• 대한한방부인과학회지
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• 제23권4호
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• pp.47-56
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• 2010

Purpose: This study was performed to evaluate the single dose oral toxicity of Gwibi-tang extract in ICR mice. Methods: 0(control group), 1250, 2500 and 5000 mg/kg of Gwibi-tang extracts were orally administered to 20 male and 20 female ICR mice. After single oral administration of Gwibi-tang extract to ICR mice, we observed number of the death, clinical signs, changes of body weights for 14 days. After 14 day of Gwibitang extract administration, all mice were sacrificed and major organs were observed. Results: Compared with the control group, we could not find any toxic signs in the mortalities, clinical signs, body weight changes, necropsy findings and hematological values in all treated groups(1250, 2500 and 5000 mg/kg). Conclusions: $LD_{50}$ value of Gwibi-tang extracts may be over 5000 mg/kg and it may have no side toxic effect to ICR mice.

• 대한한방부인과학회지
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• 제23권4호
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• pp.164-175
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• 2010

Purpose: The purpose of this study is to report the effect of Traditional Korean Medicine therapy for patient in the state of post stillbirth by oligohydramnios Methods: The patient in this case was 33-year-old female who was pregnant by artificial insemination in 2010. Intrauterine growth restriction was discovered at 17 weeks after gestation. And oligohydramnios was found at 20 weeks after gestation. Amnioinfusion was done three times, amniotic fluid infection occurred in the process of amnioinfusion and she had a stillbirth. After stillbirth she came to our clinic with symptoms of abdominal tenderness, distention, mammalgia. The patient was treated by traditional herbal medicine therapy such as Saenghwa-tang(生化湯), Gungguijohyeol-eum(芎歸調血飮), Silso-san(失笑散) and Sasang constitution medicine(荊防地黃湯, 荊防敗毒散, 荊防瀉白散). The progresses of symptoms were evaluated by visual analogue scale. Results: After Traditional Korean Medicine therapy, the patient's symptoms caused by stillbirth was improved. Conclusion: This case study shows that the postpartum treatment in Traditional Korean Medicine therapy and Sasang constitution medicine is effective for the patient in the state of post stillbirth.

• 대한한방부인과학회지
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• 제23권4호
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• pp.80-94
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• 2010

Purpose: This survey was done in order to determine Korean medical doctors (K.M.D)' expertise on clinical practice guideline for infertility and the degree of concordance with the guideline. Methods: The questionnaires were distributed via e-mail to all members of Korean Medical Doctors Society and Korean Gynecology Society from August $30^{th}$ to September $1^{st}$ in 2010. Two hundred sixty nine of completed questionnaires were analyzed through two groups, members of the Gynecology Society and nonmembers of the Gynecology Society. Results: According to the analysis of the outcome of the survey done by 99 members of the Korean Gynecology Society and 170 non-members, two-thirds of the participants had prior information of the clinical practice guideline. 63.6 percent of the participants feel strong necessity of the development of Korean medical infertility clinical practice guideline. Seventy percent of the participants are willing to participate in developing or reviewing a clinical guideline. On the question in active utilization and concordance to the guideline once the Korean medical infertility guideline is developed, 44% answered they would while 34.6% said they would utilize the guideline after some modifications. On the prospect of the impact of the development and application of Korean medical clinical practice guideline for infertility, majority of the participants marked positively that the standardization of clinical practice for infertility will lead to the nation's participation with the Nation's Support Work and that the development will somewhat contribute to the standardization of the clinical practice. Conclusions: The survey confirmed high demand of the clinical practice guidelines for infertility and the degree of concordance for infertility amongst Korean Medical doctors.

• 대한한방부인과학회지
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• 제21권1호
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• pp.117-133
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• 2008

Purpose: This study was performed to evaluate anti-thrombotic effects of Hyulbuchukeotanggamibang(HBCT). Methods: It was measured the effects which was given to blood flow rate through the regular volume of glass tube after the blood was diluted five times with ACD soulution. Antithrombotic effect was calculated as a percentage of the experimental animal figure protected from the paralysis of hind legs or death of the mouse that is caused from the administration of platelet aggregation regent. Results: 1. HBCT showed a safety in toxicity of liver. 2. In experiment of anti-thrombotic effect, HBCT inhibited the platelet aggregation induced by ADP and epinephrine, collagen, arachidonic acid as compared with the control group. 3. HBCT inhibited pulmonary embolism induced by collagen and epinephrine (inhibitory rate is 50%). 4. HBCT increased platelet number and fibrinogen amount significantly and also HBCT shortened PT and APTT significantly as compared with the control group in thrombus model induced by dextran. 5. HBCT increased blood flow rate insignificantly as compared with the control group in vivo. Conclusion: These results suggest that HBCT can be used for treating diverse female diseases caused by thrombosis.

