• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.584-590
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• 2011

Wound inducible P450-Esg cDNA, one of cytochrome P450 gene family, was isolated from shoot of Euiseong garlic cultivar. P450-Esg cDNA possesses highly conserved heme-binding domain in the nucleotide sequence, and 1,419 bp of open reading frame (ORF) coding of 473 amino acids. Based on the nucleotide sequence analysis of P450-Esg homologous from twelve garlic cultivars, two domains, one domain between 472 to 510 bp, and the other between 1,210 to 1,249 bp from start codon (ATG), showed various nucleotide polymorphism among cultivars. Sequence of heme-binding domain in P450-Esg homologous, which is located at the domain between 1,210 to 1,240 bp from start codon, showed various nucleotide polymorphism as well as amino acid sequence polymorphism among twelve garlic cultivars. Anther domain, between 472 to 510 bp from start codon, showed exactly same amino acid sequence in the twelve garlic cultivars, but there were various single nucleotide polymorphism to the cultivars.

• Journal of ILASS-Korea
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• v.9 no.2
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• pp.1-8
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• 2004

This work was performed to investigate the distribution of the fuel droplet size around the bluff-body and the combustion characteristics. The geometry of the bluff-body influenced the spray shape and the combustion characteristics. Diameters of the bluff-body in this experiment are 6, 8, and 10 mm and the impingement $angles({\theta})\;are\;30^{\circ},\;60^{\circ},\;and\;90^{\circ}$. The measurement points were at the distances of 20 and 30 mm axially from the nozzle. The SMD and Rosin-Rammler distribution was acquired by image processing technique (PMAS), and the mean temperatures were measured by thermocouple. The results obtained are as follows; In the condition of ${\theta}=60^{\circ}$, the values of SMD are not greatly varied compared to the other conditions. As the impingement angle of bluff-body was increased, the high temperature region was wider along radial direction. When the air-fuel ratio was increased, the CO concentration was decreased.

• Economic and Environmental Geology
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• v.30 no.5
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• pp.477-491
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• 1997

In the Bibong area of the western part of Chungcheongnam-do, ultramafic masses occur as discontinuous isolated lenticular bodies in the Precambrian Kyeonggi gneiss complex. They extend for about 200 m long to NNE directions which are parallel to fault lines in the gneiss complex. The ultramafic masses contact with the adjacent gneiss complex as steeply dipping faults. They are dunites and harzburgites and many of them are partially or completely serpentinized. The ultramafic rocks dominantly show protogranular, equigranular and equigranular-$m{\grave{o}}saic$ textures. They also show porphyroclastic (megacrystic) or recrystallized textures reflecting several stages of metamorphism. They contain varying amounts of olivine $(Fo_{89-92})$, enstatitic to bronzitic orthopyroxene, diopsidic clinopyroxene, tremolitic to pargasitic hornblende, and spinel with serpentine, talc, chlorite, calcite and magnetite. The ultramafic rocks have high magnesium numbers and transitional element contents, low alkali contents and show deplete REE patterns. Comparing with available data, geochemical and mineralogical characteristics shown in the ultramafic rocks of the Bibong area are similar to those of worldwide mantle xenoliths and orogenic related ultramafic rocks. The field evidences, petrographical, geochemical and mineralogical characteristics shown in the ultramafic rocks of the Bibong area are similar to alpine type ultramafic rocks emplaced into the crust by the faulting as mantle slab types. With the petrographical characteristics, these mineralogical compositions suggest that the ultramafic rocks of the Bibong area have experienced several stages of retrogressive metamorphism in a condition ranging from the upper amphibolite facies to greenschist facies.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.94-94
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• 2003

Rotavirus는 유아나 어린아이들에게 가장 일반적으로 나타나는 심한 위장염의 원인자이며 설사로 인한 심한 탈수 증세를 일으켜 급속히 성장하는 유아의 균형적인 영양 공급을 방해함으로써 유아들의 발육과 성장 그리고 심하면 생명에 커다란 영향을 미치게 된다. 한편 모유로 키운 유아들은 설사병의 낮은 발병율과 연관이 있었다. 특히 모유의 뮤신 복합체는 rotavirus에 특이적으로 결합하여 항 바이러스활동을 보여주는 것으로 나타났다. 이러한 배경에서 본 연구는 human breast tissue로부터 lactadherin의 cloning 및 sequence 분석을 통하여 유전자의 다양성을 조사하기로 하였다. 한국 여성 9명의 유두 근처 조직에서 lactadherin을 cloning하여 그 sequence를 보고 된 서양여성의 염기서열과 비교 분석결과 여러 곳에서 single nucleotide variation이 발견되었고 본 연구에서 클론한 lactadherin(31bp-1518bp)의 염기서열과 보고된 서양여성 lactadherin gene의 SNP와 비교하였을 때 8개의 SNP중 3부분만이 일치한다는 것을 확인하였다. 또한 같은 조직중 정상 조직과 암 조직 부분에서 각각 lactadherin을 클론하여 염기서열을 비교 분석하였는데 정상 조직에서 2곳의 silent mutation있었고 암조직에서 2곳의 mutation과 1곳의 silent mutation을 발견하였으며 전체 적으로 정상조직과 암 조직 부분에서 lactadherin을 clone하여 염기서열을 분석해본 결과 암조직일수록 유전자의 변이 비율이 높다는 것을 알 수 있었다. 그리고 동일한 염기서열 상에서 많은 변이가 일어났는데 286dp(A->C), 1418dp(G->C)은 mutation이었고 327dp (A->G), 454(C->T)은 silent mutation이었다. 그 외 DNA상에서 여러 부근에 변이가 존재하였는데 이 결과로 보아 coding region에 위치한 cSNP 중 amino acid 변화를 일으켜 protein structure 또는 function에 영향을 줄 수 있는 non-synonymous cSNP 일 것으로 예상되어지며 natural selection의 영향을 받고 있음을 암시하고 있다. 본 연구에서 관찰되어진 각각의 염기 서열의 변이는 한국 사람이 가지는 lactadherin gene의 cSNP의 일부라고 판단하였다.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.67-68
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• 2003

