• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.7-12
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• 2009

This study was conducted to establish the optimal suspension culture system for both the propagation of embryogenic cells (ECs) and the induction of somatic embryos (SEs) of Kalopanax septemlobus. The proliferation rate of ECs was reduced as the inoculum density was increased; the highest rate was obtained when 0.1 g/100 ml of cells was initially inoculated. According to the analysis of cell growth pattern and cell growth cycle (G1, Sand G2/M), the cell growth started in 5 days culture initiation, grew rapidly until 15 days and then decreased gradually. Distinctive changes of the cell growth cycle by the culture periods was also observed; the growth cycle was doubled from initial 5.6% to 11.7% of S stage in 5 days culture and then reached in stable stages again. Therefore, the results indicated that a 15-day-cycle was the optimal culture period for the propagation of the ECs through the suspension culture. Furthermore, the cell inoculum density was also important for the induction of SE; more than 65% of SEs at the torpedo stage was induced by using the low level of cell inoculum (0.5 g/L), while the higher inoculum densities were rapidly reduced the proportion of SEs at that stage. Although the higher inoculum density delayed the development of SE, it did not affect the proportion of SEs at the globular and heart stage. In conclusion, this study showed that the suspension culture of the Kalopanax septemlobus ECs through the control of inoculum density was an efficient way for both the propagation of ECs and the induction of SEs, suggesting that the development of this system might help to reduce the culture period for the somatic embryo production.

• The Science & Technology
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• v.34 no.4 s.383
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• pp.20-21
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• 2001

리젠바이오텍은 배ㆍ피부 등 생체조직을 파괴시키거나 이에 필요한 생리활성물질 및 촉진제를 개발하는 조직공학분야의 바이오벤처기업이다. 이 회사의 연구팀은 지난 5년동안 키토산을 이용해 각종 세포배양 연구를 하던중 최근 3차원 세포배양을 용이하게 할 수 있는 '다공성 키토산 구슬'을 개발하는데 성공했으며 지난해에는 자연치아 특성을 살린 혁신적인 인공치아 제조기술을 개발하기도 했다.

• Proceedings of the KAIS Fall Conference
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• 2003.06a
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• pp.200-202
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• 2003

배양세포에 휴대폰 전자파를 조사시켜 전자파의 영향을 연구할 수 있는 장치를 개발 제작하였다. 이 장치는 궁극적으로 이동통신용 전자파의 인체영향 평가를 위한 기초장비이며, 무선통신 산업화에 도움을 주고자 함을 목적으로 하고있다. 국내 CDMA 통화주파수인 835㎒를 발진한 신호는 세포의 종류와 인가할 세포의 SAR 레벨에 따라 다르게 전력 증폭된다. 증폭된 전자파는 CO₂ incubator 내에 설치된 TEM 균일 전자파 조사장치에 인가되고 그 안에 위치하는 petri dish내 배양세포에 균일하게 조사되도록 설계하였다. 전자파 균일 조사장치 내 전자계를 해석하고 세포의 전기적 특성에 따른 입력전력과 SAR의 관계를 구하였다.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.96-99
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• 1995

This experiment was carried out to analyze the effect of indole on the synthesis of indirubin in suspension culture of Polygonum tinctorium. Adding indole and L-tryptophan into culture media was re­vealed that indirubin was synthesized in callus grown on solid medium containing indole and proper concentration of indole for indirubin production was decided as 200mg/1. Indirubin content in suspension culture was higher than in solid medium with considerable amount of indirubin secresed into media in suspension culture and highest quantity of indirubin was obtained when indole was added into medium after 20 days suspension culture.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.57-61
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• 2000

We investigated the levels of glutathione (GSH) and its oxidized form (GSSG) in 24 cell lines derived from various plant species to understand the antioxidative mechanism in plant cell cultures. The total glutathione content was 98$\pm$27 $\mu$g/g cell fresh wt, showing a slight difference in plant species. The average contort of GSH and GSSG was 72$\pm$20 and 26$\pm$10 $\mu$g/g cell fresh wt, respectively. The average GSH content in plant cell lines occupies approximately 73% in total glutathione. During the suspension cultures of Scutellaria baicalensis, one of the plant species we tested, the GSH content decreased in proportion to the cell growth during the exponential growth stage, showing the low level at the stationary growth stage (84 $\mu$g/g cell fresh wt), whereas the GSSG content increased to the stationary growth stage (31 $\mu$g/g cell fresh wt). The results suggested that the ratio of GSH and GSSG should be involved in the cell growth and antioxidative mechanism in cultured cells.