• 대한한방부인과학회지
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• 제21권1호
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• pp.216-230
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• 2008

Purpose: The purpose of this study is to analysis on the Stress Response Inventory(SRI) and Heart Rate Variability (HRV) of some dysmenorrhoea patients. Methods: We studied 34 patients visiting OO Medical Center from May 2007 to November 2007. And the severity of dysmenorrhoea were measured by Multidimensional Verbal Rating scale(MVRS), Verbal Rating scale (VRS) and Visual Analog Scale(VAS). And SRI and HRV were recorded. Results: According to comparison of Means of HRV values among the groups, most of them were not statistically significant. But the comparison of Means of SRI, the points of almost all values were kept by order of Severe-Moderate-Mild-None group. Most of them are statistically significant. According to correlation analysis between dysmenorrhoea scale and HRV, most of them were not statistically significant. But the correlation analysis between HRV and SRI, there were some correlations. And the correlation analysis between SRI and dysmenorrhoea scale, most of them were statistically significant. According to General Linear Model of Dysmenorrhoea $scale^{\ast}SRI$ and HRV. Dysmenorrhoea scale and SRI influenced values of HRV. But it is hard to conclude the relationship between Dysmenorrhoea $scale^{\ast}SRI$ and HRV Conclusion: The results suggest that stress is related to dysmenorrhoea. But the relation between dysmenorrhoea and HRV was hard to conclude. We need more study for settling the relation between dysmenorrhoea and HRV.

• 대한한방부인과학회지
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• 제21권1호
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• pp.231-241
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• 2008

Purpose: This study estimated HRV and CBC of Qi deficiency-risky group and non-risky group at the seventh day after parturition, and found out their correlation with autonomic nervous system and immunity for the purpose of expressing diagnosis of Oriental Medicine objectively. Methods: The subjects of this study were 33 postpartum women who admitted for postpartum care in Oriental Hospital of Woosuk University from 17th July 2007 to 15th August 2007. We made inquiries based on Qi-Hyul-Su chart of Terasawa Katsutoshi and tested their HRV and CBC. Results: 1. The average age of Qi deficiency-risky group was higher than non-risky group and showed statistical nonsignificances. 2. Normalized LF was higher in Qi deficiency-risky group than non-risky group and Normalized HF was higher in Qi deficiency-non-risky group than risky group, both showed statistical significances. 3. WBC is higher in Qi deficiency-risky group than non-risky group showed statistical significances. WBC and Qi deficiency score had positive correlation. 4. LYM was higher in Qi deficiency-non-risky group than risky group and Grn was higher in Qi deficiency-risky group than non-risky group, both showed statistical significances. Conclusion: We found out that in Qi deficiency-risky group of postpartum women, immunity is lower than in non-risky group.

• 대한한방부인과학회지
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• 제21권1호
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• pp.242-256
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• 2008

Purpose: The purpose of this study is to find out the relation of oriental medicine examination(Inbody, HRV, PTG) and FSH, LH. Methods: We have carried out clinical trials who volunteered for Sasang constitutional medicine and Oriental OB & GY, Oriental Medical hospital of Dong-Eui University from November 2005 to January 2006. Women of Volunteers were 129 persons. Because 7 persons didn't exam oriental examination so we analyzed the results statistically for 122 persons. Results: 1. Inbody & FSH, LH In the relation of FSH correlation with results of Waist-Hip Ratio, Visceral Fat Area was increased(+direction), in the relation of LH correlation with results of Edema, Visceral Fat Area was increased (+direction). 2.The fluents of HRV & FSH, LH In the relation of FSH, LH correlation with results of SDNN, RMS, TP, VLF, LF, HF was decreased (-direction). 3.The fluents of PTG & FSH, LH In the relation of FSH, LH correlation with results of the fluents of PTG was decreased (-direction), with results of b_a was increased (+direction), with results of d_a was decreased (-direction). Conclusion: From this study, Inbody and HRV and PTG are corelation with FSH, LH.

