• Title/Summary/Keyword: 수소이온 농도

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Geochemistry and Origin of $CO_2$-rich Groundwater from Sedimentary Rocks of Kyungsang System (경상계 퇴적암에서 산출되는 탄산지하수의 지화학적 특성과 생성기원)

  • 정찬호;이진국
    • The Journal of Engineering Geology
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    • v.10 no.1
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    • pp.51-62
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    • 2000
  • The $CO_2$-richrich water pumps or springs out at two sites (Sinchon and Kohran) consisting of Cretaceous sedimentary rocks in Kyungpook area. The water has been long known as its soda pop-liketaste and therapeutic effect against calcium deficit, stomach and skin troubles, etc. The water arecharacterized by a high $CO_2$ concentration $(P_{CO2}=0.29~l.01 atm)$ and electrical conductance (1,093~2,810$\mu$S/cm). The $CO_2$-rich water belongs to Ca(Na)-$HCO_3$ type in chemical classification. The contents of Ca, Mg, Na, HCO$_3$ and Fe of $CO_2$-rich water show much higher values than those of general groundwater Environmental isotopic data $(^2H/^1H, ^{18}O/^{16}O and ^3H/^1H)$ indicate that the water is ofmeteoric origin recharged after 1950s. The $CO_2$ in the springs seems to be originated from deep-seatedsource related to acidic porphyry and granite nearby sedimentary rocks. Carbonate minerals and albiteare likely to be the major source minerals of the dissoved inorganic constituents in the $CO_2$-rich water.The equilibrium state between major minerals and $CO_2$-rich water was calculated by a thermodynamicprogram.

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Purification and Characterization of $\beta$-Xylosidase B of Bacillus stearothemophilus No.236 Produced by Recombinant Escherichia coli. (재조합 균주 Escherichia coli가 생산하는 Bacillus stearothermophilus No.236 $\beta$-Xylosidase B의 정제 및 특성)

  • 장욱진;조쌍구;최용진
    • Microbiology and Biotechnology Letters
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    • v.26 no.4
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    • pp.297-302
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    • 1998
  • $\beta$-Xylosidase B was produced by Escherichia coli HB101/pKMG12 carrying the xylB gene of Bacillus stearothermophilus No.236 on its recombinant plasmid. The $\beta$-xylosidase B produced was purified by ammonium sulfate fractionation, DEAE-Sepharose CL-6B, Sephacryl S-200 and Superdex 200 HR gel filtration. The purified enzyme showed the highest activity at pH 6.5 and 5$0^{\circ}C$. But, the enzyme was observed to be very sensitive to the pH and temperature of the reaction mixture. The enzyme was activated about 35% of its original activity in the presence of 1 mM of $Mn^{2+}$ but it was completely inhibited by $Ag^{+}$, $Cu^{2+}$and $Hg^{2+}$ions. In contrast with the $\beta$-xylosidase A, the B enzyme was found to have $\alpha$-arabinofuranosidase activity though the activity was fairly low compared with the $\alpha$-arabinofuranosidase produced from the arfI gene of the same Bacillus stearothermophilus. Therefore, $\beta$-xylosidase B is considered to be more suitable than $\beta$-xylosidase A at least for the biodegradation of arabinoxylan. The $K_{m}$ and V$_{max}$ values of the $\beta$-xylosidase B for o-nitrophenyl-$\alpha$-D-xylopyranoside were 6.43 mM and 1.45 $\mu$mole/min, respectively. Molecular mass of the enzyme was determind to be about 54 kDa by SDS-PAGE and 160 kDa by Superdex 200HR gel filtration, indicating that the functional $\beta$-xylosidase B was composed of three identical subunits.s.

