• The Korean Journal of Zoology
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• v.39 no.1
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• pp.36-46
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• 1996

An endogenous phenoloxidase (EPO) from earthworm, Lumbricus rubellus, has been purified and characterized. The purified EPO using ammonium sulfate fractionation, Blue-2, Phenyl-, and Q-sepharose chromatography steps was revealed in SDS-PAGE as a single protein banri with Mr. of 59 kl)a. A native strudure of the enzyme was examined with an in situ staining of a nondenatudng-PAGE using DL-dopa as a substrate. The result showed that a single band due to the EPO activity was located siighdy above a standard polypeptide with Mr. of 210 kl)a. These fads indicate that the EPO is an oligomeric enzyme. The presence of a monophenolase activity of the purified EPO, which hydroxylates tyrosine to dopa, was confirmed by observing dopachrome accumulation at 475 nm at PH 8.0 with a typical lag phase during 60 mm. of meausrement. A series of inhibition study has been performed for the enzyme with several divalent cation chelators such as phenyithiourea (Flu), 1, lO-phenanthroline, EDTA, and EGTA. Among them, only V'flj inhibited the enzyme with 1C0.5 of 65 MM, which indicated that copper was critical for the catalysis of EPO. The enzyme was maximally active at 35'C and pH 8.0 when L-dopa to dopachrome conversion was spectrophotometricaily monitored at 475 nm. The apparent Km values of P0 for L-opa were obtained as 1.86 mM and 13.8 mM at pH 6.5 and 8.0, respectively. The catalytic efficiencies at both pH were almost identical [(kat/Km)pH8.0/(kcat/Km)pH6.5 = O.92] while the Vmax at p11 8.0 was 6.6-fold higher than that at pH 6.5. This fact may indicate that pH affeds the catalysis at substrate and/or enzyme-substrate complex level rather than the enzyme itself. Taken together, the EPO was an oligomeric enzyme which did not require proteolysis for its activation. These results also indicated that the enzyme can exist, at least, in part as a latent form In vivo, which might be distinct from the prophenoloxidase activating system. Therefore, it is pertinent to consider that there must be certain regulatory molecules or phenomena in L. rubellus which make the 1,0 in a latent form in vivo before the foreign invasions.

• Korean Journal of Ichthyology
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• v.18 no.3
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• pp.223-233
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• 2006

The annual and spatial changes in the species composition of catch off Yeosu were analyzed using the daily sales slip catch data by a set net in the inshore waters off Dolsan Islands in Yeosu from April to October 2001, off Yeon Islands of Yeosu from April to October 2002 and in the offshore waters off Dolsan Islands of Yeosu from April to December 2003, respectively. Scomberomorus niphonius, Seriola spp., Trichiurus lepturus, Engraulis japonicus, Sarda orientalis, Todarodes pacificus, Pampus echinogaster, Sardinella zunasi, Scomber japonicus, Lophius litulon and Loligo beka were dominant species in abundance, indicating that pelagic fish were mainly caught by a set net off Yeosu. S. zunasi, P. echinogaster, Platycephalus indicus and L. beka inhabited mainly in the inshore waters, and S. niphonius, Seriola spp., T. lepturus, P. echinogaster, T. pacificus, Takifugu porphyreus and Pagrus major resided mainly in the offshore waters as the pelagic resident species. E. japonicus was a representative dominant species moving between the inshore and the offshore waters seasonally. S. zunasi and E. japonicus occurred in the inshore waters, and E. japonicus, L. litulon and Seriola spp. begain to be caught in the deep offshore waters in spring. Total catch was high during the summer season by migration of the open sea species such as T. lepturus, S. niphonius, S. japonicus, Seriola spp., S. orientalis, P. echinogaster and T. pacificus. In fall, S. niphonius, E. japonicus, Sphyraena pinguis, Siganus fuscescens and Leiognathus nuchalis were dominant in the inshore waters, and S. niphonius, P. echinogaster, Hyporhamphus sajori, S. japonicus and T. lepturus continued to occur from summer in the offshore waters but total catch decreased, indicating the typical seasonal variation pattern of the temperate region. Most of catchable fishes by a set net were the pelagic species showing a significant temporal variation. Collection and analysis of daily catch data by large set nets can be used to determine seasonal variation in species composition of pelagic fish in a study area.

