• Title/Summary/Keyword: 비색

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Colorimetric Method of Determining Unsaturated Fatty Acids in Animal Tissues (불포화지방산(不飽和脂肪酸)의 비색정량법(比色定量法))

  • Huh,, Rhin-Sou;Chang, In-Ho
    • Journal of Nutrition and Health
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    • v.15 no.1
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    • pp.9-14
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    • 1982
  • The present paper describes a colorimetric method of determining the free and total unsaturated fatty acids in animal tissues. The procedure is based in three steps on the following principles : First step is that the tissue homogenates are extracted in chilled acetone in order to eliminate the interfering substance, such as phospholipids, sulfatides and proteins. At next step, after centrifugation acetone layer is decanted and evaporated to dryness. Then the extract is shaken with heptane to solve in the solvent. That the characteristic nature of copper salts of unsaturated fatty acids are freely soluble in heptane and those of saturated acids are not is the bases of separating one from another. Thus unsaturated fatty acids can be isolated in heptane as their salts from saturated acids and other lipid mixture by shaking with copper reagent. Finally the yellowish brown color developed by adding color reagent (0.2% sodium diethyldithiocarbamate in n-butanol solution) which reacts with the copper salts of the acids and produces the color is measured colorimetrically.

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Studies on the Colorimetric Determination of Panaxadiol and Panaxatriol (Panaxadiol 및 Panaxatriol의 비색정량법에 관한 연구)

  • 남성희;유병무;김해중;이석건
    • Journal of Ginseng Research
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    • v.3 no.2
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    • pp.127-133
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    • 1979
  • A simple and rapid colorimetric method for determination of panaxadiol and , panaxadiol was developed. 1. After heating with 60% perchloric acid, panaxadiol and panaxadiol yielded red.purple color with absorption maximum at 540 nm and 538 nm, respectively. 2. The maximum colors of the Panaxadiol and panaxadiol were reached when the algycones were treated at 6$0^{\circ}C$, 5 minutes or 7$0^{\circ}C$ 3 minutes. 3. The absorbance varied linearly with the amount of aglycone in the reaction mixture. And the colorimetric method was sensitive to about 10$\mu\textrm{g}$ of aglycone in 5.5ml of the reaction mixture. 4. The color was stable for about a week at 4$^{\circ}C$. 5. $\beta$-Sitosterol, oleanolic acid and cholesterol were not yielded red color by treatment with 60% perchloric acid under the conditions described.

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Colorimetric Determination of Acidic Polysaccharide from Panax ginseng, its Extraction Condition and Stability (인삼 산성다당체의 비색정량 방법과 그 추출조건 및 안정성)

  • 도재호;이형옥
    • Journal of Ginseng Research
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    • v.17 no.2
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    • pp.139-144
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    • 1993
  • The method for colorimetric determination of acidic polysaccharide from Panax ginseng was investigated. It is possible to apply the method of carbazole-sulfuric acid to determination of pectin, and also to measure the amount of pectin in the mixture of various high molemlu compounds such as starch. cellulose and gum, etc. When the method of carbazole-sulfuric acid was applied to determine the amount of acidic polysaccharide, optical density at 525 nm increased linearly with an increase in the concentration of pure acidic polysaccharide. Effective extraction temperature with water for the determination of the amount of ginseng acidic polysaccharide (GAP) was $80{\circ}C$. In order to separate or concentrate GAP it was appropriate to precipitate the extract only once with 80% ethyl alcohol. GAP was very stable at $100{\circ}C$ for 4 hrs in aqueous solution and between pH values of 5.0~ 12.0.

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Application of Colorimetric Method for Evaluation of Apparent Chloride Diffusion Coefficient of Concrete (콘크리트 중 겉보기 염소이온 확산계수 추정을 위한 비색법의 적용)

  • 문한영;김홍삼;최두선;오세민
    • Proceedings of the Korea Concrete Institute Conference
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    • 2003.11a
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    • pp.541-544
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    • 2003
  • Chloride diffusivity is one of the important properties of concrete affecting the durability of a structure. The diffusivity for porous materials is determined conventionally by immersion in a solution. However, this method is complicate and time-consuming, often requiring months or years to obtain results. Thus, the application of colorimetric method to estimate the apparent diffusivity of chloride ion was verified in this study. The result reveals that the apparent diffusivity of chloride ion can be predicted to use colorimetric method. Additionally the colorimetric method is capable to predict the profile of chloride ion.

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A Study on the Calcium and Iron Content of the Undaria pinnatifida suringar (미역중의 Calcium 및 Iron 의 함량에 대하여)

  • Rhee, Sook-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.1 no.1
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    • pp.25-31
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    • 1972
  • The Undaria pinnatifida collected in the waters of Dongbaek Island were grouped into young and adult ones and calcium and iron contents of each were analyzed by parts, i.e., separately for blade, stripe, and sporophyll by potassium permanganate titration method and spectrophotometric method respectively (O-phenanthrolin used). The results showed that the blades contain higher percentage of calcium than other parts both in young and adult seaweeds and that proportionately twice as much iron is contained in the blades than in the stripes.

