• The Korean Journal of Zoology
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• v.34 no.4
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• pp.479-490
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• 1991

포유류 배아의 착상기전을 규명할 목적의 일환으로 자궁내막조직의 탈락막 형성과 분화에 미치는 난소 스테로이드 호르몬의 영향과 인위적 자극등의 영향을 조사하였다. 가임신을 유도시킨 흰쥐와 정상임신군의 자궁내막조직을 착상부위와 비 착상부위로 분리하여 자궁내막조직의 분화에 지표가 되는 Alkaline phosphatase(ALPase)의 활성을 측정하였다. 가임신군에서 인위적 자극(trauma)을 가한 자궁이 자극을 받지않은(control) 자궁에 비해 Progesterone(P)만을, 또는 progesterone과 estradiol(E + P)을 동시 처리받은 실험군에서 ALPase 활성이 유의한 차이(p<0.01)로 높게 나타났다. 정상임신군에서는 착상시기인 임신 제6일군의 착상부위에서 I처리군과 P처리군의 ALPase활성이 비착상 부위에 비해 유의한 차이(P < 0.05)로 크게 나타나며, 특히 임신 제6일군에서는 착상, 비착상 부위의 P처리군의 활성이 Intact군보다 유의하게(P<0.05) 높아졌다. 정상임신 제 9일 Intact군의 ALPase활성은 임신 제3일, 6일의 Intact 군의 활성에 비하여 착상(3, 6일 :P<0.01), 비 착상부위 (3일 :P<0.05, 6일 :P<0.02)에서 다같이 유의한 차이로 높아졌다. 본 연구결과에서 배아가 분화, 발생해 감에따라 자궁내막조직 분화도 활발해지고, 착상부위 분화는 착상시기부터 현저해지며, 특히 P호르몬의 영향이 크다는 것과 배아 아닌 인위적 자극으로도 탈락막 형성 유도가 가능하다는 것을 확인할 수 있었다.

• Journal of Life Science
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• v.17 no.6 s.86
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• pp.822-824
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• 2007

The efficient system for plant regeneration from cotyledon and primary feat explants of lettuce was established. Plant regeneration efficiency was shown 91.3% from cotyledon and 85.9% from primary leaf explants of variety 'Jungtongpogi' in KN medium. Plant regeneration efficiency was also estimated with various plant regeneration media in variety' Chungchima', which was lowest plant regeneration efficient showing 35.4% from cotyledon and 30.3% from prima leaf explants in KN medium. Kl medium increased 77.9% and 80.7% of plant regeneration efficiencies from cotyledon and primary leaf explants of variety 'Jungtongpogi' were cultured on KN medium. In case of varie쇼 ‘Chungchima', efficient plant regeneration was shown when primary leaf explants were cultured on SH and KI media.

• Journal of the korean academy of Pediatric Dentistry
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• v.42 no.4
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• pp.312-318
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• 2015

The aim of this study was to investigate the possibility of the supernumerary teeth for the stem cell source in dentistry. The Real Time Quantitative Reverse Transcription Polymerase Chain Reaction (Real Time qRT-PCR) method was used to evaluate the differentiation toward the odontoblast of the supernumerary dental pulp stem cells (sDPSCs). Supernumerary dental pulp stem cells were obtained from 3 children (2 males and 1 female, age 7 to 9) diagnosed that the eruption of permanent teeth was disturbed by supernumerary teeth. The common genes for odontoblasts are alkaline phosphatase (ALP), osteocalcin (OC), osteonectin (ON), dentin matrix acidic phosphoprotein 1 (DMP-1), dentin sialophosphoprotein (DSPP). The sDPSCs were treated for 0 days, 8 days and 14 days with additives and then Real Time qRT-PCR was performed in intervals of 0 days, 8 days and 14 days. The alizarin-red solution staining was performed to visualize the stained color for the degree of calcification at 7 days, 14 days, 21 days and 28 days after treating additives to the sDPSCs. From the result of the Real Time qRT-PCR, the manifestation exhibit maximum value at 8 days after additive treatment and shifted to a decrease trend at 14 days. Alizarin-red solution staining exhibit light results at 7 days after staining and generalized dark result at 14 days. Consequently, in studies with sDPSCs, appropriate treatment time of additives for Real Time qRT-PCR is 8 days. Also, a suitable period of Alizarin-red solution staining is 14 days.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.2
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• pp.116-122
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• 1982

