• 한국동물학회지
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• 제35권1호
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• pp.80-86
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• 1992

The nucleotide sequences of 185 rRNAs of the five Korean decapods were partially determined by the direct sequencing method using the reverse transcriptase. ne average GC content of five species was 51.1% which is higher than that of yeast(45.0%) and lower than those of frog (53.0%) and rat (55.6%). This result follows the general patterns of the GC content in the nucleotides of the nucleic acid shown among the various phylogenetic groups. The average ratio of transrional/transversional nucleotide substitution of pairwise comparison among six species (including Anemia salina) was 1.200 $\pm$ 0.310 when whole region alas examined. However, the ratio showed some differences when the conservative regions and variable regions frere separatelv examined. The molecular phylogenies of the five species were constructed by using two different tree making methods. In general the results support the previously reported molecular phylogeny of the decapod crustaceans. However, our results indicate thats in the analysis of the sequence dat3, the UPGMA clustering method of the distance matrix method should be carefully employed after considering the rate of nucneotide substitution in the different regions of the molecule.

• Journal of Pharmaceutical Investigation
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• 제28권1호
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• pp.43-50
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• 1998

Niosomes are vesicles formed from synthetic non-ionic surfactants, offering an alternative to chemically unstable and expensive liposomes as a drug carrier. Non-ionic surfactant and cholesterol mixture film leads to the formation of vesicular system by hydration with sonication method. The formation of niosome was ascertained by negative staining of TEM. The entrapment efficiency of niosomal suspension was gradually increased with increasing the ratio of cholesterol to surfactant. It was found that the niosome with 6 : 4 (polyoxyethylene 2-cetyl ether: cholesterol) ratio was more stable than those with other ratios. The topical application of acyclovir(ACV) in the treatment of herpes simplex virus type 1(HSV-1) skin disease has a long history. There are an increasing number of reports, however, in which topical ACV therapy is not as effective as oral administration. Lack of efficacy with topical ACV has been hypothesized to reflect the inadequate delivery of drug to the skin. We investigated the permeation of niosome containing $[^{3}H]ACV$ in hairless mouse skin using Franz diffusion cell model. Permeation coefficient(P) of aqueous ACV was $6.7{\times}10^{-4}\;(cm/hr)$ and that of ACV in niosome was $23.4{\times}10^{-4}\;(cm/hr)$, suggesting about 3.5 times increase in the transdermal permeation.

• Journal of Pharmaceutical Investigation
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• 제26권4호
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• pp.291-297
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• 1996

Liposomes of $pSV-{\beta}-Galactosidase$ vector plasmid DNA with various lipid composition were prepared by the thin-film method. Size distribution, shape and the efficiency of plasmid DNA encapsulation were investigated. Effect of sonication time on the plasmid DNA entrapment in liposomes and stability at $4^{\circ}C$ were also examined. Sizes of neutral liposomes were about 100-200 nm and above $1\;{mu}m$, and those of cationic liposomes were about 400-600 nm and above $1\;{mu}m$. Shapes of liposomes entrapped plasmid DNA were spherical. Proper sonication time for better entrapment was below 15 minutes and stability at $4^{\circ}C$ was decreased rapidly after 1 day. Plasmid DNA entrapments of complex liposomes of various lipids were higher than those of liposomes made from one sort of lipid. Plasmid DNA entrapments of cationic liposomes were higher than those of neutral liposomes.

• Journal of Pharmaceutical Investigation
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• 제34권4호
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• pp.305-310
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• 2004

This study was to prepare the anionic liposomes which were to release anticancer drug (doxorubicin) at the hyperthermia temperature $({\sim}42^{\circ}C)$ and to stabilize in bovine serum solution at $37^{\circ}C$. The vesicle size and zeta potential of liposomes in Tris-HCl buffered solution (pH 7.4) were measured by an electrophoretic light scattering spectrophotometer. To estimate the stability of liposomes, liposome size was measured in bovine serum solution at $37^{\circ}C$ for 72 h. The release of doxorubicin from liposome was determined by measuring the fluorescence intensity using fluorescence spectrophotometry with temperature and time. The size of liposomes was from 120 to 160 nm and zeta potential was from $-33.3{\pm}2.4$ to $-75.6{\pm}6.9\;mV$. Anionic liposome was stabilized in bovine serum solution at $37^{\circ}C$ within 72 h. Additionally, the release transition temperature of doxorubicin from liposomes was increased by increasing mole % of anionic phospholipid.

• 미생물학회지
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• 제54권3호
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• pp.280-282
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• 2018

Gammaproteobacteria에 속하는 Pseudoalteromonas sp. meg-B1을 제주도 해양 퇴적물로부터 분리하였다. 본 연구에서는 대략 4.15 Mb의 크기와 41.2%의 평균 G + C 함량을 가진 meg-B1 균주의 완전한 유전체를 보고한다. 유전체는 3,606개의 코딩 서열, 9개의 리보솜 RNA 및 94개의 전사 RNA 유전자가 존재하며, 한 개의 완전한 프로파지 영역이 발견되었다. 본 유전체는 해양환경에서 생존하기 위한 삼투화합성 용질합성과 관련된 유전자(예, choline dehydrogenase)들이 확인되었다.

