• Title/Summary/Keyword: 세포분류학

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A taxonomic study on section Atratae of Carex L. in Korea (Cyperaceae) (한국산 사초속 감둥사초절의 분류학적 연구)

  • Oh, Yong Cha;Lee, Chang Shook;Ryu, Kyung Jin
    • Korean Journal of Plant Taxonomy
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    • v.31 no.3
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    • pp.223-251
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    • 2001
  • Morphological characters of section Atratae of the genus Carex (Cyperaceae) were reexamined. The epidermal patterns of perigynium, achene and leaf were investigated using by a scanning electron microscope (SEM) and a light microscope (LM). Morphological characters such as length and width of stem, leaf, bract, spike, scale, perigynium and achene, and shape of cross-sectioned stem, and leaf, spike, scale, apex of scale, perigynium, beak of perigynium and achene, and epidermal pattern of perigynium, achene and leaf (shape of fundamental epidermal cell and cell wall, type of silica body, shape of beak epidermal cell and cell wall in perigynium, subsidiary cell shape, size and frequency of stomatal complex of leaf) were useful for the identifications of the observed seven taxa. C. gmelinii, C. hancokiana and C. peiktusani have been confused each other due to similar morphological characters. Howerever these taxa were distinct with respect to shape of scale, apex of scale, perigynium, type of silica body, frequence of stomatal complex, subsidal cell shape, and epidermal patterns of achene and leaf.

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International Comparison of Korean Biology Gifted-Students (한국의 생물 영재 학생에 대한 국제적 수준 비교)

  • Shim, Kew-Cheol;Lee, Hyun-Uk;So, Keum-Hyun;Chang, Nam-Kee
    • Journal of The Korean Association For Science Education
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    • v.18 no.4
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    • pp.485-491
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    • 1998
  • In this study, the achievement of Korean biology gifted-students was compared with that of International Biology Olympiad(IBO) participants to explore international achievement level of biology and to suggest the need of teaching program for biology gifted-students. Korean thirty gifted-students were selected through test by Korean Biology Olympiad Committee. They examined theoretical test two times in January and March. 1998. Theoretical part consisted of eight domains as follows; cell biology. anatomy and physiology of plants, anatomy and physiology of animals, ethology. genetics and evolution, ecology, systematics, and microbiology. As a result, Korean biology gifted-students had lower achievement than IBO participants in eight domains. and especially much lower achievement in ethology and systematics. Though thirty Korean gifted-students were found to had much lower achievement than lBO participants, four higher rankers of them are in prospect of winning bronze medals. Thus, it is necessary to develope an appropriate teaching program for biology gifted-students with theoretical lectures and inquiry activities.

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Application and Prospects of Molecular Imaging (분자영상의 적용분야 및 전망)

  • Choi, Guyrack;Lee, Sangbock
    • Journal of the Korean Society of Radiology
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    • v.8 no.3
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    • pp.123-136
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    • 2014
  • In this paper, we study to classify molecular imaging and applications to predict future. Molecular imaging in vivo at the cellular level and the molecular level changes taking place to be imaged, that is molecular cell biology and imaging technology combined with the development of the new field. Molecular imaging is used fluorescence, bioluminescence, SPECT, PET, MRI, Ultrasound and other imaging technologies. That is applied to monitoring of gene therapy, cell tracking and monitoring of cell therapy, antibody imaging, drug development, molecular interaction picture, the near-infrared fluorescence imaging of cancer using fluorescence, bacteria using tumor-targeting imaging, therapeutic early assessment, prediction and therapy. The future of molecular imaging would be developed through fused interdisciplinary research and mutual cooperation, which molecular cell biology, genetics, chemistry, physics, computer science, biomedical engineering, nuclear medicine, radiology, clinical medicine, etc. The advent of molecular imaging will be possible to early diagnosis and personalized treatment of disease in the future.