• Tuberculosis and Respiratory Diseases
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• v.56 no.3
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• pp.261-268
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• 2004

Background : The resurgence of tuberculosis and outbreaks of multidrug resistant (MDR) tuberculosis have increased the emphasis for the development of new susceptibility testing of the Mycobacterium tuberculosis for the effective treatment and control of the disease. Conventional drug susceptibility testings, such as those using egg-based or agar-based media have some limits, such as the time required and difficulties in determining critical inhibitory concentrations, but these are still being used in many diagnostic laboratories because of no better lternatives, considering cost and accuracy. To overcome these limits, a rapid and simple method for new susceptibility testing, using live and dead assays, was applied for a bacterial cell viability assay to distinguish dead from live bacterial cells based on two-color fluorescence. Materials and Methods Strains : Forty strains were used in this study, 20 susceptible to all antituberculosis drugs and the other 20 resistant to the four first line antituberculosis drugs isoniazid, rifampicin, streptomycin and ethambutol. Antibiotics : The four antibiotics were dissolved in 7H9 broth to make the following solutions: $0.1{\mu}g\;isoniazid(INH)/m{\ell}$, $0.4{\mu}g\;rifampicin(RMP)/m{\ell}$, $4.0{\mu}g\;streptomycin(SM)/m{\ell}$ and $4.0{\mu}g\;ethambutol(EMB)/m{\ell}$. Results : Live and dead Mycobacterium tuberculosis cells fluoresced green and red with the acridin (Syto 9) and propidium treatments, respectively. These results are very well accorded with conventional drug susceptibility testing by proportional method on Lowensen-Jensen media (L-J) containing 4 drugs (INH, RMP, EMB and SM), showing a 93.7 % accordance rate in susceptible strains and 95% in resistant strains. Conclusion : The results of the drug susceptibility testing using the live and dead bacterial cell assay showed high accordance rates compared with the conventional proportion method on L-J. This finding suggests that the live and dead bacterial cell assay can be used as an alternative to conventional drug susceptibility testing for M. tuberculosis strains.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.1
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• pp.25-31
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• 2011

In this study, model-based $NH_4-N$ predictive control algorithm by using influent pattern was developed and evaluated for effective control application in $A^2/O$ process. A pilot-scale $A^2/O$process at S wastewater treatment plant in B city was selected. The behaviors of organic, nitrogen and phosphorous in the biological reactors were described by using the modified ASM3+Bio-P model. A one-dimensional double exponential function model was selected for modeling of the secondary settlers. The effluent $NH_4-N$ concentration on the next day was predicted according to model-based simulation by using influent pattern. After the objective effluent quality and simulation result were compared, the optimal operational condition which able to meet the objective effluent quality was deduced through repetitive simulation. Next the effluent $NH_4-N$ control schedule was generated by using the optimal operational condition and this control schedule on the next day was applied in pilot-scale $A^2/O$ process. DO concentration in aerobic reactor in predictive control algorithm was selected as the manipulated variable. Without control case and with control case were compared to confirm the control applicability and the study of the applied $NH_4-N$control schedule in summer and winter was performed to confirm the seasonal effect. In this result, the effluent $NH_4-N$concentration without control case was exceeded the objective effluent quality. However the effluent $NH_4-N$ concentration with control case was not exceeded the objective effluent quality both summer and winter season. As compared in case of without predictive control algorithm, in case of application of predictive control algorithm, the RPM of air blower was increased about 9.1%, however the effluent $NH_4-N$ concentration was decreased about 45.2%. Therefore it was concluded that the developed predictive control algorithm to the effluent $NH_4-N$ in this study was properly applied in a full-scale wastewater treatment process and was more efficient in aspect to stable effluent.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.130-135
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• 2010

