• Title/Summary/Keyword: 비속성

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Delivery of Ti Plasmid into Nicotiana sanderae Protoplasts via Liposomes (Liposome을 이용한 Ti Plasmid의 꽃담배 원형질체내 도입)

  • Lim, Myung-Ho;Jeong, Jae-Dong;Kim, In-Soo
    • Applied Biological Chemistry
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    • v.37 no.5
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    • pp.343-348
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    • 1994
  • Ti plasmid of A. tumefaciens was labeled with $^3H-thymidine$, purified and encapsulated into phosphatidylserine (PS) and PS-cholesterol (Chol; 1 : 1 molar ratio) liposomes by lyophilization-rehydration method. PS was supplemented with 1 mole percent octadecyl rhodamine B for fluorometric measurement of PS. Liposomes entrapping $^3H-Ti plasmid$ were fused with Nicotiana sanderae protoplasts by treating with 5 mM $CaCl_2$ and 10% PEG. The fusion was evidenced by fluorescence microscopic technique. The amounts of Ti plasmid and PS associated with protoplasts were assayed by the radioactivity of $^3H-Ti plasmid$ and by the fluorescence of rhodamine B. About 7.9% of the PS liposome and 7.2% of PS-Chol liposome were fused with protoplasts. During the fusion process, about 30% of the liposomal contents of PS-Chol liposome was leaked, in contrast to about 60% leakage of its contents in PS liposome. Accounting the number of liposomes fused with protoplasts together with the encapsulation efficiency and the leakage of liposomal contents, it was calculated that ca. 1,700 Ti plasmid was transfered into one protoplast by the present method. This result may indicates that the present method transfers enough Ti plasmid into plant protoplast to elicit genetic transformation of plants.

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Basic Study on Bridge Asset Management Framework and LOS for Efficient Downtown Bridge Maintenance (효율적인 도심지 교량관리를 위한 교량자산관리 체계 및 서비스수준(LOS) 개발을 위한 기초연구)

  • Kim, Gyung-Hyun;Kim, Dong-Jin;Lim, Jong-Kwon;Park, Mi-Yun;Lee, Min-Jae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.5
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    • pp.671-679
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    • 2016
  • Bridges, which constitute one of the key facilities in a social infrastructure, are easily accessed and used by users, so that keeping their performance above a certain level is essential. According to various cases in the U.S., Japan and Europe with a long construction history, it is expected that the maintenance cost of bridges in Korea will increase continuously in the future, so a rational decision making system based on engineering factors is necessary to optimize the performance of and maintain them by allocating the limited budget efficiently. This study is a preliminary basic study for the purpose of developing a common asset management system for managing all of the bridges and maintaining the level of service provided by them. The scope of this preliminary study is limited to bridges in urban areas. The bridge asset management system for bridges in urban areas, their level of service (LOS) and performance measure (PM) were developed by carrying out a workshop consisting of both experts and stakeholders. The analysis on the weights of the value and performance measure for each performance indicator was carried out by using the multi-attribute utility theory and the AHP method. In order to confirm the application of the weight of the performance measure and value of the performance indicator, six bridges in an example city were selected, LOS analysis was applied and its results were reviewed.

Broadcasting and Caching Schemes for Location-dependent Queries in Urban Areas (도심환경에서 위치의존 질의를 위한 방송과 캐싱 기법)

