• Clinical and Experimental Reproductive Medicine
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• v.33 no.2
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• pp.115-123
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• 2006

Objective: To investigate new signal transduction cascade through integrin, FAK and ERK in the suppressed cell proliferation by GnRH-I and -II. Method: Human endometrial cancer cells (HEC1A) were cultured under the following condition: DMEM/F12 (10% FBS). GnRH-I and -II were treated time (0, 5, 10, 15, 20, 30 min; 100 nM) and dose (10 nM or 100 nM; 20 min) dependent manner according to experimental purposes. Cell proliferation was measured using [$^3H$] thymidine incorporation assay. Immunoblotting was utilized to detect proteins. Results: GnRH-I and -II inhibited proliferation of HEC1A cells and induced expression of integrin ${\beta}3$. Phosphorylation of FAK and ERK were induced by GnRH-I and -II. Conclusion: GnRH inhibited cell proliferation via the expression of integrin and FAK, ERK phosphorylation.

• Journal of the Korean Wood Science and Technology
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• v.43 no.4
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• pp.411-420
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• 2015

Despite of the same species there is a difference in the tree shape and its wood property due to quantitatiVe and qualitatiVe differences in wood cells by the intra-annual cambial actiVity. The purpose of this study was to proVide the fundamental database of intra-annual cambial seasonal actiVity for red pine in Sokwang-ri and Anmyondo for the determination of their wood qualities. As the results of intra-annual cambial actiVity, the initiation of cambial actiVity of red pine in the plots with Age Class V in Sokwang-ri was in the middle of April and it was at the beginning of April in all plots in Anmyondo. Only the plots with Age Class IX in Sokwang-ri showed relatiVely late initiation of cambial actiVity as between middle of May and middle of June. Except one plot with Age Class IX in Sokwang-ri all plots in both sites showed the same cessation of cambial actiVity as between middle and end of October. According to intra-annual cambial actiVity, the duration of cambial actiVity in Sokwang-ri is shorter than that of Anmyondo. On the other hand, the number of cell diVision of cambial actiVity in Sokwang-ri was more than those of Anmyondo. We could establish the database for intra-annual cambial actiVity for Sokwang-ri and Anmyondo to estimate their wood quality.

• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.323-327
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• 1995

This experiment was carried out to observe the origin and developmental pattern of somatic embryos of Oenanthe javanica ($B^{L}.$) DC. The experiment included observation of embryogenic cells and their development stages by light microscope, transmission electron microscope and scanning electron microscope. The embryogenic cells, which were smaller than non-embryogenic cells in size with expanded nucleus and dense cytoplasm. When stained with hematoxylin, the embryogenic cells were readily distinguished from the non-embryogenic cells of which cell walls were stained with safranin. It was observed at somatic embryos developed from single cells on the epidermis of developing embryos or in the surface or inside of embryogenic clumps by segmentation pattern. Observation with a transmission electron microscope revealed that the embryogenic cells had dense cytoplasm expanded nucleus, small vacuoles, large amyloplasts containing starch grains, and abundant organelles including lipid bodies. Under a scanning electron microscope, embryogenic callus was shown to consist of very smaller cells than non-embryogenic cells in an orderly arrangement and covered with a net-like structure, while the non-embryogenic callus consisted of large cells, irregular in size and arrangement, and covered with a gelatin-like material.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.2
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• pp.145-152
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• 2016

Epidermis is continuously regenerated by keratinocyte stem cells (KSCs) residing in basement membrane, which is critical to the survival of an organism. KSCs are believed to persist during the whole lifetime and generate an enormous number of keratinocytes, required for the maintenance of epidermis, through transit amplifying cells dividing definite times until they become differentiated. In this report, we have developed a phenolic compound, paeonol, purified from Moutan Cortex, as a KSC proliferation activator, by screening about 350 herbal compounds. The cell proliferation activation by paeonol is specific for KSC not for keratinocyte, and no significant difference in the expression of p63 protein, a KSC marker, in KSCs treated with paeonol was observed in FACS analysis with anti-p63 antibody. In the colony forming assay, paeonol-treated KSC showed improved colony forming activity more than 1.3 fold. In addition, the result of PCR array shows that the activity of paeonol is through several signal pathways involving stem cell functions. These results suggest that paeonol could enhance KSC proliferation activity without reduction in stemness and could be applied to cosmetics as a KSC activating ingredient.

• The Journal of the Korea Contents Association
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• v.11 no.5
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• pp.32-40
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• 2011

The tracking and analysis of cell activities in time-lapse sequences plays an important role in understanding complex biological processes such as the spread of the tumor, an invasion of the virus, the wound recovery and the cell division. For automatic tracking of cells, the tasks such as the cell detection at each frame, the investigation of the correspondence between cells in previous and current frames, the identification of the cell division and the recognition of new cells must be performed. This paper proposes an automatic cell tracking algorithm. In the first frame, the marker of each cell is extracted using the feature vector obtained by the analysis of cellular regions, and then the watershed algorithm is applied using the extracted markers to produce the cell segmentation. In subsequent frames, the segmentation results of the previous frame are incorporated in the segmentation process for the current frame. A combined criterion of geometric and intensity property of each cell region is used for the proper association between previous and current cells to obtain correct cell tracking. Simulation results show that the proposed method improves the tracking performance compared to the tracking method in Cellprofiler (the software package for automatic analysis of bioimages).

