• The Korean Journal of Pesticide Science
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• v.12 no.2
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• pp.103-110
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• 2008

To develop the third generation herbicidal cyclic imide (Cyl) derivatives, the new 1-(4-chloro-2-fluoro-5-propargyloxyphenyl)-3-thiourea derivatives were synthesized and measured their herbicidal activities ($pI_{50}$) in vivo (preemergence) against rice plant (Orysa Sativa) and barnyard grass (Echinochlor crus-galli). The synthetic yields (%) of aryl derivatives (21-40) in general was higher than that of alkyl derivatives (1-20). In case of alkyl derivatives, the synthetic yield depended on the structural forms of alkyl amine groups. From the results of correlation analysis between herbicidal activities and substituents, the compound 8 and 24 showed the highest herbicidal activity against the shoot and root of barnyard grass. Especially, the compounds 11 and 6 showed the selective herbicidal activities between rice plant and barnyard grass.

• Journal of Korean Society of Forest Science
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• v.90 no.5
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• pp.600-607
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• 2001

This study was conducted to estimate the capability of Populus alba ${\times}$ glandulosa and Betula schmidtii for the uptake of Pb from the lead-contaminated soil and their tolerance to lead. Rooted cuttings in the former species and germinated seedlings in the latter species were planted in pots and irrigated with Pb-containing water for 60 days. In both tree species, growth inhibition was observed in 800, and 1,500ppm of $Pb(NO_3)_2$. Most Pb was accumulated in plant roots and only a small portion was transported to the shoots. The translocation rates of Pb for B. schmidtii and P. alba ${\times}$ glandulosa were 1.6-2.6% and 1.2-1.6%, respectively. The maximum Pb content accumulated in shoots was 468.0mg/kg d.w. in P. alba ${\times}$ glandulosa, and 602.0mg/kg d.w. in B. schmidtii. Although tolerance to lead was generally higher in B. schmidtii than P. alba ${\times}$ glandulosa, the highest tolerance to lead was observed in P. alba ${\times}$ glandulosa clone, 72-16. Finally, we discussed the applicability of P. alba ${\times}$ glandulosa and B. schmidtii for phytoextraction based on their Pb uptake ability, high biomass production, and easiness in large-scale cultivation.

• Korean Journal of Weed Science
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• v.7 no.1
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• pp.98-106
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• 1987

The effect of carbofuran (2, 3-Dihydro-2,2-dimethyl-benzofuran-7-ylmethyl carbamate) on rice growth was evaluated as a direct growth stimulant of rice. For this, several laboratory and field trials conducted from 1981 to 1986 at the Yeongnam Crop Experiment Station. Carbofuran solution affected the germination of rice seed. The growth of seminal roots was adversely affected by the increase of carbofuran concentrations while the length of single root became longer with the concentration increment up to 50 ppm. Carbofuran application (0.18g ai/$m^2$) at the rice nurserybed significantly enhanced the rice growth and recovered from the Low temperature damage. The enhancement effect was more pronounced at the plot that applied carbofuran before rice seeding as soil incorporation than top-dressing. The effect of growth enhancement further extended to transplanted lowland rice. This effect was greater at double cropping area (late of June transplanting) compared to single cropping area (May transplanting). Among important agronomic traits, the increment of panicle number was the most important direct effect for increasing rice grain yield by carbofuran application. Carbofuran application also exhibited the reducing effect against low temperature damage at reductive division stage and at rice heading stage and against submergence damage at booting stage through enhancement of fertile grain ratio, ripening ratio or photosynthetic activity.

• The Korean Journal of Mycology
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• v.33 no.1
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• pp.11-17
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• 2005

To increase the fibrinolytic activity and production of mycelium, extracts of 7 plant species were supplemented to the growth media of Armillaria mellea, and mycelial growth and enzymatic activity in the mycelium extracts of A. mellea were estimated. The mycelial production of A. mellea was slightly increased by adding ASH-R, UDVN or RGR extract, whereas KG extract significantly affected the growth. Supplement of ASH-S, UDVN and RGR extracts increased proteolytic activity from 36.8 to 46.1% Fibrinolytic activity was increased to $50{\sim}65%$ by supplement with RVS, ASH-S and RGR extracts, respectively. Enzyme extracts of the fungus grown with RGR extract supplement degraded all chains of fibrinogen within 2 hours, whereas control was required 3 hours. Degradation of fibrin fragments by the enzyme extracts was also observed through microscopy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.652-665
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• 1997

