• Korean journal of applied entomology
• /
• v.24 no.4 s.65
• /
• pp.223-230
• /
• 1986

A series of experiments were carried out to evaluate the differences in susceptibility of the 13-aphidicidial insecticides in the green peach aphids(Myzus persicae) collected from 13 different localities. The aphids were reared and increased on the potted tobacco plants in the laboratory. The susceptibility to insecticides was compared at the $LC_{50}$ levels with the resistant ratio by a leaf-dip method with a laboratory strain reared for 2 years in the laboratory without exposure to insecticides. The susceptibility to the insecticides was greatly varied with the local strains. The local strains demonstrated relatively high resistance to the insecticides over the laboratory strain at the $LC_{50}$ level; in resistant fold, 2.3 to 519.0 to cypermenthrin, 2.3 to 494.5 to decamethrin, 2.8 to 442.4 to fenvalerate, 2.5 to 170.6 to formothion, 1.5 to 494.5 to decamethrin, 2.8 to 442.4 to fenvalerate, 2.5 to 170.6 to formothion, 1.5 to 231.8 to phosphamidon, 3.1 to 42.1 to monocrotophos, 1.0 to 30.9 to phenthoate+dimethoate, 1.8 to 21.0 to heptanophos, 2.1 to 24.8 to oxydemeton-methyl, 1.0 to 24.9 to thiometon, 1.6 to 4.7 acephate, 0.8 to 4.1 to pirimicarb.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
• /
• v.10 no.5 s.92
• /
• pp.61-67
• /
• 1989

• Korean journal of applied entomology
• /
• v.32 no.3
• /
• pp.265-270
• /
• 1993

The German cockroach(Blattelia germanica) population~ were successIVely selected with ch\orpyrifos and permethrin during the six generations. The resulting resistant $R_{chtorpenfos}$(Rc) and $R_{permethnn}$(Rp) stra.ins were studied to investigate the esterase activity by spectrophotometer, filter parper test, and electrophoresis. Esterase-$\alpha$ activities by filter paper test showed 2.65 and LBZ times higher in the Rc and Rp strains than the susceptible strain, respectively. ln the spectrophoLometer method, the esterase activit18s to $\alpha$-and $\beta$-naphthyl acetate were increased 2.34 and 5.28 times in the Rc than susceptible strain, and 1.48 and 2.92 times in the Rp Limn susceptible stram, respectlvely. Zymogram patterns of eslerase isozyme by agarose gel electrophoresis showed totally five bands. The Rc and Rp strains showed two additive bands as, Est-2 and Est-3, which were not shown in the susceptible strain. but the Rp strain dId not show Est-5 bands which was COlumon in the Rc and susceptible strams.

• Korean journal of applied entomology
• /
• v.33 no.3
• /
• pp.166-172
• /
• 1994

This study was conducted to investigate the resistance development and cross-res~stance of house fly (Musco dornestica L.) selected with chlorpyrifos, dichlorvos and permethrin for 11 generations to various p u p s of insectiodes. The resistance ratio (RR) of the chlorpyrifos-selected (Q), the d~chlorvos- selected (&) a d the permethrin-selected (R,) stmlns were 42 0. 38 and 187 tlrnes in female. and 42.0, 4 1 and 16.4 time; in male from the susceptible strain, respectively. The Rc strain showed highest cross-resistance to permethlin among the insectic~des tested: RR=7.5 and 9.6 tunes in female and male, respectively, whereas negatively correlated cross-resistance to propoxur was observed. High cross-res~atance to chlorpyrifos were produced for female (RR= 13.3) and male (RR=15.9) of Rd strain, and female (RR=8.7) and male (RR= 9 7) of R, strain. respectively

• Korean journal of applied entomology
• /
• v.48 no.4
• /
• pp.493-501
• /
• 2009

To investigate resistance of Myzus persicae, which is resistant to etofenprox belonging to pyrethriods, to other insecticides, fenpropathrin and thiamethoxam, mortality and $LC_{50}$ values for these two insecticides against the etofenprox-resistant and -susceptible populations were obtained and EPG analysis for feeding behaviors of thiamethoxam or fenpropathrin treated and non-treated M. persicae was conducted. For fenpropathrin, mortality after 48 h treatment was 28 and 29%, respectively for the resistant and susceptible population. The $LC_{50}$ value was 193.15 and 93.46 ppm, respectively. For thiamethoxam, mortality after 48 h treatment was 87 and 57%, respectively for the resistant and susceptible population. The $LC_{50}$ value was 3.17 and 30.34 ppm, respectively. There was no significant difference in feeding behavior between fenprorpathrin treated and non-treated M. persicae. They showed a continuous feeding pattern. However, contact signal frequency increased in the thiamethoxam treated M. persicae 1 hour after treatment and significant difference was shown.