Nocardia의 생리화학적 연구 (제1보) Nocardia SP의 미세구조에 관하여

  • 홍순덕;정기택
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1977.10a
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    • pp.196.3-196
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    • 1977
  • Nocardia sp는 공업폐액의 활성요인로 부터 형태학적으로는 일반세균과 방선균과의 중위위치에 속하는 분류학상 특이한 미생물로서 이의 유녹균으로서는 Corynebacterium, Mycobacterium을 들수있으나 이들과 형태적인 구별은 매우 어렵다. 그러므로 이들 유연균들과 비교하기 위하여 전자현미경에 의한 미세구조를 관찰하였던 바 세포내 unit membrane을 가진 막양구조가 복잡한 형태로 질내에 분포되에 있음을 알았다.(중략)

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A Study on the Oogeneis of False Dace (Pseudorasbora parva) (참붕어 (Pseudorasbora parva)의 난자형성과정에 관한 연구)

  • Kim, Dong-Heui;Lee, Kyu-Jae;Kim, Seok;Deung, Young-Kun
    • Applied Microscopy
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    • v.37 no.2
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    • pp.65-72
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    • 2007
  • The oogenesis and ultrastructure of fertilized egg envelope of false dace were investigated by light and electron microscope. The cytoplasm of false dace oogonia was basophilic and many nucleoli were located at inner side of nuclear membrane. In primary oocytes, yolk vesicles were distributed in marginal area only and egg envelope was not formed on egg outside. In secondary oocyte, the egg envelope was formed and yolk vesicles were increased than that of early stage in cytoplasm. The amount of basophilic substance was decreased. In case of matured egg, thickness of egg envelope and site of egg were increased, basophilic substance was distributed in egg envelope around only. The yolk vesicles were changed to yolk mass in accordance with development. The fertilized egg was of ellipsoidal, adhesive type and yellowish, have a single micropyle in the area of the animal pole. The fertilized egg envelope consisted of three layers, an outer adhesive layer, a middle layer consisting of 6 lamellae alternating layers and an inner electron dense layer. An outer surface of the fertilized egg envelope was arranged by adhesive fibrous structures. In conclusion, it is summarized that the oogenesis of false dace were the increase of cell size, the formation and accumulation of yolk, and decrease of basophilic intensity in cytoplasm. These ultrastructural characteristics of fertilized egg envelope from false dace can be utilized in taxonomy of teleost.

Morphology and Histology of the Olfactory Organ in the Korean Endemic Species, Pseudobagrus koreanus (Cypriniformes, Cyprinidae) (한국 고유종 눈동자개 Pseudobagrus koreanus 후각기관의 형태 및 조직학적 특성 연구)

  • Kim, Hyun-Tae;Chae, Dong-Hyun
    • Korean Journal of Ichthyology
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    • v.34 no.3
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    • pp.153-159
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    • 2022
  • The olfactory organ of a Korean endemic species, Pseudobagrus koreanus, was investigated to describe its morphology and histology and analyze correlation between habitat environment and its ecological habits and characters, using a stereo microscope, a light microscope, and a scanning electron microscope. Its external morphology showed tubular anterior nostril at the tip of the snout and slit posterior nostril at the base of the nasal barbel. The olfactory chamber showed the rosette structure consisting of numerous lamellae radial and parallel to the medium raphe. The olfactory lamella has the sensory epithelium with olfactory receptor nuerons, supporting cells, basal cells, lymphatic cells, plasma cells and the non-sensory epithelium with stratified epithelial cells, mucous cells, lymphatic cells. In particular, 1) 41~43 (n=20) lamellae, 2) lymphatic cells and plasma cells, 3) mucous cells including neutral polysaccharid may be olfactory characteristics relevant to a stagnant or a standing habitat environment, nocturnal habit dependent on olfaction, and taxonomic characters compared at least to other catfish species.

A study of Conjunctival Cellular Changes in Dry Eye Patients by Impression Cytology (Impression cytology를 이용한 건성안의 결막 세포변화에 관한 연구)

