• Journal of Plant Biology
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• v.36 no.4
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• pp.415-423
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• 1993

대두의 uricase II cDNA를 탐침으로 plaque 혼성화 방법에 의해 해녀콩의 뿌리를 cDNA library로부터의 두 개의 phage 클론(λCINUO-01, λCINUO-02)을 선별하였다. 두 phage 클론은 약 1.6 kb와 1.0 kb의 insert를 갖고 있었으며 이들의 염기서열을 결정하기 위하여 pUC19과 pBSKS vector에 subcloing(pcCLNUO-01, pcCLNUO-02)하였다. Sanger법에 의해 염기서열을 결정한 결과, 두 클론은 각각 1,611 bp와 1,024 bp로 이루어져 있었으며 pcCINUO-01은 308개의 아미노산, pcCINUO-02는 301개의 아미노산을 암호화하는 open reading frame(ORF)을 갖고 있었다. 두 클론의 ORF의 염기서열은 대두의 uricase II와 각각 88.9%, 89.3%의 상동성을 보여주었으며, 아미노산 서열은 84.1%, 85.4%의 상동성을 보여주었다. pcCINUO-01의 경우, 종결코돈으로부터 313 NT 하류쪽에 진핵생물의 poly(A) 첨가신호인 AATAAA 서열이 존재하였으며 이로부터 21 NT 하류쪽에 17 잔기의 poly(A)가 존재하였다. 두 클론의 염기서열에서 추정된 아미노산 서열의 카르복시 말단에는 세포질에서 합성된 몇몇 단백질들이 peroxisome으로 수송되는데 필요한 신호서열인 Ser-Lys-Leu-COOH 서열이 존재하고 있었다. 두 클론의 염기서열을 토대로 아미노산 조성을 살펴본 결과, 염기성 아미노산(Arg, His, Lys)과 산성 아미노산(Asp, Glu)이 각각 46 대 35, 47 대 35의 비를 보여주었는데 이는 uricase II 단백질의 염기성 성질을 보여주는 결과로 추정된다. Northern 혼성화 결과 해녀콩에서 uricase II는 뿌리혹에서만 특이적으로 발현됨을 알 수 있었고 게놈 혼성화 반응 결과는 uricase II 유전자가 해녀콩 게놈상에 유전자 가족으로는 존재할 수 있음을 보여주었다.

• Analytical Science and Technology
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• v.5 no.1
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• pp.33-39
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• 1992

THF-based crown-4 of 16-membered rings having tetrahydrofuran unit was synthesized by an acid-catalyzed condensation of furan and acetone followed by hydrogenation in an effort to obtain highly elective ionophores for lithium ions. The new ionophore was compared with previously reported ionophores under similar measurement conditions with the same plasticizer, tris(2-ethylhexyl) phosphate in poly(vinyl chloride)(PVC) membrane electrodes. Separate solution method was used to determine relative selectivity coefficients for the electrode. The selectivity coefficients($K_{LiM}^{POT}$) of lithium over ammonium, alkali and alkaline earth metal ions go from about $2.4{\times}10^{-1}$ to $2.3{\times}10^{-4}$ to working range and pH dependence have also been studied.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.05a
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• pp.805-805
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• 1995

핵연료저장시설의 화재 등 극단적인 사고조건하에서 $UO_2$ 소결펠렛의 습식산화와 건식산화에 대한 연구를 수행하였다. 손상된 지르칼로이 피복관 속의 $UO_2$ 소결펠렛을 산성분위기의 습윤조건하에서 산화시킬 때의 $UO_2$ 펠렛의 산화속도는 IDR(mg/$\textrm{cm}^2$.min) = 1.55 [H$^{+}$]$^{1.21}$ 로 나타났다. 또한 습윤조건하에서 $UO_2$ 분말에 알카리 및 알카리 토금속 산화물, 그리고 백금족 및 회토류 산화물 등과 같은 불순물들이 존재할 때의 산화속도를 조사하였으며 이들에 대한 영향도 관찰하였다. 핵연료저장시설의 가상화재를 바탕으로 한 400~$700^{\circ}C$의 온도범위에서, 피복관이 씌워진 $UO_2$ 소결펠렛의 건식산화반응을 조사한 바 $UO_2$ 소결펠렛은 산화초기에 U$_4$O$_{9}$ 또는 U$_3$O$_{7}$ 등의 중간상 형성에 따른 3-4%의 부피축소에 의해 결정립계 균열이 일어나고, $600^{\circ}C$ 이하에서는 온도증가에 따라 중간상에서 U$_3$O$_{8}$ 상으로의 상변화에 의한 부피팽창으로 피복관의 변형과 함께 산화속도의 가속을 발견할 수 있었고, $600^{\circ}C$ 이상에서는 핵연료소자의 소성변형으로 인한 산화속도의 지연을 발견할 수 있었다. 또한 $UO_2$ 펠렛의 건식산화거동은 기체-고체 반응시의 전형적인 형태인 shrinking core model에 잘 적용될 것으로 판단되었다.

