of embryos and the number of transferred embryo. The pregnancy rate was significantly higher in recipients that came into estrus naturally than recipients that induced to come into estrus with $CIDR^(R)$(59.1% vs. 36.8%; P<0.05). The pregnancy rate was higher when the embryos were transferred into the left oviduct than transferred into the right oviduct (42.9% vs. 35.3%; P<0.05). The pregnancy rate of recipients with $CH_1$ (early) stage corpus hemorrhagicum in ovary was hi틴or than recipient with $CH_3$ (late) stage hemorrhagicum (47.5% vs. 17.9%; P<0.01). Higher pregnancy rates were obtained by transfer of 1-cell stage embryos into oviduct while late blastocysts (51.6% vs. 66.7%; P<0.01) into uterus. The pregnancy rates when 3 embryos were transferred to recipients were significantly higher than when 2 embryos we.e transferred (47.6% vs. 27.0%; P<0.05). Although there were no significant difference among the group, adhesion of reproductive organs, uterine size, ovulation rate of recipients, presence of large follicle and difficulty of transfer affected pregnancy rate of recipient. Higher pregnancy rates were obtained in the recipients with $8{\sim}15m$ diameter uterine horn as compared to the recipients with <5m diameter or >20mm diameter uterine hem (38.9%, 20% vs. 18.2%), in the recipients with large follicle in the ovulated ovary ipsilaterally (53.6% vs. 37.1%) and in the transfer which was carried out easily (39.2% vs. 27.8%, 0%). In conclusion, the high rate of pregnancy was achieved following transfer of pronuclear microinjected embryos when three or four 1-cell stage embryos were transferred into oviduct with $CH_1$ stage corpus hemorrhagicum in the ovary of recipient which came into estrus naturally.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2005.10a
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• pp.96-96
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• 2005

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2005.10a
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• pp.97-97
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• 2005

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2005.10a
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• pp.104-104
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• 2005

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2005.10a
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• pp.116-116
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• 2005

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.143-143
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• 2003

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.126-126
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• 2003