To select the viable alternative substrates among the variable organic substrates for productivity enhancement and production cost-reduction of oyster mushroom in bottle culture, this study was carried out at mushroom research institute of GGRDA in 2007. In bottle culture of oyster mushroom (Plerutus ostreatus), the seedcakes of rape (RS), soybean (SS), coconut (CCS), and kapok (KS) were examined as substitute of cotton seedcake which was primary nutritive material of mushroom growing substrate. The chemical properties of substrate mixed with kapok seedcake is similar to the mixture with cotton seedcake in T-C, T-N, C/N ratio, and other nutrients. Mixed growing substrate containing cotton seedcake and kapok seedcake was superior to other mixtures 99.2% and 99.5%, respectively in spawning ratio and was faster mycellium growth in column test than that of soybeen seedcake, cotton + soybeen seedcake, and coconut seedcake. The period required in first pin-heading was 1-2 days longer in rape and soybeen seedcake mixture. Also there wad no primodia and fruitbody formation at soybeen seedcake mixture which had highest T-N content among the other mixed substrates. Yield per bottle and biological efficiency were highest of 144.6 g and 75.4%, respectively at kapok seedcake mixture. As a result, this study found that cotton seedcake can be replaced with kapok seedcake in bottle culture of oyster mushroom.

• Anatomy & Biological Anthropology
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• v.29 no.4
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• pp.145-154
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• 2016

The purpose of this study was to provide basic biometric data on Korean adults through magnetic resonance imaging (MRI)-based measurements of the distances between the apex of sacral hiatus (SH) and the termination of dural sac (DS), and between SH and conus medullaris (CM) because they are critical to the performance of epidural neuroplasty. A total of 200 patients(88 males and 112 females) with back pain, who had no spine fracture, significant spinal deformity, and spondyloisthesis were selected for this study. The subjects were of mean age 54.3 (20~84) years and mean height 161.3 cm (135~187). T2-weighted MRI images were used for correlation analysis to evaluate the relationships between the distances, and variables such as sex and height. In all patients, the mean distance between SH and DS was $62.8{\pm}9.4mm$ and the mean distance between SH and CM was $232.2{\pm}21.8mm$. The minimum distance and the maximum distance between SH and DS were 34.8 mm and 93.9 mm respectively, and the minimum distance and the maximum distance between SH and CM were 155.0 mm and 284.0 mm respectively. In female patients, both the distances between the SH and DS, and between SH and CM were shorter when compared to those of the male patients(p<0.05). Both the distances between SH and DS and between SH and CM showed a significant correlation with height(p<0.01). The results of this study will provide a useful biometric data on the distances between SH and DS and between SH and CM in Korean in ensuring clinical safety and in the development of more effective catheterization techniques for epidural neuroplasty in Korean.

• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.506-514
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• 2019

