• Journal of Korean Ophthalmic Optics Society
• /
• v.5 no.2
• /
• pp.33-42
• /
• 2000

This study was conducted for 356 customers coming to N optometrist office in Masan city in order to evaluate practice of hygienic control of contact lens storage cases. The results were as follows: 1) For disinfection of contact lens storage case, students used wetting saline solution(42.6%), exclusive wetting solution(23.0%) and 21(11.5%) of them didn't practice disinfection. Others besides students used wetting saline solution(29.1%), boiling water(27.3%) and 42(25.5%) of them didn't practice disinfection(P<0.01). 2) For storage case washing solution, 18(85.7%) out of 21 people who belong to high economic class used wetting saline solution, 3(14.3%) used exclusive wetting solution, 141(48.0%) out of 294 people who belong to middle economic class used wetting saline solution, 129(43.9%) used exclusive solution(P<0.01). 3) For wetting solution of storage case, 132(74.6%) out of 177 students used exclusive wetting solution and the rest(25.4%) used wetting saline solution, 102(64.2%) out of 159 others besides students used exclusive wetting solution, 51(32.1%) used wetting saline solution and 6(3.8%) used sold water and city water(P<0.05). 4) 66(94.3%) out of 70 contact lens storage cases were contaminated by bacteria and Serratia marcescens was isolated in 33(47.1%) out of 70 contact lens storage cases.

• Journal of Korean Ophthalmic Optics Society
• /
• v.4 no.2
• /
• pp.81-86
• /
• 1999

Human corneal epithelial cells and mouse fibroblast L929 cells were grown to 60-70% confluency in 96 well plates. 24 hours after the well plates are inoculated, the medium on the test plates is aspirated and replaced with an extract supplemented medium prepared from the materials to be tested. The contact lenses manufactured by 7 companies were collected from optical shops and used for this study. The exactracts having been prepared by autoclaving 8 lenses in 2.5ml saline. The cell monolayer is then cultured for a further 48 hour period. MTT and SRB assys were performed for cytotoxic effect on cultured cells An inhibition of 30% is considered clear indication of cytotoxic potential in the test material. All the materials were not cytotoxic, but 3 storage solutions of them inhibited growing L929 cells.

• 대한생식의학회:학술대회논문집
• /
• 1997.11a
• /
• pp.76-77
• /
• 1997

• Journal of Embryo Transfer
• /
• v.32 no.3
• /
• pp.235-241
• /
• 2017

To preserve genetic materials, cryopreservation of the semen from live animals is the main technique to establish cryo-banking system which could be used for artificial insemination and embryo transfer. However, the population of Korean black goat (KBG) becomes to dwindle in number and is now faced genetic erosion by crossbreeding with non-native breeds in small KBG farms. In this study, simple freezing method was used to preserve frozen semen from KBG using spermatozoa of cauda epididymis (CE) and electro-stimulated semen (ES). The negative effects of seminal plasma on fresh sperm was confirmed using precipitation test of Triladyl egg yolk diluent and sperm viability after thawing was compared between CE and ES spermatozoa. When seminal plasma of fresh ES semen was washed with semen washing media (SWM), the rates of live sperm shown no significant difference between CE and ES spermatozoa before freezing. However, the survival rate of frozen/thawed CE sperm was higher than ES ($74.6{\pm}10.6%$ vs $53.8{\pm}5.2%$) with significant difference (p < 0.05). The results of longevity test on frozen/thawed sperm from CE showed healthier sperm than ES. Therefore, spermatozoa from CE could be used for cryo-banking system in KBG lines. The more studies are needed to increase survival rate of ES semen.

• Journal of Korean Society of Rural Planning
• /
• v.26 no.1
• /
• pp.1-12
• /
• 2020

다수의 국가에서 농촌이 쇠퇴하는 현상을 확인할 수 있으며, 특히 경작 포기지의 비율이 빠른 속도로 향상되고 있다. 일본에서 경작 포기지의 증가는 농작물의 국내 자급률 감소, 농지의 중요한 기능 중 하나인 자연재해 방지 기능의 상실, 그리고 농촌 지역사회가 계승해 오던 다양한 형태의 무형적 자산의 소실 등을 야기하였다. 농지와 농촌 지역사회 활동의 보존은 지속가능한 개발을 위해 필수적인 요소이다. 생태계 서비스는 농지가 보유한 기능 중 하나이며, 일본의 중산간 지역 직불제는 농지의 보전과 농촌 지역사회 활동을 지원하여 생태계 서비스 보존 및 유지에 일조하기에 생태계 서비스에 대한 지불(PES)이라 볼 수 있다. 본 연구의 목표는 중산간지역 직접지불제도의 혜택을 받는 지역과 RCP8.5 시나리오 하에서의 자연재해 피해액 간의 관계를 파악하여 직불제의 효율성을 간접적으로 검증하는 것이다. 본 연구의 대상지는 일본 홋카이도의 농지 전체이며, 2005년, 2010년, 2015년의 강우량 예측 패널 데이터와 농업 센서스 패널 데이터를 이용하여 한계효과를 구하여 분석하였다. 분석 결과, 중산간지역 직불제 해당지역이면서 농촌 지역사회 활동이 활발할수록 자연재해 피해액이 적었다. 따라서 특히 중산간지역 직불제 해당지역일수록 재해피해 감소를 위해 농촌 지역사회 활동이 필수적으로 요구된다. 본 연구의 의의는 중산간지역 직불제의 효율성을 자연재해 피해액을 통해 검증한 데 있으며, 향후 직불제의 효율성에 대한 논의에 기초자료로 활용될 수 있다.