• Korean journal of applied entomology
• /
• v.28 no.3
• /
• pp.146-153
• /
• 1989

These studies were conducted to investigate development of chemical resistance in two-spotted spider mite (Tetranychus urticae Koch) trough single selection for a given period of time with five acaricides including carbophenothion and ethion of organo-phosphate compound, dicofol of organo-chlorine compound, cyhexatin of organo-tin compound, biphenthrin of synthetic pyrethroid compound; and to examine cross-resistance among the acaricides to the selected populations. The development of chemical resistance of two-spotted spider mite was greatly varied under single selection of the each five acaricides. The carbophenothion- and the ethion-populations at the 22nd and the 24th selected generations showed 156- and 64.1-fold resistant levels, respectively, as compared with the susceptible population. However, the dicofol population at the 28th selected generation exhibited 39.7-fold resistant level, and the biphenthrin population at the 24th selected generation revealed 25.2-fold resistant level, while the cyhexatin population at the 20th selected generation showed 13-fold resistant level. The carbophenothion-selected population showed high cross-resistance to ethion, and low cross-resistance to dicofol and biphenthrin. The ethion-selected population revealed also high cross-resistance to carbophenothion. The dicofol-or the biphenthrin-selected populations, however, exhibited moderate cross-resistance to carbophenothion and ethion. Meantime, the cyhexatin-selected population showed high cross-resistance to ethion and carbophenothion.

• Korean journal of applied entomology
• /
• v.30 no.2
• /
• pp.117-123
• /
• 1991

Acetylcholinesterase(AChE) and esterase activities as mechanisms of resistance to fenobucarb, carbofuran and diazinon in the insecticide-selected brown planthopper strains were investigated. Although there was no significant difference in AChE activity from suscept tible and resistant strains, AChE insensitivity was highly increased in the carbam없e insecticide-selected strains. On the other hand, esterase activity was moderately increa잃d in all the s selected strains. It is concluded that the cross-resistance and the level of resistance in the b brown planthopper can be explained by the combination of altered AChE and high esterase a activity, although a possible involvement of other factor(s) can not be excluded.

• Korean journal of applied entomology
• /
• v.40 no.4
• /
• pp.327-335
• /
• 2001

In order to establish a whitefly control system using conventional groups of insecticide(carbamate, organophosphorus and pyrethroid insecticides), three alternative application methods were designed on the medium growth stage of cucumber. To discriminate the effectiveness of these sequences observed were the residual activity of insecticides, frequency of insecticide applications, residue of insecticides in cucumber leaves, development of insecticide resistance in whitefly, and yield of fruits. Spraying furathiocarb, a carbamate insecticide, was very effective in reducing the frequency of application for the control of white flies. The effectiveness of furathiocarb was enhanced by the potentiation process to carbofuran, the long residual activity, and the lower development rate of insecticide resistance. Methion, an organophosphorus insecticide, did not show resistance development after successive use but resulted in short residual activity. However, other organophosphates, profenofos and phenthoate, lost their activity by the resistance development. Decreasing activity was common to pyrethroids, deltamethrin and zetacypermethrin due to resistance. From these results it could be drawn a conclusion that furathiocarb, a carbamate insecticide, was the most desirable among conventional insecticide groups for the management of greenhouse whitefly population on the cucumber. To prevent an outbreak of the insect pest by various cause, it was recommended to choose acetamiprid, a nicotinoid, which showed very good control efficacy to the resistance insects to conventional insecticides.

• Korean journal of applied entomology
• /
• v.48 no.3
• /
• pp.301-310
• /
• 2009

For the comparing of mortality of the resistance and susceptible population of Myzus persicae, etofenprox was treated in the recommended concentration of 200ppm. Mortalities of resistance population were 16.7 and 36.7%, and susceptible population were 86.7 and 86.7% after 24 and 48 hours treatment, respectively. For the detect of cross resistance to other pyrethroids, 6 pyrethroids were examined. Mortalities of susceptible and resistance populations were 90 and 31% to deltamethrin, 92 and 23% to lambda cyhalothrin, 81 and 14% to cypermethrin, 70 and 20% to $\alpha$-cypermethrin, 29 and 28% to fenpropathrin, 84 and 29% to fenvalerate, respectively. It was showed that resistance populations were generally resistive to other pyrethroids. On the other hands, for recognized ecological characteristic of M. persicae susceptible and resistance populations life table was tested on the pepper leaves in the petri dish and on the plant in the pot. This results were showed that intrinsic rate of increase ($r_m$), net reproduction number ($R_0$) and generation time in day ($T_c$) were significantly different between two population in both tested. However, life span and reproduction period were slightly different between them. Otherwise, feeding behaviors were tested using EPG technique with non- and treated etofenprox. First potential drop time of susceptible and resistance population was 73.5 and 257.9 sec with non-treated and 93.3 and 1076.2 sec after treated, respectively. Electrical probing signals were 8.2 and 48.8 times with susceptible and resistance individuals after treated etofenprox, respectively. It was supposed that the resistance is more probings than susceptible population. After treated, total feeding time have more 6,728.9 sec on resistance than 965.5 sec on susceptible population. So, total non penetration time of susceptible population was 3,000 sec longer than resistance population.

• 농업기술회보
• /
• v.51 no.2
• /
• pp.28-29
• /
• 2014

친환경 안전 농산물에 대한 관심이 높아지면서 환경 친화적인 병해충 방제법에 대한 관심이 커지고 있다. 해충의 친환경 방제 수단인 미생물 살충제의 이용에 대한 관심이 높아지면서 국내에서도 이에 대한 연구가 활발히 진행되고 있다. 그 중에서도 화학농약에 대한 약제 저항성 발현으로 방제가 어려운 가루이류의 살충성 곰팡이를 이용한 방제법 개발은 친환경 농산물 생산에 크게 기여할 것으로 기대된다.