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The Anti-obesity Effect of Aureobasidium pullulans SM-2001 Extract (Polycan^®) on 3T3-L1 Preadipocytes and Adipocytes (3T3-L1세포에서 흑효모 SM-2001 추출물(Polycan^®)의 항비만 효과)

Kim, Young-Suk;Lim, Jong-Min;Ku, Bon-Hwa;Moon, Seung-Bae;Cho, Hyung-Rae;Lee, Seon-Min;Kwon, Jung-Hee
- Journal of Life Science
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- v.30 no.10
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- pp.835-843
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- 2020
Obesity, the world's leading metabolic disease, is a serious health problem in both industrialized and developing countries. Natural substances are of great interest in preventative medicine, especially in the field of metabolic syndromes-from insulin resistance to obesity and diabetes. In the present study, we investigated the effect of A. pullulans SM-2001 Extract (Polycan^®) on the adipocyte differentiation of 3T3-L1 preadipocytes and the anti-obesity effect of 3T3-L1 adipocytes. Although β-glucan has been found to have health benefits in the regulation of the immune system and blood cholesterol levels, its role in obesity has not been fully investigated. Polycan^® suppressed lipid accumulation and glycerol-3-phosphate dehydrogenase (GPDH) activity without affecting cell viability in 3T3-L1 preadipocytes and adipocytes. Polycan^® also inhibited cellular lipid accumulation through down-regulation of transcription factors, such as PPARγ and C/EBPα, and induced dose-dependent phosphorylation of AMP-activated protein kinase (AMPK)-a cellular energy sensor-while the total AMPK protein content remained unchanged. Taken together, this shows that the activation of AMPK by Polycan^® in adipocytes plays a critical role in Polycan^®-induced inhibition of adipocyte differentiation. Our results show that Polycan^® has an anti-obesity action in vitro, suggesting a potential novel preventative agent for obesity and other metabolic diseases.
https://doi.org/10.5352/JLS.2020.30.10.835 인용 PDF KSCI HTML