• Journal of Life Science
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• v.29 no.6
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• pp.653-661
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• 2019

The first small interfering RNA (siRNA) therapeutics have recently been approved by the Food and Drug Administration in the U.S., and the demand for a new RNA therapeutics bioanalysis method-which is essential for pharmacokinetics, including the absorption, distribution, metabolism, and excretion of siRNA therapeutics-is rapidly increasing. The stem-loop real-time qPCR (RT-qPCR) assay is a useful molecular technique for the identification and quantification of small RNA (e.g., micro RNA and siRNA) and can be applied for the bioanalysis of siRNA therapeutics. When the anti-HPV E6/E7 siRNA therapeutic was used in preclinical trials, the established stem-loop RT-qPCR assay was validated. The limit of detection was sensitive up to 10 fM and the lower limit of quantification up to 100 fM. In fact, the reliability of the established test method was further validated in three intra assays. Here, the correlation coefficient of $R^2$>0.99, the slope of -3.10 ~ -3.40, and the recovery rate within ${\pm}20%$ of the siRNA standard curve confirm its excellent robustness. Finally, the circulation profiles of siRNAs were demonstrated in rat serum, and the pharmacokinetic properties of the anti-HPV E6/E7 siRNA therapeutic were characterized using a stem-loop RT-qPCR assay. Therefore, the stemloop RT-qPCR assay enables accurate, precise, and sensitive siRNA duplex quantification and is suitable for the quantification of small RNA therapeutics using small volumes of biological samples.

• Journal of the Korean Institute of Gas
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• v.8 no.2 s.23
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• pp.15-27
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• 2004

The purpose of this paper is to improve the performance of Polymer electrolyte fuel cell(PEMFC) by studying the channel dimension of bipolar plates using commercial CFD program 'Fluent'. Simulations are done ranging from 0.5 to 3.0mm for different size in order to find the channel size which shoves the highst hydrogen consumption. The results showed that the smaller channel width, land width, channel depth, the higher hydrogen consumption in anode. When channel width is increased, the pressure drop in channel is decreased because total channel length Is decreased, and when land width is increased, the net hydrogen consumption is decreased because hydrogen is diffused under the land width. It is also found that the influence of hydrogen consumption is larger at different channel width than it at different land width. The change of hydrogen consumption with different channel depth isn't as large as it with different channel width, but channel depth has to be small as can as it does because it has influence on the volume of bipolar plates. however the hydrogen utilization among the channel sizes more than 1.0mm which can be machined in reality is the most at channel width 1.0, land width 1.0, channel depth 0.5mm and considered as optimum channel size. The fuel cell combined with 2cm${\times}$2cm diagonal or serpentine type flow field and MEA(Membrane Electrode Assembly) is tested using 100W PEMFC test station to confirm that the channel size studied in simulation. The results showed that diagonal and serpentine flow field have similarly high OCV and current density of diagonal (low field is higher($2-40mA/m^2$) than that of serpentine flow field under 0.6 voltage, but the current density of serpentine type has higher performance($5-10mA/m^2$) than that of diagonal flow field under 0.7-0.8 voltage.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.11
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• pp.520-526
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• 2019

I-type girders are widely applied as very economical sections in plate girder bridges. There has been research on developing composite laminated panels, curved plates reinforced with closed-end ribs, and new forms of ribs and compression flanges for steel box girders. However, there is a limitation in analyzing the exact cause of local buckling caused by an I-type girder's webs. Therefore, an I-type girder's web was modeled using the finite element analysis program LUSAS 17.0 before and after reinforcement. We checked for the minimum thickness criteria presented in the Korea highway bridge design code, and the cause of buckling after performing a linear elastic buckling analysis of dead and live loads was analyzed. Before reinforcement, an eigenvalue (λ₁) at the 1st mode was 0.7025, the critical buckling load was smaller than the applied load, and there is a buckling. After reinforcement, when applying vertical and horizontal stiffeners to the web part of the girder at support, a Nodal line was formed, the eigenvalue was 1.5272, and buckling stability was secured. To improve buckling trace of the girder at the support, an additional plate was applied to the web at the support to ensure visual and structural safety, but buckling occurs at center of web. The eigenvalue (λ₁) was 3.5299, and this method is efficient for reinforcing the web of the support.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.5
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• pp.191-205
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• 2020