Unlike natural ecosystems, the urban ecosystem proVides an interdependent enVironment in which wild organisms and urban people co-exist. Hornets (genus Vespa) appearing in urban green and parks haVe a positiVe effect on urban ecosystems, but they also cause ecosystem disserVices that cause physical and psychological discomforts to the urban people. Children's parks, for example, are Very popular among children and residents for easy accessibility, and hornets also use them as bases and habitats. HoweVer, there is still a lack of spatial analysis of habitats and appearance characteristics of hornets in children's parks. This study installed hornet traps in 27 children's parks in Cheonan from April to NoVember 2018 in consideration of the life cycle of hornets. We captured a total of fiVe Vespa species (Vespa crabro, V. analis, V. mandarinia, V. ducalis, and V. Velutina) for 32 weeks and analyzed the emergence of hornets in relation to the composition of seasonal characteristics, species characteristics, and enVironmental spatial information. We captured a total of 818 hornets during the study period. They included 290 V. analis (35.4%), 260 V. crabro (31.8%), 100 V. ducalis (12.1%), 87 V. mandaninia (10.6%), and 81 V. Velutina(9.9%). Most of the hornets showed a common feature that queen hornets were largely captured in May through June after they awake from hibernation, and the number of caught hornets decreased sharply beginning in mid-June, which was the cooperatiVe period. HoweVer, V. Velutina showed a seasonal specificity that more than 80% were captured beginning in the third week of October when other hornet species had already entered a decline phase. The analysis of the number of hornets caught in each spot in children's parks showed significant difference among the spots as 363 hornets (44.3%) were captured in top children's parks, and 35 hornets (4%) were captured in bottom children's parks. In particular, the mean NDVI (Normalized difference Vegetation index) of the top six children's parks was 0.79, and that of the bottom six children's parks was 0.38 (t=2.67*, *=p<0.05), indicating a significant difference. The frequency of capturing hornets was high when the ground around the children's parks was grass or bare land. This study is meaningful as a reference study that confirms the ecological characteristics of hornets appearing in green and parks in the city. We expect it to be a foundation for effectiVe urban green area management in the future.

• Journal of Food Hygiene and Safety
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• v.36 no.6
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• pp.493-503
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• 2021

The study was designed to analyze raw and auxiliary materials of Korean traditional cookies such as Yugwa and Gangjeong, equipment and tools, personal hygiene of workers and microbial contamination of materials by each manufacturing process. In addition, it looked at washing method for reducing microorganisms at the site and reduction effect of microorganisms by frequency in the manufacturing processes of Yugwa. In the process of producing Korean traditional cookies, the level of total aerobic bacteria (TAB) in popped rice was 1.2 Log CFU/g and the level of TAB in finished products increased to 3.7 Log CFU/g. In the process of producing Yugwa, the level of TAB increased to a maximum of 6.5 Log CFU/g in the soaking process but decreased to 1.3 Log CFU/g in the frying process. However, the level of TAB increased again to 1.3 Log CFU/g in finished products that proves its recontamination. It is estimated that he manufacturing process causes cross-contamination that comes from the work tools, equipment or workers. In particular, the spatula, one of the work tools, was found to have 4.4 Log CFU/g of aerobic bacteria and 4.2 Log CFU/g of colon bacillus that show they are highly contaminated. In the soaking process of Yugwa that lasts seven days, the level of TAB was a maximum of 10 Log CFU/g and the level of total colon bacillus was 6.8 Log CFU/g. When compared with washing methods, using hands and tools or running water, it is confirmed that the level of both TAB and total colon bacillus decreased to 5.0 Log CFU/g and 2.8 Log CFU/g respectively when hands were washed with running water 10 times. The above result shows that it's required for workers to wash their hands as well as wash and disinfect work tools and equipment in the process of producing Korean traditional cookies at small-scale companies. In addition, to reduce the level of microbial contamination in finished products, workers are required to apply their reduction method at the site.

• Journal of the Korean Wood Science and Technology
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• v.32 no.4
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• pp.8-17
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• 2004

