• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.107-114
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• 1997

Various fractions of apple fibers such as crude pulp, total dietary fiber, soluble dietary fiber, and insoluble dietary fiber were prepared and added to the proteose peptone-yeast extract-fildes (PYF) media to see their effects on the growth of type cultures of intestinal bacteria. Most microbes tested in this experiment grew well in PYF media with the soluble dietary fiber of apple than with the insoluble dietary fiber. Especially Bifidobacterium species such as B. adolescentis, B. animalis, B. infantis, B. longum, B. thermophilum showed higher growth in PYF media containing the soluble dietary fiber than other fiber fractions. However, pectin-added media didn't promote the growth of most microbes used in the experiment. In the in vitro mixed culture using rat feces as starter, the addition of the soluble dietary fiber or pectin to the basal medium showed larger proportion of Bifidobacterium species in total bacteria than that of glucose.

• Korean journal of applied entomology
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• v.55 no.3
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• pp.235-243
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• 2016

The winter cherry bug, Acanthocoris sordidus (Thunberg), is an insect pest hat damages plants from Solanaceae and Convolvulaceae. The developmental period from egg to adult averages 76 days at $25^{\circ}C$. Adult egg-laying occurred irregularly via spawning, averaging 195 (up to 468) eggs per individual on the abaxial leaf surface of the host plant. Results of linear regression indicated that the lower developmental threshold temperature was $13.9^{\circ}C$ and the effective accumulated temperature was 526.3 DD. Data from a pepper field in 2015 indicated that overwintering adults first appeared during late June (daily average temperature = $25.7^{\circ}C$), reaching maximum density by early September. A choice test examining colonization preferences using a net cage and a Y-tube olfactometer revealed that females gravitated toward conspecifics (other females, males, or both), whereas males moved toward empty areas. Finally, we found that communal breeding results in a longer developmental period and higher eclosion rates than solitary breeding. Developmental periods and eclosion rates were also for colonies in a large space than for those in a small space. This outcome suggests that colonization effects on insect development are stronger in a smaller area.

• Journal of Aquaculture
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• v.1 no.2
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• pp.135-143
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• 1988

Rotifers became an important live food for fish larvae, especially for marine fishes, and Chlorella has been used as a very useful food for the mass culture of rotifer. However, not many tests for suitable Chorella species for the mass culture of rotifer were done and many of Chlorella sp. have been used without consideration of species for this purpose. There-fore, two species of marine Chlorella and four species of freshwater Chlorella were tested to select suitable species for the mass culture of a rotifer, Brachionus plicatilis. These Chlorella species were cultured in five different culture media; f/2, Erdschreiber, Complesal for marine species and S$\cdot$K, Wai and Complesal for freshwater species. Proximate analyses were done to see the protein, lipid and ash contents of a marine species, C. ellipsoidea and a freshwater species, C. vulgaris. Amino acids content of these species were also tested. C. ellipsoidea and C. vulgaris showed better growth than the other species. For marine Chlorella sp., f/2 media was better than Erdschreiber and Complesal. But for the freshwater species, Complesal showed the best result in growth. By the proximate analyses, C. ellipsoidea has higher lipid contents whereas C. vulgaris has higher protein and ash. In the analysis of amino acid of Chlorella, it was remarkable that freshwater Chlorella, C. vulgaris, has high content of $NH_3$ comparing with marine Chlorella, C, ellipsoidea. According to the above results, C. vulgaris seems have higher possibilities for mass culture of rotifer but further studies are needed.

• Journal of Veterinary Clinics
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• v.11 no.1
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• pp.343-346
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• 1994

Microsporum gypseum was identified as the prime cause of dermatitis in two young horses who were housed in unhygienic stables. The lesions were mainly distributed on the thorax, abdomen and rump. The diagnosis was established on the direct demonstration of dermatophyte in the cutaneous lesions and isolation of the fungus in pure and heavy growth from the infected hairs and skin scales on mycological medium at 3$0^{\circ}C$. Microscopoc morphology of the isolate in 'PHOL' stain many macroconidia and few microconidia. Epidemoilogical investigation revealed the prevalence of M. gypseum in the soil of stables. Mycological examination is highly imperative to distinguish the disease from other dermatological disorders. This apperas to be the first report of equine dermatitis due to M. gypseum in Westenn India.

• Journal of Mushroom
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• v.15 no.4
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• pp.223-228
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• 2017

This study was conducted to investigate the effects of fermented spent mushroom substrates (F-SMS) of Flammulina velutipes on growth performance, carcass traits, and economic characteristics of Hanwoo steers. A yeast strain (Saccharomyces sp. UJ14) and Bacillus strain (Bacillus sp. UJ03) isolated from fresh spent mushroom substrates of Flammulina velutipes were used as probiotics to prepare F-SMS. Twenty-four Hanwoo steers (14 months old) were allocated to three dietary treatments via a randomized block design and were slaughtered at 30 months of age. These treatment groups included Control (TMR), T1 (TMR containing 10% of F-SMS) group, and T2 (TMR containing 30% of F-SMS). Body weight gain was not influenced by the experimental diets. DM and TDN intakes in the finishing period were significantly (p < 0.05) greater in group T1 than in other groups. CP intake was significantly (p < 0.05) greater in group T2 than in other groups during the whole experimental period. Among carcass traits, rib-eye area and back fat thickness tended to increase with F-SMS supplementation. The appearance rate (%) of a meat yield more than grade A was the highest in group T1. The net profits increased by 1.2% and 13.3% in groups T1 and T2, respectively. In conclusion, if a proper feeding program (including feed safety) can be ensured, spent mushroom substrates of Flammulina velutipes can prove to be a highly profitable feed source for Hanwoo steers.