  • Jung Il-dong;Yu Young-ho;Lee Jong-hwan;Kim Kyongsok
    • Journal of KIISE:Databases
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    • v.32 no.1
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    • pp.56-70
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    • 2005
  • The results of location-dependent queries(LDQ) generally depend on the current locations of query issuers. Many mechanisms, e.g. broadcast scheme, hoarding, or racking policy, have been developed to improve the system peformance and provide better services, which are specialized for LDQs. Considering geographical adjacency of data and characteristics oi target area, caching policy and broadcast scheme affect the overall performance in LDQ. For this reason, we propose both the caching policy and broadcast scheme, which these features are reflected in. Based on the adjacency of data in LDQ, our broadcast scheme use Hilbert curve to cluster data. Moreover, in order to develop the caching policy suitable for LDQ on urban area, we apply the moving distance of a MH(Mobile Host) to our caching policy We evaluate the performance of the caching policy measuring the workload of MHs and the correctness of LDQ results and the performance of the broadcast scheme measuring the average setup-time of MHs in our experiments. Finally, we expect that our caching policy Provides more correct answers when executing LDQ in focal cache and leads significant improvement of the performance of MHs. It also seems quite probable that our broadcast scheme leads improvement of battery life of the MH.

Design and Implementation of JBI Component Deployment tool Based on the open sources (오픈소스 기반의 JBI 컴포넌트배치도구의 설계 및 구현)

  • Park, Jae-Won;Choi, Jae-Hyun;Lee, Jae-Sung;Lee, Nam-Yong
    • The KIPS Transactions:PartD
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    • v.16D no.5
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    • pp.715-728
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    • 2009
  • The Enterprise Service Bus based on Java Business Integration is an web service standard and one of the methods for implementing distribution channels of Service Oriented Architecture. Consisting of open source group, extensive venders and users, the ESB based JBI has the problems of ineffectiveness as well as advantages of extensibility of service plug-in. That is, in case users need to use Service plug-in, manual connection of packaging process and sequential distribution method is required. This study, therefore, proposes as a way of trouble-shooting the user-oriented component deployment tool which can manage entire process for deploying The ESB middleware platform to Service unit. At the same time, this study elicited the requirements based on issues of JBI-based ESB and has developed the modeling property, packaging, distribution and evaluation thru Schema analysis of JBI-compatible component. Using the deployment tool this study proposed, users will be able to perform and manage the whole deploying process without additional manual work for connecting component. Not only it is expected that interface based on Graphic User Interface provide usability and convenience but they can also minimize the errors rate through component and route validity verification function provided in deployment tool.

A study on the Regional Informatization Policy Implementation System in Korea (한국 지역정보화 추진체계 개선에 관한 연구: 지능정보사회의 지역균형발전을 중심으로)

  • Jin, Sang-Ki
    • Informatization Policy
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    • v.24 no.3
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    • pp.67-90
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    • 2017
  • This study is conducted to find out problems in the implementation system of regional informatization policies in Korea and solutions to enhance the effectiveness in policy execution. The study uses research methodologies such as participant observation, interview and AHP for experts and employees of public organizations for regional informatization policies. Many implications were found in the analysis, including that policy relations, structure of the policy implementation system, and environment and contents of regional informatization policiesin Korea have to be innovated and re-organized. Especially, this paper emphasizes the horizontal and cooperative relationship between the central and local governments and redesigning of the legal system on the regional informatization. This paper also shows expectations on reshaping of the regional informatization policies with the paradigm shift of the government power toward decentralization. This paper also finds diverse views on the problems and solutions for the regional informatization policy implementation system based on different characteristics and interests of policy participants. This paper finds the possibility of applying the policy network model to regional informatization policy implementation, which can be supported by Rhodes & Marsh(1992)'s theory. Therefore, this paper shows the change of regional informatization policies can be expected through application of the policy network. Although the paper draws many academic and policy implications, they are limited to the implementation system of regional informatization policies only.

Characteristics of Manganese Nodule from the East Siberian Sea (동시베리아해 망간단괴의 특성)