• Korean Journal of Veterinary Service
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• v.21 no.3
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• pp.267-275
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• 1998

돼지 수정란의 체외생산은 난자의 체외성숙과 체외수정에 관한 기술의 부족으로 아직까지 만족스럽지 못한 수준이다. 특히 돼지 수정란의 체외생산에는 복잡한 세포질의 성숙과정과 높은 다정자침입율 및 전핵형성의 억제등의 문제점이 있다. 본 연구에서는 돼지 난자의 체외성숙 체계를 개선하기 위하여 transforming growth factor$\beta$(TGF$\beta$)의 첨가가 난자 및 난구세포에 미치는 효과에 대하여 검토하였다. 체외성숙용 배지에 TGF$\beta$를 1~10ng/$m\ell$의 농도로 첨가하여 미성숙 난자를 배양한 결과 성숙율이 높아졌다. TGF$\beta$의 효과는 난구세포가 제거된 난자의 성숙에도 효과적이었다. TGF$\beta$(를 첨가하지 않은 배양액 내에서는 배양 24시간 까지 metaphase-II로 성숙된 난자가 관찰되지 않았으나 TGF$\beta$를 첨가한 배양액 내에서는 관찰되었다. 한편, 난구세포가 부착된 난자의 성숙배양시 TGF$\beta$의 첨가시기에 따른 차이는 없었으나, 난구세포를 제거한 난자의 경우에는 성숙배양 전반기(59%) 또는 후반기(57%) 24시간 동안에만 TGF$\beta$를 첨가하는 것이 48시간 동안 계속하여 첨가(27%)하는 경우 또는 비첨가(38%)에 비하여 유의적으로 높은 성숙율을 나타냈다(p<0.05). 이와 같은 결과는 난구 세포가 돼지 난자의 체외성숙에 필수적이지만 TGF$\beta$는 난구세포가 제거된 난자의 체외성숙에 어 느정도 유익한 효과를 발휘하는 것으로 추측된다.

• KSBB Journal
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• v.23 no.2
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• pp.183-185
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• 2008

Studies were made to remove the toxic phytohormone, 2,4-D, in suspension cultures of Panax vietnamensis. Cells grown in normal MS medium with 2,4-D were inoculated and grown in the MS medium without hormone. Not a big difference was observed in growth characteristics between media with and without 2,4-D. The 2,4-D in the culture, however, was completely removed. During the culture, the residual 2,4-D was consumed rapidly at the early growth stage. The intra-cellular 2,4-D was consumed first and the 2,4-D in the medium was used afterward.

• KSBB Journal
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• v.15 no.5
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• pp.525-528
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• 2000

Time course monitoring of FCW/DCW ratio, production of intra and extracellular protein, and peroxidase activity were performed during suspension culture of Taxus chinensis cells. The observed FCW/DCW ratio was 12 at day 14, which was the lowest value during cultivation, and the specific protein production, based on dry cell weight, was also the lowest at day 14, which showed 4.3 mg/g DCW. The pattern of POD activity was similar to that of protein production. The results in this report were obtained using actively growing cells in flasks, therefore it is possible to use those results to control the process and indicate the stresses imposed on cells during large-scale cultivation.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.163-163
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• 2005

• KSBB Journal
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• v.14 no.2
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• pp.160-166
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• 1999

High cell density cultivation of microalga Dunaliella bardawil using nitrogen fed-batch cultures was studied in batch flask. Optimum environmental conditions include concentrated nutrients except NaCl and carbon sources, carbon sources, pH, light, agitation, nitrate and phosphate ions. Cell growth, consumption rates of nitrate and phosphate ions were monitored. Optimal conditions for higher cell density were found to be(in the range tested): 5 times concentrated media(1 times-10 times concentrated media) pH 8.0 (7.0-9.0) white light(blue and red light) 15mM of nitrate (0.94-15mM) 250mM $NaHCO_3$ and $CO_2$ gas. However, the addition of phosphate ions did not enhance the algal maximum cell density and specific growth rate. Nitrate was found to be effective for the cell growth. The maximum cell density of fed-batch culture using nitrate ions in $8.955{\times}106$cells/ml after 189hr incubation.