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Self-purification Mechanisms in Natural Environments of Korea: I. A Preliminary Study on the Behavior of Organic/Inorganic Elements in Tidal Flats and Rice Fields (자연 정화작용 연구: I. 갯벌과 농지 상층수중 유 ${\cdot}$ 무기 원소의 거동에 관한 예비 연구)

  • Choi, Kang-Won;Cho, Yeong-Gil;Choi, Man-Sik;Lee, Bok-Ja;Hyun, Jung-Ho;Kang, Jeong-Won;Jung, Hoi-Soo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.5 no.3
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    • pp.195-207
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    • 2000
  • Organic and inorganic characteristics including bacterial cell number, enzyme activity, nutrients, and heavy metals have been monitored in twelve acrylic experimental tanks for two weeks to estimate and compare self-purification capacities in two Korean wet-land environments, tidal flat and rice field, which are possibly different with the environments in other countries because of their own climatic conditions. FW tanks, filled with rice field soils and fresh water, consist of FW1&2 (with paddy), FW3&4 (without paddy), and FW5&6 (newly reclaimed, without paddy). SW tanks, filled with tidal flat sediments and salt water, are SW1&2 (with anoxic silty mud), SW3&4 (anoxic mud), and SW5&6 (suboxic mud). Contaminated solution, which is formulated with the salts of Cu, Cd, As, Cr, Pb, Hg, and glucose+glutamic acid, was spiked into the supernatent waters in the tanks. Nitrate concentrations in supernatent waters as well as bacterial cell numbers and enzyme activities of soils in the FW tanks (except FW5&6) are clearly higher than those in the SW tanks. Phosphate concentrations in the SW1 tank increase highly with time compared to those in the other SW tanks. Removal rates of Cu, Cd, and As in supematent waters of the FW5&6 tanks are most slow in the FW tanks, while the rates in SW1&2 are most fast in the SW tanks. The rate for Pb in the SW1&2 tanks is most fast in the SW tanks, and the rate for Hg in the FW5&6 tanks is most slow in the FW tanks. Cr concentrations decrease generally with time in the FW tanks. In the SW tanks, however, the Cr concentrations decrease rapidly at first, then increase, and then remain nearly constant. These results imply that labile organic materials are depleted in the FW5&6 tanks compared to the FW1&2 and FW3&4 tanks. Removal of Cu, Cd, As from the supernatent waters as well as slow removal rates of the elements (including Hg) are likely due to the combining of the elements with organic ligands on the suspended particles and subsequent removal to the bottom sediments. Fast removal rates of the metal ions (Cu, Cd, As) and rapid increase of phosphate concentrations in the SW1&2 tanks are possibly due to the relatively porous anoxic sediments in the SW1&2 tanks compared to those in the SW3&4 tanks, efficient supply of phosphate and hydrogen sulfide ions in pore wates to the upper water body, complexing of the metal ions with the sulfide ions, and subsequent removal to the bottom sediments. Organic materials on the particles and sulfide ions from the pore waters are the major factors constraining the behaviors of organic/inorganic elements in the supernatent waters of the experimental tanks. This study needs more consideration on more diverse organic and inorganic elements and experimental conditions such as tidal action, temperature variation, activities of benthic animals, etc.

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Active Transport Characteristics of Anions through a Cell Membrane Model which Irradiated by γ-ray (감마선이 조사된 세포막모델을 통한 음이온의 능동 전달 특성)

  • Ko, In-Ho;Yeo, Jin-Dong
    • Journal of the Korean Society of Radiology
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    • v.9 no.4
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    • pp.187-195
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    • 2015
  • The active transport characteristics of anions of cell membrane model which irradiated by $^{60}Co\;{\gamma}-ray$ was investigated. The cell membrane model used in this experiment was a sulfonated copolymerized membrane of poly(1-methyl-4-vinylpyridiniumiodide-co-divinylbenzene : MeVP-DVBI). First, the initial flux of $OH^-$ and $Cl^-$, $Na^+$ of membrane which was not irradiated was decreased with increase of thickness of membrane $80-200{\mu}m$, increased with increase of NaOH concentration 0-0.5mol/L and MeVP-DVBI concentration 20-80% was increased with initial flux of $OH^-$ and $Cl^-$, decreased with initial flux of $Na^+$. Second, the initial flux of membrane which was irradiated was less than that. And the driving force of pH of irradiated membrane was significantly increased more than membrane which was not irradiated. The initial flux of the $OH^-$ ion was decreased with increase of $H^+$ ion concentration. As selective transport of $OH^-$ and $Cl^-$ of cell membrane model were abnormal, cell damages were appeared at cell.

