• KSBB Journal
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• v.19 no.4
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• pp.274-283
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• 2004

Six lumbrokinase (LK) fractions from Lumbricus rubellus lysates were purified by a series of column chromatographies. The molecular weights of the six LK fractions appeared to range from 24.6 to 33.1 kDa. In the experimental model of rat venous thrombosis, the thrombus weight and PAI activity decreased significantly when the LK was administered orally. However, the activities of APTT, PT and plasmin showed a significant increase. The aggregation of rat platelets pretreated with various LK doses was inhibited by thrombin, and the MDA generation decreased. The rat thoracic aorta and mesentric arteries contracted with phenylephrine relaxed due to the treatment of the LK fractions. These results suggest that the fibrinolytic effects of LK were mediated not only by proteolytic activity, but also by the inhibition of platelet agregation and the relaxation of blood vessels. It is concluded that the LK may be useful as a hemolytic agent for treatment of fibrin clot.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.10
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• pp.1536-1544
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• 2005

We have investigated physiological effects of soy ice cream with oligosaccharide on oxidative stress and fecal microflora in streptozotocin-induced diabetic rats. Parched soybean powder (7.6$\%$, w/w) substituted skimmed milk and cream, soybean oil (7.6$\%$, w/w) for milk oil, and fructooligosaccharide (9.5$\%$, w/w) for sucrose. Five types of ice cream were prepared: regular, oligosaccharide-supplemented regular, soy, oligosaccharide - supplemented soy, and oligosaccharide - supplemented black soybean ice cream . Freeze - dried ice cream was supplemented to AIN93-based diets at 30$\%$ (w/w) containing 6.5$\%$ soy and 4.5$\%$ fructooligosaccharide. Diabetes was induced by intramuscular administration of streptozotocin, and experimental diets were given for 4 weeks. Plasma concentration of thiobarbituric acid reactive substances (TBARS) was significantly increased in the diabetic rats compared with the normal rats, then was significantly decreased with feeding soy ice cream containing diet compared with regular ice cream containing diet among the diabetic groups. The levels of TBARS in liver were decreased in the rats that were fed either soy or oligosaccharide ice cream compared with the rats that were fed regular ice cream. Erythrocyte superoxide dismutase activity was significantly increased in the rats fed soy ice cream compared with the rats fed regular ice cream. Erythrocyte glutathione peroxidase and catalase activities were significantly increased in the rats fed black soybean ice cream. Fecal concentrations of Lactobacilli were significantly higher in the rats fed soy ice cream and oligosaccharide- supplemented soy ice cream than that of the rats fed regular ice cream. Fecal concentrations of Bifidobacteria were significantly higher in the rats fed oligosaccharide- supplemented soy ice cream than that of the rats fed regular ice cream. In conclusion, oligosaccharide- supplemented soy ice cream suppressed lipid peroxidation and improved the got microbiota in diabetic rats compared with milk-based regular ice cream.

• Journal of Nutrition and Health
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• v.9 no.1
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• pp.99-103
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• 1976

농축생선단백질(Fish Protein Concentrate, FPC)로 보충된 국수의 영양가를 연구하기 위하여 25일된 쥐를 사용하여 4주 동안 동물 실험을 하였다. 각각 10마리씩 네 group으로 나누어 0, 10, 15% FPC와 표준 casein diets를 주고 매일 섭취량을 기록하고, 매주 동물의 체중을 재서 Protein Efficiency Ratio (PER)를 계산함으로써 단백질의 질(質)을 평가하였다. 4주 후에 동물의 간을 취하여 수분과 지방의 양을 측정하였다. 또한 농축생선 단백질로 보충된 국수가 음식으로서의 적응도를 측정하기 위하여 어른 10명과 아이들 5명에게 관능 검사를 실시하였다. FPC 15% diet group의 체중은 4주 동안에 157.2g이 증가했고, FPC 10%는 135.7g, casein은 103.0g, FPC 0%(plain noodle)은 25.8g 밖에 되지 않았다. 이것들의 PER은 FPC 15% diet group 이 3.83으로 제일 높았고, 다음이 FPC 10%로 3.35였고, casein은 이보다 낮은 3.31이였다. 간의 지방양은 FPC 15% diet group이 2.94%로 제일 낮았으며, FPC 10%가 3.98%, casein이 5.57%의 순서였다. 지방의 양이 적을수록 단백질의 질(質)이 높다는 것을 말하며, 5%정도가 보통이다. PER, 동물의 체중과 지방의 양으로 단백질의 질(質)을 측정한 결과 FPC 15% 국수가 질(質)이 제일 좋다는 것이 판명되었다. 관능검사 결과 전체적 계수는 FPC 15% 국수가 제일 낮은 2.7 (1.0에서 5.0까지 사용)이고 다음이 FPC 10% 국수로 3.5였으나 plain noodle만 못하였다. 특히 색갈(color)에 많은 차이를 보였기 때문이며, 맛(flavor)과 질(texture)에는 별 차이가 없었다. FPC 15% 국수는 색갈이 plain noodle에 비해 검은 편이였으나 모밀국수 보다는 색갈이 희므로 그렇게 문제가 될것 같지는 않다. 어린 아이들에게는 닭 국물에 국수를 넣어 주였더니 라면과 맛이 비슷해 아주 좋아했다.