• Journal of the Korean Society of Tobacco Science
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• v.18 no.1
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• pp.39-48
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• 1996

A storage protein(SP-2) was confirmed in haemolymph during larval-pupal-adult development of tobacco burdworm(Helicoverpa assulta Guenee), and its biochemical characteristics were investigated. The titer of SP-2 showed a peak at mature larva, decreased gradually through the late pupal stage, and became undetectable at adult period. As the results from electrophoretic mnysis, SP-2 was confirmed to be glycolipoprotein(M.W. 332kDa) relatively stable to heat( $\leq$ 68$^{\circ}C$ ). This storage protein was determined to be a tetramer composed of a single subunit with MW of 83kDa, and the isoelectric point was 5.7. The amino acid composition of the SP-2 was characterized. It has relatively high content of methionine and histidine, whereas the contents of tyrosine and phenylalanine were relatively low.

• Toxicological Research
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• v.14 no.2
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• pp.177-181
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• 1998

Dithiocarbamate and mixed disulfide containing allyl functions were designed and synthesized as putative chemopreventive agents, i.e. N,N-diallyldithiocarbamate (DATC) and S-(N,N-diallyldithiocarbamoyl)-N-acetylcysteine (AC-DATC). DATC and AC-DATC were administered and the activities of cytosolic glutathione S-transferase (GST), glutathione reductase (GR) and microsomal N-nitrosodiethylamine (NDEA) deethylase were assayed in order to test the effects of these organosulfur com-pounds on the detoxification and metabolic activation system of NDEA. The amounts of hepatic glutathione (GSH and GSSG) was also determined. The administration of DATC to rats led to an increase in the activity of GR and to an inhibition of CYP2E1-mediated NDEA deethylation. AC-DATC induced the activity of GR and GST, increased the hepatic GSH content and inhibited the rate of NDEA deethylation. The level of GSSG was decreased as a consequence of the increased activity of GR. These effects may contribute to possible antimutagenic and anticarcinogenic action of the dithiocarbamates investigated.

• Korean Journal of Veterinary Research
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• v.28 no.1
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• pp.75-81
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• 1988

The present study was conducted to investigate the Bordetella bronchiseptica infection in Youngnam swine herds during the period from August 1986 to July 1987 and some properties of the organisms isolated from these Korean swine. B. bronchiseptica was recovered from 25 of 70 (35.7%) glowing pigs of 4 to 10 weeks of age and from 12 of 13 (92.3%) herds. From 115 slaughter pigs, 58(50.4%) pigs were culture positive and the pigs from 13 of 14 (92.9%) herds were found to be infected with B. bronchiseptica. The majority of biochemical and cultural properties of B. bronchiseptica isolated from Korean swine were identical to those of the standard strain employed and some 97.6% of the isolates showed the characters of phase I organism on primary isolation.

• Electronics and Telecommunications Trends
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• v.25 no.5
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• pp.59-71
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• 2010

피부질환 치료를 위한 광선요법은 태양광, 레이저, 형광등, UV 램프 등 다양한 광원을 이용하여 광이 피부 내에서 생화학적 반응을 촉진하는 원리를 이용하여 피부 조직의 선택적 재생 또는 파괴 등을 통해 손상된 피부를 치료하는 광 의료 기술이다. 최근 발전하고 있는 LED 광원 기술은 광선요법에서 시용되어온 광원을 대체할 수 있는 기술로 각광을 받고 있다. 이에 본 고에서는 광선요법과 관련된 기초 원리를 살펴보고, LED가 피부질환 치료용 광원으로 사용될 때 고려되어야 하는 임상적, 기술적 문제점과 다양한 피부질환 치료에 있어서 LED 광원의 역할에 대해 살펴 보았다. 아울러, 피부질환 치료용 LED 치료기 관련 국내외 연구 개발 동향과 기업들이 출시한 LED 치료기의 특성을 살펴보았다. LED 치료기 관련 표준화 동향과 국내외 지식재산권 현황을 살펴보았으며, 향후 LED 기반의 피부질환 치료기의 개발 방향을 모색하였다.

