• Title/Summary/Keyword: 수분 전달

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생쥐 정소에서 Aquaporin9의 발현

  • 강희정;계명찬
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.62-62
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    • 2003
  • Aquaporin (AQP) family protein은 일종의 수분 전달 통로 역할을 하는 단백질로 AQP를 통한 수분의 조절은 삼투압을 통한 물의 이동과 함께 조직내 정상적인 수분의 상성 유지에 필수적이다. 현재까지 11종의 AQP이 신장·뇌·정소·안구 등에서 발현이 확인되었다. AQP9은 물 뿐 아니라 carbamide, polyol, purine, pyrimidine, urea, glycerol 등의 이동에 관여한다. 본 연구에서는 생쥐에서 출생 후 성체에 이르는 동안 정소 내 AQP9의 발현, Leydig cell의 분화에 따른 AQP9의 발현을 조사하였다. 1, 2, 4, 8주령의 정소로부터 semiquantitative RT-PCR 및 real time PCR 법으로 AQP9의 발현을 분석한 결과 1주령에서는 발현되지 않았고 2주령에서는 미량이 발현되기 시작하였고, 4주령에서는 성체의 1/2수준으로 발현량이 급격히 증가하였고 성체에서는 다량으로 발현됨이 확인되었다. Semiquantitative RT-PCR 법과 real time PCR법을 비교할 때 주령별 발현 양상은 유사하였으나 4주령과 성체에서는 두 시험법 사이에 양적인 차이가 있었다. 면역조직화학염색 결과 주로 Leydig cell에서 AQP9의 발현이 확인되었다. 성체의 정소 균질액의 Western blot 상에서 분자량 80, 55, 35 및 23 kDa의 항원이 검출되어 dimer, trimer 형태로 존재할 가능성과 당쇄 결합에 의한 단백질의 변형이 있는 것으로 추정된다. 미성숙 개체의 정소에서는 23 form이 확인되는 반면 성체에서는 35 kDa form이 주로 발현되므로 정소에서 발현되는 AQP9의 경우 Post-translation 수준에서 AQP9의 변형이 수반되는 것으로 사료되며 AQP9의 기능과의 연관성은 추후 연구되어야 할 것이다. Leydig cell은 fetal 및 adult type 2종의 세포가 정소발달 과정에 출현, 사멸, 분화하며 이들은 각기 정소발달, 성숙과 정자형성에 필요한 steroidogenesis에 관여한다. 정소 내 AQP9의 발현은 17beta HSD의 발현 양상과 같게 나타나므로 성적 성숙에 따른 정소 내 AQP9의 발현의 증가는 adult type Leydig cell의 분화와 관련된 것으로 추측된다. 성체의 정소로부터 분리한 Leydig cell-enriched culture에 hCG를 처리한 결과 배양체의 AQP9의 발현이 증가하므로 AQP9은 LH 수용체 하위 신호전달과정을 통해 Leydig cell의 steroidogenesis 또는 생성된 steroids의 분비에 요구되는 수분 및 중성용질의 이동에 관여하는 것으로 사료된다.

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FTIR and Moisture Effects on Optical Information Transfer at Interface of Air and Glass (공기-유리 계면에서 광학정보 전달에 미치는 불완전 반사 및 수분 효과)

  • Han, Won Heum;Han, Ji Heum;Kim, Jee Hyen;Jung, Hyung Sik;Lee, Moon Ho
    • Journal of Adhesion and Interface
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    • v.13 no.2
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    • pp.73-84
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    • 2012
  • The transfer mechanism of optical information at the interface of air and glass (the air-glass IF) has been investigated by thoroughly fulfilling the theoretical and experimental analyses regarding the FTIR (Frustrated Total Internal Reflection) and moisture effects on the fingerprint onto a glass cup with water. As for the fingerprint onto a glass cup with water its image was observed to be very vivid, which turned out to be due to the difference between the two light intensities reflected on the air-glass IF and the wet fingerprint ridge by manipulating the optical theories such as Fresnel relation, Snell's law, FTIR, GT (general transmission) and so on. In addition, the experimental inspection for FTIR and moisture effects on the fingerprint image also evidenced the fact that the vivid fingerprint image originated from the moisture effect rather than the FTIR phenomenon.