Allelopathic effects on some crops(rice, barley, wheat, Chinese cabbage, leaf mustard, onion, welsh onion, tobacco, red pepper) for extracts of Phytolacca americana and Armoracia rusticana were investigated in MS solid medium. Germination percentage, radicle length, hypocotyl length, plant height, fresh weight, dry weight, and seedling vigor were rapidly inhibited by methanol extract from Phytolacca americana according to increase of the concentration in test plants. Autotoxicity of Phytolacca americana began to appear at concentrations greater than 50$\mu$l methanol extracts per 20ml medium. The fresh weight and dry weight of Phytolacca americana were also decreased by its own methanol extract according to increase of the concentration. Initial amounts of growth of all crops were also inhibited by methanol extracts from Armoracia rusticana, but the inhibitory effects were lowered than those of methanol extracts of Phytolacca americana. Of four fractions, ethyl acetate, petroleum ether, $1^{st}H_2O, 2^{nd}H_2O$, reextracted from methanol extracts of Phytolacca americana, the ethyl acetate fraction showed the highest allelopathic effects on germination percentage and initial amounts of growth. Chlorophyll contents of rice, barley and Chinese cabbage were more inhibited in the ethyl acetate fraction than in the other fractions. Free proline content of Chinese cabbage was increased 31.2 times in the 100$\mu l$ ethyl acetate fraction, and the contents of rice and barley were also increased according to concentration levels in the ethyl acetate fraction.

• Applied Biological Chemistry
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• v.43 no.3
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• pp.184-189
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• 2000

Callus was induced from the petioles of scented-geranium (Pelargonium graveolense) in MS medium containing various concentrations of plant growth regulators. The highest frequency of more than 70% of callus was induced in 2 mg/l NAA and 0.5 mg/l BAP or 2 mg/l 2,4-D and 0.5 mg/l BAP combined treatment, while not in 2,4-D, NAA or BAP alone. When the callus was transferred to the MS medium containing 0.05 mg/l NAA and 0.5 mg/l BAP, were highest intensity of shoot formation, 14 shoots/callus, was induced after 5 weeks. The highest rooting was observed on hormone-free rooting media from the regenerated shoots after 3 weeks, indicating that the regeneration from geranium callus might be possible by changing the hormone ratios. Peroxidase (POD) specific activities in callus induced from 2 mg/l NAA and 0.5 mg/l BAP were higher than those of 2 mg/l 2,4-D and 0.5 mg/l BAP callus during the entire culture periods. POD isozyme C3 was the main cathodic POD isozyme expressed in NAA and BAP callus, while C1 was the main in 2,4-D and BAP callus. However, anodic POD isozymes, A1, A2 and A3 were expressed with similar activities in both hormone combinations.

• Korean Journal of Weed Science
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• v.8 no.1
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• pp.45-52
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• 1988

A serial study on differential response in absorption and translocation of $^{14}C$-bensulfuron-methyl was conducted by use of a group of selected rice cultivars as the tolerant or the susceptible to bensulfuron-methly. Trial 1. Differential Response in Absorption and Translocation of Selected Cultivar Group. The susceptible cultivar group has reached as higher rate as 102%, 113%, 115%, 127% and 113% of the tolerant cultivar group in root absorption per seedling, per unit dry weight, and the rate of translocation from bottom to shoot, respectively. Trial 2. Differential Response in Absorption and Translocation of Selected Rice Cultivar as Affected by Exposed Time of Root Portion upto 48 hrs. ${\bullet}$ Regardless of leaf stage of experimented plants, the amount of absorption per seedling and per unit dry weight has reached rather higher in the susceptible(cv. IR 1846) than the tolerant (cv. Chinsurah Boro II). However, separating by portions, the tolerant was realized higher rate of aborption in root but the susceptible in shoot, respectively. ${\bullet}$ Translocation rate from root to shoot, namely the individual seedling based rate of radioactivity in shoot to total radioactivity, was significantly higher in the susceptile than the tolerant. ${\bullet}$ Depending on higher rate of seedling growth at the time of chemical treatment, the susceptible (cv. IR 1846) was seemed more sensitive even at equivalent rate of absorption and translocation.