• Korean journal of applied entomology
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• v.57 no.2
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• pp.97-104
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• 2018

The larva of the white-spotted flower chafer, Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae), is known to have important medicinal properties, such as anti-cancer activity. However, the consumer market for the larvae is depressed because of its high production cost which needs to be reduced. This study was carried out to evaluate two spent mushroom substrates as food source for chafer larvae to improve the productivity and reduce production cost. The larvae were fed with spent substrates of king oyster mushroom (Pleurotus eryngii) (P-SMS) and shiitake mushroom (Lentinula edodes) (L-SMS), with a control of fermented oak sawdust. Effects of the diets on the development of P. brevitarsis seulensis and contents of minor nutrient components in the larvae produced were examined. In the P-SMS diet, the larval rearing period was shorter at 16.2 days, while the rate of larval weight gain and the cocoon weight were higher at 156.3% and 4.1 g, respectively, than that in the L-SMS diet. The pupation rate was 100% and the adult emergence was higher at 93.3% in the P-SMS diet than in the L-SMS diet with no difference to the control diet. In the P-SMS diet, the total nitrogen content during rearing was higher at 10.28% and the minor nutrient component, particularly Fe (145.8 mg/kg), was higher than that in other diets. The oviposition preference of adults showed no significant difference among the diets. Economic analysis showed that P-SMS costs approximately 667,960 Won less per 100 kg of larvae produced, compared to that of the control diet.

• Korean Journal of Food Science and Technology
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• v.10 no.2
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• pp.237-244
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• 1978

Among the strains isolated form the various sources, the strain AC-12 producing a powerful pectinase was selected by the extensive screening test. The selected strain was indentified and its toxicity investigated. The conditions of the pectinase production, the characteristics of the purified enzyme and the clarification effect on the apple juice were studied. 1. The selected strain AC-12 was identified by the classification method of paper and fennel and named as Aspergillus sp. AC-12. 2. As a result of the breeding test of the white mouse, no toxicity was found from this enzyme. 3. The yield of pectinase in the medium of defatted rice bran was much better than that in the medium of wheat bran. 4. The optimum conditions for the culture of the strain in the medium of defatted rice bran were that the cultural time was 72hrs, the amount of water to be added about 80%, temperature $30{\sim}35^{\circ}C$ and pH $3.0{\sim}5.0$. 5. The yield of pectinase was slightly increased by the addition of pectin to the medium of defatted rice bran and by the addition of pectin, $NaNO_3$ and $K_2HPO_4$ to the medium of wheat bran, respectively. 6. The optimum conditions for the enzyme activity were pH $3.0{\sim}4.0$ and temperature $40{\sim}50^{\circ}C$. The enzyme was stable below $40^{\circ}C$ and pH $2.0{\sim}8.0$, respectively. But above $50^{\circ}C,$ this enzyme was abruptly inactivated. The activity was slightly increased by the addition of $MnSO_4\;and\;CuSO_4.$ 7. It was regarded that the opimum temperature for the clarification of the apple juice was $40{\sim}50^{\circ}C$, the optimum pH 3.0 and the optimun concentration of the enzyme 0.1%, and the apple juice was almost clarified by the reaction at $45^{\circ}C$ for 60 minutes.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 1998.05a
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• pp.37-54
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• 1998

