• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.127-140
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• 2006

Purpose : This study was conducted to investigate the antimicrobial effects of Astringent medicinals against vaginal microbe. Methods : Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Candida albicans and Gardnerella vaginalis were used for vaginitis-induced microbe. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for normal vaginal florae. And Astringent medicinals (Rubi Fructus, Tritici Immatri Semen, Corni Fructus, Nelumbinis Semen, Mume Fructus, Schizandrae Fructus, Ailanthi Radicis Cortex, Galla Rhois, Myristicae Semen, Terminariae Fructus, Rosae laevigatae Fructus, Ephedrae Radix) were used in this study. Antimicrobial activities were tested by optical density and colony test in vitro. And then wee valuated the antimicrobial effects in comparison with optical density and colony test. Results : The optical density and colony test showed that Terminariae Fructus and Galla Rhois among Astringent medicinals had the antimicrobial effects. Teminariae Fructus had the antimicrobial susceptibility and selective toxicity against Candida albicans and Gardnerella vaginalis of vaginitis-induced microbe. Galla Rhois had the antimicrobial susceptibility and selective toxicity against Staphylococus aureus, Methicillin-resistant Staphylococus aureus, Candida albicans and Gardnerella vaginalis of vaginitis-induced microbe. Conclusion : According to these results, we can suggest that Terminariae Fructus and Galla Rhois among Astringent medicinals be available to the antimicrobial agent of vaginitis-induced microbe in vitro.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.34-48
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• 2006

Purpose : This study was conducted to investigate the inhibitory effects of Dangguijakyaksan on cell proliferation in HeLa cells. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Dangguijakyaksan extract solution for 24 hours, 48 hours and 72 hours for the direct inhibitory effects of Dangguijakyaksan. Afterwards, we executed the analysis of the effect of Dangguijakyaksan extract solution on cell proliferation inhibition using XTT assay, molecular biological method through MAP kinase activity and FACS analysis of caspase activity in the HeLa cells. Results : After 24, 48 and 72 hours cultivation, Dangguijakyaksan extract solution group showed significant decrease of HeLa cells except 1% solution after 24 hours compared with the control group. In the FACS analysis, Dangguijakyaksan extract solution groups showed increase of caspase activity except 1% solution after 48 hours compared with the control group. In the XTT assay, the caspase-3 activities were increased in Dangguijakyaksan extract solution groups except 1% solution after 24 hours in a dose-dependent manner. In the XTT study, cell activities were significantly decreased in 10% Dangguijakyaksan extract solution groups after 48 and 72 hours cultivation compared with the control group. In all Dangguijakyaksan extract solution groups, The activities of MAP kinase were decreased after 24, 48 and 72 hours cultivation compared with the control group. Conclusion : It could be concluded that Dangguijakyaksan is available to the inhibition of proliferation of human cervical carcinoma cell line in vitro.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.1
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• pp.139-154
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• 2006

Objective : This experimental study was carried out to investigate anti-coagulation effects and mechanism of Hongdeung-tang on the dissentimenated intravascular coagulation Methods : Each 10 rats were classified as normal gorup, control group and treated group. The control group was given a 0.5mg/ml endotoxin intra-vein injection and was occurred Disseminated Intravascular Coagulation(DIC). The treated group were orally administrated 1ml Hongdeung-tang concentration once a day for 6 days and then it was treated the same as the control group. We took the blood from heart and were investigated number of Platelet and white blood cell(WBC), fibrinogen concentration, prothrombin time(PT), activated partial thromboplastin time(APTT), interleukin-6(IL-6), Tumor Necrosis $Factor-{\alpha}(TNF-{\alpha})$, Prostaglandin $E2(PGE_2)$ from them. Results : There were a statistically significant increase of platelet and fibrinogen concentration in the treated group compared to the treated group. The PT was a significantly reduced in treated group compared to the control group. There was no significant change in numbers of WBC and APTT between control and treated group. On the contrary, the number of PT, IL-6, $TNF-{\alpha}$, $PGE_2$ were significantly decreased in treated group compared to the control group. Conclusion : DIC which was occurred by endotoxin intravenous injection seems to related to the increase of IL-6, $TNF-{\alpha}$, $PGE_2$ and Hongdeung-tang has an effect that supress those factors. we can assume Hongdeung-tang has an anti-coagulation effect by those supressing effect.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.18-33
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• 2006