This study examined the possibility of utilizing next-generation technology, such as Virtual Reality or AI robots, in the long-term care facilities for the elderly. For the study, the Focus Group Interview was conducted in three different groups of 14 participants (care workers, social workers, and directors of long-term care facilities for the elderly). The analysis revealed a total of three topics, eight categories, and 26 sub-categories. The main results of the study showed that the use of next-generation technology could assist the psychological and emotional stability, provide curiosity and interest, and relieve the desire for physical activity for the elderly. In addition, for long-term care services staff, it could provide useful services for the elderly with physical constraints, facilitate effective management of the elderly roaming around, and enhance emotional support services. Finally, it could also help directors of long-term care facilities promote their services, educate staff, and keep up with current trends. Participants expressed concerns about the introduction of new technologies, but they generally expected that the application of next-generation technology would be positive for the elderly as well as for care workers and directors of long-term care facilities. Therefore, the use of next-generation technology in long-term care facilities for the elderly will also help develop gerontechnology.

• Journal of Navigation and Port Research
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• v.33 no.5
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• pp.315-321
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• 2009

The Very Large Marine Structure has been widely used new method of ocean space instead of method for reclamation Therefore, VLFS is proposed to coincide on such request. It can be established regardless of nature of soil and height of water, and stream of flow exists under the floating structure, there is seldom effect in natural environment. Fuertherrnore, it can do easily to do assembly and taking to pieces due to expansion or removal. Based on the regulation by class, VLFS have to possess more than enough structural strength against severe wave loading induced by green sea condition Therefore, There are performed structural simulation as well as experimental test about expected loading scenario in order to examine the safety of structure. Up to now, various examinations based on the strength limit value of the main structural material have been done based on the elasticity response analysis. However, there is little finding about the collapse behavior and the safety when the load that exceeds the collapse of the material acts. In the present study, we investigated the collapse behavior based on the ultimate limit state calculated by FE-analysis.

• Journal of the Korean Chemical Society
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• v.55 no.1
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• pp.63-69
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• 2011

This work discusses the fabrication, development and potential response behaviors of $Ag^+$ ion-selective electrodes ($Ag^+$-ISE) based on N,N'-bis(2-hydroxybenzyl)-1,3-diaminopropane (L1) and N,N'-bis(2-hydroxybenzyl)-2,2-dimethyl-1,3-diaminopropane (L2) as carriers. The observations indicated that the resulting electrode based on L1 toward $Ag^+$ showed stable near-Nernst slope approaching 58.7 mV/dec and the optimum potential response characteristics in a linear range at least five orders of magnitude with a detection limit of $1.0{\times}10^{-6}M$. The proposed electrode displayed the preferential selectivity to $Ag^+$ against other tested cations. The excellent potential analytical characteristics could lead to the successful applications of silver assay in significant real samples, indicating that the proposed $Ag^+$-ISE showed a significant advancement of measurement capabilities. But for the electrode based on L2, the poor potential response characteristics were observed in total experiment process.

• The Korean Journal of Petroleum Geology
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• v.14 no.1
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• pp.45-52
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• 2008

The GTL(Gas to Liquids) technology, manufacturing synthesized oil from natural gas, had been developed about 1920 for the military purpose by Fischer and Tropsch, German scientists. And 1960, Sasol company had started commercializing the FT(Fischer-Tropsch) synthesis technology, for the transport fuel in South Africa. Until a recent date, the commercialization of GTL technology had been delayed by low oil price. But concern about depletion of petroleum resources, and development in synthesizing technology lead to spotlight on the GTL businesses. Especially, Qatar, which has rich natural gas fields, aims at utilizing natural gas like conventional oil resources. Therefore, around this nation, GTL plants construction has been promoted. There are mainly 3 processes to make GTL products(Diesel, Naphtha, lube oil, etc) from natural gas. The first is synthesis gas generation unit reforming hydrogen and carbomonoxide from natural gas. The second is FT synthesis unit converting synthesized gas to polymeric chain-hydrocarbon. The third is product upgrading unit making oil products from the FT synthesized oil. There are quite a little sulfur, nitrogen, and aromatic compounds in GTL products. GTL product has environmental premium in discharging less harmful particles than refinery oil products from crude to the human body. In short, the GTL is a clean technology, easier transportation mean, and has higher stability comparing to LNG works.

