• Proceedings of the Korea Information Processing Society Conference
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• 2017.04a
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• pp.848-849
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• 2017

차세대 염기 분석(NGS) 장비의 발달로 시퀀스 분석에 대한 연구는 가속화 되고 있다. 조각들로 이루어진 리드들을 어셈블하는 방법부터 이미 유전체의 정보가 알려진 데이터베이스를 이용하여 정보를 명명하는 방법까지 다양한 방법들에 대한 방법들이 주를 이루고 있다. 하지만 어셈블하는 툴마다 다른 입력 포맷을 요구하고 있어 NGS의 결과로 다양한 방법으로 어셈블하여 비교 분석하기 쉽지 않다. 뿐만 아니라 생물 학자들이 세포내의 진화나 계통발생학적 분류를 위한 연구를 위해서 유전체 지도 완성 후 세포내의 기관별 분리 분석이 필요하나 참고 시퀀스로부터 매핑 및 기관별 분리 분석을 위해 사용목적에 따라 입력 포맷이 다른 다른 툴을 사용해야한다. 따라서 본 논문에서는 핵, 색소체, 미토콘드리아와 같은 세포내 기관에 대한 정보를 알기 위해 최소의 정보를 입력하여 분석하고자하는 시퀀스을 입력하여, 최대한 유사하게 매칭되는 유전체를 찾아 분석하는 방법을 제안함으로써, 진핵세포내의 발생학적 연구에 도움이 되는 방법을 제안하고자 한다.

• The Korean Journal of Mycology
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• v.24 no.4 s.79
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• pp.237-245
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• 1996

Esterase isozyme band patterns were compared between the wild strain and commercial strain of Flammulina velutipes. Monospores were isolated from wild strain. ASI4019 and their mating types were determined. We investigated the relationship between pigmentation on the plate and fruitbody color to understand genetic relationship among F. velutipes strains. Dikaryotic strains mated between nonpigmenting strains produced white fruitbodies. However dikaryon obtained from mating between nonpigmenting monokaryon and brown pigmenting monokaryon produced brown fruitbody as the dikaryon obtained from mating of brown pigmenting monokaryons. The white fruitbody from wild strain was distinguished from that of commercial strain. When the nonpigmenting wild monokaryon was mated with commercial monokaryon, pale brown mushroom was produced. The BC1F1 was obtained by mating the above mentioned $F_1$ with commercial monokaryon. Fruitbody color of BC1F1 shared two types; one strain with all pale brown fruitbodies, and the other strain with separated eight pale brown and two mixed type involving pale brown and white fruitbodies.

• The Korean Journal of Zoology
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• v.9 no.1
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• pp.39-45
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• 1966

백서(Rattus horvegicus var. albinus) 50 두를 키우며 자성백서의 발정을 질정도말법으로 확인하여 임신케 한후 각 단계별로 80개의 배를 얻었다. 고정액은 Bouin's solution 과 Carnoy's solution II였으며 표본은 5-7$\mu$의 두께로 연속절편을 만들었다. 염색은 Delafield's hematoxylin 과 eosin-Y의 이중염색법과 신경섬유를 관찰하기 위하여 Ramoney Cajal's silver-nitrate method를 사용하였다. 실험결과는 안와가 stage 17에서 나타났고 안포가 stage 18에서 형성되었다. 심안포가 stage 19에서 형성되었고 망막판과 수정체판이 stage 21에서 형성되었으며 체표외배엽과 접촉하고 있는 안포의 부분은 stage 22(A)에서 안배를 형성하기 위해 몰입되었다. state 22 (B)에서 수정체공은 아직 열려 있었고 stage 23(A)에서 색소립이 망막의 색소층에 나타났고 수정체공이 닫혀졌다. 수정체와는 stage 23(B)에서 체표외배엽으로부터 분리되었고 수정체 내벽세포의 신장이 stage23(C)에서 시작되었다. 수정체강은 stage 25에서 수정체섬유에 의해서 인멸되었고 stage 26에서 홍채가 형성되기 시작하였다.

• Journal of the Korean Graphic Arts Communication Society
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• v.12 no.1
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• pp.29-42
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• 1994

• Applied Biological Chemistry
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• v.46 no.1
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• pp.16-22
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• 2003

