• Title/Summary/Keyword: 산란키트

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Establishment of the Successive Rearing Method of Cabbage Butterfly, Pieris rapae L. in a Room Condition (배추흰나비의 실내 계대사육법 확립)

  • 설광열;김남정
    • Korean journal of applied entomology
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    • v.40 no.2
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    • pp.131-136
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    • 2001
  • Cabbage butterfly, Pieris rapae was reared in a room to establish a year-round rearing system. The eggs oviposited by the parent fed on host plant showed 89.2% of hatchability and hatched in 3.9 days after oviposition. The larval period was 18.1 days under high temperature, long day condition ($25^{\circ}C$, 16L : 8D), showing 97.8% pupal ratio and emergence rate. However, under low temperature, short day condition ($21^{\circ}C$, 10L : 14D) the larval period extended to 23.6 days and the pupal ratio was 70%. All of those pupae went into diapause. The oviposition preference experiment on different hosts (Chinese cabbage, cabbage, tulip and kale) showed that hot-water extract was preferred over methanol extracts or squeezed raw juices. The host preference showed that Chinese cabbage was less preferred than the other three. The artificial ovipositing kit was constructed for the oviposition in a room. The 48-hours old eggs could be stored for 7 days at$ 5^{\circ}C$ and showed 70% of hatchability. Non-diapausing pupae could be stored for 30 days at 5 to $15^{\circ}C$, showing 85% of emergence rate. However, the pupae stored at $5^{\circ}C$ showed longer storage period and higher emergence rate. The systematic successive rearing method of cabbage butterfly in a room was completed, based on the above experiments.

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Establishment of the Successive Rearing System of Brush-footed Butterflies (Lepidoptera: Nymphalidae) (네발나비과 나비류의 계대사육법 체계확립)

  • Seol, Kwang-Youl;Kim, Nam-Jung;Hong, Seong-Jin
    • Korean journal of applied entomology
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    • v.44 no.4 s.141
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    • pp.257-264
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    • 2005
  • In order to establish the successive rearing system brush-footed butterflies (Lepidoptera : Nymphalidae) were reared in a room. Artificial diets were developed for a year-round rearing. Bu-diet was best to rear these butterflies among 3 kinds of diet used. The freeze-dried host plant leaf powder in diet was better than heat-dried one $(60^{\circ}C)$ in the growth of larvae. The rearing results were best in the diet C/N ratio was 1:1. The 24-hrs old eggs could be stored for 5 days at $15^{\circ}C$ or for 3 days at $5^{\circ}C$ and showed 75% of hatchability. On the other hand, pupae could be stored for maximum 15 days at $15^{\circ}C$ because the emergence of abnormal adults appeared much more as the cold storage period got longer. And the adult was able to be stored until 60 days at refrigerator without relation of nectar-sucking period before cold-storage and storage temperature. Also a simple artificial ovipositing kit was devised by ${\Phi}9$ cm of petri-dish and a female oviposited $278{\pm}27$ of eggs with adding the ether extract of host plant to this kit. The systematic successive rearing method of brush-footed butterflies in a room was completed.

Development of Infectious Bronchitis Virus (IBV) ELISA Kit for Detection of Antibodies against Nephropathogenic IBV Vaccine (국내회사와 다국적기업 제조 ELISA 키트의 전염성 기관지염 백신에 따른 항체 검출능 비교)

  • Kim, Kyu-Jik;Kim, Jun-Young;Youn, Ha-Na;Ju, Hyo-Sun;Lee, Da-Yeah;Song, Chang-Seon
    • Korean Journal of Poultry Science
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    • v.45 no.1
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    • pp.17-28
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    • 2018
  • Infectious bronchitis virus (IBV) is an economically important disease in the poultry industry worldwide. This disease commonly manifests respiratory signs, poor egg quality, and decline in egg production. Since IBV is a RNA virus, the emergence of new variant strains is continuously reported and the immunization of susceptible chickens with only one antigenic type of the virus has been shown to induce partial or no protection against other unrelated types. Therefore, it is difficult to diagnose IBV due to variants serotypes. In this study, we collected serum from various ages of Broiler GP (Grandparent) to Layer CC (Commercial chick) and performed detectability comparison test between domestic company and multinational company manufacturing ELISA kit. Results of this experiment suggest that domestic company manufacturing ELISA kit is more sensitive to infectious bronchitis antibody than that of the multinational company. Our findings also suggest that antibody's change trends after infectious bronchitis vaccination. Thus, the use of appropriate kit for domestic situations is important.