In three diverse wheat and one barley cultivars were used to examine the relation of the spikelet and floret differentiation to fertility and an winter wheat Norin 61 was used to examine the spikelet size gradients in the mature ear in relation to their grain development. The greatest number of grains and florets per spikelet, and the heaviest grains occurred in the low-mid part of the ear. Within the spikelets, the 2nd grain from the base was heavier than the I st or the 3rd one. In this region, the fertility of the 1 st floret was 97%, the 2nd 93%, the 3rd 88%, and 4th floret was 15%. The fertility of the wheat and barley was closely related to spikelet and floret differentiation. Barley spikelets was fertile as their differentiation stage was X that is 25 days before heading. Wheat florets were fertile as their differentiation stage was X that is 15-20 days before heading. But, they were sterile or reduced in fertility if they were not reached to the stage X.

• The Korean Journal of Zoology
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• v.38 no.1
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• pp.87-95
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• 1995

한국산 담수어류의 잉어목, 황어아과(Leuciscinae) 어류 2속 5종의 계통적 유연관계를 구명하기 위하여 mtDNA분석을 실시하였다 6 base를 인지하는 10개의 제한효소를 처리하여 얻어진 강tDNA의 크기는 16.5-17.5 Kb였으며 Bcl I, Bgl I, Bgl II, Hin dIII, Pvu II, Xba I 등은 종간 차이가 뚜렷하였다. 각종의 집단간 mtDNA분화정도는 매우 낮았으나(p=1% 미만) M. oxyephalus의 무주집단과 제주집단은 예리적으로 큰 차이를 보였다(p=5.3%) Moroco속의 종간 분화정도를 비교한 결과 M. oxycephafus와 M. lagowsk서 사이가 평균 f=7.2%로 근면관계가 제일 가까웠고 M. keumkang과 M. semotilus는 타종들과 근연관계가 제일 멀었다. Moroco속과 Phoxinus속간의 평균 유전적 분화정도는 f=13.7%로 현저한 차이를 보였다. Brown 등(1979)의 공식을 이용하여 이들 황어아과 2속 5종의 분화시기를 추정한 결과 이들은 후기 선신세(Pliocene)와 흥적세(Pleistocene) 사이에 분화된 것으로 추정되었으며 이 결과는 동위효소 연구에서 얻어진 결파(Yang and Min, 1989)와 잘 일치한다.

• Journal of Plant Biology
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• v.35 no.1
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• pp.17-23
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• 1992

Effects of polyamines and their biosynthetic inhibitors on adventitions shoot regeneration of Populus leaf segments were investigated. Polyamine inhibitors such as difluoromethyl arginine (DFMA), difluoromethyl ornithine (DFMO), and dicyclohexylamine (DCHA) decreased the fresh weight of cultured leaf segment and the rate of adventitious shoot regeneration. The inhibitory effects of DCHA were stronger than any other oplyamine inhibitors, and the rest were in the order of DFMO and DFMA. The inhibitory effects of these inhibitors were lessened or disappeared by the addition of polyamines, among which spermidine was the highest in its effect. Therefore, it is suggested that the spermidine may be related to the adventitious shoot regeneration of Populus leaf segments.gments.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.948-955
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• 2007

Neutrophils play a key role as a first line of defense and are known to acquire the characteristics of dendritic cells (DCs) under the appropriate conditions. The spontaneous apoptosis of neutrophils was delayed by treatment with 4-(2-aminoethyl) benzensulfonylfluoride (AEBSF), a serine protease inhibitor. AEBSF inhibited both caspase-3 and serine protease activities, whereas ZVAD-fmk, a pancaspase inhibitor, inhibited only caspase-3 activity. The life span of neutrophils was prolonged up to 5 days by AEBSF in the presence or absence of granulocyte macrophage colony stimulating factor(CM-CSF). DC surface markers, such as CD80, CD83, and MHC class ll were not expressed on neutrophils treated with AEBSF alone. CM-CSF failed to prolong the survival time of neutrophils up to3 days but increased the expression levels of DC markers on neutrophils in the presence of AEBSF. Expression levels of DC markers were the highest on neutrophils treated with CM-CSF and AEBSF for 3 days. AEBSF and CM-CSF-treated neutrophils stimulated proliferation of T cells in the presence of a superantigen, Staphylococcal enterotoxin B (SEB) but produced $interferon-{\gamma}$ ($IFN{\gamma}$) in the absence of SEB. These results suggest that the inhibition of serine protease activity prolonged the life span of human neutrophils and combined treatment of neukophils with CM-CSF and serine protease inhibitor induced differentiation of neutrophils into DC-like cells.