  • Kim, Jai-Min;Kho, Eun-Gyung;Chae, Soo-Chul;Kim, Soon-Ae
    • Journal of Korean Ophthalmic Optics Society
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    • v.9 no.2
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    • pp.333-343
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    • 2004
  • Impression cytology refers to application of cellulose acetate filter material to the ocular surface to remove the superficial layers of the conjunctival epithelium. The technique is non-invasive, is easy to perform, causes minimal discomfort to the patient, and can be used to follow changes in the conjunctival ocular surface over time. With this method, the morphology of the conjunctival ocular surface can be studied and the degree of squmaous metaplasia assessed. This study was performed to evaluate the conjunctival surface by impression cytology in dry eye patients. A total of 70 students with no contact lens wearing history were recruited. Subjects were required to fill in a McMonnies dry eye symptom questionnaire. The non-invasive tear thinning time(TIT) test of each subject was measured, followed by Schirmer tear test(STI), tear film break-up time(TBUT) tests and Rose-bengal staining were performed as a baseline. Conjunctival epithelial cells from the inferior bulbar conjunctiva were harvested by the impression cytology technique. The specimens collected were labelled and stained with PAS(Periodic Acid Schift)-haematoxylin. The goblet cells and conjunctival epithelial cells were observed under a light microscope of 400x magnification. The specimens were classified according to the Nelson Grading scale which was based on the degree of squamous metaplasia such as changes of goblet cells density, size/form, N:C(nucleus : cytoplasm) ratio. Dry eye patients were observed morphological changes of the epithelial cells, different nuclear alterations, decrease of the goblet cells density. The degree of cytological changes was related to severity of dry eye conditions. When the epithelial cell morphology was graded according to the system described by Nelson, specimens from the control group revealed 91.43% of the eyes to be grade 0 and 8.57% to be grade 1, whereas of the dry eye patients, 20% were grade 0, 42.86% grade 1, 34.29% grade 2 and 2,86% grade 3. Impression cytology represents a non- or minimally invasive biopsy of the ocular surface epithelium with no side effects or contraindications. It has demonstrated to be a useful diagnostic aid for a wide variety of processes involving the ocular surface. This technique is a safe, simple method and may help increase understanding of various ocular surface alterations in dry eye patients.

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The Mexican Axolotl (Ambystoma mexicanum) as An Experimental Material for Studies in Embryology: II. Developmental Genetics

  • George M. Malacinski;Chung, Hae-Moon
    • The Korean Journal of Zoology
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    • v.20 no.3
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    • pp.149-157
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    • 1977
  • At present over 3 dozen mutant genes have been recognized in the Mexican axolotl. These genes, all recessives, are categorized in 5 groups according to the nature of their effects and the developmental stage at which their phenotype is predominately expressed. They are genes affecting the oogenesis (maternal-effect genes), genes affecting the size of the nucleolus, genes affecting the development of specific tissues and organs, genes exert lethal effects on all cells or tissues (autonomous lethals), and genes affecting pigment cells. This report describes briefly the phenotypes and some of the current research applications of those genes.

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The System Of Microarray Data Classification Using Significant Gene Combination Method based on Neural Network. (신경망 기반의 유전자조합을 이용한 마이크로어레이 데이터 분류 시스템)

  • Park, Su-Young;Jung, Chai-Yeoung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.12 no.7
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    • pp.1243-1248
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    • 2008
  • As development in technology of bioinformatics recently mates it possible to operate micro-level experiments, we can observe the expression pattern of total genome through on chip and analyze the interactions of thousands of genes at the same time. In this thesis, we used CDNA microarrays of 3840 genes obtained from neuronal differentiation experiment of cortical stem cells on white mouse with cancer. It analyzed and compared performance of each of the experiment result using existing DT, NB, SVM and multi-perceptron neural network classifier combined the similar scale combination method after constructing class classification model by extracting significant gene list with a similar scale combination method proposed in this paper through normalization. Result classifying in Multi-Perceptron neural network classifier for selected 200 genes using combination of PC(Pearson correlation coefficient) and ED(Euclidean distance coefficient) represented the accuracy of 98.84%, which show that it improve classification performance than case to experiment using other classifier.

The taxonomic consideration of petal and sepal micromorphology in Lycopus L. (Mentheae-Lamiaceae) (쉽사리속(Lycopus속, 박하족-꿀풀과) 화판 및 악편 미세형태의 분류학적 검토)

  • Moon, Hye-Kyoung;Hong, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.34 no.4
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    • pp.273-285
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    • 2004
  • A comparative micromorphology of petal and sepal of 15 species of Lycopus was undertaken to assess their usefulness in species identification and to evaluate their significance in the taxonomy using scanning electron microscope and stereo microscope. Five types of trichome are found within the genus: unicellular cylindrical trichome, unicellular trichome with papillae, simple multicellular trichome, capitate glandular trichome, and peltate glandular trichome. The types, distribution, and density of the trichomes show considerable variation among the taxa. The distributional pattern of the unicellular cylindrical trichomes on inner side of corolla is differed among the investigated taxa, and can be classified into four patterns. The apex shape of sepal and the incised position of calyx are also various and be recognizedas four patterns. The shape of cell composing in both outer and inner sides of petal and sepal are shown variously. Finally, the taxonomic significance of micromorphology of the petal and sepal in identification and elucidation of the genus Lycopus, especially among the species is also briefly discussed.