• Title/Summary/Keyword: 변이음

Search Result 14,784, Processing Time 0.052 seconds

Protease Activity of Lactic Acid Bacteria Isolated from Korean Traditional Fermented Food (전통 발효식품으로부터 Protease 활성을 보유한 유산균의 분리 및 동정)

  • Kook, Moo Chang;Cho, Seok Cheol;Park, Hoon;Kim, Seung Seop;Pyun, Yu Ryang;Choi, Woon Yong;Lee, Hyeon Yong
    • Food Engineering Progress
    • /
    • v.15 no.2
    • /
    • pp.182-187
    • /
    • 2011
  • A proteolytic lactic acid bacterium was isolated from Korean traditional fermented foods. The isolate BV-26, which had a protease activity (24 U/mg-crude protein), was identified as Lactobacillus plantarum by the API 50CHL kit and 16S rDNA analysis (99.9% of homology), and named as L. plantarum BV-26. Cell growth and protease activity of L. plantarum BV-26 was determined in MRS broth using 5L jar fermentor at $30^{\circ}C$. The maximum growth of L. plantarum BV-26 was reached at 18 hr in MRS broth, while protease activity of BV-26 was detectable at 12 hr and the highest activity was obtained after 16 hr cultivation. Therefore, we expect that the proteolytic lactic acid bacteria, L. plantarum BV-26, may be used as a starter for the fermentation of animal feed. Especially, the fermentation of soybean meal with the strain can be applied for improving feed utilization.

Analysis of Quantitative Trait Loci (QTL) Associated with the Gel Consistency in Rice (쌀의 호응집성에 대한 QTLs 분석)

  • Kim, Tae-Heon;Sohn, Jae-Keun;Kim, Kyung-Min
    • Korean Journal of Breeding Science
    • /
    • v.41 no.4
    • /
    • pp.474-481
    • /
    • 2009
  • The purpose of this study was to identify the quantitative trait loci (QTL) associated with gel consistency (GC), and to evaluate the relationships between GC and physico-chemical properties related to eating quality in rice. A total of 120 doubled haploid (DH) lines derived from a cross between 'Samgang' and 'Nagdong' and 26 varieties were used for this study. The 120 DH lines were divided into four groups, soft (61~100 mm), medium (41~60 mm), hard (26~40 mm) and whole population, according to the gel length. A negative correlation between GC and amylose content was observed only in the soft group. The GC negatively correlated with lipid content in the medium, and the whole population, but positively correlated with lipid content in the soft group. The positive correlation between GC and protein content was observed in the soft group, while negative correlations were observed in the medium group and the whole population. Two significant QTLs regarding GC were detected on chromosomes 4 and 11. They collectively explained 23% of phenotypic variation with LOD score of 3.1 and 2.9, respectively. The DNA markers of S4026 and RM287 were tightly linked to GC in the DH population and 26 varieties, respectively.

Analysis of Bus Drivers' Working Environment and Accidents by Route-Bus Categories : Using Digital TachoGraph Data (노선버스 운송업종별 운전자의 근로여건 및 사고 분석 : DTG 데이터를 활용하여)

  • Kwon, Yeongmin;Yeo, Jiho;Byun, Jihye
    • The Journal of The Korea Institute of Intelligent Transport Systems
    • /
    • v.18 no.2
    • /
    • pp.1-11
    • /
    • 2019
  • The accident of mass transit such as a bus could draw the large casualties and this induces social and economic losses. Recently, severe bus accidents caused by tiredness and inattention of bus drivers occurred and those lead to growing interest in bus accidents and the drivers' work environment. Therefore, this study analyzes the accident based on the work environment of bus drivers and route-bus categories. For the research, this study collected digital tachograph data and the bus company information for 271 domestic bus companies in 2017 and used ANOVA test and chi-square test as statistical methodologies. As a result, we figured out there are statistically significant differences in the accident according to the working environments. Especially, the present study confirmed the intracity bus with working every other day has the most frequent accidents. We expect that the results of this study be used as foundations for the improvement of working conditions to reduce route-bus accidents in the future.