• 미생물학회지
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• 제53권4호
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• pp.326-328
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• 2017

Betaproteobacteria에 속하는 Herbaspirillum sp. meg3을 제주도 토양으로부터 분리하였다. 본 연구에서는 대략 5.47 Mb의 크기와 57.1%의 평균 G + C 함량을 가진 meg3 균주의 완전한 유전체를 보고한다. 유전체는 4,816개의 코딩 서열, 9개의 리보솜 RNA 및 51개의 전사 RNA 유전자가 존재하며, 두 개의 불완전한 프로파지 영역이 발견되었다. 또한 유전체 분석 결과는 meg3 균주가 방향족 화합물에 대한 분해능을 가지고 있음을 제시하고 있다.

• 미생물학회지
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• 제55권1호
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• pp.61-63
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• 2019

Firmicutes에 속하는 Streptococcus sp. strain NM을 두경부암 환자로부터 분리하였다. 본 연구에서는 약1.90 Mb의 크기와 39.3%의 평균 G+C 함량을 가진 NM 균주의 비완전한 유전체를 보고한다. 유전체는 1,845개의 코딩서열, 12개의 리보솜 RNA 및 58개의 전사 RNA유전자를 포함하였다. 본 유전체로 부터, 항생제내성, 용혈 및 방어시스템과 관련된 유전자들이 확인되었다.

• Journal of Korean Biological Nursing Science
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• 제21권1호
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• pp.37-45
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• 2019

Purpose: This descriptive research was on hand dermatitis in clinical nurses. Nurses often have a high rate of hand dermatitis with several factors associated with the dermatitis. The factors analyzed in this study included; general characteristics, work-related factors, hand washing, and allergies relate to hand dermatitis. Methods: Data were collected from April to May 2016. The subjects were nurses who had worked 12 months or more in a University Hospital. They voluntarily gave their consent for the research. The Symptombased questionnaires were sent to 220 nurses and 199 responses were returned. Collected data were analyzed using frequency, percentage, mean, standard deviation, ${\chi}^2-test$, and logistic regression analysis using SPSS 23.0. Results: Prevalence of dermatitis in nurses was 48.2%. Nurses who had worked for over three years were twice more likely to suffer hand dermatitis than nurses who had worked for 3 years or less (adjusted odds ratio [adj. OR]= 1.97, p= .036). Nurses who used alcohol sponge swabs more than 20 times per shift were 2.5 times more likely to suffer hand dermatitis as compared to those who used them less times (adj. OR = 2.51, p= .023). Conclusion: Results revealed that about a half of the clinical nurses suffered from hand dermatitis, hence it is crucial to find appropriate interventions for dermatitis prevention. This concern needs to be addressed especially in nurses who have more work experience and use more alcohol sponge swabs.

• 미생물학회지
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• 제54권4호
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• pp.442-444
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• 2018

저자들은 최근 급성골수성백혈병 환자로부터 다제내성대장균 균주 KBN10P04869를 분리했다. 균주는 4,457개의 단백질 코딩 유전자, 88개의 운반 RNA, 22개의 리보솜 RNA를 포함하는 5,104,264 염기쌍으로 구성되고, $^{bla}CMY-2$, $^{bla}TEM-1$, $^{bla}CTX-M-15$, $^{bla}NDM-5$, $^{bla}OXA-18$를 포함한 다제내성유전자를 가지고 있다. 저자들은 이 균주의 총유전체를 보고하는 바이다.

• 보건의료산업학회지
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• 제12권3호
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• pp.157-168
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• 2018

Objectives: The purpose of this study was to explore and describe the influencing factors in preventive behavior of tuberculosis among elementary school teachers. Methods: Participants were 151 teachers working at 3 elementary schools in Kimhae City, 3 in Changwon City and 2 in Miryang City. Data were collected with structured questionnaires from May. 8. to Jun. 1. in 2018. Data were analyzed by SPSS/WIN 21.0 program for descriptive statistics, t-test, ANOVA, Pearson's correlation coefficient and stepwise multiple regression. Results: The preventive behavior of tuberculosis among elementary school teachers were significantly positive correlated with knowledge(r=.275, p=.001), attitude(r=.493, p<.001) and self-efficacy(r=.280, p=.001). Moreover, The significant factors of the preventive behavior of tuberculosis were attitude(${\beta}=.341$, p<.001), gender(${\beta}=.407$, p<.001), smoking status(${\beta}=.210$, p=.004), self-efficacy(${\beta}=.187$, p=.005) and knowledge(${\beta}=.145$, p=.026). Conclusions: In this study, in order to promote the preventive behavior of tuberculosis among elementary school teachers, it is necessary to find ways to increase knowledge, attitude, and self-efficacy of tuberculosis, which are significant influencing factors. In addition it would be possible to develop more effective and efficient nursing intervention program if the individual characteristics of the subjects such as gender and smoking status are considered.