한국 재래 닭의 유전적 특성 규명 및 기능 유전체 연구의 기초재료를 확보하고자 대량 EST 염기서열 결정 및 생물정보학 분석을 실시하였다. 대량 EST 염기서열 분석을 위한 첫 번째 단계로 재래 닭의 뇌, 비장, 정소, 배아 생식기를 이용하여 cDNA library를 구축하였다. 각각의 library로부터 총 15,121개의 클론을 선정하여 염기서열을 결정하였다. 생물정보학 분석결과 15,121개의 염기서열은 총 10,353개의 contig로 정리되었다. 이들 염기서열을 기존 데이터베이스를 대상으로 tBlastX(http://www.ncbi.nlm.nih.gov/BLAST) 분석을 실시한 결과, 염기서열 중 56 ％가 기존 데이터베이스에 존재하는 유전자와의 상동성을 보였다. 상동성을 보이는 유전자들은 유전자의 구조 및 기능 분석에 이용될 것이고, 상동성을 보이지 않는 유전자들은 microarray와 같은 대량 유전자 발현분석 시스템을 이용하여 선별한 후 기능분석이 실시될 것이다.

• Korean Journal of Microbiology
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• v.36 no.4
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• pp.267-272
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• 2000

A 1.7-kb fragment containing the nodD1 genes of Bradyrhizobium sp. (Cassia) CN9135 was amplified by PCR with primers based on B. japonicum USDA110. This fragment was cloned and sequenced. Analysis of the sequence showed open reading frames highly homologous to nodD1 from other bradyrhizobial sources. The sequence showed higher homology to nodD1 gene of B. elkanii than to those from b. japonicum. Our results suggest that Bradyrhizobium sp. (Cassia) CN9135 may be more closely related to B. elkanii than to B. japonicum.

• Animal Systematics, Evolution and Diversity
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• no.nspc3
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• pp.139-146
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• 1992

The primary sequence of the 18S rRNA gene of a crustacean Pugettia quadridens (Decapoda: Pleocyemata: Brachyura) was determined by the PCR cloning and Taq sequencing. The 18S rRNA gene of this species in 1837 bases long, and 46 bases shorter than that of another crustacean decapod Oedignathus inermis. The similarity between two species is 90.8% when the insertion and/or deletion sites were excluded. Within the molecule, the most conservative (identical) region locates at the position of 1137-1206 and it is 70 bases long. The most long consecutive nucleotide differences occur at the position between 46-55 and the second most between 399-407. The sequence variation in the primary structure of 18S rRNA gene are not evenly distributed throughout the molecule.

• Journal of the Korean Society of Combustion
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• v.3 no.1
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• pp.1-10
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• 1998

Lots of techniques are adopted for a flame stabilization and a high-load combustion. But the techniques being used were passive control method which have to change combustor shape like pilot flame, flame stabilizer, pressure profile, etc. Active control method which is not necessary to transform its shape is employed. Acoustical excitation is broadly used for its convenience in changing frequency and intensity. Both acoustical excitation and flame stabilizers were adopted to study their relationship. So, we investigated flammability limits. Flame visualization. And mean temperature in the condition of various frequencies, intensities, and flame stabilizers. As a consequence, flammability limit were advanced in acoustically excited flame at some frequencies. Coherent structure was extended to the downstream region through acoustical excitation and a size of vortice was curtailed. Also width of recirculation zone was magnified. In addition, Effects of acoustical excitation was stood out at 25mm flame stabilizer rather than another ones.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.05a
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• pp.328-331
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• 2008

In order to see the detailed characteristics of model scramjet engine, numerical analysis was performed and compared to the ground test results done by KARI and UQ. Pressure distribution predicted by numerical analysis showed good agreements with test results. Static temperature and pressure distribution explained the mechanisms of cavity flame holder and W-shape cowl which have showed enhancing effects on the supersonic combustion.

• Transactions of the Korean Society of Mechanical Engineers
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• v.9 no.4
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• pp.497-508
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• 1985

본 논문에서는 일양한 예혼합 기류중에 놓여진 원주 후류의 고온 순환류에 의 해 보지되는 난류 예혼합 화염을 대상으로 해서 유동의 가시화 및 온도와 이온전류의 변동의 측정에 의해서 화염의 구조를 조사한 결과, 원주 보염기 후류의 재순환 영역부 근에 형성된 전단층에 있어서는 코히렌트(Coherent)구조의 화염이 되고 하류부에서는 불규칙한 3차원 와(渦)에 지배되는 전파성 화염이 형성 되었다. 온도변동에 대한 쌍 봉성의 확율밀도분포와 이온전류변동에 대한 3개의 피이크의 확율밀도분포는 엷은 반 응면을 사이에 두고 미연혼합기괴와 기연가스괴가 서로 접하는 주름 상층류화염 또는 층류화염편의 구조에 대응하며, 코히렌트 와(渦)에 지배되는 화염에 있어서는 거시적 혼합은 코히렌트 와의 거동에 지배되나 그 구조는 주름상층류 화염과 일부 강한 전단 력을 받는 부분에는 분산 반응영역의 구조임이 밝혀졌다.