• The Korean Journal of Pharmacology
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• v.8 no.1
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• pp.15-25
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• 1972

Korean aconitum (Aconitum koreanum R. Raymond) as one of the botanical crude drugs which pertain to helleboraceae has been extensively applied in Chinese medicine during the past decades. It has been particularly used in immortal tonic among the folk remedies in China, however, its general uses comprehend diuresis, cardiotonic, analgesia, neuralgia, gout and, furthermore, even neoplastic effect. The components of aconitum have been acknowledged as aconitine, mesaconitine, hypaconitine, aconine and so on. The main ingredient, aconitine has the advantage of causing the atrial fibrillation, but, its pharmacological research has not been fully elucidated. Although there are many reports with regard to the pharmacological effects on the motility of several animal hearts, their conclusions have not been regretfully coincided yet. The authors hereby paid attention to this point of view and made experiment to examine the relationship between the alcohol extract of Korean aconitum and the motility of the isolated clam heart, making the use of several drugs related to the heart such as serotonin, acetylcholine, pilocarpine, physostigmine, barium chloride, procaine and quinidine. The cardiac movement of the isolated clam (Meretrix lusoria) heart in the standard sea water solution was recorded with the electric kymograph according to the Magnus method. The results of the experiment are as follows. 1. The motility of the isolated clam heart represents the tendency of gradual inhibition in proportion to the concentration of AK-A $10^{-4}$, $5{\times}10^{-4}$, and $10^{-3}$. 2. The cardiac inhibitory effect of AK-A $10^{-3}$ antagonizes the motility of the isolated clam heart pretreated with serotonin $10^{-6}$. 3. The cardiac inhibitory effect of AK-A $10^{-3}$ antagonizes the systolic state appealed by barium chloride $10^{-3}$. 4. The systolic state caused by quinidine $10^{-4}$ is not inhibited by AK-A $10^{-3}$.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.2
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• pp.346-349
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• 2008

To investigate whether aqueous extract from Aconitum koreanum (AEAK) effects in the process of melanin synthesis, the expression of tyrosinase-related proteins (TRPs) by immunohistochemical methods were performed in ultraviolet B (UVB) irradiated skin of guinea pig. The irradiation of UVB (60 mJ/day) was performed for 3 days and treated with AEAK for 15 days. About the color evaluation, the visual scores of UV B irradiated guinea pig with AEAK treatment were slightly lower than those in the UV B alone irradiated ones. At day 15 after UVB exposure, immunohistochemical analysis for TRPs expression were performed. The intensive expression of tyrosinase was mainly observed over epidermis with skin appendage and in the cells of dermis. Slight increase of these reaction was induced in response to UVB in the spinous and granular layer of epidermis, but similar expression in the AEAK treated guinea pig as normal one. The TRP-1 and TRP-2 expression were not detected in the skin of normal guinea pig. But intensive expression for TRP-1 and TRP-2, especially TRP-2, induced by UV B irradiation in the cells of dermis. These expressions were decreased in the AEAK treated guniea pig. Collectively, these results suggest that AEAK has a potential to inhibit synthesis through regulation of TRPs expression in the skin of guinea pig, but better understanding the function of AEAK, more research should be done in the effects of AEAK on the function of TRPs in melanogesis.

• The Korea Journal of Herbology
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• v.32 no.4
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• pp.33-38
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• 2017

Objectives : Termitomyces albuminosus (Berk.) Heim is one of the famous wild edible mushrooms in the southern part of China. It is known that Termitomyces albuminosus, like Aconitum koreanum used in Korean traditional medicine, contains a kind of cerebroside, termitomycesphin, causing a pharmacologic effect on the neuron system. The pharmacologic effect of Termitomyces albuminosus can be used to possibly replace Aconitum koreanum. However, It needs to be certified as safe before it can be used. Here, a single-oral toxicity test and safety classification was conducted to obtain acute information of the toxicity of dried-Termitomyces albuminosus powder and to secure its safety in clinical applications. Methods : In order to calculate approximate lethal dose(ALD), test substance was orally administered to male and female SD-rat at dose levels of 5,000 and 0 (vehicle control) mg/kg (body weight). Based on the result of this toxicity, also the estimation of safety classification was calculated using the HED-based (human equivalent dose) MOS (margin of safety). Results : There were no mortalities, test substances treatment-related clinical signs, no changes in the body or organ weights, and no gross or histopathological findings at 14 days after treatment with test substance. Thus, the approximate lethal dose of dried-Termitomyces albuminosus powder was considered over 5,000 mg/kg in both female and male mice. Conclusions : Based on the limit dose, 5000 mg/kg, it was estimated that dried-Termitomyces albuminosus powder is classified as "Specified class B" indicating that clinical dose is not limited to patients as safe as food.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.58-58
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• 2022