Comparisons of Thermal-moisture Properties in Combination of 3D spacer and Polyurethane(PU) Foam for Mold Brassiere Cups (몰드 브래지어 컵의 제작을 위한 3D 스페이서 패브릭과 폴리우레탄(PU) 폼 조합에 따른 열·수분 전달 특성 비교)

  • Lee, Hyun Young;Park, Huiju
    • Korean Journal of Human Ecology
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    • v.24 no.2
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    • pp.285-295
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    • 2015
  • To identify optimized thermal properties of mold brassiere cup for improved thermal comfort during summer, we compared the thermal resistance and the water vapor permeability of Polyurethane (PU) foam, 3D spacer fabric and the two combined materials of the PU foam and the 3D spacer fabric. Four experimental mold brassieres were made of the materials for wearing test. Six women in their twenties evaluated the wearing sensation in the hot and humid environment. The changes in microclimate temperature and humidity while wearing test brassiere cups were measured. Results indicate that thermal resistance increased as more PU foam were combined, while the water vapor permeability was higher as the content of the 3D spacer fabric increased at thickness of 18mm and over. However, in the wear test, the PU foam brassiere was the most preferred in all ambient conditions due to its soft, flexible and smooth texture, despite its high thermal resistance and low water vapor permeability. This indicates that the textures of mold foams are more dominant properties than thermal properties for mold foams in determining the wear comfort of mold brassieres.

Moisture Transfer and Velocity of Moisture Transmission by Wood in Steady State (정상상태(定常狀態)에 있어서 목재(木材)의 습기전달(濕氣傳達)과 투습속도(透濕速度))

  • Lee, Weon Hee
    • Current Research on Agriculture and Life Sciences
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    • v.10
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    • pp.41-47
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    • 1992
  • In general, the behavior of moisture transmission is estimated by vapor permeability or vapor transmission resistance, but its values obtained by experiments do not have great adaptability for practical situations because of changes in the experimental conditions. This fact is why only theoretical discussions have advanced. Thus, the fundamental study of the moisture transmission phenomenon has been treated lightly. Here, as the first step toward the basic research of moisture transmission, the amount of moisture transmission and the moisture distribution in specimens were investigated. The experiment was conducted in a steady state, and the moisture distribution was measured by slicing and weighing the specimens. From the examination of the vapor transmission resistance, the phenomenon of moisture transmission was dealt with devide the moisture transfer on the wood surface and moisture diffusion in wood. The following results were obtained. 1) The phenomenon of moisture transmission should be approached by its division into moisture transfer on the wood surface and moisture diffusion in the wood because the positive values of vapor transmission resistance exist in the extrapolation of thickness 0mm. 2) The distribution of moisture in wood can be illustrated by two straight lines intersecting at the point of nine percent moisture content : namely, diffusion coefficients have two constant values at moisture contents below and above nine percent. The shape of the distribution curve of moisture content is similar irrespective of the wood thickness. On the other hand, when the moisture contents on both sides was more than nine percent, the distribution of the moisture content could be illustrated by one straight lines. 3) The amount of moisture movement is determined by the moisture gradient in wood. 4) Coefficients of the moisture transfer depend on the thickness of the specimens.

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Anti-Inflammatory Activities of Hog Millet (Panicum miliaceum L.) in Murine Macrophages through IRAK-4 Signaling (대식세포에서 IRAK-4신호조절을 통한 기장(Panicum miliaceum L.)의 항염증능에 관한 연구)

  • Park, Mi-Young;Kim, Jae-Hyun;Park, Dong-Sik
    • The Korean Journal of Food And Nutrition
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    • v.24 no.2
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    • pp.268-272
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    • 2011
  • 전곡류의 섭취와 만성질환의 유병율은 음의 상관관계가 있는 것으로 알려져 있다. 본 연구에서는 선행 연구 결과, 지방축적억제능이 우수한 소재로 선정된 기장의 항염증능 여부를 검증하고자 하였다. 이를 위해 RAW264.7 세포에 기장열수분획($1\;{\mu}g/m\ell$$10\;{\mu}g/m\ell$)과 lipopolysaccharide(LPS)를 함께 처리한 후, 24시간 배양시켜 염증매개인자들의 분비량 및 mRNA 발현 정도를 측정하였다. 또한 LPS 자극에 대한 첫 번째 신호전달인자로 알려져 있는 interleukin-1 receptor associated kinase-4(IRAK-4)의 단백질 발현 정도를 측정하였다. 본 연구결과, 기장의 열수분획($10\;{\mu}g/m\ell$)은 LPS로 유도된 NO, $PGE_2$, TNF-${\alpha}$, IL-6 및 MCP-1의 생성량 및 mRNA 발현량을 유의적으로 억제하였다(p<0.05). 특히 이들 지표 중 pro-inflammatory cytokine인 TNF-${\alpha}$와 IL-6의 mRNA 발현량이 효과적으로 감소하였다(p<0.01). IRAK-4의 단백질 발현량 또한 유의적으로 감소하여 LPS 자극에 대한 기장열수분획의 항염증능은 toll-like receptor(TLR)를 통한 IRAK-4를 매개로 하는 신호전달체계 조절에 기인하는 것으로 사료된다.