• Korean Journal of Soil Science and Fertilizer
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• v.27 no.3
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• pp.149-157
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• 1994

The conditions for formation of allophane in volcanic ash soils in Cheju Island were investigated. Soils of toposequence distributed along the sourthern slope of Mt. Halla, and the major soil groups such as dark brown soils, very dark brown soils, black soils, and brown forest soils were colleted and analyzed for Al, Fe and Si extracted with solutions of pyrophosphate and oxalate. Mean annual temperature decreased $0.8^{\circ}C$ and mean annual precipitation increased 110mm with increase elevation of 100m. Organic carbon content increased and soil pH decreased with elevation, and the formation of allophane in soils formed a climosequence. Dark brown soils widely distributed in the northern and western coastal areas, where the mean annual precipitation ranged 1,240~1,420mm and the evaporation ranged 1,290~1,320mm, contained only small amounts of allophane and Al-humus complexes. For other soils, organic carbon content, pyrophosphate extractable Al, and $Al_p/Al_o$ were inversely correlated with $pH(CaCl_2)$. Allophane content showed close relationships wlth $pH(CaCl_2)$, and inverse relationships with organic carbon content and $Al_p/Al_o$.

• Journal of the Mineralogical Society of Korea
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• v.15 no.2
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• pp.114-121
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• 2002

Buffer capacities for two Bo horizon soils or Andisols developed from different parent materials have been investigated. The titration curves from column leaching experiment show that buffering occurred at pH 4.0 and 6.0. The buffer intensity or soil developed from pyroclastic materials (P-soil) is higher than that from basalts (B-soil). From batch test we have found that proto-imogolite and/or imogolite may control Al solubility as well as $Al(OH) _3$in the moderate acid condition. The buffer intensities ($\beta$) of P-soils were plotted on the theoretical buffering curve of $Al(OH)_3$, while $\beta$ of B-soils approached to that of proto-imogolite, which shows the solubility of short-range-order materials in P-soil control the buffer capacity. Buffering at pH 6.0 is thought to be the result of dissolution of some silicate clays and exchange reactions between $H^{+ }$and base-forming cations. Considering the amount of annual acid precipitation, aluminum solubility of Andisols, and the low BS (Base Saturation percentage), it can be predicted that prolonged acid precipitation will reduce the buffer capacity of soils and lead to soil acidification.

• Korean Journal of Soil Science and Fertilizer
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• v.4 no.1
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• pp.101-107
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• 1971

