• Title/Summary/Keyword: 분광광도법적

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The Spectrophotometric Determination of Reducing Sugar in the Tobacco Leaves by losing a Color Developing Reagent, Blue Tetrazolium Chloride. (발색시약 Blue Tetrazolium Chloride를 이용한 잎담배중 환원당의 비색법적 정량.)

  • Lee, Mun-Su;Kim, Sin-Il
    • Journal of the Korean Society of Tobacco Science
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    • v.2 no.2
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    • pp.38-43
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    • 1980
  • The spectrophotometric determination oi reducing sugar in the tobacco leaves was carried out by employing blue tetrazolium chloride as a color developing reagent. The absorption maximum of formazan dye which is the product of the reaction between blue tetrazolium chloride and fructose was shown to be $530^{nm}$. In the fructose concentration range of 0.02mg/ml-0.14mg/ml the calibration curve was well coincided to the law of Beer-Lambert. In order to take a look at the accuracy and/or recovery rate of fructose determination, the standard fructose was added to the tobacco leaves and the concentration of this standard fructose was estimated. A tittle lower concentration of the standard fructose compared with the pure one in solution was observed. However, an excellent analytical recovery was revealed under the -2% of relative error limit. When we carried out the quantitative determination of this reducing sugar by the method of visual read-out (without using the spectrophotometer), the relative error was obtained to be $\pm$10%.

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