• Korean Journal of Environment and Ecology
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• v.15 no.3
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• pp.247-256
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• 2001

The purpose of the present study is to prepare the conservation category to establish the Natural Ecosystem Conservation Area(NECA) in Korea. The authors prepared the conservation category for the NECA based on the previous relevant papers and reports. This draft of conservation category was examined by way of the case study. From this, the possible factors are introduced to prepare the final conservation category that can be applied directly in Korean situation for the conservation of natural areas. The present conservation category was prepared based on the possible data, while some factors of which do not possess the adequate raw data at the moment was removed. According to the reference analyses from the previous conservation categories reported in order to prepare the conservation category in the NECA, a total of 27 factors was assessed; 21 for rarity and 15 fur utility and diversity, 13 far size, for both naturalness and human influence, 13 for typicalness 12 and 10 for fragility. From the literature reviews, the categories which quantitatively assessed was 13: 9 for rarity, 8 far human influence, and 5 for size. From the 27 conservation categories assessed, the factors such as rarity, taxonomic distinctiveness and threat was regarded as applicable to the Korean situation. Furthermore, the criteria such as use could be applied further when this is supported by the augmentative research and field studies.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.246-247
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• 2000

수서동물 생식소의 해부학적 구조 및 생식세포의 미세구조는 종 특이성, 생식생태 및 계통에 따라 다르다. 일반적으로 이매패류의 정자는 각 분류군에 따라 핵과 첨체의 모양, 첨체의 axial rod, 미토콘드리아 수, 편모의 수 및 형태 등에서 다양한 특징을 보인다 (Morse and Zardus, 1997). Anadara trapez 등이 속하는 돌조개과 (Arcidae) 패류들의 정자 두부는 난형이며, 첨채에 axial rod가 없고, 중편에 4개의 미토콘드리아를 가진다. (중략)

• Parasites, Hosts and Diseases
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• v.27 no.1
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• pp.1-8
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• 1989

Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the purity of antigens used. To solve this problem. many workers have tried to find species-specific components of antigens, The present study was performed to determine the antigenic profile of crude saline extracts of 3, 5, 8 and 12-week old p. westermani worms, which were collected from experimentally infected cats, based on SDS-PAGE and immunoblot technique. The results were as follows: 1. The SDS-PAGE showed at least 30 Protein bands ranging from 229 kDa to 10 kDa molecular weight. The protein components of p. wsstermani changed chronologically during its developmental period. The 229 kDa band was recognized only in 12-week old worms ($$SEP_{l2}$). 2. Analysis by ELISA showed a significant increase in antibody levels at 3 weeks in infected cats using crude saline extract antigens ($SEP_3,{\;}SEP_5,{\;}SEP_8,{\;}SEP_{l2}$). 3. By EITB using $SEP_3$ and $SEP_5$ infected cats recognisea major protein bands with molecular weight of 60, 35, 28, 25 or 21 kDa at 3~12 weeks of infection, and 3 additional antigens, 19, 13 and 10 kDa, were detected at 8~12 weeks of infections. 4. Using $SEP_8$ 5 antigens, 91, 85, 31, 25 and 21 kDa, were consistently detected by all infected sera tested. In addition, 3 antigens of lg. 13 and 10 kDa were detected at 8~12 weeks of infection. Using $SEP_12$, similar results were obtained with that by using $SEP_8$ and 1 additional antigen of 229 kDa, specifically reacting with the sera from 12 weeks of in(traction, was recognized.

• The Microorganisms and Industry
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• v.27 no.1
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• pp.2-17
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• 2001