• Journal of Chest Surgery
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• v.34 no.1
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• pp.97-100
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• 2001

흉막의 고립성 섬유성 종양은 중피하에 존재하는 미분화 중배엽성 기원의 섬유 세포에서 기원하는 종양으로 알려졌다. 이 종양은 대부분 양성으로 보고되고 있으나 병리학적으로 높은 세포 밀도, 유사분열의 수가 많고(10개의 고배율 시야에서 4개 이상), 핵의 다양성, 출혈, 괴사등이 있으며 악성의 판단기준으로 보고된다. 환자는 62세 여자로 호흡곤란 및 기침을 주소로 본원에 입원하였으며 단순 흉부 방사선 및 전산화 단층 촬영상 우측 흉강내에 거대한 종양소견이 보였다. 이 종양은 23$\times$18$\times$12 cm, 2 kg의 크기와 무게를 가졌으며 우측폐 하엽과 중엽은 압박되어있었으나 종양 절개 후 재 팽창됨을 확인하였다. 또한 횡경막과 심하게 유착되어 박리도중 손상이 동반되어 봉합술이 필요하였다. 병리학적으로 종괴는 세포밀도가 높았고 방추형세포의 다발로 이루어져 있었으며 유사분열의 수가 많이(27 mitosis/10HPF)보였다. 면역 조직 화학 검사상 vimentin과 CD34에 양성 반응을 보였다. 이에 악성 고립성 섬유성 종양으로 확진되었다. 본 교실에서는 흉막에 발생한 악성 고립성 섬유성 종양을 치험하였기에 문헌 고찰과 함께 보고한다.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.271-275
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• 2007

In the chloroplast transformation process, a chloroplast containing transformed chloroplast genome copies should be selected over wild-type chloroplasts on selection medium. It is more effective for a cell to become homoplasmic if the cell contains smaller number of chloroplasts. Therefore, to reduce the number of chloroplasts in mesophyll cells in tobacco, we overexpressed FtsZ to generate transgenic plants, of which mesophyll cell contained a few enlarged chloroplasts contrast to a wild-type mesophyll cell containing approximately 100 chloroplasts. It was demonstrated that transgenic leaf tissues comprising cells with a few enlarged chloroplasts gave rise to approximately 40% higher frequency of chloroplast-transformed adventitious shoots.

• Journal of Embryo Transfer
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• v.18 no.3
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• pp.263-267
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• 2003

This study was carried out to test the efficacy of pharmacological inhibitors of the cell cycle transition in keeping bovine oocytes at the germinal vesicle(GV) stage and the reversibility of this inhibition. Bovine oocytes were incubated for 22∼24 hrs in the presence of various inhibitors : cycloheximide (2$\mu\textrm{g}$/$m\ell$), 6-DMAP (2 mM), and roscovitine (50$\mu$M). Bovine oocytes cultured with any of the inhibitors were significantly blocked at the GV stage. Reversibility of pharmacological inhibitors was assessed by culturing oocytes an additional 22∼24 hours in inhibitor-free medium. Examination of oocytes revealed that the inhibitory effect was fully reversible and effect of resuming meiotic progression on nuclear maturation varied according to the various inhibitors. This study suggests that cycloheximide, 6-DMAP and roscovitine can be applied to control meiotic arrest and resumption in maturation culture of bovine oocytes in vitro. More investigations are needed to better understand how the cell cycle of oocyte is blocked without problems to future developmental competence.

• Journal of the Korean Wood Science and Technology
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• v.33 no.4 s.132
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• pp.1-8
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• 2005

The initiation and cessation of cambial activities were investigated to find out the growth characteristics of the major species growing in Chuncheon, Korea. The division of cambial zone in Pinus densiflora and Pinus koraiensis began from the middle of March through the end of March. The activity of cambial zone in P. densiflora and P. koraiensis was vigorous from the end of May through the middle of July. During this period the number of cells increased rapidly in radial direction. However, the number of cells in P. densiflora was gradually increased. The division of cambial zone in ring-porous wood, such as Quercus mongolica and Robinia pseudoacacia began in the middle of March, before the formation of vessel. The division of cambial zone in diffuse-porous wood, such as Cornus controversa and Prunus sargentii, began in early April, and then the formation of vessels began after one to three weeks. It was considered that the cambial zone in softwood was ceased from the beginning of November, whereas cambial activities in hardwood ceased in the middle of October. Consequently, the formation of growth ring was different among the species, even though the trees were grown under the same conditions.

• Journal of Embryo Transfer
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• v.16 no.2
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• pp.91-98
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• 2001

We evaluated the effects of the substrate and inhibitors related to phosphatidylinositol metabolism on in vitro maturation and fertilization of porcine oocytes. Cumulus-oocyte complexes were cultured in mTLP-PVA medium supplemented with or without inositol (250 mM) fur 46h. Subsequently, these oocytes were inseminated with fresh boar semen in mTALP-PVA medium for 6h. At 6h after insemination, oocytes were cultured for further 12 h in TCM-199 supplemented with 10% FBS (fetal bovine serum). The higher percentage of oocytes in inositol-supplemented medium reached metaphase of the second meiotic division compared to those in control (81.4% vs. 67.3%; P<0.()5). following 18 h of insemination, more number of male pronuclei were formed in the oocytes matured in inositol-supplemented medium than in those of control experiment (42.0% vs. 27.3%; P<0.05). When oocytes were cultured in medium with 10mM LiCl (chloride lithium) or 0.5mM dbcAMP (dibutyryl cyclic adenosine monophosphate) to determine the role of inositol on the maturation of oocytes, these two drugs inhibited the meiotic division of oocytes (P<0.05). However, addition of inositol to the culture medium did overcome the inhibitory effect of these drugs on the oocyte maturation. DbcAMP and verapamil supplemented synergistically arrested the meiotic division of oocytes. Addition of verapamil did not inhibit germinal vesicle breakdown, but it severly inhibited the second meiotic division of oocytes. These results suggest that inositol exert its improving effects on maturation, by activating the PI (phosphatidylinositol) cycle and causing beneficial changes in both cytoplasm and membrane of oocytes.