• The Korean Journal of Zoology
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• v.35 no.4
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• pp.526-533
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• 1992

포유류 배아의 착상기작을 규명하고자 본 연구에서는 착상시기에 수정된 배아가 착상부위를 어떻게 인식하는 지 그리고 착상부위의 분화유발요인이 무엇인지를 조사하였다. 착상시기(임신 제6일)와 착상후 초기배아 발생기간(임신 제9일)중 혈청내 estradiol (E2)과 Progesterone(P4)의 농도를 측정하였으며, 자궁내막 조직을 착상부위 (antimesome trium)와 비 착상부위(mesometrium)로 분리하여 P4의 수용체 농도를 측정하였다. 혈청내 E2의 농도는 임신 제6일군에서 E2처리군에서 가장 높았으며, 임신 제9일에서는 intact 대조군에서 가장 높고 제9일의 모든 실험군의 농도는 임신 제6일군보다 높았다. 혈청내 P4 농도는 임신 제6일과 9일에서 다같이 대조군에서 가장 높으며 처리군 중에서는 P4처리군에서 높아지고 있다. P4 농도 역시 임신 제9일의 모든 실험군에서 제6일의 실험군보다 높았다. P4수용체 농도는 착상부위가 비 착상부위보다 높으며, 대조군(P < 0.01)과 P처리 군(P < 0.05)에서는 유의한 차이로 비 착상부위보다 높았다. 자궁내막조직 착상을 위한 분화에는 P4의 영향이 크며 P4의 혈청내 농도와 핵의 수용체 농도는 모든 실험군에서 상응하는 관계를 나타내었다. 이 결과는 앞서 일차적으로 발표한 알카리성 phosphatase (ALPase) 활성과도 상응하는 것이다.

• The Korean Journal of Zoology
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• v.30 no.4
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• pp.401-409
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• 1987

단백질 분해효소와 ovoinhibitor가 계배근세포 융합에 미치는 영향을 알아보기 위하여 세포 운합지수의 측정, Western blot 분석법에 의한 ovoinhibitor의 세포 내농도 측정 및 단백질 분해효소의 활성도를 조사하였다. 세포 배양 30시간과 72시간에 처리된 ovoinhibitor는 상당히 근세포 융합 억제시켰다. 근세포 내 ovoinhibitor의 농도는 배 양 48시간에서 가장 높았고, 배양이 진행됨에 따라 계속 떨어져서 대체로 융합지수가 올라갈수록 그 농도가 낮아지는 경향을 보였다. 근세포 분화가 진행됨에 따라 총 단백질 분해효소의 환성도는 계속 증가하였는데 특히 배양 48시간에서 72시간 사이의 급격한 증가를 나타내는 시기는 세포 융합이 가장 활발히 일어나는 시기와 일치했다. 이상의 결과는 단백질 분해효소 저해제가 근세포 분화에 상당한 영향을 끼침을 보여 주며, 단백질 분해효소와 단백질 분해효소 저해제는 하나의 조절단위로써 근세포 분화에 어떤 작용을 할 것으로 사료된다.

• Applied Microscopy
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• v.31 no.2
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• pp.143-156
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• 2001

The aim of this study was to clarify the differentiation of seminiferous epithelial cells and spermiogenesis in the testis of Rana catesbeiana. Spermatogenesis of R. catesbeiana consists of primary spermatogonia, secondary spermatogonia, primary spermatocytes, secondary spermatocytes and spermatids. They were subdivided into eight stages on the basis of the morphological features of the germ cell differentiation. From the spermatocytes except primary spermatogonia to before the spermiation of spermatids were surrounded by spermatocyst. Spermiogenesis of R. catesbeiana can also be divided into three stages on the basis of morphological features of the nucleus and the cytoplasm organelles. Spermatozoon contained a saccular acrosome, a cylindrical and tapered slighty at both ends head, and a tail with only the axoneme.