• Journal of Embryo Transfer
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• v.8 no.1
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• pp.25-30
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• 1993

소 난자의 시험관내 수정시 이성체인 1, 2 propanediol과 1, 3 propanediol과의 동결보호제의 이용에 대한 독성효과를 조사하였다. 프랑스에 있는 도축장의 난소에서 채취한 미성숙 난자 212개를 시험관내 성숙과 heparin(10$\mu$g/ml)첨가에 의한 수정 및 배양을 실시하였다. 소 난자의 시험관내 성숙과 수정시 propanediol의 독성효과를 시험한 결과 1,3 propanediol은 80%의 정상 난할분할율과 5.7%의 낮은 다핵분할구율을 나타내었다. 이것은 1,2 propanediol의 73.9%, 10.3%와 미처리대조구의 73.9%, 7.7%와 비교되었으나 처리간의 유의성은 없었다. Heparin 처리구는 1,2 propanediol의 첨가 유무에 관계없이 미처리구에 대하여 고도의 유의성 차이가 인정되었다. (P<0.05)

• Korean Journal of Ichthyology
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• v.35 no.4
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• pp.333-339
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• 2023

The aim of this study was to collect information regarding the sex ratio, size at sexual maturity, and the primary spawning period of Hexagrammos otakii. The sex ratio (female : male) was observed to be 1 : 1.2 (n =739 : 906, with females comprising 81.6% of the population). The gonadosomatic index (GSI) exhibited its highest values in November for females and October for males, coinciding with the primary spawning period from November to January. The total length required for 50% sexual maturity was determined through a logistic regression model and found to be 23.1 cm for females.

• The Journal of the Korea Contents Association
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• v.18 no.12
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• pp.197-207
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• 2018

This study analysis effect of social support and career maturity on readiness for independent living of run away youths using drop in center. This result peer support had more influence on preparing for independent living than in supporting the working class and career maturity had a huge impact on all the low-variables of preparing for independent living. Career maturity level for run away youths paly an important role in preparing for independent living just like general youths. On the base this study propose supporting system preparing for independent living, worker empowerment, drop in center's role establish for independent living.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.39 no.3
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• pp.174-180
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• 2003

This study investigated the size selectivity of the round traps for greenling (Hexagrammos otakii) in the western sea of Korea. The selection curve for the greenling from the experiments on Oct. 2000 and Ar. 2001 was fitted by Kitahara's method to a polynomial equation and two parameter logistic selection curve. The selectio curve of the latter was more reasonable than that of the former. The equation of selectivity curve obtained using a logistic function with least square method was , s(R)=1/1+exp(-1.1169R+6.4565), where R=1/m, and 1 and m are total length and mesh size, respectively. The size selectivity curve showed that the current regulated mesh size(35mm) in case of the round trap was close to the L50 (37.0mm) of the selection curve for the biological minimum length (21.4cm) of the greenling.

• Reproductive and Developmental Biology
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• v.28 no.2
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• pp.95-99
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• 2004

This study was carried out to assess whether the addition of myo-inositol to maturation medium could improve porcine oocyte maturation in vitro. Oocytes were cultured for the first 22 h in Witten's medium containing 10IU/$m\ell$ PMSG, 10 IU/$m\ell$ HCG supplemented with or without myo-inositol. Subsequently, they were cultured for additional 22 h in Witten's medium without hormone supplemented with or without myo-inositol. When the porcine oocytes were cultured in maturation medium containing myo-inositol, the proportion of metaphase II oocytes 44h after culture was higher in the myo-inositol group(P<0.05). To study effects of cumulus cell on the maturation induced by myo-inositol, we examined the maturation status of cumulus-enclosed or cumulus-denuded porcine follicular oocytes. The rates of maturation were significantly higher in the cumulus-enclosed oocytes(P<0.05). However, the maturation rates of cumulus-denuded oocytes cultured in medium containing myo-inositol were higher than those of control group(P<0.05). Our results suggest that myo-inositol may affect meiotic progression of porcine follicular oocytes and supplementation of myo-inositol in maturation medium may be useful for the in vitro maturation of porcine follicular oocytes.

• Journal of Embryo Transfer
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• v.19 no.3
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• pp.229-237
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• 2004

The main objective of this study was to examine the effects of serum and gonadotropins supplement during in vitro maturation(IVM) of bovine oocytes on nuclear maturation and embryo development, and we also examine the cell number. 1 . The first polar body(PB) extrusion rates of Korean native cow(KNC) oocytes matured in medium with FBS or gonadotropins were similar among treatment groups. The development rate to the blastocyst stage was significantly higher in the group of both supplement FBS and gonadotropins(26.0％) than in the group of non-supplement(9.9％) and gonadotropins (12.0％). The numbers of inner cell mass (ICM) and trophectoderm (TE) cells and total cell numbers of blastocysts were highest in the group of both supplement FBS and gonadotropins, and the number of ICM cells was increased by FBS supplementation (p<0.05). 2. The PB extrusion rates of KNC oocytes matured in medium with FBS in the different duration of IVM was significantly higher in the 0-18hr(63.1％) and in the 9-18hr(63.4％) group than in the 0-9hr.(37.4％) group (p<0.05). The embryo development rates did not differ among treatment groups. The numbers of TE cells and total cell numbers of blastocysts were similar among treatment groups, but the number of ICM cells of the 0-18h. group were significantly higher than the other treatment groups (p<0.05). The results indicate that although TCM199 alone can support bovine oocyte maturation and development to the blastocyst stage, a high quality of blastocysts can be produced from oocytes matured in medium containing serum and gonadotropins.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.4
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• pp.538-544
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• 2021

