• Title/Summary/Keyword: 사포닌

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Immunochemical Assay for Korean Ginseng Saponins I Synthesis of Ginsenoside-Protein Conjugate (인삼사포닌의 면역화학적 분석법(I) 인삼사포닌-단백질 결합체의 합성)

  • 한병훈;한용남
    • YAKHAK HOEJI
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    • v.25 no.2
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    • pp.43-47
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    • 1981

  • In an attempt to obtain a saponin antigen, ginsenoside Rg$_{1}$ of Korean ginseng was condensed with bovine serum albumin through a series of modification in the side chain structure of ginsenoside Rg$_{1}$ to prepare a reactive intermediate $Rg_{1}$ azide. The modification of ginsenoside $Rg_{1}$[1] yielded $Rg_{1}$ decacetate [II], mp 252, $Rg_{1}$ acetate-glycol [III], mp 263, $Rg_{1}$ acetate-trisnoraldehyde [IV], mp 231, $Rg_{1}$ acetate-carboxylic acid [V], mp 282, $Rg_{1}$ acetate-methyl ester [VI], mp 271, $Rg_{1}$ hydrazide [VII], mp 220, and finally a reactive intermediate $Rg_{1}$ azide [VIII].

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Surface Activities of Ginseng Saponins and Their Interactions with Biomolecules'(V) Ginseng Saponins Can Be Vsed in Cytochrome c Isolation (인삼사포닌의 계면활성 및 생체 분자와의 작용 : (V) 미토콘드리아 Cytochrome c 단리에서 인삼사포닌의 추출효과)

  • Lee, Jae-Yang;Lee, Sang-Jik
    • Journal of Ginseng Research
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    • v.19 no.2
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    • pp.122-126
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    • 1995

  • The effects of treating bovine heart mitochondria with potassium chloride and surfactants such as digitonin and n-dodecy-$\beta$-maltoside (DMS) including plant saponins on extracting cytochrome c were examined. The spectra given by the cytochrome c-containing solutions from the extraction were inspected to ascertain whether ginseng and bellflower saponins could be used instead of the generally- employed surfactants of digitonin and DMS. These studies implied that the effect of ginseng saponins is superior to that of digitonin but inferior to that of DMS, and give rise to the idea of substitutional property of ginseng saponins for the widely-employed surfactants in the extraction of mitochondria intermembrane cytochrome c. The substitution for the solubilizing surfactants by bellflower saponins could, however, not presumably be anticipated; while ginseng saponin mixture are a suitable substitute.

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