• Korean journal of applied entomology
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• v.61 no.1
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• pp.35-50
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• 2022

In this review, the compositions of the sex pheromones of 22 lepidopteran species were analyzed and briefly arranged by comparing the research results conducted in different locations worldwide. These compositions can be used as monitoring tools for pests of rice, maize, leguminous crops, perilla, and sesame in Korea. The sex pheromone compounds and those compositions of four species were successfully identified within Korean populations. The optimal compositions of sex pheromones of three other species were selected through behavioral studies using synthetic chemicals of the known pheromone compounds in laboratories and fields, without chemical identification processes in the sex pheromone compounds of Korean populations. For a few species, the roles of the minor components of the sex pheromone volatiles should be re-assessed for use in the development of optimal monitoring lures.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.1-14
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• 2022

Moths have a well-developed sex pheromone communication system. Male moths exhibit an extremely sensitive and selective sex pheromone detection system so that they can detect the sex pheromone produced by conspecific females and locate them for successful mating. Using the pheromone detection system, male moths display characteristic stereotypic behavioral responses, flying upwind to follow intermittent filamentous pheromone strands in pheromone plume. The chemical composition of female sex pheromone in moths, typically comprised of multiple compounds, is species-specific. Male moths contain specialized pheromone receptor neurons on the antennae to detect conspecific sex pheromone accurately, and distinguish it from the pheromones produced by other species. The signals from pheromone receptor neurons are integrated and induce relevant behavior from the male moths. Male moths also contain olfactory sensory neurons in pheromone sensilla, specialized for pheromone-related behavioral antagonist compounds, which can enhance discrimination between conspecific and heterospecific pheromones. Here we review reports on the sex pheromone detection system in male moths and their related responses, and suggest future research direction.

• Korean journal of applied entomology
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• v.39 no.2
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• pp.105-110
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• 2000

Sex pheromone composition was analyzed for Dichocrocis punctiferalis, which attacksapple, peach, chestnut fruits, etc., and its behavioral and field trapping studies were conducted to developa monitoring system with its sex pheromone. Virgin females showed maximum mating behavior andhairpencil extrusion behavior between 4-5 hrs after lights-off under 16L18D photoperiod and 26rf 1 "C.During this period, two sex pheromone components, ElO-hexadecenal and ZlO-hexadecenal, weredetected by GC analysis in the hexane extract of abdominal tips of virgin females. The best attraction ofmales to various synthetic sex pheromone blends was obtained at the 70 : 30 ratio of ElO-hexadecenal andZ10-hexadecenal for hair pencil extrusion and at the 80 : 20 ratio for flying upwind response. The highestattractiveness in fields was obtained between 70 : 30 and 80 : 20 from several tests in apple, peach andchestnut orchards.and chestnut orchards.

• Korean journal of applied entomology
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• v.44 no.1 s.138
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• pp.31-36
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• 2005

Adoxophyes spp. are the major rests of a pear. The larvae attack both leaves and fruits. (Z)-9-tetradecenyl acetate (Z9-14:Ac), (Z)-11-tetradecenyl acetate (Z11-14:Ac), (E)-11-tetradecenyl acetate (E11-14:Ac) and 10-methyldodecyl acetate (10me-12:Ac) have been reported as the sex pheromone components of the genus Adoxophyes. Our objective was to determine the difference in sex pheromone composition of three different A. spp. populations each from Cheonan, Sangju, and Naju area orchards in Korea. Gas chromatography (GC) analyses of pheromone gland extracts of virgin females confirmed the presence of two compounds (Z9-14:Ac and Z11-14:Ac) in Cheonan and Sangju populations, and four compounds (Z9-14:Ac, Z11-14:Ac, E11-14:Ac and tome-12:Ac) in Naju population. The Z9-14:Ac and Z11-14:Ac were detected in the ratio of 80:20 in the Cheonan population and 3:97 in the Sangju population. Females of Naju population produced sex pheromone blend consisting of Z9-14:Ac, Z11-14:Ac, E11-14:Ac and 10me-12:Ac at a ratio of 31:62:6:1. Field trapping tests in pear orchards with Z9-14:Ac and Z11-14:Ac indicated that maximum captures of the male were obtained with traps baited by 80:20 in Cheonan, 10:90 in Sangju, and 30:70 in Naju. These results suggest that there are remarkable geographical variations in the sex pheromone composition of A. spp. in pear orchards in Korea, and taxonomic classification of these species must be carefully assessed.

