• The Korean Society of Law and Medicine
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• v.2 no.2
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• pp.107-123
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• 2001

• The Korean Society of Law and Medicine
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• v.6 no.1
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• pp.395-415
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• 2005

• The Journal of the Convergence on Culture Technology
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• v.5 no.4
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• pp.171-181
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• 2019

In this article, we examined designer babies and bioethics, and we reviewed how Anna argued that she had the rights to her body through trial with parents. No matter what purpose a cloned human or designer baby was born with, it is worth being respected as the birth itself. After learning the secrets of his birth in Jodie Picoult's My Sister's Keeper, which is based on a designer baby, Anna falls into confusion of identity and sues parents who decide to transplant organs regardless of Anna's will. Anna argues that she has the right to make decisions about her own body and that even her parents can't disregard her opinions. Scientists should stop their research if using biotechnology to reproduce humans, such as designer babies and cloned humans, can destroy the natural order.

• Journal of Life Science
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• v.3 no.4
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• pp.194-208
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• 1993

DNA 복제시 중추적 단백질은 DNA 합성을 수행하는 DNA polymerase이다. 따라서 DNA polymerase의 구조 및 기능에 대한 연구는 DNA polymerase의 중합반응에 대한 기작을 비롯하여 교정 및 수선기능에 대한 정보를 얻게 함으로써 복잡한 DNA 복제 기적을 이해하는 첩경이 된다. Bacteriophage T7의 Gene 5 단백질은 T7 DNA polymerase로 Richardson group에 의해 처음으로 발견되었으며, E. coli의 12 KDa thioredoxin과 tight complex를 형성한다. T7 DNA polymerase의 클로닝은 분자생물학의 새로운 장을 열어준 중요한 의미를 지닌다 . 본 연구에서는 T7 DNA polymerase의 구조적, 기능적 특성을 파악하고 DNA 염기서열 분석에의 응용 및 DNA 염기서열 결정을 위한 새로운 전략 및 최근연구 동향에 대해 기술하였다.

• Microbiology and Biotechnology Letters
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• v.19 no.3
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• pp.215-221
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• 1991

An autonomous replication sequence (ARS) derived from Cephalosporium acremonium ATCC 20339 was cloned in Sarchuromyces cerevisiae SHY 3 using YIp5 as a cloning vector. A new recombinant plasmid, designated pCY-2, which contained a 3.7 kb BamHI fragment of C. acrenzonium DNA showed the highest stability among the 40 recombinant plasmids composed of the YIp5 2nd ARS of C. ucremoniztm. Also, Southern hybridization and transformation of E, cull with DNA purified from yeast transformants verified that pCY-2 autonomously replicates in yeasts. Transformation efficiency and plasmid stability of pCY-2 in yeast were higher than those ol YRp 7 containing ARS which originated from yeast. Detailed studies by subcloning revealed that two ARSs existed within 2.6 kb of the insert, which is a novel discovery. However, it was concluded that these two ARSs were ligated during the gene manipulation in vitro.

• Journal of Life Science
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• v.20 no.5
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• pp.789-793
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• 2010

Artificial chromosome manipulation and amplification of single-copy yeast artificial chromosome (YAC) are usually required in order to use YACs for applications such as physical mapping and functional analysis in eukaryotes. We designed and implemented a Simultaneous YAC Manipulation-Amplification (SYMA) system that combines the copy number amplification system of YAC with a convenient YAC manipulation system. To achieve the desired split and to amplify a YAC clone-harboring plant chromosome, a pBGTK plasmid containing a conditional centromere and thymidine kinase (TK) gene was constructed as a template to amplify the splitting fragment via PCR. By splitting, new 490-kb and 100-kb split YACs containing the elements for copy number amplification were simultaneously generated from a 590-kb YAC clone. The 100-kb split YAC was then successfully amplified 14.4-fold by adding 3 mg/ml sulfanilamide and $50\;{\mu}g/ml$ methotrexate (S3/M50) as inducing substances.