Cyclomaltodextrinase(CDase, EC 3.2.1.54), maltogenic amylase(EC 3.2.1.133). neopullulanase(EC 3.2.1.135)는 cyclomaltodextrin(CD), pullulan 및 전분을 가수분해하는 효소들이다. 이 효소들은 $\alpha$-1,4-Ο-glycosidic 결합에 작용하여 CD와 전분을 말토오스로 pullulan을 panose로 가수분해할 뿐만 아니라 올리고당들을 다양한 당 수용체 분자들의 C-3, C-4. C-6 수산기로 전이시키는 활성도 갖고 있다. 이러한 특성들은 기존의 $\alpha$-amylase를 비롯한 판수화물 분해효소들과 뚜렷이 구별되는 것으로 전분 분해효소들의 분류체계에 새로운 기준점을 제시한다고 하겠다. 본 총설에서는 CDase, maltogenic amylase, neopullulanase처럼 pullulan이나 전분보다 CD를 훨씬 더 잘 분해하는 효소들과 Thermoactinomyces vulgaris amylase II(TVA II)처럼 CD를 분해하기는 하나 pullulan을 더 잘 분해하는 효소들의 생화학적, 효소적, 구조적 특성들을 종합하여 소개하고자 하였다. 이 효소들은 40~60% 정도로 아미노산 서열이 동일하고, 세포 내에 존재하며, 분자량이 62~90 kDa로 $\alpha$-amylase보다 다소 크다. 아미노산 서열 비교분석 및 maltogenic amylase와 TVA II 등의 3차구조 분석 결과, 이 효소들은 아미노 말단에 보통 $\alpha$-amylase에는 존재하지 않는 약 130개 아미노산으로된 영역을 갖고 있어 이를 매개로 이합체를 형성할 수 있는 것으로 나타났다. 이합체-단위체 평형은 염 농도, 효소 농도, 산도 등에 의해 조절되고 단위체와 이합체 모두 효소환성을 갖고 있으나, 기질 특이성이 다르며 단위체는 전분을, 이합체는 CD를 선호하는데 이는 이합체 형성 시 활성부위의 구조적 변화에 따른 것으로 분석되었다. 본 총설에서는 CD 분해효소들의 다양한 기질 특이성을 올리고머 형성 등의 구조적 특성과 관련하여 논함으로써 관련 효소들의 분류체계를 보다 명확히 할 수 있는 자료를 제공하고자 하였으며, 이러한 효소들의 생리적 기능 및 산업적 이용에 대해 제안하고자 하였다.

• Journal of Life Science
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• v.27 no.11
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• pp.1331-1339
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• 2017

DNA barcoding is the identification of a species based on the DNA sequence of a fragment of the cytochrome C oxidase subunit I (COI) gene in the mitochondrial genome. It is widely applied to assist with the sustainable development of fishery-product resources and the protection of fish biodiversity. This study attempted to verify horse-head fish (Branchiostegus japonicus) and fake horse-head fish (Branchiostegus albus) species, which are commonly consumed in Korea. For the validation of the two species, a real-time PCR method was developed based on the species' mitochondrial DNA genome. Inter-species variations in mitochondrial DNA were observed in a bioinformatics analysis of the mitochondrial genomic DNA sequences of the two species. Some highly conserved regions and a few other regions were identified in the mitochondrial COI of the species. In order to test whether variations in the sequences were definitive, primers that targeted the varied regions of COI were designed and applied to amplify the DNA using the real-time PCR system. Threshold-cycle (Ct) range results confirmed that the Ct ranges of the real-time PCR were identical to the expected species of origin. Efficiency, specificity and cross-reactivity assays showed statistically significant differences between the average Ct of B. japonicus DNA ($21.85{\pm}3.599$) and the average Ct of B. albus DNA ($33.49{\pm}1.183$) for confirming B. japonicus. The assays also showed statistically significant differences between the average Ct of B. albus DNA ($22.49{\pm}0.908$) and the average Ct of B. japonicus DNA ($33.93{\pm}0.479$) for confirming B. albus. The methodology was validated by using ten commercial samples. The genomic DNA-based molecular technique that used the real-time PCR was a reliable method for the taxonomic classification of animal tissues.

• Korean Journal of Plant Taxonomy
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• v.51 no.3
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• pp.250-293
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• 2021

Limestone areas are sedimentary rock outcrops consisting of calcium carbonate created several hundreds of millions of years ago by calcium-secreting marine organisms and subsequently lifted above sea level by tectonic movement. Limestone areas support very high levels of endemic species of plants and are recognized as biodiversity areas with much biological information. The purpose of this study is to devise a strategy for the comprehensive conservation of the vegetation of limestone areas through analyses of the floristics and plant species compositions in ten limestone areas on the Korean Peninsula. The results of 153 field surveys from April of 2010 to October of 2016 identified 1,202 taxa in total, representing 1,096 species, 18 subspecies, 84 varieties, 2 forms, and 2 hybrids in 530 genera and 133 families. Among them, 55 taxa were endemic plants to Korea, and 38 taxa were red data plants. The floristic target plants amounted to 102 taxa, specifically 27 taxa of grade V and 75 taxa of grade IV. In all, 121 alien plants were recorded in the investigated area. Calciphilous plants amounted to 102 taxa, specifically 14 taxa of calciphilous indicator plants, 30 taxa of superlative most calciphilous plants, and 58 taxa of comparative more calciphilous plants. A cluster analysis showed a high degree of similarity between sites that are geographically adjacent with similar habitat environments. Limestone areas also supported groups distinct from those in non-limestone areas, demonstrating the specificity of limestone flora. Plant geography approaches therefore appear to be crucial to gain a better understanding of the level of biodiversity in limestone areas, not only at the interspecific but also at the intraspecific level. These results highlight the importance of protecting limestone habitats to preserve not only their interspecific but also the intraspecific diversity, which is highly threatened.

