• Research in Plant Disease
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• v.30 no.1
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• pp.26-33
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• 2024

In 2023, symptoms like damping-off disease were observed in 74 paprika growing in greenhouses in Cheorwon-gun, Gangwon-do, Korea. In this study, we tried to find the cause of the damping-off disease outbreak. We collected symptomatic seedlings and observed the typical crescent-shaped conidia of Fusarium oxysporum by microscope. To confirm the presence of F. oxysporum in the samples, polymerase chain reaction was performed using primers specific for F. oxysporum; the resulting sequence showed 99.11% identity with F. oxysporum. To confirm the pathogenicity of the F. oxysporum (CW) isolated from the samples, healthy paprika plants were inoculated with F. oxysporum CW and damping-off symptoms were observed 2 weeks later. To investigate whether the damping-off disease outbreak in Cheorwon-gun was caused by F. oxysporum-contaminated seeds, 100 paprika seeds were disinfected and placed in Murashige and Skoog medium. Typical pink F. oxysporum hyphae were found only in control non-disinfected seeds. An 18S rRNA-based and a TEF genebased phylogenetic analysis showed that the F. oxysporum CW isolate was not grouped with a F. oxysporum isolate reported from Cheorwon-gun in 2019. This study is the first report that an outbreak of damping-off disease in paprika in Cheorwon-gun, Gangwon-do, Korea, was caused by contamination of F. oxysporum seeds.

• Journal of Life Science
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• v.19 no.5
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• pp.561-565
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• 2009

Human Tissue factor is an essential enzyme activator that forms a catalytic complex with factor VII/ VIIa, and catalyzes both the extrinsic and intrinsic blood coagulation cascades. The extracellular domain of human tissue factor is responsible for association with the biological partner. The efficient procedures for preparing biologically active human tissue factor are essential for the preclinical and clinical studies with coaguligands. An expression vector in Escherichia coli has been constructed to direct the production of extracellular human tissue factor without a fusion protein or a $His_6$ at the N-terminus. The recombinant human tissue factor was expressed in large amounts as a non-native state in E. coli. The recombinant protein was simply renatured during the DEAE-sephacel chromatographic purification procedure. Our expression and purification system does not require a protease treatment or an additional chromatographic step to remove a fusion contaminant, which provides a very useful alternative to conventional expression systems for the production of human tissue factor.

• Journal of the Korean Electrochemical Society
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• v.14 no.4
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• pp.219-224
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• 2011

Using an electrolyte additive, we examined, for the first time, a novel self-ordering regime of 160~200 V in high-field anodization which had been used for a fast fabrication of self-ordered anodic alumina nanotemplate. FE-SEM analyses conducted after the high-field anodization, pulse detachment and chemical widening of pores showed the relationship of 2.2 nm/V in this voltage range, which was identical to the previously reported one in the literature. The growth rate of the alumina film was about 60 um/hr, which was 30 times faster than that of phosphoric acid mild anodization. This study provides a new process for the fast fabrication of nanotemplates with interpore distances larger than 300 nm.

• Korean Journal of Environmental Agriculture
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• v.13 no.3
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• pp.301-310
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• 1994

This experiment was conducted to test the fungicidal activity of extracts from 50 medicinal plants to powdery mildew (Sphaerotheca fulinginea) and identify the bioactive substances. Among the medicinal plants tested, the water extract of Rheum undulatum was the most effective in spore germination inhibition, which inhibited by 100% at 200-fold dilution. Also, 50-fold dilution of water extract, 100-fold dilution of alcohol extract, 500-fold dilution of crude extract from Rheum undulatum and even 1000-fold dilution of reference chemical inhibited powdery mildew of cucumber more than 60%. 500-fold dilution of crude extract inhibited powdery mildew of cucumber 100% by twice spray treatment. There was phytotoxcity at the 100-fold dilution, but was not recognized this injury at the 500-fold dilution of crude extract. From our research to identify bioactive substance using HPLC, GLC and Mass spectrum analysis, it indicated that Rheum undulatum extract contained tentatively 1,8-dihydroxy-3-methyl-9,10-anthracenedione and 1,8-dihydroxy-3-methoxy-6-methyl-9,10-anthracenedione.