• Journal of Life Science
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• v.33 no.11
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• pp.950-955
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• 2023

Cell division is an essential process for the survival and development of living organisms. It is critical that duplicated chromosomes are properly segregated into daughter cells during mitosis. The centrosome is the core organelle that forms the microtubule-organizing center (MTOC), which generates the microtubules that make up the mitotic spindle during cell division. The centrosome is also involved in cell signaling and motility. In normal cells, there is one centrosome in G1 that replicates into two in the S phase and matures through G2. During the M phase, duplicated centrosomes move to both ends of the cell, and spindle microtubules that are generated from MTOC move the chromosome to both ends. The cells then split into two to complete the cell division. However, a phenomenon called centrosome amplification (CA), in which the number of centrosomes is higher than normal, is common in cancer cells and can lead to chromosome instability (CIN). This paper discusses the process of centrosome replication and the role of PLK4 in this process. The possible consequences of centrosome amplification and how the PLK4 inhibitor may be able to treat certain types of cancer cells, such as breast cancer and neuroblastoma, will also be discussed.

• The Science & Technology
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• v.32 no.1 s.356
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• pp.14-15
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• 1999

97년 2월 복제양 '돌리'의 탄생이 전세계를 경악시킨 이후 지난해 11월엔 인간의 세포핵을 소의 난자에 이식시켜 배양하는데 성공함으로써 반인반수의 시대가 열렸다. 최근 생명공학기술개발에 따른 안전과 윤리를 문제삼는 환경단체ㆍ소비자단체ㆍ종교단체들이 반대운동에 앞장서고 있다. 그러나 과학기술계에선 이같은 반대가 생명공학에 대한 무지나 오해에서 비롯된 것이라고 일축하고 있다. 이러한 논쟁을 조정하고 과학기술정책을 결정할 때는 시민을 대화의 상대와 의사결정의 주체로 하는 '합의회의'방식을 도입하는 것이 바람직하다.

• The Microorganisms and Industry
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• v.17 no.2
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• pp.18-21
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• 1991

생물은 자기 복제를 통한 생장이나 생명유지를 위해 에너지를 필요로 한다. 화학영양생물은 화학에너지를 발효 혹은 호흡을 통해 생물학적 에너지로 전환시키며, 광영양생물은 광합성 작용을 통해 광에너지를 이용한다. 발효, 호흡, 광합성은 모두 산화-환원 반응을 통해 이루어진다. 생물의 모든 에너지 전환반응은 산화-환원 반응, 즉 전자의 흐름으로 이루어지며 생명현상이 에너지를 필요로 하기 때문에 생명현상은 전자의 흐름으로 이루어진다고 할 수 있다. 모든 생물이 에너지 전환 반응에 산화-환원 반응을 이용한다는 말은 생물이 많은 종류의 산화-환원 효소를 보유하고 있다는 뜻이며, 실제 많은 종류의 산화-환원 효소가 발견되고 연구되었다.

• Journal of Science and Technology Studies
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• v.1 no.1 s.1
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• pp.71-103
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• 2001

Usually social control and acceptance of science and technology is thought to be a separate or even confronting thing. But this paper aims to go beyond this simple and dichotomous thinking. Rather, this paper argues that social control and acceptance of science and technology can be combined altogether organically. In other words, this paper argues that social control of a particular science and technology has functional effects to the acceptance of that science and technology. Social control of a particular science and technology is a signal that something is going wrong; it reallocates attention and enhances social awareness; it evaluates ongoing activity; and it alters this activity in various ways to secure a sustainable future. In order to prove this functional hypothesis, this paper tries a case study of biotechnology from a perspective of sociology of science and technology. Especially social controversies around genetically modified organisms(GMOs) and embryo cloning in Korea are analysed deeply. As a conclusion, this paper proposes some policy implications of this study.