• Korean journal of applied entomology
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• v.40 no.3
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• pp.219-226
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• 2001

To identity the composition and ratio of the sex pheromone in the asiatic leafroller, Archippus breviplicanus (Walsingham), which is a major pest on apple leaves, and to develop a monitoring system using this sex pheromone, abdominal tips of their virgin female during the calling period were extracted and analysed. The GC/GC-MS analysis revealed the following three compounds; (E)11-tetradecenyl acetate (E11-14: Ac), (Z)11-tetradecenyl acetate (Z11-14: Ac), and tetradecyl acetate(14: Ac) with the ratio of 56:22:22. Electroantennogram (EAG) assay was also conducted to examine biological activity of the components. The three components evoked significantly higher responses than hexane or air but there was no significant differences between the mixtures made by components. Field trapping experiments for determining the optimum blend, seasonal fluctuation and the optimum amount for male attracting were carried out over 4 years from 1997 to 2000. Although male moths were attracted to 8:2 and 7:3 between E11-14: Ac and Z11-14: Ac, their 7:3 blend was found to be the most effective. Archippus breviplicanus appears to occur three times a year in Korea. The optimum amount for monitoring was found to be 1mg per trap. 14: Ac, the third component, did not show synergistic effect on male moth attraction.

• Korean journal of applied entomology
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• v.53 no.3
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• pp.239-246
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• 2014

Two congener species of Grapholita molesta and G. dimorpha have similar host ranges and adult occurrence patterns. In addition, the two species commonly use cis-8-dodecenyl acetate (Z8-12:Ac) and trans-8-dodecenyl acetate (E8-12:Ac) as their major sex pheromone components. The commercial lures made of the two components, therefore, suffer a mixed attraction of these two species. This study was conducted to assess any effect of their known minor sex pheromone components to reduce the mixed attraction. When cis-8-tetradecenyl acetate (Z8-14:Ac) was added to the commercial sex pheromone consisting of Z8-12:Ac/E8-12:Ac = 85/15, the attraction to G. dimorpha was not affected, but its attraction to G. molesta was significantly reduced. However, the addition of either dodecanyl acetate (12:Ac) or tetradedecanyl acetate (14:Ac) did not influence on the attraction of both species to the commercial lure. The addition of E8-14:Ac to 3-component sex pheromone, Z8-12:Ac/E8-12:Ac/Z8-14:Ac = 85/15/10, the attraction to G. dimorpha was rather significantly suppressed. From these results, the 3-component sex pheromone, Z8-12:Ac/E8-12:Ac/Z8-14:Ac = 85/15/10 was suggested as an improved composition of sex pheromone lure for G. dimorpha.

• Korean journal of applied entomology
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• v.48 no.4
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• pp.417-421
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• 2009

Monitoring was conducted to investigate the occurrence of Grapholita dimorpha Komai in Korean apple orchards using sex pheromone traps. G. dimorpa showed four peaks per year: early May, from late June to early July, from late July to mid August, and from late August to September. After adult emergence of the over-wintered G. dimorpha, G. dimorpha catches was decreased significantly and increased again after July. In G. molesta traps, G. molesta and G. dimorpha were trapped by 98.8 and 1.2%, respectively. Conversely in G. dimorpha traps, G. dimorpha and G. molesta were trapped by 99.7 and 0.3%, respectively. The 30.6% of the moths from the damaged apple fruits were G. dimorpha. This is the first report on G. dimorpha in apple orchards in Korea.