Measurement of and Changes in L-carnitine Levels in Developing Cucumber Cotyledon (오이 떡잎의 발달 과정에서 carnitine의 검출과 변화)

  • Cha, Hyeon Jeong;Kim, Dae-Jae
    • Journal of Life Science
    • /
    • v.29 no.4
    • /
    • pp.421-427
    • /
    • 2019
  • Mobilization of storage lipids is critical for the germination of oil seeds, as they supply carbon and energy until photosynthesis commences in cotyledons. In this study, we determined the levels of plant carnitine and associated changes in these levels from seed germination to cotyledon senescence. We also examined changes in the content of unsaturated fatty acids throughout seedling development. Carnitine levels peaked on day 3 at 14.5 nM in cotyledons and decreased sharply to 7.2 nM on day 4. On development day 3 carnitine levels were maintained at around 3 nM until day 7. The unsaturated fatty acid content dropped by half at the same time as carnitine peaked (day-3), and storage lipids were almost depleted by day 5. Thereafter, carnitine was hardly detected until the second stage of cotyledon senescence, at which stage the carnitine content was 6.8 nM, similar to that on day 4 at the time of fatty acid depletion in the cotyledons. Unsaturated fatty acids levels remained constant in green cotyledons but slightly increased in the senescing cotyledons. The latter can be explained by intracellular breakdown of membrane lipids. This is the first such discovery in developing cotyledons and may offer clues regarding other roles of the acetyl unit transport system in plants. The expression of BOU was closely associated with carnitine metabolism during seed germination and cotyledon development. The results provide support for the possibility of carbon re-routing during the glyoxylate cycle in the supply of energy for early germination and development.

Analysis of Relevant Factors and External Quality Assessment in Small-Medium Sized Hospital (중소의료기관의 외부정도관리 시행과 관련요인 분석)

  • Kim, Hye-Jeong
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.20 no.2
    • /
    • pp.294-299
    • /
    • 2019
  • Korean hospitals implement external quality assessment (EQA) schemes to improve the quality of clinical tests. However, smaller hospital do not implement EQA due to lack of appreciation and cost burdens. The purpose of this study was to analyze the results of EQA from the perspective of clinical chemistry carried out at a small-to-medium sized hospital (SMH) and to investigate factors influencing quality control. The medical institution concerned had performed EQA on 21 items annually for three years and had analyzed measurement methods, variation coefficients, and anomalous results as defined by the SMH. No significant change in test methods or variation coefficients, which exhibited a high level of variation, were observed for any test item over the 3-year period. The results obtained showed that anomalous test results were significantly more frequent when EQA was not conducted (p<0.05), internal quality control was not conducted daily (p<0.01), and when tests were less frequently performed (p<0.01). Small-to-medium sized hospitals need to be more aware of the benefits of EQA and provide tailored education to staff.

Study on Stable Gait Generation of Quadruped Walking Robot Using Minimum-Jerk Trajectory and Body X-axis Sway (최소저크궤적과 X축-스웨이를 이용한 4족 보행로봇의 안정적 걸음새 연구)

  • Lee, Dong-Goo;Shin, Wu-Hyeon;Kim, Tae-Jung;Lee, Jeong-Ho;Lee, Young-Seok;Hwang, Heon;Choi, Sun
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
    • /
    • v.12 no.2
    • /
    • pp.170-177
    • /
    • 2019
  • In this paper, three theories for improving the stability of quadruped robot are presented. First, the Minimum-Jerk Trajectory is used to optimize the leg trajectory. Second, we compare the newly proposed sine wave and the conventional LSM in this paper based on the Jerk value. Third, we calculate the optimum stride of the sway through repetitive robot simulation using ADAMS-MATLAB cosimulation. Through the above process, the improvement of the robot walking is compared with the existing theory. First, the average gradient of the point where the leg trajectory changes rapidly was reduced from at least 1.2 to 2.9 by using the Minimum-Jerk targetory for the movement of the body and the end of the leg during the first walk, thereby increasing the walking stability. Second, the average Jerk was reduced by 0.019 on the Z-axis, 0.457 on the X-axis, and 0.02, 3D on the Y-axis by 0.479 using the Sin wave type sways presented in this paper, rather than the LSM(Longitude Stability Margin) method. Third, the length of the optimal stride for walking at least the Jerk value was derived from the above analysis, and the 20cm width length was the most stable.

The Recognition of Special Teachers for Early Childhood about the Guarantee of Rights of Infants and Toddlers with Disabilities (유아특수교사의 장애영유아 권리보장에 대한 인식)

  • Kim, Sam-Sup
    • Journal of Digital Convergence
    • /
    • v.17 no.2
    • /
    • pp.475-487
    • /
    • 2019
  • The purpose of this study was to investigate whether there are any differences in the recognition of the level of the guarantee of the rights of infants and toddlers according to the teachers' variables(gender, age, working experience) and institutional variables (institution type, establishment type, scale, area). We surveyed 365 special education teachers and found out differences. The results are as follows. First, there was no difference in the recognition according to gender. Second, there was no difference in the recognition according to age. Third, there was no difference in the recognition by working experience. Fourth, the recognition by type of educational institution showed that there were differences in the ambit of rights to life, protection, development, and participation. Fifth, the recognition according to the establishment type showed there were differences in the rights to life and development. On the other hand, there was no difference in the rights to protection and participation. Sixth, there were differences in the recognition about the rights to life, protection, development, and participation according to the scale of the education institutions. Seventh, there were no differences in the region. The results of this study can be used as basic data for establishing policy for the promotion of educational rights for infants with disabilities.