본 연구는 국내 6곳의 풍혈의 기능과 식물분포를 통하여 풍혈의 보전대책과 관리방안을 위한 기초자료를 확보하기 위해 진행하였다. 조사는 홍천 방내리, 정선 여탄리, 정선 운치리, 연천 동막리, 제천 금수산, 보은 구병산에 위치하는 풍혈 6곳을 선정하였고 풍혈에 기상측정장비를 설치하여 2021년 3월부터 2022년 3월까지 온도와 습도의 미기상요인을 측정하였다. 풍혈이 위치하는 지역의 미기상요인은 기상청 자료를 활용하였고 측정된 풍혈의 미기상요인과 비교 분석하였으며, 관속식물상은 계절별로 진행하여 각 풍혈에 서식하는 희귀식물과 북방계식물, 기후변화 취약식물을 분석하였다. 풍혈 6곳의 미기상요인 분석결과 온도는 보은 구병산 풍혈을 제외한 5곳의 풍혈은 여름철 냉혈의 기능을 나타냈고, 보은 구병산 풍혈은 겨울철 온혈의 기능을 하는 것으로 나타났으며, 습도는 6곳 모두 주변 지역보다 높게 나타났다. 멸종위기종 2급은 백부자, 산작약, 연잎꿩의다리 3분류군, 희귀식물은 산개나리, 월귤 등 23분류군, 북방계식물은 과남풀, 꽃개회나무 등 129분류군, 기후변화취약식물은 검종덩굴, 도깨비부채 등 23분류군으로 확인되었다. 풍혈은 기후변화 취약식물의 피난처로서 역할을 하는 것으로 나타났으나 산림유전자원보호구역으로 지정된 홍천 방내리 풍혈을 제외한 나머지 풍혈은 관리가 미흡하다. 정선 여탄리 풍혈은 인근 도로에서의 외래식물의 유입, 정선 운치리 풍혈은 인근의 양봉장과 관광객 등의 인간활동에 의한 훼손, 연천 동막리 풍혈은 인근 경작지 등의 사유지로 인한 관리의 어려움, 제천 금수산 풍혈은 관광지화로 인한 훼손, 보은 구병산 풍혈은 등산객의 답압으로 인한 훼손이 진행되고 있는 것으로 나타났다. 이에 따라 풍혈과 희귀 및 기후변화 취약식물들의 정기적인 모니터링과 풍혈 주변의 팬스 설치 등의 적극적인 보전방안이 필요하다고 판단된다.

• Korean Journal of Environment and Ecology
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• v.16 no.1
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• pp.104-123
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• 2002

The flora survey on Chungnyeongsan and Seorisan was conducted 6 times from 1998 to 2000.817 taxa such as 112 families 415 genera 688 species 1 subspecies 116 varieties 12 forma were recorded. Dominant species in investigated areas was Quercus mongozica and some deciduous broad-leaved species, such as Quercus sp., Acer sp., Fraxinus sp., Carpinus sp., etc. were codominant species in these areas. Pinus koraiensis and Larix teptozepis plantations were also found in these areas. The 16 taxa of rare plants protected by Forestry Service were found in these areas and among them 10 taxa were new populations which had not been recorded in these areas. Discovery of Erunthis stellata, Aconitum koreanum, Paeonia japonica, Scopotia japonica and Acunthopanax senticosus populations indicate that these areas could be regarded as very important one in a biogeographical aspect. Endemic plants were listed 35 taxa and among them 22 taxa including Corydalis maculata, Ulmus macrocarpa, Scrophutaria horuiensis, Cirsium setidens, etc. were new one. Naturalized plants were listed 39 taxa and among them 30 taxa were new one. Lower naturalized index(NI) and higher urbanized index(UI) were recorded compared to those of neighbored areas. There were many kinds of threatening factors fur the plant resources in survey areas, so it is necessary to limit the number of visitors and to introduce the natural relaxation system.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.399-402
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• 2002

Angiotensin converting enzyme(ACE) belongs to the class of zinc protease and plays an important role in the regulation of blood pressure. In this experiment, we investigated the inhibitory activities of medicinal plant extracts on ACE. Fifty medicinal plants were selected and the extracts were prepared by refluxing with 70% methanol. Among the extracts, eleven medicinal plant extracts such as Sedum sarmentosum Bunge, Petasites japonicus(s.et z.) Max, Rubus coreanus, Morus bombycis Koidz, Acorus calamus var. angustatus, Glycyrhiza glabra, Equisetum hyemale, Portulaca oleracea L., Prunella vulgaris var. lilacina Nakai, Sorbus commixta Hedl, Allium thunbergii showed more than 50% inhibitory activities, and Paeonia suffruticosa Andr., lnula helenium, Acanthopanax senticosus Harms, Dendrobium moniliforme, Juglans mandshurica, Zizyphus jujuba, Leonurus artemisia, Aster scaber Thunb, Vitex rotundifolia, Platycodon grandiflorum, Prunus persica, Ligularia fischeri showed $40{\sim}49%$ inhibitory activities. Therefore these extracts which contain high ACE inhibitory activities may be useful as antihypertension agents and to the treatment of hypertension.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.26-26
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• 2018

