• Title/Summary/Keyword: 색소 생성

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Growth and Pigment Formation of Genus Monascus on Medium Compositions (배지에 따른 Genus Monascus의 증식도와 색소함량)

  • 윤은경;김미정;김순동
    • Food Science and Preservation
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    • v.9 no.4
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    • pp.425-428
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    • 2002
  • Growth and pigment formation in Genus Monascus(M. pilosus and M purpureus) related 15 kinds of culture media(Lin medium, SP medium, YM medium, YE medium, GMIN medium, SMO medium, MY medium, GY medium, Nishikawa medium, sucrose medium, stock culture, Mizutani medium, modified Lin medium, Toya medium and rice medium) were investigated. Mizutani medium and Lin medium among 15 kinds of the culture media showed good growth for M. pilosus, M purpureus, fresh mycelium weight cultivated for 10 days at 30$^{\circ}C$ was each 24.5∼26.9 or 15.9∼17.2 g/100 mL. The culture media which showed higher content of pigment production in two fungi were Lin medium(OD: 1.2 ∼ 1.6 and Mizutani medium(OD: 0.8 ∼ 1.0) that showed higher in M. pilosus.

Stability of the Pigments from Monascus purpuresu CBS 281.34 (Monascus purpureus CBS 281.34가 생성하는 홍국 색소의 안정성)

  • 임성일;곽은정
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.4
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    • pp.711-715
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    • 2004
  • The stability of 80% ethanol-soluble pigments from Monasus purpureus CBS 281.34 was investigated according to storage temperature, pH and addition of organic acid. Also, the stability of ethanol-soluble pigment in aqueous system was examined after the addition of distilled water in the range of 0∼80% during the storage at 1$0^{\circ}C$ and 2$0^{\circ}C$ for 4 weeks with water soluble pigment. The heat stability was the highest (9.74%) when the 80% ethanol-soluble pigments were stored at 1$0^{\circ}C$ for 4 weeks. However, the 80% ethanol-soluble pigments stored at 6$0^{\circ}C$ and 8$0^{\circ}C$ for 24 h and 12 h greatly decreased by 23.06% and 30.36%, respectively. Although the 80% ethanol-soluble pigments were stable in the range of pH 4∼8, the degradation rate of pigment increased at pH 2 and PH 10.80% ethanol extract was adjusted to PH 4 by adding organic acids. The rate of pigment degradation was not different from control for 4 weeks. Red pigment was stable in the treatment of organic acids. And the stability of ethanol-soluble pigment in aqueous system was gradually decreased as the pigment content and storage time increased. Additionally, the stability of ethanol-soluble pigment was higher at 1$0^{\circ}C$ than at 2$0^{\circ}C$.

Induction of lipid peroxidation and melanoma cell death by turmeric oleoresin through its photosensitizing properties (심황색소의 감광활성과 빛 조사에 의한 지질산화 및 세포독성 유도 효과)

  • Kim, Hee Jeong;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.54 no.1
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    • pp.59-65
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    • 2022
  • Curcuminoids in turmeric oleoresin (TO) are known to be effective antioxidants; they exhibit photosensitizing properties under light. In this study, the photoreactive properties of TO and its consequent induction of lipid peroxidation and cytotoxicity were evaluated. TO exhibited photosensitizing activities as evidenced by the reduction of nitro blue tetrazolium and by the decolorization of formazan under light, whereas light-irradiated TO did not enhance the levels of reactive oxygen species. The levels of hydroperoxide and thiobarbituric acid reactive substance (TBARS) were significantly elevated under a fluorescent light (10 W/m2) in corn, canola, and soybean oils containing 10-40 and 20-80 ㎍/mL of TO (p<0.05) but not in olive oil. Canola oil was the most sensitive to photo-oxidation induced by TO. The level of TBARS from linoleic acid in the oil in water system was, however, decreased by TO under light. The cytotoxicity effect of TO on melanoma cells was also substantially enhanced under light.

Antioxidative and Anti-inflammatory Activity of Extract from Milling By-products of Sorghum Cultivar, 'Hwanggeumchal' (황금찰수수 도정부산물의 항산화 및 항염증 활성)

  • Ra, Ji-Eun;Park, Ji-Young;Seo, Woo Duck;Sim, Eun-Yeong;Ko, Jee Yeon;Nam, Min-Hee;Chung, Ill-Min;Han, Sang-Ik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.4
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    • pp.463-469
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    • 2014
  • Sorghum has been consumed as one of the important staple food in the semiarid tropics of Africa and Asia. Sorghum is rich in starch, protein, essential vitamins and minerals and grows relatively well in dry climate regions when it compared with other staple food crops. Sorghum has taken an increased interest due to several studies that report about the beneficial effects of sorghum on human health. In the present study, we investigated the antioxidative and activity of extract of milling by-products (hull and bran) of Korean sorghum cultivar, 'Hwanggeaumchal' as well as its grain. Hull extract showed the highest total polyphenol contents ($29.7{\pm}0.2mg\;GAE/100g$) and major four pigments content ($322.6{\pm}14.5mg/100g$). From results of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging activity, hull extract ($IC_{50}$, $6.3{\pm}0.1{\mu}g/mL$) was also showed the strongest antioxidative effects. Bran and grain showed similar polyphenol, pigments contents and antioxidative effects. We determined cell viability by MTT assay and evaluated the anti-inflammatory activity by measuring nitric oxide (NO) of hull, bran and grain methanol extract (0.5% HCl v/v) on RAW 264.7 cells. Hull extract treatment was significantly decreased NO production with dose-dependant manner. Apigeninidin as one of the major pigment of hull was showed inhibitory activity against NO production without cytotoxicitiy. Therefore, sorghum milling by-products can be used as a good source of antioxidative and anti-inflammatory agents.