Evaluation of Avian Influenza and Newcastle Disease Virus Detection Kit using Field Samples from Domestic and Semi-domestic Birds (닭과 야생사육조류로부터 야외샘플을 사용한 조류인플루엔자와 뉴캣슬병 바이러스 검출 키트의 평가)

  • Rahman, Md. Siddiqur;Malek, Md. Abdul;Islam, Md. Alimul;Uddin, Muhamad Jasim;Ahasan, Md. Shamim;Chakrabartty, Amitavo;Sakib, Md. N.;Chae, Joon-Seok
    • Journal of Veterinary Clinics
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    • v.29 no.4
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    • pp.309-314
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    • 2012
  • The study was undertaken to evaluate sensitivity and specificity of rapid Avian Influenza (AI) and Newcastle Disease virus (NDV) combo antigen kits from field samples of domestic (broiler and layer chicken, native chicken) and semi-domestic (duck, goose, pigeon and quail) birds of Bangladesh. Samples were collected from naturally infected AI suspected domestic and semi-domestic birds of five different outbreak areas in Bangladesh. From each area two birds were selected for sampling, and from each bird three types of samples (tracheal, cloacal and oro-nasal swabs) were collected. A total of 210 field samples from a total of 70 birds were collected and tested using AI and NDV combo antigen rapid diagnostic kits in the study. All three different samples from a bird showed similar pattern of reaction. Out of 210 samples, 15 samples (5 birds), 63 samples (21 birds) and 27 samples (9 birds) were positive for AIV, NDV and both for AIV and NDV, respectively; whereas the remaining birds were negative for either AIV or NDV in this screening test. Among the five AIV positive, a layer chicken from wet market in Mymensingh, Netrokona, Gibandha and Kurigram and a native chicken from wet market in Kurigram area was positive to AIV. The semi-domestic birds are either positive to NDV or free from both AIV and NDV. This study revealed that the AIV and NDV rapid diagnostic kits could be effectively use to diagnose the respective virus in trachea, oro-nasal and cloacal samples simultaneously. AIV-NDV combo Ag test result clearly indicates that the test kit designed for AIV and NDV could diagnose the disease rapidly with less effort and higher scientific know how which could be used for the detection of AIV and NDV using field samples in large scale.

월간닭고기

  • 한국위생계육산업협회
    • Monthly Korean Chicken
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    • v.2 no.7 s.13
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    • pp.2-8
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    • 1996
  • 육류중 유해물질 검사 강화 - 육계 초기 사료제한 연구 - 잔류 항생제 정성검사 키트 개발 - 일본인 육류섭취량 증가추세 - 축산폐수 단속기중 강화 - 축산물 소비촉진 홍보대책 필요 - 사료용 부연료 할당관세 $2.5{\%}$적용 - 가금티푸스 예방약 개발 - 가금인플루엔자 방역대책 시급 - 종계 ND 발생지역 백신 프로그램 - 수입종란 외래질병 매개 가능성 - 안전축산물 생산운동연합회 발족 - 중국, 닭고기 산업 잠재력 무한 - 삼계탕 수출 급속 성장중 - 소비자 단체 임원 닭 생산현장 방문 - 산란노계 처리 계속 어려워 - 인도, 육계수입 규제완화 재검토 - 축산기자재 구입 외화자금 사용가능 - 미국, 도계전 급이중단 실험 - 러시아, 수입쿼터제 부과 - 호주, 브로일러 산업 지속적 성장 - 배합사료업체 사료원료 선물구매 기피

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Preliminary Study on Performance Evaluation of a Stacking-structure Compton Camera by Using Compton Imaging Simulator (Compton Imaging Simulator를 이용한 다층 구조 컴프턴 카메라 성능평가 예비 연구)

  • Lee, Se-Hyung;Park, Sung-Ho;Seo, Hee;Park, Jin-Hyung;Kim, Chan-Hyeong;Lee, Ju-Hahn;Lee, Chun-Sik;Lee, Jae-Sung
    • Progress in Medical Physics
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    • v.20 no.2
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    • pp.51-61
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    • 2009
  • A Compton camera, which is based on the geometrical interpretation of Compton scattering, is a very promising gamma-ray imaging device considering its several advantages over the conventional gamma-ray imaging devices: high imaging sensitivity, 3-D imaging capability from a fixed position, multi-tracing functionality, and almost no limitation in photon energy. In the present study, a Monte Carlo-based, user-friendly Compton imaging simulator was developed in the form of a graphical user interface (GUI) based on Geant4 and $MATLAB^{TM}$. The simulator was tested against the experimental result of the double-scattering Compton camera, which is under development at Hanyang University in Korea. The imaging resolution of the simulated Compton image well agreed with that of the measured image. The imaging sensitivity of the measured data was 2~3 times higher than that of the simulated data, which is due to the fact that the measured data contains the random coincidence events. The performance of a stacking-structure type Compton camera was evaluated by using the simulator. The result shows that the Compton camera shows its highest performance when it uses 4 layers of scatterer detectors.

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