• Applied Microscopy
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• v.39 no.4
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• pp.333-340
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• 2009

In the present study, ultrastructural features of the mesophyll tissue have been investigated in Crassulacean acid metabolism (CAM)-performing succulent Orostachys. A large central vacuole and numerous small vacuoles in the peripheral cytoplasm were characterized at the subcellular level in both developing and mature mesophyll cells. The most notable feature was the invagination of vacuolar membranes into the secondary vacuoles or multivesicular bodies. In many cases, tens of single, membrane-bound secondary vacuoles of various sizes were found to be formed within the central vacuole. multivesicular bodies containing numerous small vesicles were also distributed in the cytoplasm but were better developed within the central vacuole. Occasionally, electron-dense prevacuolar compartments, directly attached to structures appearing to be small vacuoles, were also detected in the cytoplasm. One or more huge central vacuoles were frequently observed in cells undergoing differentiation and maturation. Consistent with the known occurrence of morphologically distinct vacuoles within different tissues, two types of vacuoles, one representing lytic vacuoles and the other, most likely protein storage vacuoles, were noted frequently within Orostachys mesophyll. The two types coexisted in mature vegetative cells but did not merge during the study. Nevertheless, the coexistence of two distinct vacuole types in maturing cells implies the presence of more than one mechanism for vacuolar solute sorting in these species. The vacuolar membrane is known to be unique among the intracellular compartments for having different channels and/or pumps to maintain its function. In CAM plants, the vacuole is a very important organelle that regulates malic acid diurnal fluctuation to a large extent. The membrane invagination seen in Orostachys mesophyll likely plays a significant role in survival under the physiological drought conditions in which these Orostachys occur; by increasing to such a large vacuolar volume, the mesophyll cells are able to retain enormous amounts of acid when needed. Furthermore, the mesophyll cells are able to attain their large sizes with less energy expenditure in order to regulate the large degree of diurnal fluctuation of organic acid that occurs within the vacuoles of Orostachys.

• Journal of Plant Biotechnology
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• v.35 no.3
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• pp.177-183
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• 2008

Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless(Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626(NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2(C3-H2-C3 type) domain for this group of protein is $Cys-X_2-Cys-X_{28}-Cys-X-His-X_2-His-X_2-Cys-X_{14}-Cys-X_2-Cys$. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.

• Journal of KIISE:Computer Systems and Theory
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• v.34 no.11
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• pp.545-561
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• 2007

Location management and message delivery protocol is fundamental to the further development of mobile agent systems in a multi-region mobile agent computing environment in order to control mobile agents and guarantee message delivery between them. However, previous works have some problems when they are applied to a multi-region mobile agent computing environment. First, the cost of location management and message delivery is increased relatively. Second, a tracking problem arises. finally, cloned mobile agents and parent-child mobile agents do not get dealt with respect to location management and message delivery. In this paper, we present a HB (Home-Blackboard) protocol, which is a new location management and message delivery protocol in a multi-region mobile agent computing environment. The HB protocol places a region server in each region and manages the location of mobile agents by using intra-region migration and inter-region migration. It also places a blackboard in each region server and delivers messages to mobile agents when a region server receives location update form them. The HB protocol can decrease the cost of location update and message passing and solve the tracking problem with low communication cost. Also, this protocol deals with the location management and message passing of cloned mobile agents and parent-child mobile agents, so that it can guarantee message delivery of these mobile agents and pass messages without passing duplicate messages.

• Journal of fish pathology
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• v.9 no.1
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• pp.21-31
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• 1996

Concerned to the Ichthyophthiriasis of aquacultural fishes, the developmental features of Ichthyophthirius multifiliis were studied in cultured Korean catfish, Silurus asotus, and rainbow trout, Oncorhynchus mykiss. In the morphological observation, the parasite developed on two kinds of parasitic and non-parasitic phases with 6 developmental stages termed phoront, trophont, protomont, tomont, tomite, and therone. The $60{\times}40-100{\times}70{\mu}m$ fusiform or spherical phoront for the invading stage has 34-38 meridional kineties and begins to develope buccal apparatus. The 80-$800{\mu}m$ spherical or amoeboid trophont for the vegetative stage has a horseshoe shape macronucleus, a inconspicuous cytostome and developmental contractile vacuoles. The 200-$800{\mu}m$ spherical protomont for the encysting stage has a inconspicuous macronucleus, abundant contractile vacuoles; and a fine gelatinous exocyst is exuded, the baccal apparatus begins to resorb. The tomont for the encysted dividing stage has a thick cyst wall, and the buccal apparatus is resorbed completely. A small 35-$50{\mu}m$ spherical tomite for each daughter cell has a cytostome end the conspicuous oral apparatus. The $25{\times}20-60{\times}40{\mu}m$ fusiform theront for the infective stage possesses a perforatorium in the anterior end, a cytostome in the mid-point respectively and has 34-38 meridional kineties. In the experiments of the reproductive, the excysted time is related to water temperature. Tomitogenesis takes 10-14 hours at $28^{\circ}C$, 12-15 hours at $26^{\circ}C$, 16-18 hours at $22^{\circ}C$, 24-28 hour at $18^{\circ}C$, 26-51 hours at $14^{\circ}C$, and 129 hours at $9^{\circ}C$ respectively.