Effect of Evaluation Conditions on Electrochemical Accelerated Degradation of PEMFC Polymer Membrane (PEMFC 고분자 막의 전기화학적 가속 열화에 미치는 평가조건들의 영향)

  • Sohyeong Oh;Donggeun Yoo;Suk Joo Bae;Sun Geu Chae;Kwonpil Park
    • Korean Chemical Engineering Research
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    • v.61 no.3
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    • pp.356-361
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    • 2023
  • In order to improve the durability of the proton exchange membrane fuel cell (PEMFC), it is important to accurately evaluate the durability of the polymer membrane in a short time. The test conditions for chemically accelerated durability evaluation of membranes are high voltage, high temperature, low humidity, and high gas pressure. It can be said that the protocol is developed by changing these conditions. However, the relative influence of each test condition on the degradation of the membrane has not been studied. In chemical accelerated degradation experiment of the membrane, the influence of 4 factors (conditions) was examined through the factor experiment method. The degree of degradation of the membrane after accelerated degradation was determined by measuring the hydrogen permeability and effluent fluoride ion concentration, and it was possible to determine the degradation order of the polymer membrane under 8 conditions by the difference in fluoride ion concentration. It was shown that the influence of the membrane degradation factor was in the order of voltage > temperature > oxygen pressure > humidity. It was confirmed that the degradation of the electrode catalyst had an effect on the chemical degradation of the membrane.

Performance of an Intermittent Aerated Pilot-scale Reactor Vessel for Commercial Composting (상업용 퇴비화를 위한 간헐통기식 파이로트 규모 반응조의 성능)

  • Hong, Ji-Hyung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.6 no.2
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    • pp.31-44
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    • 1998
  • Mixtures of hog manure slurry and sawdust were composted by an intermittent aeration method to verify the performance evaluation of pilot-scale reactor vessels during composting high rate (decomposition) process. Instrumentation was designed to measure temperatures in compost, oxygen and carbon dioxide concentration, air flow rates, and ammonia gas emitted. It was found that ammonia concentration during composting high rate decreased more quickly to the allowable range of 34-40 ppm after 14days at near the optimal levels (II) than in the case of lower levels (I). The influence of the optimal levels (II) such as moisture content (55-65%), C/N ratio (20-40), pH (7-8) and temperature in compost (<$60^{\circ}C$) on the reduction of ammonia gas was considerable for commercial composting.

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Real-time Pesticide Assay on Live Tissue Using Electrochemical Graphite Pencil Electrode (살아있는 세포에서 전기화학적 흑연 연필심 전극을 사용한 살균제의 실시간 분석)

  • Lee, Su-Yeong
    • Journal of the Korean Chemical Society
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    • v.50 no.3
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    • pp.208-215
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    • 2006
  • A simply prepared graphite, pencil-type working electrode was utilized to monitor fenitrothion concentrations, using the cyclic voltammetry (CV) and square-wave (SW) stripping voltammetry methods. The optimum conditions for analysis were sought. A very low detection limit was obtained compared to that obtained when other common voltammetry methods are used. The optimal parameters of the pencil-type electrode were found to be as follows: a pH of 3.7, a frequency of 500 Hz, an SW amplitude of 0.1 V, an increment potential of 0.005 V, an initial potential of -0.9V, and a deposition time of 500 sec. The analytical detection limit was determined to be 6.0 ngL-1 (2.16410-11 molL-1) fenitrothion at SW anodic and CV, and the relative standard deviation at the fenitrothion concentration of SW anodic 10 ugL-1 was 0.30% (n = 15) under the optimum conditions. Analysis was directly conducted through in-vivo real-time assay.