Purpose : Endometriosis has been thought to be related with blood stasis in uterus. Onkyung-tang is a herb which has effectiveness of activating blood, warmy and regulating mensturation, therefore in the present study, the effects of Onkyung-tang on endometriosis were investigated. Methods : The endometrial tissue was autografted to Rat's small intestine. Rats with surgically induced endometriosis was orally administerd with Onkyung-tang for 40 days. Size of ectopic uterine implants at the serosal wall and concentration of progesterone, estradiol, $TNF-{\alpha}$ and IL-2, 4, 6, 10 in serum were examined and compared with the control group. Results : The size of ectopic uterine implants of treated group was much smaller than that of control group. The concentration of estradiol, $TNF-{\alpha}$ and IL-4 level were significantly decreased in experimental group compared with the control group. IL-10 level was significantly increased in experimental group compared with the control group. There was no significant difference in progesterone, IL-2, 6 level in experimental group and control group as well. Conclusion : My data suggest that Onkyung-tang reduce the size of ectopic uterine implants at the serosal wall and inhibit the growth of ectopic uterine implants. Judging from the above results, it can be suggested that Onkyung-tang should be a useful agent for inhibiting the proliferation of uterine endometrial tissue.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.228-237
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• 2002

Purpose This study was designed to know how Hyunburikyungtang-gagam effects on dysmenorrhea Method 47 patients visited Oriental Gynecology in Conmaul Oriental Hospital from Jan, 2001 to Jan. 2002 because of their dysmenorrhea were randomly assigned. Hyunbruikyungtang-gagam were prescribed to the patients. Primary and secondary dysmenorrhea change were studied by VASS, MVRS. Written down by the patients before and after the treatment. Results Their ages were $28.62{\pm}4.9$, 18 patients were married and the others were not married. prescription period were $20.59{\pm}0.97$ days, After the treatment, numerical index for the pain was obviously reduced in both primary and secondary dysmenorrhea. Numerical index reduced $5.43{\pm}1.63$(VASS), $3.04{\pm}1.58$(MVRS) in primary dysmenorrhea, and $5.43{\pm}1.63$(VASS), $3.04{\pm}1.58$(MVRS) in secondary dysmenorrhea and there is no difference in effect between primary and secondary dysmenorrhea. But prescribed period was not correration with treatment effects and Pain-reduced period. Conclusion Hyunburikyungtang-gagam has good efficacy on primary and secondary dysmenorrhea, but some more studies are needed on the relationship between treatment effects and Pain-reduced period.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.193-201
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• 2002

Purpose : Premature ovarian failure, characterized by amenorrhea or oligomenorrhea, estrogen defieciency and elevated gonadotropins in woman less than 40 years of age, affects approximately 1% of woman and is associated with autoimmune phenomena or genetic abnormalities, but mostly idiopathic. This disease is loss of ovarian function seems not to be always permenent. Method : We investigated herb medication was effective in patient of premature ovarian failure. Results: Signs and symtoms of estrogen deficiency were improved and mensturation occurred monthly and the elevated during treatment of herb medication. As a result of our case recovering of ovarian function, we hope more investigtions about treating premature ovarian failure by oriental medicine will be gained.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.32-44
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• 2002

Onsinhwan(OSH : 溫腎丸) is used in female infertility, especially due to lack of kidney yang or Sinyanghe(腎陽虛). An attempt was made to evaluate the influences of OSH on the serum concentrations of FSH and LH, Estrogen, Progesterone and the histological and optical changes of ovary, ovary weight of rats. The results of the study were as follows : 1. Blood FSH level increased in experimental group as compared with control group on 7th day and 14th day, which showed no efficacy. 2. Blood LH level decreased in experimental group as compared with control group on 14th day, which showed no efficacy. 3. Blood Estrogen level increased in experimental group as compared with control group on 7th day and 14th day, which showed no efficacy. 4. Blood Progesterone level decreased in experimental group as compared with control group on 7th day and 14th day, which showed no efficacy. 5. In histological observations of ovary, ovulation increased in experimental group as compared control group on 7th and on 14th day, which showed no efficacy. 6. In optically observations of ovary, size and weight of ovary increased in experimental group on 7th day and on 14th day, which showed no efficacy. According to these results, OSH did not influence on the ovulation of ovary in rats.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.17-31
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• 2002