본 연구는 DMZ 및 접경지역에서 생육하고 있는 자생식물에 대하여 조사된 연구자료와 국립수목원 DMZ자생식물원에서 자체 조사로 수행된 미조사지역 연구 등을 집대성한 DMZ 일원의 관속식물상 가운데 행정구역상 고성군, 인제군, 양구군, 화천군에 해당하는 동부지역 관속식물의 유용자원 현황에 대하여 기초자료를 제공하고자 하였다. 동부지역은 고성군의 석호들을 포함한 저층습지 및 해안지역과 설악산(1,708m), 점봉산(1,424m), 화악산(1,468m), 등의 고산지대, 대암산(1,304m) 정상부근에 위치한 고층습지 용늪(1,280m)까지 이르는 다양한 생활환경 특성을 포함하고 있다. DMZ일원의 관속식물상 157과 754속 2,047종 12아종 340변종 105품종 총 2,504분류군 중 40.2%인 111과 438속 873종 4아종 113변종 17품종 1,007분류군이 확인된 동부지역 유용식물의 조사 결과 식용식물 순채, 고려엉겅퀴, 병풍쌈 등 336분류군(33.3%), 사료용식물 산뽕나무, 고광나무, 새 등 334분류군(33.1%), 약용식물 주목, 백부자, 삼지구엽초 등 270분류군(26.8%), 관상용식물 제비동자꽃, 백작약, 만병초 등 118분류군(11.7%), 목재용식물 일본잎갈나무, 소나무, 오동나무 등 38분류군(3.7%), 염료용식물 밤나무, 매발톱나무, 갈매나무 등 26분류군(2.5%) 섬유용 식물 개모시풀, 쐐기풀, 참싸리 등 8분류군(0.7%), 산업용식물 굴참나무, 붉나무, 노박덩굴 3분류군(0.2%), 용도를 알지 못하는 식물 280분류군(27.8%)으로 나타났다.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.1
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• pp.27-32
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• 2015

Streptococcus mutans triggers dental caries establishment by two major factors. One is synthesis of organic acids which demineralize dental enamel and the other is synthesis of glucans which mediate the attachment of bacteria to the tooth surface. In the present study, we evaluated the effect of the ethanol extracts of Aconitum koreanum (A. koreanum ) on the growth and acid production of S. mutans. Ethanol extracts of the A. koreanum showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.016 mg/ml compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by 50%, 54% at the concentration of 0.063, 0.125 mg/ml. On the study of activation of glucosyltransferase which synthesizes water insoluble glucan form sucrose, the ethanol extract of A. koreanum showed remarkable inhibition over the concentration of 0.016, 0.031, 0.063 and 0.125 mg/ml (p<0.05). Especially on the concentration of 0.063, 0.125 mg/ml, the extracts suppressed the glucan synthesis by 100%. We analyzed the component of the extracts of A. koreanum. The results showed that the extract of A. koreanum had strong phenolic compound, glycosides and organic acids. These results suggest that A. koreanum may inhibit the caries-inducing properties of S. mutans, and which may be related with strong phenolic compound, glycosides and organic acids.

• Korean Journal of Plant Resources
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• v.35 no.4
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• pp.541-563
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• 2022

This study aimed to elucidate the distribution of vascular plants in Namhansanseong (Gwangju-si, Gyeonggi-do), Korea. The results of 30 field surveys from June 2019 to June 2020 identified 568 total taxa, representing 513 species, 10 subspecies, 40 varieties, 3 forms, and 2 hybrids in 335 genera and 107 families. Among them, 17 taxa were endemic plants to Korea, and one taxon were endangered plant and three taxa were red list plants. The floristic target plants amounted to 63 taxa, specifically one taxon of grade V, seven taxa of grade IV, 12 taxa of grade III, 14 taxa of grade II, and 29 taxa of grade I. And 66 taxa were northern lineage plants. Alien plants were recorded a total of 61 taxa with a Naturalized Index of 10.7%, the Urbanization Index was calculated to be 9.8%. As the result of comparison with previous studies, in this study a total of 213 taxa were newly identified representing 187 species, three subspecies, 19 varieties, one form, and two hybrids in 161 genera and 66 families.

• Sijohaknonchong
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• v.21
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• pp.241-269
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• 2004

The purpose of this dissertation is to examine background of creation, composing method, and character of contents. was created by Kwon, Seop to reconfirm moral range of the Kiho-scholarship school and admire Kwon. Sang-ha (Kwon. Seop's uncle) through transmission of Kugok-ga when a custom handed down by Kwon, Sang-ha waned. This work, therefore, was composed of recollecting method describing each place of superb view relating to trace of life of Kwon, Sang-ha. In this work, the moralistic life of Kwon, Sang-ha don't appear directly. Hwangkangkugok, a fairyland where Kwon, Sang-ha lived in retirement. proves the moralistic life of Kwon, Sang-ha indirectly by describing his life as hermitian taste. Through this composing method and recognizant attitude, We know that was created for K won, Sang-ha, but it reveals Kwon, Seop' thought in life and it can be placed on prolongation with his work world.