• The Korean Journal of Food And Nutrition
• /
• v.16 no.1
• /
• pp.29-34
• /
• 2003

Prunus mume has been used for the folk medicine by many old civilizations to treat food-borne diseases or enteric disorders. This study was carried out to examine the antimicrobial effect of juice from Prunus mume against pathogens such as Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. The juice of Prunus mume had the strongest antimicrobial activity to Sal. enteritidis. The concentrations of Prunus mume juice for the formation of clear zone were 1% for Sai. enteritidis(15.0mm), 3% for Lis. monocytogenes(14.7mm), and 5% for Bac. cereus(14.75mm), Esc. coli(13.45mm) and Sta. aureux(11.9mm). The growth of all tested microorganisms was inhibited apparently in tryptic soy broth containing 3% and 4% Prunus mume juice. And it was found that the Prunus mume juice showed the highest antimicrobial properties, followed by Sal. enteritidis, Bac. cereus, Sta. aureus, Lis. monocytogenes, Esc. coli.

• 대한생식의학회:학술대회논문집
• /
• 2004.06a
• /
• pp.87-87
• /
• 2004

• Korean Journal of Poultry Science
• /
• v.48 no.4
• /
• pp.185-191
• /
• 2021

Chicken spermatozoa have the ability to survive in low-temperature environments; however, the effects of low temperature on sperm motility and acrosome damage have not been studied in detail. The present study investigated semen longevity following dilution of rooster semen with Beltsville Poultry Semen Extender (BPSE) and Lake extender in preservation vessels (1.5 mL e-tube and 0.5 mL straw). Spermatozoa motility in the closed-type vessel (0.5 mL straw) was higher than that in the 1.5 mL e-tube on day 3 of preservation (68.6±3.1% vs. 22.1±5.7%). The motility of rooster semen diluted with BPSE in 0.5 mL straw was also higher than that of the Lake extender on day 3 of preservation (57.7±5.6% vs. 37.7±5.4%). Furthermore, acrosome intactness was higher in 0.5 mL straw than in the 1.5 mL e-tube, and the rate of acrosome cap damage increased with preservation days. The present study demonstrates that a closed 0.5-mL straw vessel could be used for low-temperature semen preservation, with an increased motility rate and acrosome integrity in fresh rooster semen.

• Journal of Aquaculture
• /
• v.10 no.3
• /
• pp.273-279
• /
• 1997

Condition for fresh storage of tiger puffer in the liquid state were investigated in several experiments. When marine fish ringer solution and 1% NaCl were used as the diluent for the short-term preservation method, spermatozoa activity index (SAI) and survival rate showed the best result among the various diluents tested. The dilution rate for the shortterm preservation of spermatozoa was suitable between 3 and 5 times with the 1% NaCi diluent. The appropriate range of temperature for the short-term preservation showed between 0 and $5^{\circ}C$. In order to keep high SAI and survival rate of spermatozoa, antibiotic addition (800 ppm neomycin) could be suggested. These results indicated that the short-term preservation method could be employed in tiger puffer spermatozoa.

• KSBB Journal
• /
• v.30 no.5
• /
• pp.253-256
• /
• 2015

The antibacterial activity from Tricholoma matsutake extracts were confirmed by minimum inhibitory concentration (MIC) test against various bacteria. Tricholoma matsutake extracts was applied for manufacturing low salted Jeot-gal with sodium DL-malate and sodium nitrite as food preservatives. Due to antibacterial activity from Tricholoma matsutake extracts, MIC of sodium DL-malate and sodium nitrite were significantly reduced to 0.025 g/L and 0.25 g/L, respectively. As a result of this study, a premium low salted Jeot-gal can be developed with low concentration of food preservatives by adding Tricholoma matsutake extracts.