• 대한한방부인과학회지
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• 제21권1호
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• pp.257-266
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• 2008

Purpose: These experiments were undertaken to evaluate the effect of Onpoeum on ovarian functions and differential gene expressions related with cell viabilities caspase-3, MAPK and MPG in female mice. Methods: We administered the Onpoeum to 6-week-old female ICR mice for 4, 8, or 12 days. With different concentration of Onpoeum, the female mice were injected PMSG and hCG for ovarian hyperstimulation. The mice divided into 3 different groups for each experiment. We chose the Caspase-3 for cell apoptosis, MAPK and MPG genes for cell viability and DNA repair. Results: In case of 4, 8, 12 day of Onpoeum, we were examined the mean number of total ovulated oocytes and the number of morphologically normal oocytes. We were also examined the embryonic developmental competence in vitro. In addition we were examined the differential expression of cell apoptosis, viability and DNA repair related genes, Caspase-3, MAPK and MPG according to concentration and duration of Onpoeum. From these results showed that the administration of Onpoeum played a role of prevention of cell apoptosis and DNA damages and also increased cell proliferation resulted in ovarian functions. Conclusions: It is suggested that the medication of Onpoeum may have beneficial effect on reproductive functions of female mice via prevention of cell apoptosis and DNA damaging and promotion of cell proliferation.

• 대한한방부인과학회지
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• 제21권1호
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• pp.16-27
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• 2008

Purpose: This study was conducted to investigate effects of Allii tuberosi Semen, Ginseng Radix Alba and Morindae officinalis Radix extract on reproductive capacities in mice. Methods: The 8-week-old ICR rats are used in the study, and we administered the extract solution of Allii tuberosi Semen, Ginseng Radix Alba and Morindae officinalis Radix concentration of 1, 10, 100mg/0.3ml to each rats and water to another rats(control group) once a day for each 30, 60, 90 and 120 days. we evaluated the total sperm count and morphology. Results: For 30 days, the total sperm count and normal sperm most increased in the group administered by 100mg/0.3ml Allii tuberosi Semen. For 60 days, the total sperm count and normal sperm most increased in the group administered by 100mg/0.3ml Morindae officinalis Radix solutions. For 90 days, the total sperm count most increased in the group asministered by 10mg/0.3ml Morindae officinalis Radix and 100mg/0.3ml Allii tuberosi Semen solutions. For 120 days, the total sperm count and normal sperm most increased in the group administered by l0mg/0.3ml Ginseng Radix Alba. Conclusion: This study shows that there were dose and duration effects of Allii tuberosi Semen, Ginseng Radix Alba and Morindae officinalis Radix extract solution on the total sperm count and morphology.

• 대한한방부인과학회지
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• 제21권1호
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• pp.39-54
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• 2008

Purpose: The purpose of this study was to investigate the anti-inflammatory effects of the water extract of Omisodokeum (OMSDE) on peritoneal macrophages, Methods: To verify the anti-inflammatory mechanism of OMSDE, the activation of nuclear $factor-{\kappa}B$ $(NF-{\kappa}B)$ and the phosphorylation of MAPK were examined. Results: The extract of OMSDE suppressed the production of LPS-induced nitric oxide (NO), tumor necrosis factor $(TNF)-{\alpha}$, interleukin $(IL)-1{\beta}$, IL-6 and IL-12 in the macrophages. OMSDE inhibited the degradation of inhibitory ${\kappa}B-{\alpha}$ $(I{\kappa}B-{\alpha})$ and it suppressed the activation of extracellular signal-regulated kinase (ERK 1/2) but didn't inhibit c-Jun N-terminal kinase (JNK) and p38, indicating that OMSDE may inhibit the pro-inflammatory cytokine production process by inhibiting the activation of $NF-{\kappa}B$ and ERK 1/2. Furthermore, OMSDE inhibited the production of interferon $(IFN)-{\beta}$ but didn't inhibit of $IFN-{\alpha}$ in the LPS-stimulated macrophages through the down-regulation of interferon regulatory factor (IRF)-1 and IRF-7. The Oral administration of OMSDE inhibited LPS-induced endotoxin shock and the production of $TNF-{\alpha}$ in serum but didn't inhibit of $IL-1{\beta}$ and IL-6. Conclusion: These results suggest that OMSDE may be effective in the prevention and treatment of inflammatory diseases.