• Proceedings of the KSCN Conference
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• 2004.05a
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• pp.434.1-434
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• 2004

본 연구는 비타민 C와 비타민 E의 보충 급여가 항산화 비타민 수준 및 항산화 체계에 미치는 영향에 대하여 알아보고자 하였으며, 경기지역 남자대학생 60명을 대상으로 조사하였고 항산화 비타민 보충 급여의 효과를 관찰하기 위해서 비타민 C 보충군, 비타민 E 보충군, 비타민 C와 E 보충군으로 각 군별 당 10명씩 나누어 2주간 보충 급여하였다. 비타민 급여량은 비타민 C 500 mg, 비타민 E 400 IU이었다.(중략)

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.387-396
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• 2004

This experiment was carried out to investigate the effects of dietary vitamin C and E on carcass characteristics of Hanwoo steers. Thirty seven Hanwoo steers were randomly assigned to one of four treatments. The four treatments were control(vitamin E 20 IU/kg feed), vitamin C(vitamin C 0.1%/kg feed and vitamin E 20 IU/kg feed), vitamin E(vitamin E 220 IU/kg feed) and vitamin C plus E(vitamin C 0.1%/kg feed and vitamin E 220 IU/kg feed). Hanwoo steers were fed experimental diets for 3 months of the last finishing period. Animals fed the diet containing vitamin C plus E had higher(P<0.05) yield grade than those fed the other diets but quality grade was not different between animals fed the control diet and vitamin-supplemented diets. Proximate analysis in longissimus dorsi was not different between animals fed the control diet and vitamin-supplemented diets. Vitamin C concentration in longissimus dorsi was higher (P<0.05) in the animals fed the vitamin C diet and the vitamin C plus E diet than in those fed the control diet and the vitamin E diet. Vitamin E concentration in longissimus dorsi was higher(P<0.05) in the animals fed the diet supplemented with vitamin E and the diet supplemented with vitamin C plus E than in those fed the control diet and the vitamin C diet. Animals fed the diet supplemented with vitamin C was higher(P<0.05) in myristic acid, palmitic acid, palmitoleic acid and oleic acid in the longissimus dorsi than those fed the other diets, but linoleic acid in longissimus dorsi was higher(P<0.05) in the animals fed the vitamin E diet than those fed the other diets. However, saturated fatty acid and unsaturated fatty acid were not different between animals fed the control diet and vitamin-supplemented diets.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.05a
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• pp.49-59
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• 2003

Heat stress환경의 육용종계에서 사료에 첨가한 비타민 C(200 mg/kg)와 비타민 E(250 mg/kg)가 난각 품질 및 경골 강도에 미치는 영향을 확인하고자 실험을 실시하였다. 강제 환우에서 회복된 83주령의 Ross품종 육용종계 160수를 4처리 4반복 10수씩 개별 케이지에 수용 한 뒤, 10일간의 적응 기간을 둔 뒤, 3주간에 걸쳐 32$^{\circ}C$에서 지속적으로 온도를 유지하면서 사료 섭취량, 산란율, 폐사율, 난중, Haugh unit, 난각의 품질, 경골 파괴강도 그리고 혈액 중의 혈구 세포 등을 조사하였다. Heat stress는 폐사율, 산란율, 난중, 그리고 Haugh unit를 감소시키는 경향이 있었으나 통계적으로 유의하지는 않았다. 난각의 SWUSA와 압축 파괴강도는 비타민 C/E 첨가구에서 2, 3주차에 무첨가구보다 유의하게 높았으나 (P<0.05), 비타 C 또는 E 첨가구 사이에서는 유의차가 없었다. Heterophil과 lymphocyte의 숫자는 heat stress 동안에 각각 증가 또는 감소하였다. 처리구간에는 비타민 C/E 첨가구가 H/L/ratio 수준이 가장 낮았으며, 무첨가구가 가장 높은 경향을 나타내었다. 경골의 파괴강도는 비타민 C 첨가구에서 가장 높았다(P<0.05). 혈액 중의 비타민 C 농도는 비타민 C첨가구와 비타민 C/E 첨가구에서 각각 12.73 g/ml과 8.23 g/ml으로 높았다(P<0.05). 비타민 E 첨가구와 비타민 C/E 첨가구에서 혈액 중 비타민 E 농도가 유의하게 높았다 (P<0.05). Corticosterone 농도는 무첨가구에서 5.97g/ml으로 통계적으로 유의하게 높았다.(P<0.05).