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The Analysis on the Oil Deterioration of Pole-Transformer by Non-Destructive and UV/Visible Method (비파괴 및 비색법에 의한 주상변압기 절연유의 경년열화 분석)

  • 곽회로;남영우;윤영자;권혁일;이동준;송일근;권동진
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 1995.10a
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    • pp.115-117
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    • 1995
  • This paper describes the O.T aging analysis by non-destructive electrical and UV/visible method. Non-destructive diagnosis method is tan8 test. Also the new method of the aging analysis, UV/visible method was introduced in this paper and compared with the non-destructive electrical method. The UV/visible test could be useful method for the O.T aging electrical diagnosis such as non-destructive electrical method.

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Application of Lignin (I). Preparation of Active Carbon and It's Adsorptivity (리그닌의 응용 (제1보). 활성탄 제조 및 성능조사)

  • Byung Kak Park;Byung Guen Lee;Gab Yong Lee
    • Journal of the Korean Chemical Society
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    • v.20 no.2
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    • pp.153-157
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    • 1976
  • The purpose of this paper is to use lignin. Lignin was obtained by digestion of Korean pine wood, purified, and identified. After carbonizing lignin by con $c-H_2SO_4$, the resulting carbon was converted into active carbon by activating it in the electric kiln. The adsorptivity of this active carbon was obtained by measurement with a spectro-photometer. It has been found as a result that adsorption power is 89%.

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A Clinical Observation of the Nasal Acupunture Therapy on Nasal Obstruction (비침이 비색증에 대한 영향)

  • Kim, Yoon-bum;Yoon, Sang-hyub
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.12 no.2
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    • pp.205-211
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    • 1999
  • We studied 32 patents, who had visited Kyung Hee downtown oriental hospital with nasal obstruction. They has been observed their nasal cavity through anterior rhinoscopy. We had paten't middle and inferior turbinte bleeding by acupuncture. This symptom had been disappeared after this treatment. The result were as follows. 1. Sex and age distribution: The males were 18($56.2\%) and Female were 14($43.8\%$). The most common occurrence was found between 1-5 years old. 2. The most common duration of disease was between 2-6 months. 3. In decreasing order, the common complication & past history of otolaryngologic or allergic disease were allergic rhinitis, atopic dermatitis, chronic sinusitis, adenoid or tonsil hypertrophy, otitis media with effusion and asthma. 4. In descending order, distribution of symptoms were rhinorrhea, sneezing, dry sensation, sputum, frontal headach, postnasal drip and cough. 5. The improvement rate in symptom were $31.3\%\;excellent\;and\;53.1\%$ good.

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Amino Acid Content of Korean Foods (1) (한국식품중 아미노산의 함량구조보고 (제1보))

  • 채례석;유정렬;안옥경;김문자
    • YAKHAK HOEJI
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    • v.5 no.1
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    • pp.27-30
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    • 1960
  • 단백질의 생물가는 그것을 구성하고 있는 각 아미노산의 양적및 질적조성에 기인하며 그들 아미노산의 양적 대소및 균형이 그 단백질의 영양가를 좌우하고 있다. 또한 인체및 동물에 대한 아미노산의 요구량까지도 보고되어 있다. 이와같이 단백질의 영양학적가치에 대한 개념이 아미노산에 대한 것으로 전환됨에 따라 식품중 아미노산의 함량조사가 여러 나라에서 보고되고 있다. 그러나 우리나라에서는 두류발효제품및 엽채단백질중의 유리 아미노산을 paperchromatography법에 의하여 검출한 것과 비색방법에 의한 양적변화 정도만이 보고되었을 뿐이다. 저자들은 미생물학적방법에 의하여 한국식품중 아미노산의 함량 조사를 착수하여 위선 지금까지 얻은 성적을 보고하고자 한다.

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Comparison of visual colorimetric Analysis and neural network algorithm in urine strip classification (뇨 스트립 분류에서 육안비색법과 신경회로망 알고리즘 비교)

  • Eum, Sang-hee
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.24 no.10
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    • pp.1394-1397
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    • 2020
  • The urine test used as a basic test method of in vitro diagnosis for health care has been used for a long time to be simple and convenient. The urine test method is using a color that appears depending on the change in the ion concentration that reacts over time buried in the standard color test paper(Strips) with a urine sample applied to some reaction reagents. In this paper, it was proposed a neural network algorithm to obtain a suitable and reproducibility and accuracy classifier suitable for the urine analysis system. The experimental results were compared with the visual colorimetric analysis, and the neural network algorithm showed better results.