  • Koo, Hyo Jin;Cho, Hyen Goo;Yoo, Chan Min;Jin, Young Keun
    • Journal of the Mineralogical Society of Korea
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    • v.30 no.4
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    • pp.219-227
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    • 2017
  • Manganese (Mn) nodules in the Arctic Sea have been founded in the Kara Sea and Barents Sea, but mineral and chemical compositions have been rarely investigated. In this study, mineralogical and geochemical characteristics of Mn nodules obtained during the Arctic Expedition ARA07C in northern East Siberian Sea were identified, and then genesis of Mn nodules were estimated by using these characteristics. Main manganese oxide minerals constituting the manganese nodule were buserite, birnessite, and vernadite. The Mn nodules generally represent radiated and massive texture, and the layered texture was developed restrictively. The radiated texture, main feature of the manganese nodule in the East Siberian Sea, is mainly composed of cuspate-globular microstructure. Compared with the Mn nodules in Pacific and Indian Oceans, Mn nodules of the East Siberian Sea are abundant in Mn, but Fe is too scarce. There was no difference in the chemical composition and microstructures between outer and inner part of nodule. Therefore, nodules are most likely to have only one genesis during their growth, and all of nodules indicate the diagenetic in $Mn-Fe-(Cu+Ni+Co){\times}10$ ternary diagram. It is considered that the manganese nodules in the East Siberian Sea are characterized by high Mn contents because manganese contents in the Arctic Ocean were mainly resulted from river or coastal erosion and most of them are trapped in the Arctic Ocean.

GIS spatial D/B formation of geothermal data and Distribution of Heat Flow of Korea (한국의 지열자료 GIS 공간 D/B 구축과 지열류량 분포)

  • Kim, Hyoung-Chan;Lee, Young-Min;Park, Jeong-Min
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.06a
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    • pp.459-460
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    • 2006
  • 현재 남한의 지열류량 측정값으로는 총 363개 지점의 자료가 측정 및 수집되어 있다. 이것은 Mizutani et at. (1970), 장정진 외(1970), 그리고 서정희(1976) 등의 자료, 총 35개의 자료도 추가된 것이다. 1989년 이후부터 측정된 지열류량 자료는 217개 자료이며(임정웅 외, 1989; 임정웅 외, 1996; Lim and Kim, 1997; 염병우 외, 1997), 모두 직접 측정한 것이나, 1989년 이후 보고된 지열류량 자료에 약간의 오류가 있어 이번 연구에서 수정 보완하였다. 또한 과거의 자료 35개 자료는 이미 지열류량 측정치가 논문화 되어 있는 것으로 암석시료는 없다 1989년 이후 2004년까지 자료 217개 2005년도 추가 자료 111개의 지열류량 자료는 암석시료도 있으며, 측정기기가 서로 달라 오차가 있을 수 있어 서로 보정을 해야 할 필요가 있어 시추공 주변 암석을 새로 수집해서 신장비로 다시 측정 보정하였다. 지열류량 D/B 구축은 각 자료의 일련번호, 고유번호 (Sn.), 위경도 좌표 (longitude, lattitude), 암석의 열전도도(thermal conductivity), 지온경사 (thermal gradient), 지열류량 (heat flow)등으로 구성되어 있다. 지열류량 자료 공간 데이터베이스는 점 속성을 가지며 자료형태는 각종 소프트웨어와 호환성이 좋은 shape 파일 형태로 작성하였다. 또만 최근 천부 토양 및 암석 열물성을 이용한 냉난방시스템 즉, Heat Pump System 설계를 위하여 반드시 들어가야 하는 요소인 열확산율, 공극율, 밀도, 비열 등 열물성 특성을 추가하여 GIS 공간 D/B구축하였다. 대륙붕 자료 4개 자료를 제외하고 359개의 지열류량 자료를 이용하여 한반도 남부, 즉 남한의 지열류량 분포도를 작성 분석해 본 결과(그림 1), 우리나라의 지열류량 이상대는 아산만 주변, 보령, 유성, 진안, 울진, 포항, 부산 지역과 포천, 속초, 충주, 수안보 등 지역에서 나타난다 이러한 이상대 주변에는 대개 온천이 발달되어 있었거나 새로 개발되어 있는 곳이다. 온천에 이용하고 있는 시추공의 자료는 배제하였으나 온천이응으로 직접적으로 영향을 받지 않은 시추공의 자료는 사용하였다 이러한 온천 주변 지역이라 하더라도 실제는 온천의 pumping 으로 인한 대류현상으로 주변 일대의 온도를 올려놓았기 때문에 비교적 높은 지열류량 값을 보인다. 한편 한반도 남동부 일대는 이번 추가된 자료에 의해 새로운 지열류량 분포 변화가 나타났다 강원 북부 오색온천지역 부근에서 높은 지열류량 분포를 보이며 또한 우리나라 대단층 중의 하나인 양산단층과 같은 방향으로 발달한 밀양단층, 모량단층, 동래단층 등 주변부로 NNE-SSW 방향의 지열류량 이상대가 발달한다. 이것으로 볼 때 지열류량은 지질구조와 무관하지 않음을 파악할 수 있다. 특히 이러한 단층대 주변은 지열수의 순환이 깊은 심도까지 가능하므로 이러한 대류현상으로 지표부근까지 높은 지온 전달이 되어 나타나는 것으로 판단된다.