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.4
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• pp.651-656
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• 2001

Ascorbic acid(AsA) is unevenly distributed throughout all body cells and fluids. Multiactivities of AsA in many biological systems and in various scientific fields were reported. In this study we aimed to clarify the inhibitory action of high concentration of AsA on the cell growth in 3T6 fibroblasts. The cells wee exposed to AsA at various concentration. It showed that 3T6 fibroblasts wee dead by the medium which contained AsA at the concentration higher than 0.5 mM. AsA caused hydrogen peroxide ($H_2O$$_2$) generation in a concentration dependent manner. These results suggested that the $H_2O$$_2$ was formed in the medium by AsA and acted as a cytotoxic gent. Moreover, it is supposed that hydroxyl radical (.OH) induced from $H_2O$$_2$also acetd as actively cytotoxic agent. This lethal effect of AsA causing the cell death was inhibited by the addition of catalase in the medium. Therefore, addition of AsA at the normal concentrations stimulate cell growth, but excess concentrations of AsA induce cell death.

• Applied Biological Chemistry
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• v.43 no.1
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• pp.39-45
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• 2000

The stability of vitamin A, $B_1,\;B_2,\;B_6$, C in aqueous multivitamin solutions was carried out by means of estimation of reaction velocity and the results are described in this paper. The stability of vitamin A, $B_1$ and C due to thermal degradation method in aqueous multivitamin solutions was evaluated at 40, 50, 60 and $70^{\circ}C$ up to 40 days. The shelf-lives of vitamin A, B₁ and C in this preparation, calculated using the Arrhenius equation, were 1493, 449 and 639 days at $25^{\circ}C$ respectively. Examination was made on the effect of initial concentration of vitamin $B_2$$(C_0)$ on light fading of vitamin $B_2$ in aqueous multivitamin solutions and it was found that the fading progressed according to the following formula : $-{\frac {dc}{dt}}=K_c\;{\frac C{C_0}}$ where Kc is apparent light-fading rate constant relate to $C_0$. Photodecomposition of vitamin $B_6$ in aqueous multivitamin solutions was apparently first order kinetics and was stable in polyethylene>brown color>glass container to sunlight. Photodecomposition of vitamin $B_6$ in four seasons also investigated.

• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.635-644
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• 2004

This experiment was earned out to investigate the effects of dietary vitamin C and E on lipid oxidation and stability of color in Hanwoo steer beef. Thirty seven Hanwoo steers were randomly assigned to one of four treatments. The four treatments were control(vitamin E 20IU/kg feed), vitamin C(vitamin C 0.l%/kg feed and vitamin E 20IU/kg feed), vitamin E(vitamin E 220IU/kg feed) and vitamin C plus E(vitamin C 0.l%/kg feed and vitamin E 20IU/kg feed). Hanwoo steers were fed the experimental diets for 3 months at the last finishing period and pH, color, TBARS, concentration of myoglobin, water holding capacity, reduction by heat, drip loss and purge loss of longissimus dorsi were analyzed at 1, 3, 5, 7 and 10th day of storage, respectively. pH value, Hue angle, metmyoglobin concentration, TBARS, drip loss and purge loss in longissimus dorsi were increased as the storage period elapsed. However, water holding capacity in longissimus dorsi was decreased as the storage period elapsed. The animals fed the vitamin C plus E diet were higher in pH value and oxymyoglobin concentration, and lower in metmyoglobin concentration than in those fed the other diets during the whole storage period and lowest in TBARS. TBARS and Hue angle in longissimus dorsi were 10wer(P<0.05) in the animals fed the vitamin-supplemented diets than in those fed the control diet. Reduction by heat in longissimus dorsi was higher(P<0.05) in the animals fed the diets supplemented with vitamin E or vitamin C plus E than in those fed the control diet or vitamin C-supplemented diet. According to these results, it may be concluded that feeding the diets containing 0.1% vitamin C or 220IU vitamin E per kg feed or the diet mixed 0.1% vitamin C with 220IU vitamin E to Hanwoo steers can retard lipid oxidation of longissimus dorsi and stabilize beef color.

• Food Science and Preservation
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• v.17 no.4
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• pp.451-456
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• 2010

We investigated changes in the quality of pulp-containing apple juice, during storage, after addition of various amounts of vitamin C, which was stable over time. Neither sugar content nor acidity level varied when vitamin C was added. The pH was slightly lower (pH 4.29-4.30) in juice with added vitamin C than in unsupplemented juice (pH 4.40). The L and b color values fell as vitamin C content rose and the storage period was extended. In sensory evaluation tests, taste and overall acceptability were higher for juice to which vitamin C had been added to 0.02% (w/v) than for unsupplemented juice. Vitamin C levels fell less during storage at $4^{\circ}C$ than at higher temperatures. The alcohol-soluble color (ASC) value fell as the amount of added vitamin C rose, and tended to be lower when juice was stored at $4^{\circ}C$ compared to $37^{\circ}C$. In summary, apple juice containing pulp was optimally stored at $4^{\circ}C$ after addition of 0.02% (w/v) vitamin C

• Journal of the Society of Cosmetic Scientists of Korea
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• v.29 no.1
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• pp.169-184
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• 2003