• Journal of Energy Engineering
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• v.11 no.1
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• pp.18-25
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• 2002

One of the most serious concern in nuclear power plant piping maintenance is thickness reduction due to flow-accelerated corrosion (FAC). Since the FAC occurs under specific conditions of pH, dissolved oxygen, temperature, flow velocity, steam quality of the fluid and materials and geometry of the piping, a systematic approach is required for managing the FAC problem. In this study, construction of a secondary piping database, analyzing the FAC rate using the database and predicting the residual life was performed for a domestic CANDU nuclear power plant. Also FAC mechanism and factors affecting FAC were reviewed. By showing a case study on analysis for a pipe line between a separator and a flash tank, a procedure for managing FAC problem is suggested. The procedure proposed in this paper can be widely applied to the secondary piping of other domestic nuclear polder plants.

• 한국신재생에너지학회:학술대회논문집
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• 2008.05a
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• pp.210-213
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• 2008

The purpose of this study is to investigate the performances of organic removal and methane recovery in the full scale two-phase anaerobic system. The full scale two-phase anaerobic system was consists of an acidogenic ABR (Anaerobic Baffled Reactor) and a methanognic UASB (Upflow Anaerobic Sludge Blanket) reactor. The volume of acidogenic and methanogenic reactors is designed to 28.3 $m^3$ and 75.3 $m^3$. The two-phase anaerobic system represented 60-82% of COD removal efficiency when the influent COD concentration was in the range of 7,150 to 16,270 mg/L after screening (average concentration is 10,280 mg/L). After steady-state, the effluent COD concentration in the methanogenic reactor showed 2,740 $\pm$ 330 mg/L by representing average COD removal efficiency was 71.4 $\pm$ 8.1% when the operating temperature was in the range of 19-32$^{\circ}C$. The effluent SCOD concentration was in the range of 2,000-3,000 mg/L at the steady state while the volatile fatty concentration was not detected in the effluent. Meanwhile, the COD removal efficiency in the acidogenic reactor showed less than 5%. The acidogenic reactor played key roles to reduce a shock-loading when periodic shock loading was applied and to acidify influent organics. Due to the high concentration of alkalinity and high pH in the effluent of the methanogenic reactor, over 80% of methane in the biogas was produced consistently. More than 70 % of methane was recovered from theoretical methane production of TCOD removed in this research. The produced gas can be directly used as a heat source to increase the reactor temperature.

• Journal of Korean Society of Transportation
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• v.20 no.3
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• pp.55-67
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• 2002

구간검지체계를 기반으로 한 첨단교통정보제공시스템(Advanced Traveler Information Systems)은 그 기능 수행시 다음의 중요 고려사항을 지닌다. 첫째는 제공 정보의 신뢰성이며, 둘째는 정보수집비용에 관련한 수집자료수의 한계이다. 본 논문에서는 이러한 한계성 극복을 위해 보다 대표성 있는 교통정보 형태의 설정 및 통계적으로 신뢰성 있는 정보산출을 위해 요구되는 적정표본수의 결정에 대한 연구를 수행하였다. 도시고속도로(올림픽대로)와 도시간선도로(천호대로)의 실측 구간통행시간분포 분석결과 단일교차로 구간의 경우 다른 구간들의 단일봉(unimodal)의 정규분포형태와는 다른 두 개의 봉우리를 지닌 분포형태(bimodal)가 나타났다. 따라서 이러한 구간은 기존과는 다른 새로운 교통정보 형태가 필요하며, 본 논문에서는 모든 통과차량들의 평균통행시간으로 정의되는 한 개의 대표치가 아닌 신호주기에 의한 정지여부에 따라 분리되는 주행시간과 지체시간 또는 주행속도와 통행속도 개념의 세분화된 정보형태를 설정하였다. 또한 중심극한정리를 기초로 한 통계적인 표본수 결정식을 이용하여 설정된 신뢰수준 하에서의 정보산출을 위해 요구되는 적정 표본수를 산출하였다. 그 결과, 교통이 혼잡할수록 요구되는 표본수는 적어지는 것으로 나타났다. 우선 적정 표본수 만큼의 표본추출을 하고 제안된 정보산출 방법에 의해 교통정보를 산출한 후 실측치와의 오차를 비교하였다. 그 결과 산출된 교통정보는 신뢰수준 95%와 허용오차 5㎞/h를 만족하였다. 다음으로 구간검지체계를 이용하여 정보를 산출하는 타시스템 교통정보와의 오차율을 비교하였다. 그 결과, 실측치와 본 연구의 산출방법에 의한 교통정보, 로티스교통정보 및 차량번호판 인식시스템의 교통정보와의 비교 결과 제안된 교통정보형태의 타당성을 볼 수 있었다.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.4
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• pp.49-56
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• 2003