1. 수도(水稻)의 생육기간(生育其間)만이라도 심수하(甚水下)에 있어야하는 논은 투수성(透水性)의 영향(影響)을 받아야 하고, 동영향(同影響)은 수도생육(水稻生育)과 관련(關聯)을 갖는다. 즉(卽) 투수성(透水性)이 클 경우(境遇)에는 물의 소비(消費)가 많을 뿐만이 아니라 영양분(營養分)의 유실(流失) 및 유효화(有效化)가 억제(抑制)되고, 투수성(透水性)이 적을 경우(境遇)에에는 심(甚)한 환원상태(還元狀態)의 발달(發達)로 유해성분(有害成分)이 집적(集積)되어 수도(水稻)의 생육(生育)은 해(害)를 입게된다. 이렇게 생각하면 투수성(透水性)으로 본 벼 생육(生肉)에 가장 적합(適合)한 토양조건(土壤條件)이 있을 것인바 이 조건(條件)은 토양(土壤)의 종류외(種類外)에도 수도(水稻)의 품종(品種)이나 생육시기(生育時期)에 따라 다른것이다. 2. 투수성(透水性)이 큰 토양(土壤)에는 잘 유거(流去)되지 않는 비료(肥料)(유기물비료(有機物肥料)나 용성연비(熔成憐肥) 등(等))를 쓸것이며, 투수성(透水性)이 적고 유해성분(有害成分)이 집적(集積)되기 쉬운 토양(土壤)에는 류산근(류硫酸根)이나 유기질(有機質) 비료등(肥料等) 유해성분(有害成分)의 집적(集積)을 증가(增加)할 우려가 있는 비료(肥料)의 시용(施用)을 삼가야 한다. 3. 기왕(旣往) 국내(國內)에서 실시(實施)한 시험결과(試驗結果)에 의(依)하면 중점토(重粘土)에서는 연산(憐酸), 중점토(重粘土)와 퇴화염토(退化鹽土)에서는 가리결핍(加里缺乏)을 일으키기 쉬운것 같으나 그 밖의 토양(土壤)에서는 뚜렷한 경향(傾向)이 인정(認定)되지 않는다. 4. 투수성(透水性)이 큰 토양(土壤)에서는 산적토(山赤土)를 객토(客土)하는 경우(境遇)가 많은 바 이때에는 금비(金肥) 특(特)히 질소질비료(窒素質肥料)를, 객토(客土)하지 않았을때 보다 많이 써야 하는것이 일반적(一般的)이다. 5. 투수성(透水性)이 약(弱)하고 유해성분(有害成分)이 집적(集積)되기 쉬운 논에는 우선(于先) 석회(石灰)(소석회(消石灰))를 시용(施用)하여 토양반응(土壤反應)을 높여야 하며 심(甚)한 노후답(老朽畓)이나 퇴화염토(退化鹽土)에는 석회(石灰)와 아울러 규산질(珪酸質) 비료(肥料)를 시용(施用)한 연후(然後)에 비료(肥料) 삼요소(三要素) 시용(施用)을 고려(考慮)해야 할 것이다. 산성(酸性)으로 기울어져있는 많은 우리나라 논 토양(土壤)에는 알칼리성(性) 비료(肥料)인 용성연비(熔成憐肥)를 시용(施用)하는 것이 효과적(效果的) 일것이다. 6. 소석회(消石灰)와 규산(珪酸)의 시용(施用)은 연산(憐酸)과 가리(加里)의 요구량(要求量)을 증가(增加) 할것이며 비료(肥料) 삼요소(三要素)에 대(對)한 수도(水稻)의 응수곡선(應酬曲線)의 모습을 기왕(旣往)의 것과 달리 할것이다.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.3
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• pp.173-179
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• 1989

Characterization and classification of the potential acid sulfate soils found on flood-plains in Yeongnam area were summarized as follows: 1. The "Potential acid sulfate soil" layer(s) were appeared in the around 2-4m substrata of soil profiles and characterized by the fine texture, high reduction and physical unripened soft mud deposits or having higher contents of organic matter with dark color. 2. The contents of total sulfur (T-S) in those soils were ranged around 0.45-0.9% and the materials exhibited a strong acidity upon the oxidation with $H_2O_2$. Although the T-S contents was low as much as 0.15%, the sulfidic materials were also acidified strongly by the oxidation with $H_2O_2$ in the condition of lower content of carbonates. As defined in Soil Taxonomy of USDA, most of the sulfidic materials contained less than 3 times carbonate ($CaCO_3$ equivalent wt. %), but there were some which abundant in shell fragments, contained more than 3 times carbonate by weight percentage and that not much acidified by the oxidation with $H_2O_2$. 3. The contents of T-S correlated negatively with the pH oxidized by $H_2O_2$ and with the fizzing time (minutes) due to addition of $H_2O_2$. 4. The potential acid sulfate soils could be defined as soil materials that had sulfidic layer(s) more than 20cm thick within 4m of the soil profile and contained more than 0.15% of T-S with less than 3 times carbonate ($CaCO_3$ equiv. %). A tentative interpretative soil classification system was proposed, i.e., "Weak potential acid sulfate (T-S, 0.15-0.5%)", "Moderate potential acid sulfate (T-S, 0.5-0.75%)", and "Strong potential acid sulfate (T-S, more than 0.75%)". Finally, it was proposed that the "Detailed soil survey with high intensity" should be carried out in the areas of agricultural engineering works such as arableland readjustment works, in advance.