비타민 C(L-ascorbic acid)는 강한 항산화성, 피부에 대한 높은 안전성으로 인해 피부의 노화방지, 미백, 주름 개선 등의 기능성 화장품 원료로써 많은 관심이 있는 물질이지만, 화학적으로 매우 불안정하여 쉽게 산화, 분해되므로 화장품 제형으로 포함시키는데 곤란한 문제가 있다. 본 연구에서는 비타민 C 의 안정성에 대한 단점을 보완하기 위하여 생체 및 피부 친화성이 우수한 무기물을 사용하여 비타민 C 를 캡슐화(encapsulation)한 분말상의 유-무기 복합물질 Vitabrid-C 를 합성하고, 이를 함유하는 피부 미백용 트윈케익 파우더를 개발하였다. 우선 Vitabrid-C 는 수용액상에서 비타민 C 를 수화된 산화아연(ZnO)으로 1 차 캡슐화하여 나노입자를 형성시키고, 그 표면을 실리카(silica)나노 입자로 한번 더 코팅하여 표면의 껍질이 다공특성을 갖는 분말을 제조함으로써 완성하였다. 이렇게 제조된 Vitabrid-C는 순수 비타민 C 에 비해 우수한 안정성을 보였으며, 캡슐 내 비타민 C 가 서서히 방출되는 서방특성을 발휘하였다. 또한 Vitabrid-C는 입자의 크기가 미세하고 균일하여 트윈케익 처방에 용이하게 적용할 수 있었다. Vitabrid-C와 순수 비타민 C의 생화학적 동등성에 대한 평가는 tyrosinase 억제능(L-DOPA oxidase 억제) 및 DPPH항산화 실험을 통하여 비교하였다. 트윈 케익 처방에 적용된 Vitabrid-C 에서 비타민 C 의 피부 투과경향을 Franz diffusion cell 법을 이용하여 확인하였다 또한 Vitabrid-C가 포함된 트윈케익을 건강한 피부를 가진 25 세 이상되는 여성의 전박에 색소 침착을 유도한 후 피부색 개선 효과 평가를 통해 임상적 효능을 평가하였다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.5
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• pp.643-650
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• 1996

A long term feeding trial was conducted to study the effects of different dietary vitamin C levels on growth and its tissue distributions in juvenile Korean rockfish, Sebastes schlegeli. Prior to the start of feeding trial, fish were fed the basal diet supplementing no L-ascorbic acid for four weeks to minimize their body reserves of vitamin C. Then fish were divided into six groups with triplicates and given one of the laboratory semipurified diets supplementing either 0, 25, 50, 75, 150, or 1500 mg L-ascovbic acid (AA)/kg diet $(C_0,\;C_{25},\;C_{50},\;C_{75},\;C_{150},\;&\;C_{1500})$. Fish fed the $C_0$ diet had lower percent weight gain, feed conversion ratio, specific growth rate, and protein efficiency ratio than did fish fed the other diets (P<0.05). After 28 weeks of feeding trial, tissue AA concentrations of fish fed $C_0$ diet were lower than those of fish fed $C_{1500}$ diet (P<0.05). A large amount of total tissue Ah may be reserved in muscle, but the unit AA concentration seemed to be higher in brain than did the other tissues. The growth performances of fish fed $C_{25}$ diet were not different compared to those of fish fed $C_{50}-C_{1500}$ diets (P>0.05), and diet analysis of vitamin C showed that the $C_{25}$ diet had 65 mg AA/kg diet. Therefore, the present long-term study may suggest that the dietary vitamin C requirement is approximately 65 mg AA/kg diet in juvenile Korean rockfish.

• Journal of Nutrition and Health
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• v.55 no.5
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• pp.523-532
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• 2022

Vitamin C is an important physiological antioxidant which neutralizes reactive oxygen species (ROS) and reduces the oxidative stress in the body. Although it has been associated with various diseases, few studies have reported the dose-response relationship between vitamin C intake, storage and functions in the body, including its antioxidant function. The criteria to establish the Dietary Reference Intakes for Koreans (KDRIs) for vitamin C were based on the changes in plasma concentrations and saturation of leukocytes according to intake levels and the effects on antioxidant capacity and risk of metabolic diseases. When establishing the 2020 vitamin C KDRI, while there was no change in the criteria from those of 2015, the reference values were recalculated and revised to reflect changes such as the new standard weight by age. As the number of people consuming dietary supplements has increased over the last decade, only about 10% of adults consume less than the average total vitamin C, but the proportion of adolescents and elderly who consume less than the average is high. On the other hand, as the intake of vitamin C supplements increases, the proportion of people consuming excessive vitamin C is also increasing. There is a body of opinion that it is necessary to establish a vitamin C KDRI for smokers or people with chronic diseases such as the metabolic syndrome, but these standards have not been established due to the lack of supporting scientific evidence. As a result, studies to establish vitamin C KDRI for Korean smokers and patients with the metabolic syndrome, as well as studies on the excessive intake of vitamin C due to supplementation and interactions with other nutrients, are needed.