• Journal of Korean Society on Water Environment
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• v.22 no.6
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• pp.1123-1129
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• 2006

Pyrene of natural and anthropogenic sources is one of the toxic, mutagenic polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants. The objectives of this research are to break down pyrene by using ozonation, identify the intermediates and byproducts of pyrene, and test the biodegradability of intermediates and byproducts of pyrene in the aqueous phase. Since pyrene is non-polar, hexane was chosen as a solvent to effectively dissolve pyrene. Pyrene solutions were treated with ozone, as it has high oxidation capacity and electrophilic characteristic. After different ozonation pretreatment times (2, 3, and 10 minutes), intermediates of pyrene in the form of yellowish solid were collected from the hexane solution using a centrifuge. They were identified by gas chromatography/mass spectrometer (GC/MS). $BOD_5$, COD, and E-coli toxicity tests have been performed to assess the ozonation products.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.5
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• pp.565-569
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• 1993

Antioxidative activity of browning product(BP) fractionated from fermented soybean sauce(SS) was studied during the oxidation process of linoleic acid mixture system. SSBP was a powder type product prepared from fermented soybean sauce by the fractionation through the Sephadex G-10 column and freeze drying of collected fraction. The aqueous model systems were used for the evaluation of antioxidative activity of SSBP during the oxidative reaction at $50^{\circ}C$ by the determination of peroxider and conjugated dienoic acid compounds. The linoleic acid mixture for the aqueous model systems was consisted of linoleic acid(64.6%), oleic acid(27.4%), and other acids in ethanolic phosphate buffer solution(pH 7.0). SSBP had a considerable antioxidative activity with the inhibition of formation of peroxides and conjugated dienoic acids during the autoxidation of linoleic acid mixtures in aqueous model systems. Antioxidative activity of SSBP was relatively higher than SS, however, lower than ${\alpha}-tocopherol$ and butylated hydroxyanisol. The antioxidative effect of SSBP was increased by the its concentrations from 0.05% to 0.5% in the oxidation reactions of aqueous model systems. Therefore, SSBP was considered as one of the potential natural antioxidants for the use of food products.

• Journal of the Mineralogical Society of Korea
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• v.23 no.4
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• pp.315-329
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• 2010

Characteristics of thermophilic bacteria and secondary materials on the pyrrhotite surface were investigated by using scanning electron microscopy (SEM). The thermophilic bacteria from an acid hot spring in Japan were incubated with pyrrhotite at $42^{\circ}C$, $52^{\circ}C$, and $62^{\circ}C$ respectively. SEM analysis of the reacted pyrrhotite showed that indigenous rod-shaped bacteria ranging from $0.4{\times}1.5{\mu}m$ to $0.3{\times}11.9{\mu}m$ in size were attached to the pyrrhotite surface at these temperatures with formation of secondary materials. Extracellular polymer substances were formed on the bacterial surface. We suggest that these polymers functioned as a capsule protecting bacteria from the extreme environment. Secondary materials such as elemental sulfur, Fe-hydroxide, S-Fe and O-P-Fe compounds were found on the pyrrhotite surface.

• Korean journal of food and cookery science
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• v.20 no.5
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• pp.505-510
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• 2004

이물질 침입에 대한 인식의 결과 NO, PGE$_2$, TNF-, IL-6와 같은 여러 신호전달물질의 분비가 개시되며 이들을 억제하는 물질을 항염증제라고 볼 수 있다. 본 연구에서는 회향(Foeniculum vulgare Mill.) 열매 추출물이 mouse macrophages RAW264.7 세포에서 lipopolysaccharide(LPS)로 유도한 NO(iNOS 산물), PGE$_2$(COX-2 산물) 및 cytokines (TNF-$\alpha$, IL-6) 생성 억제에 미치는 영향을 살펴보았다. 회향 열매의 methanol 추출물 및 분획물(chloroform, butanol, and aqueous fractions)은 4～100$\mu$g/mL 농도에서 LPS가 활성화된 대식 세포에서 NO 생성을 억제하였으며 독성을 나타내지 않았다. LPS가 유도한 PGE$_2$ 생성은 butanol 분획(100 $\mu$g/mL)에 의해서만 유의적으로 감소하였다(P<0.05). 회향 열매 추출물 및 분획물은 TNF-$\alpha$의 생성을 유의적으로 감소시켰으며 IL-6의 생성은 methanol extract(4～100 $\mu$g/mL), chloroform fraction(4 $\mu$g/mL), butanol fraction(4 and 100$\mu$g/mL) 및 aqueous fraction(4～100 $\mu$g/mL)에 의해 감소되었다(P<0.05). 이는 회향 열매 추출물은 염증 상태에서 유용할 것이며 COX-2와 iNOS를 억제하는 butanol 분획은 새로운 항염증제 개발에 사용될 수 있음을 시사하여 주었다.

• Journal of Food Hygiene and Safety
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• v.13 no.3
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• pp.243-251
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• 1998

Panax ginseng C.A. Meyer has been extensively used in the traditional oriental medicine as a restorative, tonic and prophylatic agent. Petroleum ether extract of panax ginseng C.A. Meyer (GPE) and its several fractions (PI-P5) were tested for the evaluation of antigenotoxicity against N-methyl-N-nitrosourea (MNU) and benzo(a)pyrene [B(a)P]-induced micronucleated reticulocytes in mouse peripheral blood. GPE and P2 showed more significant anticlastogenicity than other fractions did. To elucidate the anticlastogenic action mechanism of GPE and P2 against B(a)P, the alteration of B(a)P metabolism was studied. GPE and P2 inhibited B(a)P metabolism in the presence of 8-9 mix and decreased B(a)P-DNA binding in calf thymus DNA with 8-9 mix. They also decreased [$^3H$] MNU induced DNA binding and methylation to 7-methyl guanine and $O^{6}-methyl$ guanine adducts in calf thymus DNA by RPLC analysis. These results suggest that the anticlastogenicity of GPE and P2 on the B(a)P or MNU-induced clastogenicity is due to decrease of DNA binding with B(a)P or MNU, the inhibition of metabolism with B(a)P and the inhibition of methylation in DNA. Therefore, GPE and P2 may be useful chemopreventive agents of alkylating agent like MNU and secondary carcinogen like B(a)P.

• 월간산업보건
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• s.78
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• pp.20-26
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• 1994

• 월간산업보건
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• s.77
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• pp.16-18
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• 1994

• 월간산업보건
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• s.76
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• pp.21-26
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• 1994

• 월간산업보건
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• s.80
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• pp.25-29
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• 1994

• 월간산업보건
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• s.79
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• pp.14-19
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• 1994