• Journal of the Mineralogical Society of Korea
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• v.22 no.1
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• pp.35-47
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• 2009

The deteriorations of stone monuments located in Gyeongju area are predicted in a modeling study. Artificial rain and accelerated weathering test are here applied to the Gyeongju Namsan granite and cement mortar. They are reacted with pH 4.0, pH 8.0 and pH 5.6 rain, respectively. The two former values are the limited acidity values in the Gyeongju (the acidity of rain of the Gyeongju were pH $4.93{\sim}6.39$ in 2005) and the latter is the limited acidity of acid rain. The rains of pH 5.6 and pH 8.0 reach close to a value of pH 7.0 after the reaction with the Gyeongju Namsan granite. After application of the artificial rain and weathering test, the weight of specimens were reduced and the contents of soluble ions in the specimens were increased. These results are attributable to solution of minerals in the specimens. At first, the microlithic have dissolved in ground from the reaction with acid rain. And then mainly quartz, plagioclase and orthoclase bound by the microlithic are disintegrated. The cement mortars are dissolved after the reaction with not only acid but alkali rain. The concentrations of ions in the dissolved cement mortar are higher than those in granite.

• Korean Journal of Soil Science and Fertilizer
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• v.4 no.2
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• pp.187-192
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• 1971

This paper deals mainly with the genesis and classification of the Jeonnam series. These soils have brown to dark brown silt loam and silty clay loam A horizon(strong brown or reddish brown where eroded). Argillic B horizons are dominantly red or yellowish red silty clay loam to silty clay with moderately developed subangular blocky structure and with thin clay cutans on the ped faces. The C horizons are strongly and very deeply weathered strong brown, yellowish brown, pale brown and reddish yellow silty clay loam and sandy loam granitic saprolite. Content of clay increases with depth to a maximum between 100cm. Percolating water seems to be responsible for transportation and oriented deposition of clay. Chemically, soil reaction is strongly acid to medium acid throughout the profile. The content of organic matter is 1 to 2 percent, and decreases regularly with depth. Base saturation is low, based on amount of extractable cations. Characterisltically the Jeonnam series are similar to Red-Yellow Podzolic soils of the United States and are similar to Red-Yellow soils of the Japan. In the writer's opinion the Jeonnam soils are classified as Red Yellow soils. According to USDA 7th approximation, this soil can be classified as Typic Hapludults and in the FAO/UNESCO World Soil Map as Helvic Acrisols.

• Journal of the Korean Data and Information Science Society
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• v.28 no.6
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• pp.1457-1469
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• 2017

Private education expenses is one of the key issues in Korea and there have been many discussions about it. Academically, most of previous researches for private education expenses have used multiple regression linear model based on ordinary least squares (OLS) method. However, if the data do not satisfy the basic assumptions of the OLS method such as the normality and homoscedasticity, there is a problem with the reliability of estimations of parameters. In this case, quantile regression model is preferred to OLS model since it does not depend on the assumptions of nonnormality and heteroscedasticity for the data. In the present study, the data from a survey on private education expenses, conducted by Statistics Korea in 2015 has been analyzed for investigation of the impacting factors for private education expenses. Since the data do not satisfy the OLS assumptions, quantile regression model has been employed in Bayesian approach by using gibbs sampling method. The analysis results show that the gender of the student, parent's age, and the time and cost of participating after school are not significant. Household income is positively significant in proportion to the same size for all levels (quantiles) of private education expenses. Spending on private education in Seoul is higher than other regions and the regional difference grows as private education expenditure increases. Total time for private education and student's achievement have positive effect on the lower quantiles than the higher quantiles. Education level of father is positively significant for midium-high quantiles only, but education level of mother is for all but low quantiles. Participating after school is positively significant for the lower quantiles but EBS textbook cost is positively significant for the higher quantiles.