• Journal of Food Hygiene and Safety
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• v.33 no.4
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• pp.280-288
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• 2018

In this study, an approach for the analysis of the five cephalopod species (octopus, long-arm octopus, squid, wet-foot octopus, beka squid) consumed in the Republic of Korea is developed. The samples were collected from the Southeast Asian countries Thailand, Indonesia, Vietnam, and China. The SYBR-green-based real-time qPCR method, based on the mitochondrial DNA genome of the five cephalopods was developed and validated. The intergroup variations in the mitochondrial DNA are evident in the bioinformatic analysis of the mitochondrial genomic DNA sequences of the five groups. Some of the highly-conserved and slightly-variated regions are identified in the mitochondrial cytochrome-c-oxidase subunit I (COI) gene, 16s ribosomal RNA (16s rRNA) gene, and 12s ribosomal RNA (12s rRNA) gene of these groups. To specify each five cephalopod groups, specific primer sets were designed from the COI, 16s rRNA and 12s rRNA regions. The specific primer sets amplified the DNA using the SYBR-green-based real-time PCR system and 11 commercially secured animal tissues: Octopus vulgaris, Octopus minor, Todarodes pacificus, Dosidicus gigas, Sepia esculenta, Amphioctopus fangsiao, Amphioctopus aegina, Amphioctopus marginatus, Loliolus beka, Loligo edulis, and Loligo chinensis. The results confirmed by a conveient way to calculate relative amplification levels between different samples in that it directly uses the threshold cycles (Ct)-value range generated by the qPCR system from these samples. This genomic DNA-based molecular technique provides a quick, accurate, and reliable method for the taxonomic classification of the animal tissues using the real-time qPCR.

• Korean Journal of Food Science and Technology
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• v.46 no.6
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• pp.716-722
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• 2014

Aminopeptidase-active fractions from crude extract of the hepatopancreas of a common squid (Todarodes pacificus) were obtained using acetone (AC; 30-40%) and ammonium sulfate precipitation (AS; 60-70% saturation), anion exchange (AE-II; 0.2 M NaCl) and gel filtration chromatography (GF-I; 30-50 kDa), respectively. The debittering capacity of GF-I fraction based on the aminopeptidase activity (89.2 U/mg), recovery (56.6%) and sensory evaluation (1.0) was better than that of other fractions. Release of amino acids increased as incubation time was increased, and the bitterness of the enzyme reaction mixtures decreased. Incubation with the GF-I fraction for 24 h resulted in the hydrolysis of several peptides, as revealed by reverse-phase HPLC profiles. Peaks 3, 5 and 6 showed the decreased area (%), whereas peaks 1, 2 and 4 showed the increased area. The GF-I fractions were found to be suitable for reducing bitterness in protein hydrolysates by catalyzing the hydrolysis of bitter peptides.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.35 no.2
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• pp.102-117
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• 1999

Vertical distribution of squids in relation to oceanographic structure was analyzed on the basis of experimental squid hand jigging fishing by R/V Pusan 851 in the area of 34°∼47° N, 150° E∼170°W in the North Pacific in summer from 1987 through 1993 with exception of 1991. The 6 species of squids showed different patterns of vertical and horizontal distribution as following; Boreopacific gonate squid (Gonatopsis borealis) were mainly caught in the layer of 71-80m fishing depth of the Subarctic Domain with water temperature of 6∼11℃ and salinity of 32.2∼33.6‰ and distributed in the latitudes of 41°∼43° N. Boreal clubhook squid (Onychoteuthis borealijaponica) were mainly caught in the layer of 11∼20m fishing depth of the Subarctic Domain with water temperature of 10∼12℃ and salinity of 32.9∼33.6‰ and distributed in the latitudes of 41°∼42N°. Tapanese flying squid (Todarodes pacificus) were mainly caught in the layer of 11∼20m fishing depth of the Transition Zone and the Subtropical Domain with water temperature of 15∼18℃ and salinity of 33.6∼34.0‰ and distributed in the latitudes of 40°∼42°N. Neon flying squid (Ommastrephes bartrami) were mainly caught in the layer of surface∼10m fishing depth of the Subarctic Convergence Zone and the Transition Zone with water temperature of 16∼17℃ and salinity of 33.7∼34.4‰ and distributed in the latitudes of 39 °∼41°N. Luminous flying squid (Symplectoteuthis luminosa) were mainly caught in the layer of 11 20m fishing depth of the Transition Zone and the Subtropical Domain with water temperature of 18∼20℃ and salinity of 33.8∼34.6‰ and distributed in the latitudes of 37°∼39°N. Purpleback flying squid (Symplectoteuthis oualaniensis) were mainly caught in the layer of surface∼10m fishing depth of the Subtropical Domain with water temperature of 24∼25℃ and salinity of 34.2∼34.4‰ and distributed in the latitude of 36°∼37°N.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.37 no.2
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• pp.85-94
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• 2001

The vertical distribution and activity patterns of fishes during the evening and morning transitions were investigated acoustically and by pelagic trawling on October 2-10, 1997 in the Korean Eastern Sea. The acoustic data were collected from four stations with a scientific echo sounder operating at a frequency of 38 kHz, and the echogram was used to analyze the diel vertical migration of fish. Biological sampling was accomplished by pelagic trawling to identify fish species recorded on the echograms, and the species and length compositions were investigated. Vertical profiles of water temperature, salinity at the trawl station were taken with a CTD system and were related to the diel movement and the depth distribution of fish. During the day, one group of fish mainly distributed above the depth layer of 50 m, the other group was at deeper water, approximately 250 m, but began to migrate toward the surface before sunset with a time difference. During the night, they were dispersed between the surface and the depth of 125 m. Just prior to sunrise, the scattering layer was divided with 2 groups and began to migrate downwards, respectively. Several trials of pelagic trawling for scattering layers suggest that the most dominant scatterer in the survey area of this study was Japanese flying squid, Todarodes pacificus..