• Sleep Medicine and Psychophysiology
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• v.19 no.1
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• pp.42-46
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• 2012

Introduction: The objective of the present study was to investigate the defense style of insomnia patients and to grasp the differences in defense style between primary insomnia patients and insomnia patients with history of major depressive disorder. Methods: Forty three subjects with insomnia (11 subjects with primary insomnia and 32 subjects with major depressive disorder) and 138 control subjects participated in this study. To diagnose insomnia and major depressive disorder, interviews including structured clinical interview for DSM-IV (SCID-IV) were done. To assess the defense style, self-reported Korean version of Defense Style Questionnaire (K-DSQ) were completed by the participants. Results: Compared to normal controls, subjects with insomnia used more acting out (t=3.25, p<0.01), consumption (t=2.66, p<0.01), fantasy (t=3.51, p<0.001), resignation (t=5.42, p<0.001), suppression (t=3.28, p<0.01), projection (t=3.92, p<0.01), splitting (t=4.31, p<0.01), undoing (t=2.66, p<0.01), withdrawal (t=6.72, p<0.001) and isolation (t=3.80, p<0.001), and less omnipotence (t=4.08, p<0.001) and humor (t=3.20, p<0.01). Compared to normal controls, subjects with primary insomnia used more undoing and withdrawal. Compared to subjects with primary insomnia, subjects with insomnia with history of major depressive disorder used more resignation and withdrawal, and less humor. Conclusion: In the current study, there were differences in defenses between primary insomnia patients and insomnia patients with major depressive disorder history. To evaluate the pattern of defenses through the K-DSQ might provide important clues to differentiate these two conditions.

• Journal of Bio-Environment Control
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• v.23 no.2
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• pp.77-82
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• 2014

The present study was conducted to establish an effect and a proper concentration for treatment with gibberellic acid ($GA_3$) and thidiazuron (TDZ), resulting with increase berry size and yield in Gaeryangmeoru grapes. Berry size was increased by treatment with $GA_3$, and the fruit clusters obtained for the groups treated with $GA_3$ concentrations of 100 and $200mg{\cdot}L^{-1}$ were bigger. The berry number was also enhanced in $GA_3$ treated groups, but the soluble solid content and acidity was not significantly different. Damage caused by $GA_3$ treatment, such as peel pollination and berry shatter, was observed in the group with $200mg{\cdot}L^{-1}$. The berry size was larger in group treated with a high concentration of $GA_3$ and TDZ respectively than in those treated with low concentrations in the treatment mixed $GA_3$ and TDZ; however, fruit with low soluble solid content and high acidity was harvested after $GA_3$ and TDZ treatment due to delay of berry ripening. The pericarp tissue layers were not changed, but the distance from the epidermis layer to vascular bundle tissue was increased as a result of $GA_3$ and TDZ treatment. Therefore, $GA_3$ and TDZ did not affect an cell division but not cell size, resulting in an enlarged berry size. It is necessary to treat plant growth regulators 2~3 times and immediately after berry set to enhance berry set rate, because the period of berry set is short. This study suggests that the proper concentration for enhancing berry size and set were up to $100mg{\cdot}L^1$ $GA_3$ or $50mg{\cdot}L^{-1}GA_3+1.25mg{\cdot}L^{-1}$ TDZ, and it is necessary to pay attention to harvest mature fruits because of the delay of ripening caused by the usage of TDZ.

• Clinical and Experimental Reproductive Medicine
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• v.36 no.1
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• pp.63-70
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• 2009

Objective: This study was performed to investigate the effectiveness of minimal stimulation using rhFSH and GnRH antagonist compared with GnRH antagonist multidose protocol (MDP) in IVF treated patients with aged 40 and above. Methods: Seventy-five patients with aged 40 and above were equally randomized to minimal stimulation group (n=37) or GnRH antagonist MDP group (n=38). For minimal stimulation group, ultrasound monitoring was started on cycle day 7 or 8. Daily injections of 0.25 mg cetrorelix together with 150 IU rhFSH were started from the day at 13${\sim}$14 mm of a leading follicle diameter. For GnRH antagonist MDP group, daily injections of 225 IU rhFSH were initiated from cycle day 2 and GnRH antagonist was started at a dose of 0.25 mg/day on rhFSH stimulation day 6 or the day at 13${\sim}$14 mm of leading follicle diameter. In both groups, transvaginal ultrasound-guided oocyte retrieval was performed. According to cleavage and morphologic characteristics of embryos, embryos were transferred 3 to 5 days after oocyte retrieval. Results: There were no differences in patients' characteristics and cycle cancellation rate between the two groups. Total dose and duration of rhFSH used were significantly fewer and shorter in minimal stimulation group than those in GnRH antagonist MDP group. The numbers of oocytes retrieved, mature oocytes and transferred embryos were also lower in minimal stimulation group. However, there were no significant differences in the clinical pregnancy rate and miscarriage rate between the two groups. Conclusions: This study demonstrates that minimal stimulation protocol provides comparable pregnancy rates to GnRH antagonist MDP with fewer dose and days of rhFSH used, and thus can be a cost-effective alternative in women aged 40 and above.