The strain Ralstonia eutopha JMP134 (formerly Alcaligenes eutrophus JMP134) can degrade trichloroethylene(TCE) through a chromosomal phenol-dependent pathway. The phenol hydroxylase was previously found to be a single responsible enzyme for TEC degradation. Here, we demonstrate that a recombinant bacterium, R. eutopha AEK301, one of Tn5-induced mutants of JMP134 containing a recombinant plasmid pYK3011, degrades TCE in the absence of inducer, phenol and in the presence of various carbon sources. Complete removal of TCE ($50{\mu}M$) was observed in minimal medium containing only 0.05% ethanol as a carbon source within 24 hours. The bacterium removed $200{\mu}M$ of TCE to below detectable level within two days under non-selective pressure. When TCE concentration was increased up to $400{\mu}M$, the degradation had been continued until two days, then ceased with removal of 70% of detectable TCE.

• 한국HCI학회:학술대회논문집
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• 2007.02a
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• pp.744-749
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• 2007

물체 추적시스템은 비디오 감시 시스템, 화상회의 시스템과 같은 다양한 비전 응용 분야에서 점점 비중이 높아지고 있다. 이 시스템에서 가장 널리 사용되고 있는 방법 중 하나로 Particle-Filter를 들 수 있다. 하지만, 이 Particle-Filter의 단점은 유사한 여러 물체를 추적할 때에 그 물체들이 겹치거나 사라질 경우 정확한 추적을 하기 어렵다는 것이다. 이 단점을 극복하기 위해 많은 연구가 진행되고 있으며, 본 논문에서는 이 문제를 극복하기 위한 새로운 방법을 제안하고자 한다. 다중 물체 추적에서 빈번히 일어나는 문제는 두 가지로 요약할 수 있는데, 동일한 다중 물체가 부분적으로 엇갈리거나 다른 객체에 완전히 겹친 후 떨어질 때 한 물체를 중복하여 추적하는 문제(merge and split problem)와 이 때 분리되어 추적은 됐지만, 물체를 혼동하여 추적하는 문제(Labeling problem)이다. 본 논문에서는 이 러한 문제들을 풀기 위해 이미지 필드에서 보다 정확한 확률분포를 만들고, 이 확률분포의 신뢰성을 높이기 위해서 물체의 특징정보를 표현하는 몇 가지 방법을 제안한다. 전자의 문제는 두 가지 문제로 나누어 생각해 보았다. 첫째, 복잡환 환경에서의 분포를 찾아내는 것과 둘째, 추적 중인 물체를 잃어버릴 경우 새로운 샘플을 생성함으로써 나누어 보았다. 이 문제 중 첫번째는 K-means 클러스터링을 이용하여 유사한 물체가 주변에 퍼져 있을 때, 하나의 후보 위치가 아닌, K개의 후보 위치들을 만들어 내어 보다 정확한 추적이 가능하게 하였으며, 두 번째 문제는 추적 중인 물체가 다른 커다란 물체에 가려질 경우이다. 이 상황에서 샘플을 생성하는 방법은 지금까지 해왔던 간단한 환경에서의 생성 범위와는 다르게 넓게 해야 생성시켜야 한다. 이 때 샘플링의 수를 늘리지 않으면서, 최대한 정확하게 추적하기 위해서 동영상에서 물체의 모션을 이용한 모션 히스토그램을 얻어내고, 그 정보를 이용하여 샘플을 생성하는 위치를 조절함으로써 이 문제를 풀어 보았다. 그리고, 후자의 문제인 이미지 필드상에서 확률분포의 신뢰성을 높이기 위한 특징 정보는 기존에 많이 사용하던 칼라 히스토그램에 공간정보의 의미를 부여하는 칼라 히스토그램을 분할하는 방법과 SIFT에서 사용하는 방향정보와 크기정보를 사용했다. 이것들을 사용하여 보다 정확한 물체추적시스템을 다음과 같이 제안한다.

• Journal of Biosystems Engineering
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• v.12 no.1
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• pp.45-52
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• 1987

In order to improve the thermal efficiency of an internal combustion engine, various ignition timing control systems were examined and the best one was chosen. The parts used for the systems were a microcomputer system with DAS, 8 bit output port (D-FLIP FLOP), three types of isolation circuit, two types of ignition timing pulse generator, three types of switching circuit and two types of high voltage ignition circuit. Most systems did not operate well due to the effects of electromagnetic waves and surge currents occurring when the ignition began or ended with resulting high voltage. The best ignition timing control system was found to be the combination of (microcomputer system)-(ignition timing pulse generator using step motor position control pick-up)-(switching circuit using TR logic)-(high voltage ignition circuit using CDI).