• Korean Journal of Animal Reproduction
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• v.16 no.1
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• pp.1-6
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• 1992

These experiments were undertaken as a basic study to understand the role of cumulus cell on in vitro maturation and fertilization process with identifying the cumulus cell-secreted proteins. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and fast protein liquid chromatography(FPLC), the proteins of cumulus cells were identified. The results obtained in these experiments were summarized as follows ; 1. When the proteins secreted from cultured cell for 30 hours were separated by SDS-PAGE, there were a major band (>94,000) and other minor 2 bands with molecular weight ranging 30,000∼67,000 dalton. 2. In addition, the fractionations of these proteins by FPLC were idirectly shown that three bands were hyaluronic acid, chondroitin sulfate, and heparin.

• Korean Journal Plant Pathology
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• v.1 no.1
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• pp.38-43
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• 1985

A bacterial brown stripe disease new to Korea was noted for the first time on rice seedlings grown in the nursery pots and in a field nursery. Artificial hypodermic injection and pricking inoculation with isolates obtained from lesions of naturally infected plants produced symptoms similar to those occurring under natural condition. Among eleven species of ten genera of gramineous plants Echinochloa crusgalli, Digitaria sanguinalis and Setaria viridis were the new hosts for the pathogen. On the basis of bacteriological and biochemical tests of isolates from infected rice seedlings, the causal bacterium is considered to be Pseudomonas avenae Manns.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.312-317
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• 1995

The polygalacturonase (PG) production in rotten apples by Botryosphaeria dothidea was purified by using gel filtration and ion exchange column chromatography, and the biochemical characters of PG were investigated. The purified PG appeared as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with approximate molecular weight of 49 kilodalton (kDa). The molecular weight was equal to the native molecular weight estimated by gel filtration. The Km and Vmax values of PG were 0.51 mg/ml and 90.9 $\mu$M/min/ml, respectively. Optimum pH was 4.0~5.0, and the PG activity was stable from pH 5.0~10.0. Optimum temperature of the enzyme activity was 4$0^{\circ}C$. The PG activity was relatively stable at 2$0^{\circ}C$, but it was reduced 45% at 4$0^{\circ}C$ and completely inactivated at 8$0^{\circ}C$. The PG activity was considerably inhibited by Cu2+, Zn2+, SDS and EDTA, whereas it was not effected by Ca2+, K+, Mg2+ or Na+ ions.

• 대한예방의학회:학술대회논문집
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• 2005.10a
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• pp.355-355
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• 2005

• Korean Journal of Veterinary Service
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• v.14 no.2
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• pp.121-126
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• 1991

The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 36 strains of Pasteurella haemolytica(P haemolytica) isolated from pneumonic calves in Kyongbuk province during the period from January 1990 to December 1990. P haemolytica was isolated from 36 of 111(32.4%) pneumonic calves of 1 to 6 months of age. The majority of biochemical and cultural properties of P haemolytica isolated from calves were identical to those of the reference strains employed. All isolated were susceptible to baytril, gentamicin, and kanamycin, some of them were resistant to tetracycline, amikacin, streptomycin, and ampicillin.

• The Microorganisms and Industry
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• v.14 no.1
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• pp.22-28
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• 1988

DNA 클로닝과 조작기술의 발전은 어떤 유전자의 특정한 위치에 선택적으로 돌연변이를 도입할 수 있는 site-specific mutagenesis 기술을 창출해 내었다. 이 기술로 DAN 염기의 치환, 결실, 삽입등을 클론된 유전자에 직접 도입할 수가 있게 되어 생체의 유전자 조작이나 유전자의 산물인 단백질의 구조와 기능을 의도적으로 변화시키는 protein engineering에 광범위하게 이용되고 있다. Protein engineering은 주로 단백질의 촉매 및 생리활성의 증가, 효소의 특성및 기질 특이성의 변화, 단백질 구조의 안정화 및 내염성 증가, 분자량의 감소, 효소및 생리활성 단백질의 구조의 안정화및 내열성 증가 등에 활용되고 있으며 산업적 유용성이 큰새로운 단백질의 창조에도 기여할 것으로 기대를 모으고 있다. Site-specific mutagenesis 기술로 현재 가장 널리 이용되는 것이 in vitro상에서 수행하는 oligonucleotide-directed site specific mutagenesis이다. 이 방법은 생화학적으로 합성한 특정한 염기서열을 가진 oligonucleotide들을 일종의 mutagen으로 사용하거나 효소적 DNA 합성을 위한 primer로 사용하여 클론된 DNA의 염기서열을 선택적으로 개조하거나 혹은 다른 조작을 하는 것이다. 여기서는 돌연변이율을 높이는 여러가지 개량된 방법들이 나왔으며 그중의 몇가지를 소개하였다.