Objective : This study was carried out to investigate the effects of the hormones and cytokines associated with bone metabolism in ovariectomized rats. Method : Twenty-four Female-Sprague-Dawley rats were divided into sham operated(normal) group, ovariectomized(control)group, ovariectomized and treated with extract Caraganae Sinicae Radix(treated) group. Each group was investigated the changes of body weight at 3,5,7weeks after treatment, and femur weight, femur/body weight, thickness of compact bone of body of femur, area of cancellous bone of distal epiphysis of femur, serum estrogen, serum calcitonin, serum parathormone, serum Tumor Necrosis $Factor-{\alpha}$, serum $lnterleukin-1{\beta}$ at 7weeks after treatment. The results were summerized as follows; 1. The treated group showed significant change in body weight compared with the control group at 5,7weeks after treatment. 2. The treated group revealed significant increases in femur/body weight compared with the control group. 3. The treated group showed a little thicker compact bone of body of femur than the control group. 4. The area of cancellous bone of distal epiphysis of femur in treated group was increased significantly compared with control group. 5. The level of serum estrogen showed no change compared with control group. 6. The level of serum calcitonin showed no change compared with control group. 7. The level of serum parathormone was decreased in treated group significantly compared with control group. 8. The level of serum $TNF-{\alpha}$ was decreased in treated group significantly compared with control group. 9. The level of serum $interleukin-1{\beta}$ showed no change compared with control group. The results indicate that Caraganae Sinicae Radix inhibits bones resorption in ovariectomized rats by it's lowering effects on serum parathormone and $TNF-{\alpha}$.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.149-162
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• 2002

The fetus is greatly influenced by mother's health condition and her environment. So in our traditional society, the prenatal care is regarded as important for generations to birth of sound baby in mind and boy. Purpose : We aimed to reserch what kind of mother's conditions can be more influential to infant allergic disease among traditional prenatal care. Method : We investigated 91 patients who answered by a questionnaire at Dept. of Pediatrics, Oriental Hospital of Dae-jeon University. We divided patients into two groups, A(normal group;43) and B(allergic disease group;48) and analyzed those. Results : The pregnant women in the group B, generally have worse health conditions and are under the stress mentally and cannot get enough nutrition because of morning sickness and digestive trouble, but take more pungent and powdered food than the group A. Specially, the result is significant in anger and worry among other mental stresses and in digestive trouble and threatened abortion among other health conditions(according to order: p<0.001, p<0.05, p<0.01, p<0.001). We can see that pregnant woman's peaceful mentality and enough nutrition intake are most important to birth of sound baby in mind and also we can see that pregnant woman's regimen has influence on not only infant disease but also character and the infant allergic diseases are related to inheritable factor.

• The Journal of Korean Obstetrics and Gynecology
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• v.15 no.4
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• pp.183-192
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• 2002

Objectives The Objective was to evaluate the efficacy of auricular acupuncture treatment for adolescent dysmenorrheic women. Method This study was carried out to the 25 adolescent dysmenorrheic women without pelvic or other systemic disease and its history who visited outpatient department of Oriental Hospital, Semyung, univ. from June, 2002 to September, 2002. We investigated general characteristics, menstrual and dysmenorrheic characteristics through out the patient's note and comparison of before and after auricular acupuncture treatment by using a 10cm Visual Analogue Scale(VAS) for sample patients. Results The $mean{\pm}Standard$ Error of mean(S.E) of VAS score on before and after auricular acupuncture treatment were $6.36{\pm}0.49$ and $2.24{\pm}0.35$. The $mean{\pm}S.E$ of VAS score of after auricular acupuncture treatment was decreased significantly compared with that of before(p<0.01). Conclusion The auricular acupuncture treatment will be efficacious against adolescent dysmenorrhea