• Korean journal of applied entomology
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• v.47 no.1
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• pp.37-44
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• 2008

Oriental fruit moth, Grapholita molesta, is a serious pest on apples. To control this pest in an environmentally friendly method, mating disruption strategy using sex pheromone has been developed. Area-wide application of mating disruption has been needed to be effective, with little understanding on how much size of apple cultivating area should be treated in one time application of the mating disruption technique. On this matter, we needed to determine a minimal mating active zone of G. molesta that should be applied with mating disrupters to be effective. Molecular markers to discriminate a specific population should be developed to trace population migration for reproductive behaviors. Here we developed two effective molecular markers using random amplified polymorphic DNA (RAPD) technique. Different field populations of G. molesta, based on locations and seasons, were analyzed with these markers. In a specific location, G. molesta populations varied in genetic composition with different seasons. Different local populations showed differential variation according to their relative distances among apple orchards. In overall, genetic variation among different populations became lessen with progression of seasons.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2001.11a
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• pp.66-73
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• 2001

• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.90-90
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• 2011

배기속도 2,500 L/s, 최고진공도 10-10 mbar를 구현할 대용량 복합 분자펌프 설계를 위한 2차원과 3차원 형상을 대상으로 실시하였다. 진공도가 10-5 mbar 이상이 되는 고진공도에서는 Knudsen 수가 102~107에 이르러 분자간 충돌을 거의 무시할 수 있게 되며, 이때의 유체해석 방법으로서는 통상 희박기체 해석법으로 많이 쓰이는 Direct Simulation Monte Carlo (DSMC) 방법보다, 충돌이 없는 분자의 자유운동을 모사하는 Monte Carlo 방법이 더 적합하게 된다. 본 연구에서는 다단계 rotor와 stator로 구성되는 복합분자 내 유동장에 Monte Carlo 해석법과 DSMC 방법을 모두 적용하여 유동해석을 실시하였다. 먼저 2차원 모델에 대한 해석을 실시하여 분자펌프의 성능에 중요한 영향을 미치는 설계변수로 날개각과 날개간격이 현저함을 확인하였으며, 이 설계변수들이 펌프의 주요성능 지표인 최대펌핑효율과 최대압축비에 미치는 영향을 다양한 3차원 유동해석을 통해 도출하였다. 유동해석 결과, 기체분자와 rotor 날개사이의 충돌 확률을 높이는 방안이 대체적으로 펌프의 성능을 향상시키는데 도움이 되는 것으로 나타났다.

• Journal of the Korean Society for Marine Environment & Energy
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• v.15 no.3
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• pp.247-256
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• 2012

Stable carbon isotope ratio of oil components are known to be unaffected by weathering processes and thus has been widely used to determine the origin of spilled oil. In this study, molecular index and composition of stable carbon isotope in 15 crude oils and petroleum product were analyzed and used as oil fingerprints to determine the discriminating power of each fingerprinting method among target crude oils. Through the fingerprints of alkane distribution only Bintulu and B-C(1%) were distinguishable from other crude oils. The pristane/phytane ratio can classify the crude oils into three groups but differentiation of crude oils within a group was impossible using the ratio. The crude oils of A.L., A.S.L., Foroozan and B-C(1%) were differentiated from the other oils using PAH source recognition indexes of C2D/C2P and C3D/C3P. The usage of 4-mD/1-mD and 2/3-mD/1-mD ratio was able to distinguish A.S.L., Bintulu and Oman from the other crude oils. However the PAH source recognition ratios in the other crude oils were similar and thus they were impractical to be used for source identification among the target crude oils. Stable carbon isotope ratios of alkanes were able to uniquely specify each crude oil in the plot of ${\delta}^{13}C_{C21}$ and ${\delta}^{13}C_{C25}$ except A.L., A.M., Qatar-Marine, B-C(1%). The oil fingerprinting method using stable carbon isotope ratios of individual alkane compounds showed more discriminating power among the target crude oils than the conventional source recognition indexes of PAHs or alkanes.

• Journal of Environmental Impact Assessment
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• v.26 no.3
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• pp.207-216
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• 2017

Several bioassays have been performed for assessment of the impact of polluted sediments. The direct exposure method using sediments is limited by difficulty controlling feeding and its effects on organisms. Furthermore, only macro-organisms and benthic organisms are used. To evaluate the potential application of sediment elutriate as a complementary strategy for impact assessment, copepods, small organisms with a short life cycle, were exposed to sediment elutriates, and several end-points were measured. As a result, sediment elutriates prepared from polluted sites caused growth retardation in marine copepods. In terms of molecular biomarkers, antioxidant-related and chaperone protein gene expression levels were increased in a dose- and time-dependent manner. Thus, we suggest that sediment elutriate tests can provide an effective alternative for toxicity assessment using whole sediment samples. Further studies are required to obtain sufficient data for future applications.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2001.11a
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• pp.58-65
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• 2001

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2004.05a
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• pp.69-69
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• 2004

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2002.05a
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• pp.137-160
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• 2002

• Korean Journal of Ecology and Environment
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• v.53 no.2
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• pp.165-172
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• 2020

Heavy metals are common pollutants in the freshwater environment and have toxicological effect in habitat organisms. The heavy metals highly accumulated in sediment and organism, and observed various physiological responses. In this study, we investigated the molecular response to heavy metal toxicity (Al, Aluminum; Cr, Chromium; Cu, copper; Mn, Manganese; Zn, Zinc) through expression of heat shock protein 40, 70, 90 (HSP40, 70, 90), cytochrome 450 (CYP450), Glutathione S-transferase (GST) and Serine-type endopeptidase (SP). HSPs showed up-regulation in Cu and Zn exposures. Furthermore, HSPs expression in treated groups tended to be higher than the control group. The tendency of CYP450 and GST mRNA expression was higher for Cr and Cu than for other exposure group. The expression of SP gene was low at Al exposure and other group were measured to be similar to control. These results suggest that heavy metal toxicity in freshwater ecosystem may affect physiological and molecular process. Also, the comprehensive gene expression in the aquatic midge Chironomus riparius give useful information to potential molecular biomarkers for assessing heavy metal toxicity.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.08a
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• pp.204-204
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• 2012

고용량/대면적/초정밀 제품을 지향하는 개발추세에 따라 고진공 펌프중 하나인 크라이오펌프의 활용도가 크게 증가하며 첨단 공정장비인 LCD 분야 등과 같이 수소 분자와 물 분자 등의 배기가 중요한 응용분야에 크라이오펌프의 수요가 대부분을 차지하고 있다. 그러나 크라이오펌프의 운전성능에 대한 표준절차에 따른 실질적인 측정은 빈약한 편이다. 이에 크라이오펌프의 종합 성능평가 기술 개발을 통해 실질적인 크라이오펌프의 운전성능을 제시하고자 한다. 본 연구에서는 고진공 펌프중의 하나인 크라이오펌프의 종합 성능평가 표준절차 및 표준시스템에 대해 소개하며, 상용화 제품에 대한 각 회사에서 제시하고 있는 성능지표에 대해 실질적인 측정에 대한 비교분석을 하고자 한다. 또한 가장 중용한 성능지표 중의 하나인 배기속도 측정에 대해 물리적 특징과 구조적 오차에 대한 보정 식들을 제공하고자 한다.