• Korean journal of applied entomology
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• v.36 no.4
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• pp.323-330
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• 1997

The apple leafminer, Phyllonorycrer ringoniella (Lepidoptera: Gracillariidae), is the most important pest on apple leaves and is not easy to be controlled with insecticides because of leafmining habit of its larvae. This study was undertaken to develop an artificial diet for mass rearing of the moth, and to look at their reproductive behavior. For developing an artificial diet the problem of diet contamination with microorganisms and how to supply artificial diet to first instar larvae have to be solved first. Artificial oviposition trial was successful with shadowy bottomside of various papers sprayed with apple leaf extracts. The highest activity of both calling and mating was observed within 30 min. after the onset of photophase and 3- to 4-day -old adults showed the highest mating rate. A ring-shaped sex pheromone gland was found at the intersegmental membrane between the 8th and 9th abdominal segments of the female. (E, 2)-4,lOtetradecadienyl acetate was identified as the major component from the femall gland extracts by the gas chromatography (GC) and GC-mass spectrometry. Additionally it was thought that (a-10-tetradecenyl acetate could be the second component of their sex pheromone.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.77-83
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• 2022

This study was conducted to determine the optimal composition of sex attractant for monitoring adults of the tobacco cutworm, Spodoptera litura, in Korea. Lures of different compositions from the two sex pheromone components the female, (Z,E)-9,11-tetradecadienyl acetate (Z9E11-14:Ac) and (Z,E)-9,12-tetradecadienyl acetate (Z9E12-14:Ac), were compared for male attraction using synthetic chemicals, rubber septa, and funnel traps in soybean and peanut fields. After sequential tests were conducted using three sets of the two components different ranges from 5:5 to 10:1, from 9:1 to 99:1, and from 9:1 to 39:1, the 19:1 ratio was identified as the most efficient composition. However, S. litura males were barely caught in the trap of the Z9E11-14Ac single component lure. The results show that both the sex attractant compounds are necessary for monitoring adults S. litura males. The higher the pheromone amount in the dispenser in the range from 0.1 to 10 mg, the more males were attracted. Furthermore, the trap height of 1.5 m was optimal for male capture.

• Korean journal of applied entomology
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• v.45 no.3 s.144
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• pp.309-316
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• 2006

Three sex pheromone components (cis-8-dodecenyl acetate (Z12Ac), trans-8-dodecenyl acetate (E12Ac), cis-8-dodecenol (Z12OH)) of the Oriental fruit moth, Grapholita molesta, were chemically synthesized. Especially to increase the composition of cis-stereoisomer, a triple bond intermediate was hydrogenated at $-20^{\circ}C$ with catalytic $Pd/BaSO_{4}$. The resulting product consisted of the acetates with a stereoisomer ratio in 92:8 (Z:E). The biological activity of the synthesized pheromone compounds was analyzed both in male responses and orientation disruption. The indoor pheromone effect was determined by male flight behavior showing wing movement in response to lure. Different mixtures of the synthetic pheromone components were prepared by mixing acetate and alcohol components in 100:0, 99:1, and 90:10 (g/g) and tested with a comparison of a standard commercial pheromone lure. The highest pheromone effect was observed in only acetate mixture (100:0) and the effect was reduced with the addition of the alcohol component. This indoor pheromone effect could be observed in field monitoring trial, in which 100:0 mixture showed the highest trap catches. Orientation disruption assay was conducted indoor by using a cage, in which the center had a commercial lure on sticky plate and the four candidates were placed at 6 cm away from the central lure on each of four directions. Test males were released to the arena during overnight (12 h) and then the caught males on the sticky plate were counted. The synthesized pheromone as well as the commercial pheromone showed 100% orientation disruption. However, the orientation disruption effect was reduced with decrease in the number of the surrounding disrupting pheromone baits. These results clearly suggest that the synthesized sex pheromone of G. molesta is biologically active and can be used for field mating disruption.