• Title/Summary/Keyword: 생물학적 제어

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Isolation of Bacillus spp. from Cheonggukjang and Its Antagonistic Effect against Bacillus cereus (청국장으로부터 Bacillus cereus에 대한 길항 균주 분리 및 길항 효과)

  • Lee, Nam-Keun;Park, Joung-Whan;Cho, Il-Jae;Kim, Byung-Yong;Kwon, Ki-Ok;Hahm, Young-Tae
    • Korean Journal of Food Science and Technology
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    • v.40 no.6
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    • pp.669-673
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    • 2008
  • For the development of a biological control method against B. cereus in cheonggukjang, 20 Bacillus spp. were isolated from the naturally fermented baektae and heuktae cheonggukjang, identified by using 16S rDNA sequences. Among the isolated strains, Bacillus sp. SC-8 was selected using the B. cereus lawn cell assay as an antagonistic microorganism against B. cereus. The culture medium of Bacillus sp. SC-8 after 24 hr of incubation at $37^{\circ}C$ also evidenced a high level of antagonistic activity. In cheonggukjang fermented with the mixed culture of Bacillus sp. SC-8 and B. cereus, antagonistic effect against B. cereus was maintained during the fermentation of cheonggukjang, while its effect was reduced during storage at $4^{\circ}C$ due to the decrement of cell population of Bacillus sp. SC-8. In Bacillus sp. SC-15, which was utilized a control, antagonistic activity against B. cereus was not demonstrated on the lawn cell plate assay and culture medium, but its effects were detected in cheonggukjang. Therefore, the production of antagonistic substances of Bacillus spp. depends on the fermentative environment.

Regulation of Copulation and Parturition in Black Rockfish, Sebastes Schlegeli by Photoperiod and Temperature (광주기와 수온에 의한 조피볼락, Sebastes schlegeli의 교미 및 출산 조절)

  • Park, Moo-Eog;Rho, Sum;Byun, Soo-Cheol;Lee, Chi-Hoon;Baek, Hea-Ja;Lee, Young-Don;Kim, Hyung-Bae
    • Journal of Aquaculture
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    • v.14 no.3
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    • pp.173-180
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    • 2001
  • Regulation of copulation and parturition of black rockfish, Sebastes schlegeli were induced by artificially controlled photoperiod and temperature. for the treated group, daylength of copulation and parturition period was maintained at 9L : 15D and 14L : l0D, respectively. Temperature was maintained at 12~13$^{\circ}C$ during the copulation period, and at 14~15$^{\circ}C$ during the parturition period. Gonadosomatic index (GSI) of male was low 0.12 from January to August and peak to 1.36~0.10 during November in the control group but 1.27 ~ 0.05 during October in treated group. GSI of female was about 1.82 from January to November 1996 and reached the maximum of 46.5 ~ 5.72 during April 1997 in the control group but 42.0~4.24 during february 1997 in the treated group. The pattern of ovary and testis development did not much differ between the treated and control groups. However, the copulation and parturition of black rockfish could be advance 2~3 months by controlling photoperiod and temperature.

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Evaluation of $^{14}C$ Behavior Characteristic in Reactor Coolant from Korean PWR NPP's (국내 경수로형 원자로 냉각재 중의 $^{14}C$ 거동 특성 평가)

  • Kang, Duk-Won;Yang, Yang-Hee;Park, Kyong-Rok
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.7 no.1
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    • pp.1-7
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    • 2009
  • This study has been focused on determining the chemical composition of $^{14}C$ - in terms of both organic and inorganic $^{14}C$ contents - in reactor coolant from 3 different PWR's reactor type. The purpose was to evaluate the characteristic of $^{14}C$ that can serve as a basis for reliable estimation of the environmental release at domestic PWR sites. $^{14}C$ is the most important nuclide in the inventory, since it contributes one of the main dose contributors in future release scenarios. The reason for this is its high mobility in the environment, biological availability and long half-life(5730yr). More recent studies - where a more detailed investigation of organic $^{14}C$ species believed to be formed in the coolant under reducing conditions have been made - show that the organic compounds not only are limited to hydrocarbons and CO. Possible organic compounds formed including formaldehyde, formic acid and acetic acid, etc. Under oxidizing conditions shows the oxidized carbon forms, possibly mainly carbon dioxide and bicarbonate forms. Measurements of organic and inorganic $^{14}C$ in various water systems were also performed. The $^{14}C$ inventory in the reactor water was found to be 3.1 GBq/kg in PWR of which less than 10% was in inorganic form. Generally, the $^{14}C$ activity in the water was divided equally between the gas- and water- phase. Even though organic $^{14}C$ compound shows that dominant species during the reactor operation, But during the releasing of $^{14}C$ from the plant stack, chemical forms of $^{14}C$ shows the different composition due to the operation conditions such as temperature, pH, volume control tank venting and shut down chemistry.