Effect of Fire Safety Education Based on the Theory of Planned Behavior on the Fire Safety Behavior of Care Worker Trainees (계획된 행동 이론을 적용한 화재안전교육이 요양보호사 교육생들의 화재안전행동에 미치는 효과)

  • Byeon, Do-Hwa
    • Fire Science and Engineering
    • /
    • v.33 no.1
    • /
    • pp.147-155
    • /
    • 2019
  • This study was a quasi-experimental research of a non-equivalent control group and non-synchronized design for analyzing the effects of fire safety education on care worker trainees. The subjects of the study were care worker trainees of the S Care Worker Institution: 57 trainees with 28 in the experimental group, and 29 in the control group. The research period was from May 21 to June 14, 2018 and the experiments focusing on fire safety education were performed once per week for a total of four times over the research period. The data were then analyzed using a ${\chi}^2-test$ and t-test. The results showed that fire safety education is an effective source of education for increasing the knowledge on fire safety, attitudes towards fire safety, perceived behavioral control on fire safety, behavioral intention on fire safety, and fire safety behavior in addition to being incredibly useful in practicing fire safety behaviors throughout their daily lives. On the other hand, the subjective norms on fire safety did not show any significant differences. Therefore, this paper suggests a follow-up study that should focus on analyzing the effects of the subjective norms on fire safety.

Seasonal Distribution of Mosquitoes According to Habitat Environment (2016-2018) (서식환경에 따른 모기 분포조사(2016-2018))

  • Park, Geun-Ho;Kim, Sung Il;Cho, Sung Woo;Cho, Sun-Ran;Lee, Seung-Ju;Kim, Hyun Kyung;Koo, Hyun-Na;Lee, Wook-Gyo;Cho, Shin-Hyeong;Kim, Gil-Hah
    • Korean journal of applied entomology
    • /
    • v.57 no.4
    • /
    • pp.381-392
    • /
    • 2018
  • The distribution of mosquitoes collected in the Cheongju area from 2016-2018 were compared. The collection sites were established at a residential area, migratory bird sanctuary, and cowshed using black light (BL) and BG sentinel traps. Culex pipiens was the most collected species in the residential area, and Aedes vexans was the most collected species in the migratory bird sanctuary and cowshed in all years. The BG trap collected more individuals than did the BL trap in the residential area and migratory bird sanctuary. In total, 22,679 (10 species) mosquitoes were collected in 2016, and 6,502 (8 species) and 6,803 (9 species) mosquitoes were collected in 2017 and 2018, accounting for 3.49- and 3.33-fold decreases, respectively. The relationship between meteorological conditions and mosquito density, according to various variables, was not significant different. The Chaoyang virus was found in Aedes vexans and Culex pipiens collected at the cowshed in 2016 and 2018, respectively. Based on the results of this study, it is considered that continuous surveillance of mosquitoes should be performed for controlling mosquito populations and mediating diseases spread by this vector.

Influence of Core Competencies of Lifelong Educators on the Lifetime Ability of Adult Learners: Focusing on Mediating Effect of Adult Learners' Wisdom (평생교육자의 핵심역량이 성인학습자의 생애능력에 미치는 영향: 성인학습자 지혜의 매개효과를 중심으로)

  • Kim, Eun-Im;Song, Sun-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.19 no.11
    • /
    • pp.711-720
    • /
    • 2018
  • The purpose of this study was to explore the impact of lifelong educators' core competencies as a mediating effect on adult learners' ability to function. The subject of this study was aimed at adult learners participating in lifelong education programs at lifelong educational institutions located in Gyeonggi Province. and was finally used for a total of 520 additional analyses. The main findings of this study are as follows: first, regarding the correlation between the core competence of lifelong educators and the life ability of adult learners, core competence has a static association with wisdom. In addition, wisdom has a high static association with life expectancy. Second, the core competence and wisdom of lifelong educators were found to have a significant static impact on adult learners' life abilities, accounting for 66.8%. Specifically, the most significant predictors of life's ability were creative problem solving, consideration for others, and acceptance of change, which were lower factors of wisdom. Third, the impact of a lifetime educator's core competence on adult learners is not significant. It was found that core competence has an indirect influence on life capacity through the mediating effect of wisdom, and that wisdom has directly interfaced the relationship between core competence and life ability. Taken together, these results proved that the core competence and wisdom of a lifelong educator are important variables for adult learner's life abilities. Based on this, the core competence of lifelong educators being taught in the field of lifelong education should be enhanced, and the ability of adult learners to utilize the wisdom that has been developed through the experiences of adult learners, to improve their life skills.