Studies on the Production of Carotenoid Pigments by Microorganism [Part I] - The Carotenoid Production in Rhodotorula glutinis var. glutinis Sw-17 - (미생물(微生物)에 의(依)한 Carotenoid색소(色素) 생성(生成)에 관(關)한 연구(硏究) [제1보(第1報)] - Rhodotorula glutinis var. glutinis sw-17의 Carotenid생성(生成)에 관(關)하여 -)

  • Park, Ki-Hyun;Park, Sung-Oh
    • Applied Biological Chemistry
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    • v.19 no.4
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    • pp.243-247
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    • 1976
  • A potent intracellular-lipid-producing yeast, Rhodotrorular glutinis var. glutinis sw-17 was studied on the production of carotenoids after shaking the cultures for 8 days at $25^{\circ}C$. The pigments were extracted with solvents and chromatographed with columns for its isolation. The carotonoids were identified by their positions on the column, and by their light-absorption curves. Close agreement was obtained between the absorption maxima of the isolated pigments and published literature values. The characteristic wave length maxima and the extinction values used for quantitative determination. The caroteniod pigments produced by the yeast were composed of torularhodin(28.52%), torulene(38.16%), neurosporene(1.49%), ${\gamma}-carotene(9.88%)$, ${\beta}$-zeacarotene(2.0%), ${\beta}-carotene(19.95%)$ and ${\delta}-carotene(trace)$.

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Anti-photoaging Effects of Fermented Soybean (Bio-Peptone®) (대두 발효물(Bio-Peptone®)의 광노화 억제 효과)

  • Kim, Eun Ju;Shim, Myeong Kuk;Jeong, A Ram;Kim, Ae Jung
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.45 no.1
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    • pp.27-36
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    • 2019
  • Soybean (Glycine max), as one of the foods with high plant proteins, contains a large amount of bioactive compounds and known to be effective in cardiovascular disease and obesity as well as in improving skin condition. The purpose of this study was to investigate the anti-photoaging effects of soybean fermented with Lactobacillus Rhamnosus ($Bio-Peptone^{(R)}$) by assessment of cytotoxicity against UVB, collagen synthesis after UVB-irradiation, tyrosinase activity, and melanin synthesis. Results showed that $Bio-Peptone^{(R)}$ protected skin fibroblasts against UVB-induced cytotoxicity and increased type I collagen synthesis. Furthermore, $Bio-Peptone^{(R)}$ significantly inhibited tyrosinase activity and reduced melanin contents. This study suggests that $Bio-Peptone^{(R)}$ has protective effects against UVB-induced skin damage. Thus, it is concluded that $Bio-Peptone^{(R)}$ is able to prevent skin damage against UVB and thus acts as anti-photoaging materials by protecting skin forming wrinkles and skin pigments.

Anthocyanin Production from Culture of Alnus hirsuta Callus (물오리나무 캘러스로부터 Anthocyanin 생산)

  • 안정선
    • Journal of Plant Biology
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    • v.37 no.2
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    • pp.237-244
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    • 1994
  • Callus tissues derived from protoplast of Alnus hirsuta were shown to produce red pigments under the continuous light. The R4 cell line with high yield of the pigments was selected. One of the red pigments was identified as anthocyanin; glucose and galactose as sugar moiety and cyandin as aglycone, by spectroscopic analysis, cellulose TLC and GLC. Both callus growth and anthocyanin accumulation were remarkably stimulated by 2,4-D. The highest anthocyanin yield was observed when $1\;\mu\textrm{M}$ 2,4-D in combination with $0.1\;\mu\textrm{M}$ BAP was supplemented to the culture medium. In case of inorganic salts, anthocyanin yield was enhenced by reducing concentration of phosphate from 5.0 mM to 2.5 mM and by increasing the ratio of ${NO_3}^{-}$ type to 80% for total nitrogen source.source.

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Changes in chemical properties and cytotoxicity of turmeric pigments by microwave treatment (마이크로파처리에 의한 심황색소의 화학안정성 및 세포독성 변화)

  • Song, EiSeul;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.49 no.6
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    • pp.693-698
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    • 2017
  • Turmeric is a yellow food-coloring spice containing curcuminoids, curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BMC), which have several physiological effects. In the present study, the effect of microwave irradiation on the chemical properties, antioxidant activity, and cytotoxicity of turmeric were investigated. Degradation of turmeric pigments was accelerated upon increase in irradiation time or intensity at 405 nm. Residual levels of curcumin, DMC, and BMC after 5 minutes of irradiation at 700 W were 11.3, 34.4, and 71.2%, respectively. Scavenging activities of turmeric pigment against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH) peroxyl radical and nitrite were enhanced significantly after microwave radiation. However, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity remained unaffected. Cytotoxic activity of turmeric was significantly reduced, and hydrogen peroxide generated from turmeric increased after microwave irradiation. The results obtained indicate that microwave irradiation affects chemical stability and bioactivity of turmeric pigment. Hence, these effects should be considered when processing foods containing turmeric pigments.