• Journal of the Korean Geosynthetics Society
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• v.17 no.4
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• pp.1-10
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• 2018

Although the soil nailing method is generally used as a gravity grouting, the development and application of pressurized grouting method has recently increased to address the problem of joint generation and filling due to grouting. Pressurized grouting of the soil nailing method is generally used in combination with ordinary portland cement and water. In the field, the cement is mixed with the rapid setting cement to reduce curing time because ordinary portland cement takes more than 10 days to satisfy the required strength. In this study, uniaxial compression tests and laboratory tests were carried out to confirm the efficiency of the grouting material according to the mixing ratio of rapid setting cement. The mixing ratio of 30% grouting satisfies the required strength within 7 days and satisfies the optimum gel time. As a result of the laboratory test with granite weathered soil, the reinforcing effect was confirmed to be 1.5 times as compared with the gravity type at an injection time of 10 seconds and a strain of 15%. The friction resistance increases linearly with the increase of the injection time, but it is confirmed that the friction resistance decreases due to the hydraulic fracturing effect at the injection time exceeding the limit injection pressure. Numerical analysis was performed to compare the stability of slopes not reinforced with slopes reinforced with gravity and pressurized soil nailing.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.40 no.2
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• pp.119-133
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• 2022

Due to the improvement of surveying, mapping and communication techniques, various apps for road direction guides and vehicle navigations have been developed. Although such a development has impacted on walking and driving, there is a limit to improving the daily convenience of the socially impaired people. This is mainly due to the fact that the software have been developed for normal pedestrians and drivers. Therefore, visually impaired people still have problems with the confusion of direction and/or non-provision of risk factors in walking. This study aimed to propose a scheme which constructs data for mobility-impaired or traffic-impaired people based on various geospatial information. The factors and components related to walking for the visually impaired are selected by geospatial data and a walking route guidance network that can be applied to a commercial software. As a result, it was confirmed that road direction guidance would be possible if additional contents, such as braille blocks (dotted/linear), sound signals, bus stops, and bollards are secured. In addition, an initial version of the application software was implemented based on the suggested data model and its usefulness was evaluated to a visually impaired person. To advance the stability of the service in walking for the visually impaired people, various geospatial data obtained by multiple institutes are necessary to be combined, and various sensors and voice technologies are required to be connected and utilized through ICT (Information and Communications Technologies) technology in near future.

• Korean journal of applied entomology
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• v.61 no.4
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• pp.563-575
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• 2022

Maruca vitrata is one of important pests in leguminous crops, especially red bean. We investigated the effects of temperature on development of each life stage, adult longevity and fecundity of M. vitrata for understanding the biological characteristics of the insect species at eight constant temperatures of 13, 16, 19, 22, 25, 28, 31, and 34℃. Eggs hatched successfully at all temperature subjected and larvae successfully developed to the adult stage from 16℃ to 31℃. The developmental period of egg decreased up to 31℃ and after then increased. The developmental period of larva and pupa, and adult longevity of M. vitrata decreased with increasing temperature. Lower and higher threshold temperature (TL and TH) were calculated by the Lobry-Rosso-Flandrois (LRF) and Sharpe-Schoolfield-Ikemoto (SSI) models. The lower developmental threshold (LDT) and thermal constant (K) from egg hatching to adult emergence of M. vitrata were estimated by linear regression as 12.8℃ and 280.8DD, respectively. TL and TH from egg hatching to adult emergence using SSI model were 14.2℃ and 31.9℃. Thermal windows, i.e., the range in temperature between the minimum and maximum rate of development, of M. vitrata was 17.7℃. In addition, we constructed the oviposition models of adult, using the investigated adult traits including survival, longevity, oviposition period and fecundity. Temperature-dependent development models and adult oviposition models will be helpful to understand the population dynamics of M vitrata and to establish the strategy of integrated pest management in legume crops.