Interrelation between the antioxidative activities of 70% ethanol extracts from bran fraction of 23 kinds of colored rice and chuchung as a control were examined. Antioxidative activities were evaluated by assaying reducing power, electron-donation ability to DPPH free radical, scavenging activity of hydroxy radical $({\cdot}OH)$ generated through Fenton reaction and inhibitory activity on lipid peroxidation using linoleic acid autoxidation system, respectively. Among 24 varieties of colored rice LK 1-3-6-12-1-1 had the strongest reducing power followed by Elwee, DZ 78, Jumlalocal-1 and SC-45 in decreasing order. The electron-donating ability to DPPH radical was higher in order of HP 883-1-1-1-B-1-1, HP 833-1-3-1-1-1, LK 2-7-12-1-1 and DZ 78. The hydroxy radical scavenging activity was higher in order of DK-1, IR 1544-38-2-2-1-2-2, SC-5 and SC-45 but LK 2-7-12-1-1 had oxidative effect. In the liaoleic acid autoxidation model system, RGS No 336, LK 1B-2-1-1, LK 1B-4-12-1-1, LK 1A-2-12-1-1, LK 2-7-12-1-1 and HP 883-1-1-1-B-1-1 exhibited strong antioxidative activities but Elwee, Jumlalocal-l and SC-45 showed to have oxidative effects. The rice variety of highest pigment content was Elwee and the next were RGS-No 336, IR 1544-38-2-2-1-2-2 and SC-5 with the order of higher content. The reducing power was correlated with the quantity of the pigment in the ethanolic extract of rice bran and SC-5 showed relatively high antioxidative activity in every results of antioxidative activity tests.

• Journal of the Korean Chemical Society
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• v.40 no.3
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• pp.209-215
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• 1996

Fluorane dyes has been widely used for pressure and heat sensitive recording system as color forming materials. A new method of synthesizing fluorane dye by the Bamberger reaction has been developed. Electorchromic properties of fluorane dyes was discussed. The fading reaction kinetics follow a first-order relationship and resolved in two kinetics of first-order reaction with rate constant $k_1$ and $k_2$.

• Korean Journal of Plant Tissue Culture
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• v.25 no.6
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• pp.487-487
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• 1998

• Journal of the Korean Graphic Arts Communication Society
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• v.18 no.2
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• pp.125-132
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• 2000

To take an information of substitutent effect and thermodynamic of association at thiacarbocyanines, association constant $K_{D}$, free energy of association $\Delta$ $G_{D}$, enthalpies of association $\Delta$ $H_{D}$ were measured by spectrophotometer according to dependence on concentration of dyes and temperature of aqueous MeOH system. With increase of concentration dyes or decrease of temperature of aqueous MeOH system, absorption spectra of dyes exhibited equlilibria shift toward the dimer. Phenyl meso substituted thiacarbicyanine, DyeIII, resulted in higher $K_{D}$ and $\Delta$ $G_{D}$ values than DyeI and DyeII. To easily make an association of thacarbicyanine, meso substutituent group need to be a flat structure, which will make a park as well as lain tiles.tiles.ructure, which will make a park as well as lain tiles.les.

• KSBB Journal
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• v.22 no.5
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• pp.271-277
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• 2007

Natural pigments have many applications like colouring agent, pigments, food additives, and antiseptics. At present, instead of synthetic pigments that have contributed to the development of industry, many kinds of natural pigments have been developed. The constituents of gardenia fruits, Gardenia jasminoides ELLIS, are traditionally known as herb medicine and natural dyes/pigments due to the customer is needs. The fruits produce yellow carotenoid pigments and iridoid compounds. The two main components in the yellow pigments are called crocin and crocetin. The extraction mode of yellow pigment from Gardenia is depended upon the extraction time, temperature, and volume of solvent. Red pigments or blue pigments formed from geniposide and amino acids have been reported a lot. Geniposide, the principal iridoid glucoside contained in gardenia fruit, was hydrolyzed to genipinic acid or genipin as a precursor for the pigment by enzymatic or chemical reaction. These red or blue pigments prepared with materials hydrolyzed of geniposide and amino acid and had properties governed by the electrostatic character of the amino acid. The pigments showed good stability to heat and pH but were gradually bleached by light while the other natural pigments are unstable in light, heat, acid, and base solution. The safety of the pigments was considered to be of little virulences in comparison to synthetic pigments.

• Journal of Life Science
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• v.20 no.10
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• pp.1563-1568
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• 2010

In the course of a research concerning the molecular mechanism of hypocotyl elongation that occurs during soybean seedling growth in darkness, we have generated a number of ESTs from a cDNA library prepared from the hypocotyls of dark-grown soybean seedlings. Comparison of the ESTs assigned a cDNA clone as a putative plastidic ATP-binding-cassette (ABC) protein homologue. The soybean GmNAP1 protein contains an N-terminal transit peptide which targets it into the chloroplast. The transcription level of the GmNAP1 gene was investigated under continuous red light, continuous far-red light, and complete darkness. The main function of this NAP1 protein is the transport of protoporphyrin IX which is the precursor of chlorophyll from the cytoplasm to the chloroplast. The GmNAP1 gene was transferred into the Arabidopsis under the CaMV 35S promoter. The chlorophyll level of this transgenic Arabidopsis plant was much higher than the chlorophyll level of the wild type Arabidopsis plant.