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A Study on the Interactions between the Actors of the 3D Broadcasting Standardization Process (3DTV방송기술 표준화과정의 참여자간 상호작용 : 행위자 네트워크 이론기반 사례연구)

  • Song, Kyung Hee;Kwak, Kyu Tae;Park, Soo Kyung;Lee, Bong Gyou
    • Journal of Internet Computing and Services
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    • v.15 no.2
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    • pp.109-127
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    • 2014
  • This study is devised out of the recognition that the existing standardization-related research has not sufficiently examined the overall social environment where a standard is actually made and diffused and the roles of the actors and the changes in them in the complex social system where multiple stakeholders exist. Against this backdrop, this study purports to reconstruct the dynamic process of developing and standardizing an innovative technology through a socio-technical approach involved by multiple stakeholders with different interests in the context of a socio-technical institutional environment. The specific goals to achieve the purpose include first, inspecting the characteristics of the interactions between the human actors and between the human and non-human actors in the socio-technical network surrounding a standardization process. Second, the study aimed to observe the activities of the focal actor who led the standardization process and its changing role. To that end, it analyzed the dynamic features of the process of standardizing a HD 3DTV broadcasting technology that took place in South Korea based on the actor network theory. As for the analysis method, the researchers personally took part in the actor network involving the new technology to analyze the dynamic characteristics of the network, applying the qualitative research method of survey and in-depth interviews and exploring the overall dynamics of environment, behavior and technology observed over the course of the entire standardization process.

The Design and Implementation of A Video Geographic Information System (비디오 지리 정보 시스템의 설계 및 구현)