• Korean Journal of Environment and Ecology
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• v.28 no.2
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• pp.161-170
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• 2014

This study classified the types of major forest vegetation and analyzed the syngeographical characteristics of the vegetation in the Mt. Deokhang with exhumed lime rocks. The forest vegetation on the ridge of Mt. Deokhang was classified into 1 community group, 3 communities, 2 groups, and 2 subgroups, 5 types in total. The syngeographical form of Deokhangsan fully exhibited the topographical peculiarity, proximity to subalpine, and limestone zone. In addition, Carex ciliato-marginata, Carex humilis var. nana, and so forth which appear as species with high consistency in the well-drained semiarid zone represent the soil environment of the lime stone area and northern plants such as Carex ussuriensis and Asplenium ruta-muraria represent the characteristics of the vegetation in alpestrine in close proximity. Moreover, the Carpinus turczaninowii in the area appears to show the characteristics of migratory vegetation due to the peculiarity of the habitat. The syngeographical characteristics of this study area show both the characteristics of the habitat and phytogeographic characteristics. Furthermore, they are expected to contribute to the diversity in community and habitat form in Korean peninsula in terms of vegetation science.

• The Korean Journal of Mycology
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• v.24 no.3 s.78
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• pp.228-236
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• 1996

Flesh fungi were collected during field trips to mountain areas throughout the country from May to October of 1994. Through the observation and identification of specimens belonging to the Aphyllophorales, one genus, Tylospora, and six species, Athelia fibulata, Hypochnicium punctulatum, Tylospora fibrillosa, Stereum ochraceo-flavum, Steccherinum litschaueri, and Oligoporus undosus were confirmed new to Korea and are registered here with descriptions.

• Korean Journal of Environment and Ecology
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• v.26 no.1
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• pp.11-38
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• 2012

Located in the middle part of the Korean peninsula, Pyeong-chang and Yeong-wol (Gangwon-do) areas are limestone regions that are important biogeographically showing the peculiar flora. In order to manage efficiently endangered and rare plant species, we have investigated flora of these above regions from March to October, 2010. The result of the investigation showed a total of vascular plants of 1,145 taxa; 134 families, 503 genera, and 1,010 species, 11 subspecies, 110 varieties, 10 forms and four hybrids. Among taxa investigated, endangered species designated by the law called 'Protection Law for Endangered wild fauna and flora' were seven taxa, and 50 taxa were considered to be endemic to Korea. Based on the floristic degrees categorized by the specific distribution of plant species, 24 taxa revealed the floristic degree (FD) V, 30 for FD IV, and 60 for FD III, 45 for FD II and 79 taxa for FD I. The 82 taxa were identified to be naturalized. Of those species investigated in this survey, 53 taxa were identified as specific ones biogeographically as compared to flora of other terrestrial regions; 35 of calcicolous plants, 15 with distribution in the northern part of the Korean peninsula, three with coastal habitats.

• Korean Journal of Ichthyology
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• v.9 no.1
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• pp.121-129
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• 1997

The five species of genus Cobitis from Korea were investigated by electron microscopes to clarify the adhesive membranes on zona radiata. In the late vitellogenic stage the adhesive membranes could be classified into two form as follows: 1) granular form of Cobitis lutheri, C. striata, C. sinensis, and C. sinensis-longicorpus, 2) villous form of C. melanoleuca. Although C. lutheri, C. striata, C. sinensis, and C. sinensis-longicorpus possessed the same granular form, it was evident that fine structure of the zona radiata varied according to species. The adhesive membranes and fine structures of zona radiata in Cobitis showed a species specificity closely related to their habitats and spawning properties.