• Sijohaknonchong
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• v.22
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• pp.27-55
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• 2005

Visual media are taken the highest position in modem society, Modern poems also have been changed into visual poems, This aspect is the result of considering only individual talents ignoring traditions. Now, new Sigo should be concentrated on the mythological and historical voice from true nature and the body of human being, That is. ut should be converted into an ecological world view resolutely and restored a form of expression granted specific characteristics of our language. Advantages the computer media have brought. that is. equality freedom. human rights. harmony. pro-environmental value. can be maximized by positively accepting an ecological world view of Sijo which had included daily lives and spirits of the nation. Moreover. these all changes of new Sijo have to be established and recreated in the traditional expressions of Sijo. Aesthetic value of Sijo should be found in the expression forms such as phonetic harmony, rules of versification, rhythm, and etc. Then, we can overcome modern society's pathological phenomena such as severance, separation, dissolution, estrangement, psychiatric syndrome and etc. which visual media superiority brought. At the same time. it will cure ills of modern poems, Sijo and writing epochally and can establish true happiness and development.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.407-414
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• 2013

This study was carried out to understand the physiological characteristics of 'Manpungbae' (Pyrus pyrifolia Nakai) pears through the seasonal changes of pericarp structure and anatomical differences between bagging and non-bagging treatment, and also fruit quality and peel coloration characteristics at the harvest time. The pericarp at full bloom was consists of outer epidermis, hypodermis, parenchyma cell, and inner epidermis from the exterior. The cell layers from the outer epidermis to vascular bundle increased rapidly 7-10 layers to 18-26 layers from full bloom (FB) to 77 days after full bloom (DAFB) and did not change significantly until maturity. Thus, the cell division period of 'Manpungbae' pear was until 77 DAFB and during this period, the thickness from hypodermis to vascular bundle increased from $73.1{\mu}m$ to $195{\mu}m$ in this period. Stone cells were formed from seven to 21 DAFB and stone cell clusters were formed around 49 DAFB. The cork cell layer was formed between 49 and 77 DAFB. 'Manpungbae' fruit pericarp was consists of 4.5 layers of the cork cell layers and seven layers of hypodermis which has the tannin at harvest time (161 DAFB). Comparison of the fruit enlargement and fruit structure development by bagging or non-bagging showed that 'Manpungbae' fruits without bagging had more than three cork cell layer than those with bagging at maturity. The size of stone cell clusters were varied in two treatments. Fruit weight was higher in the non-bagging treatment but there was no difference in soluble solid contents (SSC) between two treatments. The weight of the 'Manpunbae' fruit was distributed from 301 g to more 900 g and the average fruit weight was 677.2 g at harvest time, and fruits in the range of 551-800 g accounted for 71.6% of total production. The SSC, acidity and SSC/acidity ratio was $10.2-12.1^{\circ}Brix$, 0.10-1.24% and 9.76-14.31 respectively, and the SSC was higher in bigger fruit which had a very higher positive correlation with a fruit weight. However, the fruit firmness tended to be lower with fruit size which had a very higher negative correlation with the fruit weight and SSC. The cork cell layer numbers between yellowish brown and green pericarp were not different significantly, in 3.8 and 3.5 respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.1
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• pp.8-15
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• 1992

An experiment was conducted to find out effect of soil water condition on shoot and root growth and P, K uptake by soybean plants. Soybean plants were grown under different soil water table levels, 20cm, 40cm and 60cm below the soil surface using minirhizotron with 20cm in diameter, and well irrigated and water stressed conditions using 1/2000a Waganer pots. Three soybean plants, Paldal culfivar, were grown and sampled at the early growing period, 37 days after planting, and at the harvesing period, 115 days after planting. Shoot and root growth were restricted by water stresed condition and by excessive soil water condition with the 20cm water table. Little difference in shoot and root growth were found between well irrigated condition and 40cm or 60cm water table conditions. The P and K contents in shoot under water stressed condition were higher than well irrigated condition at the early growing period but reversed at later harvesting period. The dry weight and length of roots were more severely restricted by water stress than those of shoots. Root morphological difference was found by anatomical observation. Normal cortex was developed under the well irrigated condition, while abnormal cortex with aerenchyma formed by lysis under excessive water conditions of 20 or 40cm water talbes. Aerenchyma was formed at outer skirt of pericycle. Role of formation of aerenchyma of soybean roots might be an adaptation to excessive soil water condition and possibly related to survival of soybean roots growing under near flooding condition on dikes of paddy lands.