Antifungal, antibacterial, and antioxidative activities of ethanol extracts from 65 families 263 species were investigated to select tree species for the utilization of natural fungicide or preservative resources. The antifungal activities of extracts from wood, leaf and bark were measured as hyphal growth inhibition rate using four plant pathogenic and five wood rotting fungi. High inhibitory effect on the fungi growth was found in five species of Pinaceae (Pinus koraiensis, P. rigida, P. densiflora, P. banksiana. Cedrus deodara), three species of Cupressaceae (Juniperus rigida, J. chinensis, Chamaecyparis obtusa) and three species of Leguminosae (Albizzia julibrisssin, Sophora japonica, Maackia amurensis), respectively. Antibacterial activities of ethanol extracts were determined by means of disc-agar plate diffusion method using three gram-positive and five gram-negative bacteria. The ethanol extracts, which showed prominent effect on the suppression of bacteria growth, were six species of Betulaceae (Carpinus tschonoskii, C. coreana, C. laxiflora, Alnus hirsuta, A. firma, Betula schmidtii), five species of Fagaceae (Castanopsis cuspidata var. sieboldii, Quercus serrata, Q. mongolica, Q aliena, C crenata), four species of Euphorbiaceae (Aleurites fordii, Sapium sebiferum, S japonicum Mallotus japonicus) and three species of Elaeagnaceae (Elaeagnus umbellata, Elaeagnus glanbra, Elaeagnus macrophylla). According to these results, the extracts from Zelkova serrata, Pinus densiflora, Maackia amurensis, Chamaecyparis obtusa and Juniperus chinensis could be available for natural fungicide or food preservatives, because ethanol extracts from these species indicated excellent antifungal and antibacterial activities. In order to test antioxidative activities of ethanol extracts, free radical scavenging method was adopted with 1,1-diphenyl-2-picrylhydrohydrazyl (DPPH). Free radical scavenging activity was proved very high in the extracts of eight species of Rosaceae (Eriobotrya japonica, Prunus takesimensis, P yedoensis, P padus, P armeniaca var. ansu, Chaenomeles sinensis, Stephanandra incisa, Rosa multiflora) and five species of Ericaceae (Rhododenron mucronulatum, R. scblippenbacbii, R. yedoense var. poukhanense, Vaccinium bracteatum, V oldbami), resvectively. It turned out from this study that only six species among 48 species of Rosaceae showed less than 80% free radical scavenging activity. As a consequences, it could be deduced that the components effective on antioxidative activity commonly exist in Rosaceae plant family.

• Journal of Bio-Environment Control
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• v.21 no.3
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• pp.192-198
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• 2012

Experiments were conducted to investigate the optimum concentration of nutrient solution in hydroponics for strawberry 'Ssanta' bred at Gyongsangbuk-do Agricultural Research & Extension Services. Nutrient solutions for strawberry, which made by Yamazaki, were supplied EC (Electrical Conductivity) 0.6, 0.8, 1.2, and $1.8dS{\cdot}m^{-1}$ after planting on cocopeat medium during experiment period. Growth of shoot of strawberries did not show statistical differences among treatments. Fruit length showed the longest in EC $0.8dS{\cdot}m^{-1}$ in all clusters. In the second flower cluster, fruit length showed longer in EC 0.8 and $1.2dS{\cdot}m^{-1}$ than EC 0.6 and $1.8dS{\cdot}m^{-1}$. In the third flower cluster, it showed the longest in EC 0.8 and $1.2dS{\cdot}m^{-1}$, followed by 0.6 and $1.8dS{\cdot}m^{-1}$. The longest was in EC $0.8dS{\cdot}m^{-1}$ and the shortest in EC $1.8dS{\cdot}m^{-1}$ in the fourth flower cluster. Fruit diameter did not show significant differences among treatments, but longest in EC 0.8 and $1.2dS{\cdot}m^{-1}$ in all clusters. The heaviest mean fruit weight appeared in EC $0.8dS{\cdot}m^{-1}$ in all flower clusters. And heavier in EC $1.2dS{\cdot}m^{-1}$ in the second and third clusters. Also the weight was significantly light in plants grown in EC 0.6 and $1.8dS{\cdot}m^{-1}$ in the second and third cluster. Soluble solids of fruit was the highest in EC $0.6dS{\cdot}m^{-1}$ in all clusters. As the results, we came to the conclusion that the optimum EC for strawberry 'Ssanta' was EC $0.8{\sim}1.2dS{\cdot}m^{-1}$ in this experiment.