Characterization of Humic Acid in the Chemical Oxidation Technology(I) - Characteristics by Photocatalytic Oxidation Process - (화학적 산화법에 의한 부식산의 분해 처리기술에 관한 연구(I) - 광산화공정을 통한 부식산의 분해특성 분석 -)

  • Kim, Jong Boo;Rhee, Dong Seok
    • Analytical Science and Technology
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    • v.13 no.2
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    • pp.234-240
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    • 2000
  • The efficiency of Photocatalytic Oxidation Process were investigated for the treatment of Aquatic Humic Substances (AHS). In UV-only system, pH 7-9 was the optimum pH range for TOC removal, and alkali range was the optimum pH for absorbance decrease. In UV/$TiO_2$ system, the optimum $TiO_2$ dosage was 50ppm and over 50ppm of $TiO_2$ dosage was not effective for removal of AHS. In UV/$H_2O_2$ system, optimum $H_2O_2$ dosage was 20mM, when over 20mM dosage, removal of TOC (Total Organic Carbon) and absorbance was decreased. Radical scavenger affected on the photo-oxidation of AHS. Removal rate of TOC and absorbance was decreased by addition of carbonate ions and TOC removal was more effected than that of absorbance.

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Studies on Heat Sensitivity of Egg Albumen I. Effects of Heating Time and Temperature, pH and NaCl Concentration on Heat Sensitivity of Egg Albumen (난백 의 열감수성 에 관한 연구 I. 가열온도와 시간, pH 및 NaCl농도가 난백의 열감수성에 미치는 영향)

  • 유익종
    • Korean Journal of Poultry Science
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    • v.15 no.1
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    • pp.39-44
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    • 1988
  • This study was undertaken to find out the effect of heating time and temperature, pH and NaCl concentration on heat sensitivity of egg albumen during heat treatment. Sharp increase of the turbidity and rapid decrease of the foaming power were observed when egg albumen was heated at above $60^{\circ}C$. Egg albumen became opaque when it was heated at $60^{\circ}C$ for above 13 minutes or at $65^{\circ}C$ for above 5 minutes. The turbidity was markedly increased at below pH 7 and the foaming power was largely decreased at around pH 4.0 by the heat treatment ($60^{\circ}C$, 5 min). The foam stability was slightly decreased at alkaline pH range by the heat treatment (60t, 5 min). The addition of NaCl up to 0.3M decreased the turbidity. There was no effect of NaCl addition on the foaming power, but the foam stability was decreased by the addition of NaCl at above 0.2M before and after the heat treatment ($60^{\circ}C$, 5 min).

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Comparison of hypertonic saline treatment in meningitis with cerebral edema (뇌수막염에서 발생한 뇌부종 치료에서 고장성 식염수 비교)

  • Kim, Hyung Su;Kim, Hee Ra
    • Clinical and Experimental Pediatrics
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    • v.49 no.12
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    • pp.1275-1281
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    • 2006
  • Purpose : Cerebral edema in meningitis is a potentially complication. Hypertonic saline of various concentrations are frequently used to treat cerebral edemas in meningitis. We analyzed the safety and efficacy of osmotic therapy in cerebral edema by comparison of various hypertonic saline concentrations and mannitol. Methods : The medical records of 42 patients, who were followed up in the Department of Pediatrics, Busan Medical Center, from Jan. 2002 to Oct. 2005, were analyzed retrospectively. We measured intracranial pressure, mean flow velocity, and various laboratory parameters. Results : In cerebral edema developed in meningitis, intracranial pressure and symptoms were improved in treatment of hypertonic saline and mannitol. Serial bolus infusion of 3 percent hypertonic saline resulted in the best outcome. There was not a statistically considerable difference on the mean values of the intracranial pressure gap. On transcranial doppler, mean flow velocity was increased and pulsatilty index was decreased. Laboratory findings (osmolarity, Na, Cl, pH, lactic acid, Ca) were diffenent during the treatment period as opposed to K, Hb, bicarbonate, base excess. There was not a specific form of hypertonic saline used in meningitis treatment with cerebral edema. Conclusion : The therapy for cerebral edema in meningitis remains largely empirical. Serial bolus infusion of 3 percent hypertonic saline is better than other hypertonic salines. Various concentrations and different infusion methods of hypertonic saline statistically does not influence the result of treatment. More research aimed at improving cerebral edema treatment is needed to identify new, effective forms of treatment.