Numerical Investigation of the Effect of IR Heating on Drying Mechanism in a Tumble Dryer (열복사를 적용한 드럼 건조기의 건조 메커니즘 분석 및 성능 예측에 관한 연구)

  • Choi, Chul-Jin;Jang, Jung-Hyun;Kim, Chong-Min;Kim, Man-Young
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.34 no.3
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    • pp.219-228
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    • 2010
  • A two-dimensional mathematical model was developed to predict the temperature and moisture-content profiles of a tumble dryer during infrared drying. The model is based on the movements of liquid water and moisture in the object and on the fluid and heat transfer in the drying air. The model was solved by the finite volume analysis for the fluid, temperature, and radiation intensity fields. After deriving the governing equations and developing the two-dimensional tumble dryer models, numerical investigations were carried out to examine the effects of various parameters such as the heater temperature and the heating patterns on the drying mechanism of the tumble dryer. All the results show that the drying time can be reduced by using the IR heater.

A Numerical study on the Moisture Transport of Concrete Tunnel Linings with the Sprayable Waterproofing Membrane (뿜칠 방수 멤브레인이 시공된 터널 라이닝의 수분이동에 관한 수치해석 연구)

  • Lee, Chulho;Choi, Soon-Wook;Kang, Tae-Ho;Chang, Soo-Ho
    • Tunnel and Underground Space
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    • v.26 no.3
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    • pp.212-219
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    • 2016
  • The sprayable waterproofing membrane is installed between shotcrete to provide crack bridging and hence prevent flow of liquid water as a waterproofing system. Because of its material characteristics, the sprayable membrane can be constructed at more complex structure than sheet membrane. The main component of the sprayable waterproofing membrane is a polymer-based material, therefore, moisture can migrate through sprayable waterproofing membrane materials by capillary and vapor diffusion mechanisms. The moisture transport mechanisms can have an influence on the degree of saturation and may influence the pore pressure and risk of freeze-thaw damage on concrete linings and membrane. In this study, long-term hygrothermal behavior was simulated with considering moisture transport and long-term effects on saturation of tunnel linings. From the simulation, due to water absorption and vapor transport properties of sprayable membrane, change of relative humidity and water content in tunnel lining can be evaluated.

Growth and Physiological Responses of Pinus strobus to CaCl2 (염화칼슘에 의한 스트로브잣나무의 생장 및 생리반응)

  • Je, Sun-Mi;Kim, Sun-Hee
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.3
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    • pp.1-8
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    • 2017
  • The present study aimed to investigate the effect of calcium chloride($CaCl_2$) on the growth and physiological responses of Pinus strobus and the variables that are sensitive to $CaCl_2$. Thus, changes in the visible damage, growth of root collar diameter, plant water content, chlorophyll content and composition, maximum PS II photochemical efficiency, and electron transport rate of P. strobus was analyzed in relation to treatment witih $CaCl_2$. A $CaCl_2$ solution(0.5, 1.0 and 3.0%) was applied in the root zone before leaf unfolding. Leaf browning, defoliation, and drying were observed with $CaCl_2$ application and this pattern was aggravated as the $CaCl_2$ concentration increased and the treatment period became longer. The decrease of growth in root collar diameter and height and leaf water content were observed at $CaCl_2$ 1.0% and 3.0%. The total chlorophyll content indicated that photopigment, PS II photochemical efficiency and electron transport rate significantly decreased at $CaCl_2$ 3.0%. In conclusion, $CaCl_2$ affected leaf water content and led to a decrease of capability in light harvesting and photochemical responses. Also, as a result of the correlation between calcium chloride concentration and growth and physiological response parameters, it was found that the leaf moisture content and the ratio of chlorophyll a and b reflect the damage level of calcium chloride sensitively because their coefficient of determinations were relatively high.

Development of an End-Effector for Cucumber Robotic Harvester (오이 로봇 수확용 엔드이펙터 개발)

  • 민병로;문정환;이대원
    • Journal of Bio-Environment Control
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    • v.12 no.2
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    • pp.63-67
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    • 2003
  • Cucumber fruits requires a lot of labor to harvest in time in Korea, since the fruits are cut and grabbed by hand. In this study, we developed an end-effector for robotic harvester of cucumber fruits. Its development involved the integration of an end-effector system with a PC compatible, DC motors, and a motor controller board. Software, written in Pic-basic, combined the functions of motor control with various circumstances. Cucumber's properties were measured and analyzed for precision of the end-effector. The results were similar to those of other vegetables. Properties including hardness of cucumber fruits were used as basic data for development of a harvester.