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Treatment Outcome and Analysis of the Prognostic Factors of High Grade Glioma Treated by Postoperative Radiotherapy (고악성도 신경아교종의 수술 후 방사선 치료 성적과 예후인자 분석)

  • Lee, Sun-Min;Park, Young-Je;Yoon, Won-Sup;Lee, Suk;Yang, Dae-Sik;Jeong, Yong-Gu;Kim, Chul-Yong
    • Radiation Oncology Journal
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    • v.28 no.3
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    • pp.117-124
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    • 2010
  • Purpose: To analyze the postoperative radiotherapy results and prognostic factors in patients with WHO grade 3 and 4 gliomas. Materials and Methods: A total of 99 patients with malignant gliomas who underwent postoperative radiotherapy between 1988 and 2007 were enrolled in this study. Total resections, subtotal resections ($\geq$50%), partial resections (<50%), and biopsies were performed in 16, 38, 22, and 23 patients, respectively. In total there were 32, 63 and 4 WHO grade 3, 4, and unspecified high grade gliomas, respectively. The biologically equivalent dose was in the range of 18.6 to $83.3\;Gy_{10}$ (median dose, $72.2\;Gy_{10}$). We retrospectively analyzed survival rate, patterns of failure, prognostic factors, and adverse effects. Results: The median follow-up time was 11 months and there were 54 patients (54.5%) with local failure. The one and 2-year survival rates were 56.6% and 29.3%, respectively, and the median survival duration was 13 months. The one and 2-year progression-free survival rates (PFS) were 31.3% and 18.2%, respectively, and the median PFS was 7 months. The prognostic factors for overall survival were age (p=0.0001), surgical extents (subtotal resection, p=0.023; partial resection, p=0.009; biopsy only, p=0.002), and enhancement of tumor in postoperative imaging study (p=0.049). The factors affecting PFS were age (p=0.036), tumor enhancement of the postoperative imaging study (p=0.006). There were 3 patients with grade 3 and 4 side effects during and after radiotherapy. Conclusions: In addition to age and surgical extents, tumor enhancement of the postoperative imaging study was included in the prognostic factors. The most common relapse patterns were local failures and hence, additional studies are needed to improve local control rates.

Results of Coventional Radiotherapy for Carcinomas of the Tonsillar Region (편도암의 방사선 치료 성적)

  • Nah Byung Sik;Nam Taek Keun;Ahn Sung Ja;Chung Woong Ki
    • Radiation Oncology Journal
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    • v.15 no.2
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    • pp.97-104
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    • 1997
  • of $PLC-\gamma$ 1 activity. Results : In the immunohistochemistry, the expression of $PLC-\beta$ was negative for all grnups. The expression of $PLC-\gamma$ 1 was highest in the group III followed by group II in the proliferative zone of mucosa. The expression of PKC-01 was strong1y positive in group I followed by group II in the damaged surface epithelium. The above findings were also confirmed in the immunoblotting study. In the irnrnunoblotting study, the expressions of $PLC-\beta,\;PLC-\gamma\;1,\;and\;PLC-\delta$ were the same as the results of immunohistochemistry The expression of ras oncoprotein was weakly Positive in groups II, III and IV. The of EGFR was the highest in the group II, III, followed by group W and the expression of PKC was weakly positive in the group II and III. Conclusion : $PLC-\gamma$ 1 mediated signal transduction including ras oncoprotein, EGFR, and PKC play a significant role irL mucosal regeneration after irradiation. $PLC-\delta$ 1 mediated signal transduction might have an important role in mucosal damage after irradiation. Further studies will be necessary to confirm the signal transduction mediating the $PLC-\delta$ 1.

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Overview of Real-time Visibility System for Food (Livestock Products) Transportation Systems on HACCP Application and Systematization (축산물 유통단계의 HACCP 적용과 체계화를 위한 실시간 관제시스템에 대한 현황)

  • Kim, Hyoun-Wook;Lee, Joo-Yeon;Hong, Wan-Soo;Hwang, Sun-Min;Lee, Victor;Rhim, Seong-Ryul;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.30 no.6
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    • pp.896-904
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    • 2010
  • HACCP is a scientific and systematic program that identifies specific hazards and gives measurements in order to control them and ensure the safety of foods. Transportation of livestock and its products is one of the vulnerable sectors regarding food safety in Korea, as meats are transported by truck in the form of a carcass or packaged meat in a box. HACCP application and its acceleration of distribution, in particular transportation, are regarded as important to providing consumers with ultimately safe livestock products. To achieve this goal, practical tools for HACCP application should be developed. Supply chain management (SCM) is a holistic and strategic approach to demand, operations, procurement, and logistics process management. SCM has been beneficially applied to several industries, notably in vehicle manufacture and the retail trade. HACCP-based real-time visibility system using wireless application (WAP) of the livestock distribution is centralized management system that enables control of temperature and HACCP management in real-time for livestock transportation. Therefore, the application of HACCP to livestock distribution (transportation, storage, and sale) can be activated. Using this system, HACCP management can be made easier, and distribution of safe livestock products can be achieved.