Technology readiness assessment is a procedure for managing defense project risk factors based on the preemptive identification of technical risks. Under current regulations, technology readiness is determined based on considerations of the ratings of factors itemized on a checklist, whether unsatisfied factors have a fatal impact on the project, and whether countermeasures for unsatisfied factors have been established. However, objective criteria for assessing the impact of unsatisfied factors have not been presented, and thus, at present, the results of technology readiness level determinations are largely subjective. In addition, the importance of questions on the checklist is dependent on individual project characteristics and this is not considered during the assessment process. In this paper, we propose an improved technology readiness assessment procedure that considers the characteristics of each project. Using the proposed procedure, we quantitatively determined the importance of each checklist item using a weighting method. We found the devised procedure improved the reliability and objectivity of technology readiness assessment results. A case analysis of a complex weapons system is presented to demonstrate these improvements.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.95-95
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• 2003

Inositol은 세포의증식 및 정보전달과정에 관여하는 phosphatidylinositol (PI)의 구성성분으로서 중요한 세포내 기능을 수행한다. P1는 세포내에서 특이적 인산화효소에 의하여 Pl4-phosphate(PIP), Pl4,5-phosphate($PIP_2$)로 변환되며 PIP$_2$는 phospholipase C(PLC)에 의하여 세포내 second messengers인 1,2-diacylglycerol (DAG)와 inositol 1,4,5-triphosphate ($IP_3$)로 변환된다. 이렇게 생산된 DAG와 IP3는 각각 protein kinase C의 활성과 $Ca^{2++}$의 동원에 관여하여 다양한 세포내 신호전달에 관여하는 것으로 보고되고 있다. 또한 mouse에서 $IP_3$의 작용에 의한 $Ca^{2++}$의 상승은 난모세포의 성숙분열이 촉진되고 돼지 난포세포에 있어서도 PI대사가 일어나고 있는 것으로 보고되고 있다. 본 연구에서는 돼지 미성숙 난모세포의 성숙과 단위발생에 미치는 inositol의 영향을 확인하기 위하여 실시하였다. inositol의 농도가 난포란의 성숙에 미치는 영향을 검토하기 위하여 난포란을 각각 $150 \mu mole$, $250 \mu mole$, $350 \mu mole$, inositol을 포함하는 Whitten's 배양액에서 44시간 성숙시킨 결과 $93.57 \pm 4.21, 93.91 \pm 2.71, 92.96 \pm 3.58%$가 성숙되어 대조구의 $87.10 \pm 4.21$보다 유의적(P<0.05)으로 차이를 나타내었다. 난포란의 등급에 따른 inositol의 영향을 확인하기 위하여 형태적으로 난구세포가 치밀한 난포란과 난구세포가 치밀하지 않은 난포란을 $250 \mu mole$ inositol을 포함하는 Whitten's 배양액에서 성숙을 유도한 결과 난구세포가 치밀한 난포란과 난구세포가 치밀하지 않은 난포란에서 inositol을 첨가하였을 때 성숙율은 각각 $95.35 \pm 2.22와 63.55 \pm 8.12$로 inositol을 첨가하지 않은 대조구보다($89.21 \pm 3.69와 48.56 \pm 8.99$) 유의적인 차이를 보였다. 난구세포가 inositol에 의한 성숙에 미치는 영향을 확인하기 위하여 난구세포를 제거한 난포란을 inositol을 포함하는 배지에서 성숙을 유도한 결과 inositol을 첨가하지 않은 처리구보다 양호한 성숙율을 보였다.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.05a
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• pp.341-342
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• 2003

쥐노래미는 쥐노래미과에 속하는 연안정착성 동계산란어종으로 우리나라 전연안과 중국, 일본에 서식하고 있다. 최근 무분별한 남획으로 인하여 자원량이 급감되어 쥐노래미는 일찍부터 양식대상종으로 각광을 받아왔다. 지금까지 쥐노래미과에 관한 연구ㆍ보고된 것은 정과김(1994)에 의한 노래미의 성숙과 산란이 보고되어 있을뿐 쥐노래미의 성성숙과 산란에 관한 연구는 찾아보기 어렵다. (중략)

• 한국벤처창업학회:학술대회논문집
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• 2016.04a
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• pp.118-122
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• 2016

북한이탈주민의 입국자가 곧 3만 명에 육박할 것이다. 이로 말미암아 북한이탈주민에 대한 한국사회에서의 안정된 정착이 큰 문제로 대두되고 있기에, 그 문제들을 해결하고자 창업교육을 실시하여 북한이탈주민의 경제적 고립을 막고, 한국사회에서 올바른 진로를 선택하여 미래 통일 역군을 만들고자 한다. 본 논문은 창업교육 전, 후 창업동기 및 의지, 기업가정신, 진로장벽, 진로성숙도, 진로미결정 요인을 분석하여 창업교육에 대한 효과성을 강조하고자 한다. 아울러 장지적으로는 국내 입국 후 북한이탈주민들이 느끼는 심리를 분석하여 그들이 진정으로 원하는 진로와 정부지원정책을 도출해 내고자 한다.