• Journal of Environmental Science International
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• v.26 no.2
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• pp.173-181
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• 2017

Catch data for the common squid (Todarodes pacificus), classified by squid-jigging fisheries per grid (size: $0.5^{\circ}latitude{\times}0.5^{\circ}longitude$), and the water temperature values KODC (Korea Ocean Data Center) were collected for the 1980-2009 period to study the changes in squid distribution and migration with climate regimes (1980s, 1990s, and 2000s). The primary fishing period in the 1990s and 2000s was approximately 2-3 months earlier than that in the 1980s. Especially in the East Sea, the fishing grounds in the 1980s stayed longer at high latitudes than those in the other decades. Moreover, in the 1980s, centers of the fishing ground were located near the Yamato bank (central East Sea), whereas in the 1990s and 2000s, they were situated near the southeastern coast of the Korean peninsula.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.6
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• pp.472-479
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• 2006

The sexual maturity and reproductive cycle of the common squid, Todarodes pacificus captured from the East Sea, Korea, between January 2004 and January 2006, were investigated by documenting changes in the gonadosomatic index (GSI), gonad development, and oocyte size frequency distribution. The GSI of females began to increase in July, reached a maximum in August, and then gradually decreased. The GSI of males increased from July to March. Using gonad histological observations, we identified four oocyte developmental stages. The changes in GSI and gonad tissue resulted in the classification of the annual reproductive cycle into the following four successive stages: immature (April to June), growing (June to July), mature (July to August), and ripe and spawning (August to March). According to the oocyte diameter size frequency distribution in the ovary, this species appeared to have asynchronous oocyte development and one spawning time.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.3
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• pp.338-343
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• 2018

Recently, commercial catches of the common squid Todarodes pacificus have dramatically decreased in Korean and Japanese waters. The relationship between common squid catches and environmental factors was investigated using squid catches, climate indices and observed seawater temperatures in Korean waters. Common squid consist of three spawning stocks: autumn, winter, and summer. The autumn stock is the largest in Korea, and its main fishing season appears to have shifted from September in the 1980s to October in the 1990s. We observed negative correlations between the spring Southern Oscillation Index and Korean catches and between the winter Pacific Decadal Oscillation and Japanese catches. Despite global warming, no conspicuous increases in October seawater temperatures have been observed at 10 and 50 m in Korean waters since the mid-1900s. Instead, the 50 m water layer of the East Sea appears to be gradually cooling. Moreover, temperatures at 50 m in the East Sea and the South Sea were significantly negatively correlated with squid catches in Korea and Japan, respectively. Our preliminary analysis indicates a link between climate change, seawater temperature, and squid catches in Korean waters, which helps to inform the direction of subsequent research to identify the cause of rapid decreases in this squid resource.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.6
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• pp.709-718
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• 2019

Cephalopods, such as the Japanese flying squid Todarodes pacificus and golden cuttlefish Sepia esculenta, are an important food source for all toothed whales, particularly bottlenose dolphins in costal and offshore waters around the Korean Peninsula. A controlled laboratory experiment was conducted to investigate the broadband acoustic backscattering from live individuals of these two cephalopod species using linear chirp signals (100-200 kHz). The backscattered echo signal was measured at about 1° intervals from -45° (head down) to +45° (head up) in the dorsal plane. The species-specific, frequency-dependent scattering characteristics were investigated by comparing the relationship between the wavelength-normalized backscattering cross-section (σ/λ²) and the wavelength-normalized fish length L/λ for each species. The estimated σ/λ² value for Japanese flying squid was 9.51 at an L/λ range of 12.79-30.27 (mean, 21.26). This was approximately 7% of the σ/λ² value (136.1) for golden cuttlefish at an L/λ range of 9.07-25.49 (mean, 15.77).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.1
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• pp.42-48
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• 2006

Analysis of stomach content for common squid was carried out to investigate the difference in seasonal and ontogenetic feeding behavior. Totals of 1368 common squid were collected monthly off Busan from September 2004 to August, 2005. Size range of common squid was 8.5-31.8 cm mantle length (ML) during the study period. Of the 1368 stomachs examined, 599 specimens (43.8%) were empty. Stomach contents indicated that common squid was carnivore: Large portion of stomach contents consisted of fish and mollusca (mainly common squid). The stomach contents index (SCI) seemed to increase with size, though there was no statistical difference between size classes significantly. Cannibalism was minimum in size range of 15-20 cm ML, but was steadily increased with size. Cephalopods were the major prey during spring and summer when common squid were small, but fish replaced it during autumn and winter when they became large.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.5
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• pp.769-781
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• 2021

Given data about the annual fishery yield of the common squid Todarodes pacificus, and the catch-per-unit-effort (CPUE) data from multiple fisheries from 2000-2018, we applied a Bayesian state - space assessment model for the squid population. One of our objectives was to do a stock assessment, simultaneously incorporating CPUE data from the following three fisheries, (i) large trawl, (ii) jigger, and (iii) large purse seine, which comprised on average a year about 65% of all fisheries, allowing possible correlations to be reflected. Other objectives were to consider both observation and process errors and to apply objective priors of parameters. The estimated annual exploitable biomass was in the range of 3.50×10⁵ to 1.22×10⁶ MT, the estimated intrinsic growth rate was 1.02, and the estimated carrying capacity was 1,151,259 MT. Comparison with available results from stock assessment of independently analyzed single fisheries revealed a large difference from the estimated values, suggesting that stock assessment based on multiple fisheries should be performed.