• Korean Journal of Weed Science
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• v.7 no.2
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• pp.220-235
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• 1987

The experiment was carried out to find the feasibility of using Oxyfluorfen in the paddy fields by investigating the difference of selective activity of Oxyfluorfen among rice cultivars and major paddy weed species. The dosage of Oxyfluorfen that show selective activity between rice cultivars and weed species ranged from 0.1 to 0.4kg ai/ha. The degree of growth inhibition was in order of whole-plant soaking application > root soaking application > stem bandage application, and in that case $10^{-5}$M Oxyfluorfen was treated after emergence. Especially the growth inhibition of rice cultivars and Cyperus serotinus was low, among others. Photosynthesis was severely inhibited at the Oxyfluorfen level above $10^{-4}$ M in all the tested weeds, but inhibition of respiration was not to be seen. Isolated single cells of two rice cultivars and Cyperus serotinus were tolerant to $10^{-5}$M Oxyfluorfen,but those of Echinochloa crus-galli and Sagittaria pygmaea were susceptible comparatively. The growth inhibition of suspension cultured rice cell induced by the increments of Oxyfluorfen concentration, and the degree of inhibition was higher in C.V. Mushakdanti than in C.V. Aichiasahi.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.61-67
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• 2001

Using mature seed-derived callus, optimal conditions for efficient callus growth and plant regeneration, and regeneration efficiency by callus type were investigated in zoysiagrass (Zoysia japonica steud.). Callus induction was highest when the seeds were cultured on MS medium containing 2 mg/L 2,4-D, 0.2 mg/L BAP, 4 mg/L thiamine-HCl and 100 mg/L $\alpha$-ketoglutaric acid. Callus growth was highest when callus were cultured on MS medium containing 0.5 mg/L 2,4-D, 0.05 mg/L BAP, 4 mg/L thiamine-HCl and 100 mg/L $\alpha$-ketoglutaric acid. Plant regeneration was highest when callus was transferred on MS medium containing 3% maltose and 1 mg/L BAP, or 1 mg/L thidiazuron (TDZ). The combinations and concentrations of 2,4-D and BAP were shown to be critical factors for both the frequency and the type of callus. And four morphologically distinct types of callus were induced from the 2,4-D and BAP treatment. Type I,II and III calli produced shoots upon subculture, while the watery callus, type IV produced roots without shoots. Of four types of callus, type I exhibited the maximum frequency (82%) of shoot regeneration and the minimum frequency (4%) of albinism.

• Korean Journal of Organic Agriculture
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• v.7 no.2
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• pp.179-188
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• 1999

The effects of earthworm casts as a plant growth media on the growth of orchardgrass seedlings and the changes of physico-chemical properties in worm casts mixture soils were investigated during the growth period of 1998. Worm casts were mixtured with vermiculite, perlite and peat moss, and mixture ratios of worm casts with commercial recommended soil were 100:0(control), 75:25, 50:50, 25:75, and 0:100, respectively. The results were obtained as follows; 1. Plant length(PL) was increased in higher mixture ratios of worm casts and peat moss than those of other mixture ratios of commercial recommended soils. 2. Number of tillers(NT) were significantly increased in mixture ratios of worm casts and peat moss(50:50) and 100% of vermiculite. 3. Root length(RL) was significantly differences between mixture ratios at the worm casts, and the highest value was obtained at all mixture ratios of worm casts and perlite. 4. Dry weight of leaf(LW) was not significantly differences at mixture ratios of 50:50 in all treatments. Especially, between the mixture ratios of worm casts with peatmoss(25:75) and peat moss(75:25) were not significantly differences in dry weight of leaf(LW). The highest value of dry weight of leaf(LW) was obtained at mixture ratios of peat moss and worm casts. 5. Dry weight of tillers(SW) was not significantly differences at mixture ratios of 50:50 in all treatments, and peat moss(75:25) and vermiculite(75:25), respectively. 6. biological yield(BY) at all treatments were increased by yield components of dry weight of tillers(SW), dry weight of root(RW) and length of root(RL). 7. The biological yield of orchardgrass seedlings was greatly increased with mixture ratios of worm casts and peat moss over the 50%.