  • 유재준;주인학;남광우;이종훈
    • Proceedings of the Korean Information Science Society Conference
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    • 2002.10c
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    • pp.274-276
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    • 2002
  • 최근에 지리 정보를 검색, 관리, 분석할 수 있는 지리 정보 시스템(Geographic information System)의 사용이 확대됨에 따라 단순한 수치지도 정보 이외에 좀 더 현실적이고. 사실적인 정보를 제공해 줄 수 있는 시스템에 대한 요구가 커지고 있다 이러한 요구에 부응하는 한 방향으로써 차량에 부착된 카메라 등등 사용하여 획득한 비디오 데이터와 수치지도 정보를 연계하여 특정 수치지도 객체의 영상을 제공함으로써 수치지도 객체에 대한 자세한 현장의 정보를 쉽게 파악할 수 있도록 하는 비디오 지리 정보 시스템이 등장하였다. 하지만 기존의 비디오 지리 정보 시스템은 획득한 비디오 데이터를 수치지도의 한 속성으로서 표현하거나, 수치지도에서 비디오 데이터로의 단순한 링크를 사용하여 수치지도와 연계함으로써 수치지도의 검색을 통한 영상 정보의 출력이라는 단방향 검색만을 지원한다. 즉, 비디오 데이터에 존재하는 수치지도 객체의 검색을 통한 수치지도의 검색 및 관리, 분석은 지원해 주지 못하고 있다. 본 논문에서는 비디오 데이터와 수치지도의 양방향 검색 및 관리를 지원하는 비디오 지리 정보 시스템을 설계하고 구현한다. 이를 위해 1) 비디오 데이터와 수치지도 정보를 효율적으로 연계하기 위한 데이터 모델을 제안하고, 2) 비디오 데이터와 수치지도 정보의 연계 정보를 추출 및 구축하는 과정을 제안하며, 3) 비디오 데이터의 출력 및 검색, 수치지도의 출력 및 검색, 연계 정보를 통한 비디오 데이터와 수치지도 정보의 연동 등을 효율적으로 지원하기 위한 시스템을 컴포넌트(Component) 구조를 사용하여 설계한다. 이해성 및 이를 기반으로 하는 교육용 애플리케이션 개발의 용이성의 증대를 기대할 수 있으며, 모델의 재사용성을 보장할 수 있다. 제안한다.수행하였다. 분석에서는 제품의 효율성뿐만 아니라 보안성을 중요하게 생각하였으며, 앞으로 보안 관련 소프트웨어 개발에 사용될 수 있는 도구들이 가이드 라인에 대한 정보를 제공한다.용할 수 있는지 세부 설계를 제시한다.다.으로서 hemicellulose구조가 polyuronic acid의 형태인 것으로 사료된다. 추출획분의 구성단당은 여러 곡물연구의 보고와 유사하게 glucose, arabinose, xylose 함량이 대체로 높게 나타났다. 점미가 수가용성분에서 goucose대비 용출함량이 고르게 나타나는 경향을 보였고 흑미는 알칼리가용분에서 glucose가 상당량(0.68%) 포함되고 있음을 보여주었고 arabinose(0.68%), xylose(0.05%)도 다른 종류에 비해서 다량 함유한 것으로 나타났다. 흑미는 총식이섬유 함량이 높고 pectic substances, hemicellulose, uronic acid 함량이 높아서 콜레스테롤 저하 등의 효과가 기대되며 고섬유식품으로서 조리 특성 연구가 필요한 것으로 사료된다.리하였다. 얻어진 소견(所見)은 다음과 같았다. 1. 모년령(母年齡), 임신회수(姙娠回數), 임신기간(姙娠其間), 출산시체중등(出産時體重等)의 제요인(諸要因)은 주산기사망(周産基死亡)에 대(對)하여 통계적(統計的)으로 유의(有意)한 영향을 미치고 있어 $25{\sim}29$세(歲)의 연령군에서, 2번째 임신과 2번째의 출산에서 그리고 만삭의

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Effect Cross-national Brand Personality on Preference and Revisiting in Fastfood Restaurant (한.중 대학생의 브랜드 개성이 선호도 및 재방문에 미치는 영향)

  • Lee, Sang-Mi
    • The Journal of the Korea Contents Association
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    • v.9 no.9
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    • pp.403-410
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    • 2009
  • This paper is to find out which factors affect preference and revisiting on brand personality according to cross-national college students in Korea and China, and to suggest distinguished strategies for attracting core customers continuously on each country and restaurants. The questionary hand out each 150, then used for data analysis 142 in Korean, 122 in China. The results come up with; First, It couldn't compared huge gap for McDonald and Burger King in sampling. Second, three variables (successful, harmony, and western) are highly recognized brand personality in Korean. They should build up powerful image to communicate 20's ages. For Chinese that ranked highly three variables (Darling, Friendly, and Sincere), they reinforced warm-hearted image with special character, providing promotion coupon, and various event. Third, because 2 factors(Sincere and Sensitivity) had significant in preference for Korean and Chinese. It should be formed non-producted parts which are visual decoration, atmosphere, uniform and music. Also, 'feminine' had meaningful for Chinese. Fourth, 'Sincere' was connected directly in revisiting for Korean and Chinese. Trust Is prior to customer and brand. Also, for Korean, 'sensitivity' had significant. Therefore, it should make exciting surrounding, and atmosphere.