• The Korean Journal of Nuclear Medicine
• /
• v.29 no.3
• /
• pp.332-342
• /
• 1995

This study was designed to evaluate the effects of various factors on the therapeutic effect of the I-131 labeled anti-carcinoembryonic antigen monoclonal antibody(anti-CEA antibody). Tetrazolium-based colorimetric assay (MTT) was used to compare in vitro cytotoxicity of 3 Korean colon cancer cell lines (SNU-C2A, SNU-C4, SNU-C5) for selection of proper 2 cell lines in this study. The changes of the size of tumor which was xenografted to nude mice (balb/c nu/nu) were compared in 4 groups (group treated I-131 labeled anti-CEA antibody, group treated with non-radiolabeled anti-CEA antibody, group treated with I-131 labeled anti-human chorionic gonadotropin monoclonal antibody (anti-hCG antibody) as nonspecific antibody, and group injected with normal saline as a control). Immunohistochemical staining and in vivo autoradiography were performed after excision of the xenografted tumor. The results were as below mentioned. The in vitro cytotoxic effect of I-131 labeled anti-CEA antibody is most prominent in SNU-C5 cell line between 3 cancer cell lines. The changes of xenografted tumor size in both SNU-C4 and SNU-5S cell tumors at the thirteenth day after injection of the antibodies were smallest in the group treated with I-131 labeled anti-CEA antibody (SNU-C4/SNU-C5; 324/342%) comparing with other groups, group treated with anti-CEA antibody (622/660%), group treated with I-131 anti-hCG antibody (538/546%), and control group(1030/724%)(P<0.02 in SNU-C4 and P<0.1 in SNU-C5 at the 13th day after injection of antibodies). On the thirteenth day after injection of the antibodies nude mice were sacreficed to count the radiouptake of tumor and to check the changes of tumor size. Correlations between radiouptake and change of tumor size were calculated in each groups and significant negative correlation was only obtained in the group treated with I-131 anti-CEA antibody (p<0.05). There were no correlations between antigenic expression of carcinoembryonic antigen and distribution of anti-CEA antibody in both SNU-C4 and SNU-C5 cell tumors on immunoperoxidase staining. On in vivo autoradiography the distributions of anti-CEA antibody were heterogeneous and the intensities of binding were various in SNU-C4 and SNU-C5 cell tumors. It is concluded that I-131 labeled tumor-specific monoclonal antibody, anti-CEA antibody is effective in suppressing the xenografted tumor growth and the effect is influenced by sensitivity of tumor cell itself to the radiolabeled antibody and other local factors instead of specificity of antibody.

• Cross-Cultural Studies
• /
• v.51
• /
• pp.257-257
• /
• 2018

Xinxu(新序) was published in Korea by 1492. Among the existing editions, the editions that can confirm the realities are the collections of Keimyung University, the Korean Studies Central Research Institute, Kyonggi University, Hujodang(後彫堂), and the National Assembly Library of Japan. The Keimyung University's precious book is the 'first published book', and the old book is the 'later published book' which covers pages 69-70 and 71-72 of the first published book. It is the 'later published book' that has the same side inscribed. The second books, the Central Research Institute of Korea Studies and the Kyonggi University Collection are the first published books, and the Hujodang and the National Assembly Library of Japan are on pages 9-10, 63-64, 87-88, 107-108. The corresponding side is the 'later published book'. Comparing the editions, it can be concluded that the existing editions of the previous editions have been withdrawn two times, and in the latter editions, the existing editions of four editions can also be confirmed to have been edited three times. In this paper, the literature based on the existing editions was studied and features of the Korean edition were presented. First, we examine the types of paragraphs. In principle, the text is composed of '11 lines and 18 characters', but on the actual version, the number of characters is shown in the table. In the Korean edition of the Joseon dynasty, a blank space appears in the original text. The erroneous letter in the Joseon book was identified the reason for the error was explained in detail.