• The Korean Journal of Pesticide Science
• /
• v.12 no.2
• /
• pp.155-161
• /
• 2008

The inhibitory effect of herbal extracts using methanol and hexane collected from Sudan and Korea was evaluated on egg hatching of Meloidogyne incognita. The concentration of herbal extracts were 10,000, 1,000 and 100 ppm, respectively. They were treated to Meloidogyne incognita eggs after 3, 7, and 21 days of hatching. All herbal extracts inhibited egg hatching significantly compared to the control. The degree of inhibition was directly related to the concentration of the extracts. At 10,000 ppm, very fewer individuals were hatched at all the periods of exposure with the exception of the extract of fruits of Quisqalis indica which gave 84.4 and 54.5% at 7 and 21 days, respectively. Egg hatching was completely ceased, especially after 7 days for most of the extracts. While at 1,000 ppm relatively high numbers of eggs were hatched compared to the higher concentration, as well the extracts Q. indica reported many number of hatched eggs compared to others, especially at 21 days. On the other hand, the extract from pods of Cucumis mello var agrestis gave the least hatching number of eggs at all periods of exposure which gave 2, 8, and 3% at 3, 7, and 21 days, respectively in comparison to the control. Whereas at 100 ppm, inhibition increased with exposure time. However, the extract leaf of Desmodium caudatum represented the fewer hatching eggs at 3 and 7 days.

• Korean journal of applied entomology
• /
• v.45 no.3 s.144
• /
• pp.347-355
• /
• 2006

Possible nematicidal effects of plant extracts of 25 species uninfected by M. hapla were observed at the 5 times dilutions in all treatments and at the 10 times dilutions in Anemarrhena asphodeloides, Acorus calamus, Achyranthes japonica, Agrimonia pilosa, Dianthus chinensis, Geum aleppicum, Houttuynia cordate, Rudbeckia bicolor, Ricinus communis, Scrophularia buergeriana, Sesamum iindicum, Sedum kamtschaticum, and Sanguisorba officinalis. The 13 species plant extracts of 5 times dilutions were evaluated for the suppression effects on reducing densities of M. hapla by treating to C. lanceolata sown and transplanted later in pots. All the plant extracts showed suppressive effects on M. hapla except for.A. pilosa. The suppressive effects of A. asphodeloides, A. japonica, A. calamus, D. chinensis, R. communis, and S. buergeriana were over 80%. When the selected plants had been incorporated into the soil before C. lanceolata was sown, the numbers of root galls, egg sacs and $J_{2}$ appeared lower in the treatment of 12 plant species than in control except for S. indicum. But the suppressive effects were lower than the effects of selected plants being cultivated simultaneously in the field. A. calamus and A. japonica exhibited over 70% suppressive effects, among the tested plants.

• The Korean Journal of Pesticide Science
• /
• v.9 no.3
• /
• pp.268-273
• /
• 2005

The cultivating agroproducts are damaged by the Meloidogyne spp. which are gradually increasing in farm land soil. No effective control method for Meloidogyne spp., however, is available. Pasteuria penetrans which is one of the microorganisms in soil is used for biological control of Meloidogyne spp. although the method of mass production is limited. This study was conducted to investigate attachment and mass production effect of P. penetrans to M. arenaria under different temperatures (10, 30, 50 and $70^{\circ}C$) and pH values (4, 7 and 10). Attachment rates under these temperature and pH were more than 96% and 80%, respectively. In mass production rates, the number of P. penetrans attached on M. arenaria under different temperatures and pH were highly increased in root of tomato but not significantly different. Therefore, we concluded that P. penetrans can survive and attach on M. arenaria under various conditions. This method for mass production of P. penetrans can be provided to develop environmentally-friendly nematicide.

• Korean journal of applied entomology
• /
• v.27 no.1
• /
• pp.1-5
• /
• 1988

This study was carried out to find out the possibility of suppressing the root-knot nematodes (Meloidogyne spp.) of vegetable crops in vinyl house by soil solarization between crop seasons at Suweon in August, 1986. Soils in vinyl house were sterilized by polyethylene film of 0.03 mm thick with and without inner tunnel within a house(single and double layer solarization), and soil temperatures and nematode densities at 5, 15 and 30cm soil depth were recorded. Rootknot nematodes in soil contained in a vinyl bag were completely killed within 48hr at $40^{\circ}C$ in an incubator. With double layer treatment, the highest temperatures were $48.7^{\circ}C$ at 15cm and $45.2^{\circ}C$ at 30cm soil depth when outdoor temperature was $30^{\circ}C$, and the lethal temperature above $40^{\circ}C$ was recorded for 17 days with 7hr per day at 5cm soil depth from Aug 1 to Aug 31. Due to the increased temperature, root-knot nematode densitiy was suprressed 96,74 and 54% with sigle layer solarization and 100,99 and 98% with double layer solarization at 5,15 and 30cm soil depth, respectively. It is concluded that double layer solarization during hot summer will provide a sufficient level of suppresson on root-knot nematodes remnant in the soil of vegetable crops in vinyl house.

• Research in Plant Disease
• /
• v.20 no.1
• /
• pp.21-24
• /
• 2014

Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific primer set could be useful for rapid and accurate detection of P. brassicae.

• Korean journal of applied entomology
• /
• v.37 no.1
• /
• pp.65-71
• /
• 1998

Survey was conducted on the kinds and densities of pests associated with stored garlic collected from farms of major garlic production areas from 1994 to 1995 in Korea. Aceria tulipae, Rhizoglyphus sp., Ditylenchus dipsaci, and Tyrophagus putrescentiae were frequently detected with high densities and Tarsonemus bilobatus and Aphelenchus avenue showed relatively low detection rates and densities. Detection rate of A. tulipue was 38% in 1994, but the rate was 65% in 1995. Number of the mite ranged from 1 to 4,500 per scale. Detection rate of Rhizoglyphus sp. was 63% in 1994, but the rate was 13% in 1995 and average number of the mite ranged from 1 to 135 per scale in 1994. Garlics damaged by Rhizoglyphus sp. showed decaying symptom. T~rophagusp utrescentiae was detected from 22 farms among 32 farms surveyed in 1994 and from 21 farms among 39 farms surveyed in 1995. However, number of the mite on garlic scale was relatively lower than the other mites and its damages on stored garlic was not determined.

• Proceedings of the Korean Society of Soil Zoology Conference
• /
• 2001.10a
• /
• pp.13-13
• /
• 2001

• The Bimonthly Magazine for Agrochemicals and Plant Protection
• /
• v.9 no.6
• /
• pp.79-87
• /
• 1988

• Agrochemical news magazine
• /
• v.19 no.4 s.145
• /
• pp.22-23
• /
• 1998

• Agrochemical news magazine
• /
• v.25 no.7 s.200
• /
• pp.18-21
• /
• 2004