This study was conducted to investigate the effects of mugwort extracts on the blood ethanol concentration, liver function and low level of cadmuim(Cd) in rats. The effects of mugwort extracts on the blood ethanol concentration was studied in Sprague-Dawley rats (10 weeks old) administered p.o. with 25% ethanol (5g/1kg body weight) and then injected with mugwort extracts (at the 2% levels of daily feed consumption compared with the concentration of catechins level in mugwort extracts) in caudal vein. SD rats were divided into five groups : control group (CON-E, only ethanol and 0.85% saline sol'n treated instead of each extracts), water extracts of mugwort treated to the control (MDW-E), ethanol extracts of mugwort treated to the control (POH-E). And then rat plasma of each time (0hr, 1hr, 2hr, 3hr) was investigated ethanol concentration by gas chromatography. Another rats were measured at the time of 0 and 5hr for the test of GOD(Glutamic Oxaloacetic Transaminase) and GPT(Glutamic Pyruvic Transaminase). Components of each extracts were analyzed by using high performance liquid chromatography. The effects of mugwort extracts on the liver function were studied in culture of rat hepatocyte composed of three groups : Control group and two groups treated with each extracts (1% & 2% MDW, 1% & 2% MOH). Condition of rat hepatocytes cultured for 36hr at $37^{\circ}C$(5% $CO_2$ incubator), number of cells, GOT and GPT activity were investigated. The results obtained were summarized as follows ; 1. Catechins level of mugwort extracts was $8{\sim}10mg/100g(MDW)$, $3{\sim}4mg/100g(MOH)$ 2. The contents of (-)-Epigallocatechin was high in MDW 3. The effects of mugwort extracts on the blood ethanol concentration were as follows; 1) The order in ethanol degradation efficiency was MDW-E > MOH-E > CON-E. 2) Ethanol concentration significantly decreased (p<0.05) in MDW-E and MOH-E. 4. The effects of mugwort extracts on the liver function were as follows; (rat hepatocytes cultured for 36hr at $37^{\circ}C$) 1) Cells condition of MDW-L was better than other groups. 2) The order in number of cells (rat hepatocytes) was 2% MDW-L >1% MDW-L >1% MOH-L > Con-L > 2% MOH-L 5. Cd treatment increased concentrations of hepatic GSH level, and decreased GOT activity in plasma. Therefore, this results suggest that the effects of mugwort extracts may an important rols in degradation ethanol and recovery liver function in body. Also, Mugwort extracts may modify the toxicities of Cd in Cd-treated rats and play an important roles in preventing the liver from various toxicants including Cd in Cd treated rats.

• Korean Journal of Veterinary Research
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• v.22 no.2
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• pp.175-185
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• 1982

Since bovine lymphosarcoma causes considerable economic loss to the dairy industry, seroepidemiological survey on bovine leucosis virus (BLV) was carried out for the dairy herds throughout the country to observe the epidemiological situation of the disease by using immunodiffusion test. Attempts were simultaneously made to detect bovine leucosis virus in the lymphocytes from BLV antibody-positive cattle by means of fluorescent antibody techniques, syncytium assay and electron microscopy. In immunodiffusion test for BLV antibody in 2003 heads of dairy cattle selected randomly from 164 herds, the prevalence of positive reactors by regions were 37.8% in Central, 27.2% in Honam (Southwest), 28.0% in Youngnam (Southeast) and 25.2% in Youngdong (East coast)and averaging 29.7%. By provinces, Chungcheong appeared the highest prevalence of BLV antibody carriers (41.8%), while Jeonbug revealed the lowest incidence rate (24.4%). When the results of serological studies were analyzed by age groups and the sizes of herds, the number of reactors increased gradually with the advance in the age of cattle and the herd size. The highest rate of BLV carriers was found in the ages between 6 and 8 years, and in the size of herds with 20 to 50 heads. One hundred and seventeen breeding bulls from the central regions were tested for BLV antibody. Four out of 70 bulls (5.7%) of Korean cattle and 14 out of 39 bulls (35.9%) of Holstein were reactive for BLV antigens. Of 164 dairy herds examined, 17 herds (10.4%) have no BLV antibody-positive cattle, while 42 herds (25.6%) were included in the range of 20 to 40% of the positive rate and 10 herds (6.1%) in the range of over 80% of the rate. When the lymphocytes from the BLV antibody carrying cattle were cultured in the presence of phytohemagglutinin and stained with FITC-conjugated sheep anti-BLV serum, 8 out of 11 cases (72.7%) of BLV positive cattle revealed specific fluorescence for BLV in the lymphocytes. In syncytium assay of the peripheral lymphocytes of the cattle, 5 out of 7 (71.4%) lymphocytes from BLV antibody carriers induced syncytia in the indicators of bovine embryonic splenic cells. The cultured lymphocytes were examined with an electron microscope to detect the BLV particles. Two out of 6 specimens (33.3%) from the reactors showed the typical type C virus with the size of 90 to 110 nm around microvilli and in intracytoplasmic vacuoles.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.5
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• pp.465-476
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• 1993

Rockfish, Sebastes inermis is a ovoviviparous fish distributed around the coastal waters of Korea and Japan. Naturally beared larvae obtained from female fish kept in the aquarium were reared for three months in the laboratory. The naturally healed larvae were 6.05mm in average total length(TL) with $8+18{\sim}19=26{\sim}27$ myomeres. Melanophores were distributed on the top of head, around the intestine tube and on the dorsal and ventral margins of the tail, but none on the pectoral fins. The larva(7.1mm in TL) consumed all the yolk and oil globule, in 7 days after bearing, and jaw bones were more rapidly ossified than vertebrae and cranium. In 21 days after bearing, the larvae averaged 8.50mm in TL and the caudal notochord flex $45^{\circ}$. Fin development in S. inermis larvae proceeds in the following sequence: caudal, pectoral, dorsal, anal and ventral fin. Individuals $17.6{\sim}20.8mm$ in TL($65{\sim}69$ days after bearing) are regarded as the juvenile stage. All fins bones was nearly completed in this stage.