• Korean Journal of Agricultural Science
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• v.10 no.1
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• pp.90-96
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• 1983

The short period fermentation of the mushroom compost was studied as a method for the labor saving and mechanization of composting. Rice straw was crushed, watered and supplemented with organic nutrient sources. The straw-nutrient mixtures prepared were stacked and turned for short periods at the phase I and the phase II was carried out for 8 days. The total nitrogen of the short compost was lower than that of the conventional straw compost and it caused the yield decrease. The productivity of the short compost could be improved by the supplement of nurient sources. The effective nutrients were perilla meal and rice bran. Higher microflora of the rice straw-nutrient mixture during fermentation could shorten the composting period. For the short compost method the proper period of the phase I was 5 days.

• The Korean Journal of Mycology
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• v.23 no.3 s.74
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• pp.226-231
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• 1995

Tile effects of addition of eggshell to sawdust substrate for the growth of F. velutipes were investigated. Eggshell used in this study contained 20.7% C, 0.81% N, 2530 ppm $P_2O_5$ and 44.37% Ca. The addition of eggshell resulted in the increase in bulk density and decrease of moisture content of the substrate. The addition of eggshell significantly increased the yield of the mushroom fruitbody. The addition rate of 15% (v/v), by 25% and at the rates of 5% and 10%, about 20%. Although the addition of eggshell to substrate did not improve the quality of mushroom, it increased the number of effective stipes as compared to control plot; approximately 13% more than in the control plot.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.54-54
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• 2018

억새(Miscanthus spp.)는 우리나라 등 동아시아가 원산이며 바이오매스량이 많아 바이오에너지 원료작물로서 잠재성이 크기 때문에 2세대 바이오에탄올 생산 원료로 주목을 받고 있고, 축산깔개 및 토양개량제 등으로도 이용되고 있다. 미국과 유럽 등에서는 생태계 교란 방지를 위해 4배체 물억새(M. sacchariflorus)와 2배체 참억새(M. sinensis)의 종간 교잡 이질 3배체인 불임성 억새(M. x giganteous)을 주로 재배하고 있으나, 단일 유전형의 품종으로 병해충과 자연재해에 취약하여 다양한 억새 품종의 개발이 시급한 실정이다. 본 연구는 억새를 재료로 하여 반수체 및 배수체 확보를 통한 배수성 별 특성 평가와 함께 기내배양을 통해 탈분화 및 재분화 시스템을 구축하여 억새의 육종 효율을 높이기 위해서 실시하였다. 억새 종자로부터 캘러스의 유도는 MS배지와 N6배지에 1mg/L 2.4-D를 첨가하였을 때 비배발생 캘러스(nonembryogenic callus)가 유도되었고, N6배지에 3~5 mg/L 2,4-D를 첨가하였을 때 배발생 캘러스(embryogenic callus)가 발생하였다. MS배지보다는 N6배지에서 캘러스 유도율이 높았으며, 식물생장조절제로 2,4-D와 BA 조합처리 보다 2,4-D 단용 처리하였을 때 캘러스 유도율이 더 높았다. 억새 종에 따른 캘러스 유도율은 물억새가 25.2~49.3%, 참억새는 30.3~52.0%였고 거대억새는 62.6~81.1%로 나타났다. 억새 신초 및 줄기로부터의 캘러스 유도율은 물억새가 4.4~17.2%, 참억새는 1.8~7.7%, 거대억새는 15.3~19.9%로 나타나 종자에 비해 매우 낮았다. 미성숙화기로부터의 캘러스 유도는 억새 종에 따른 차이가 없었으며, 3mg/L 2,4-D 첨가 배지에서 캘러스 유도율이 비교적 낮았고(60~80%), 1mg/L와 5mg/L의 2,4-D가 첨가된 배지에서 캘러스 유도율이 높게(90~95%) 나타났다.

• FLOWER RESEARCH JOURNAL
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• v.18 no.1
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• pp.15-22
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• 2010

These investigations were conducted to standardize several chemical and physical environments affecting in vitro propagation of gametophytes of Climacium japonicum. Propagation of this moss species was established on basal medium containing Knop macro salts and Nitsch and Nitsch trace elements. Primary cultures were initiated from apical shoots of gametophytes. Gametophyte production was accessed using chopped gametophytes, apical shoots and basal shoots. Seven ty pes of culture media and four concentrations of total nitrogen and five strengths of sucrose were tested for in vitro gametophyte production. Light and temperature factors were also evaluated. Apical shoots were the greatest among three types explants used for gametophyte propagation. Medium containing Knop macro salts and Nitsch and Nitsch trace elements was more effective than other types of media. Higher sucrose concentrations showed a positive effect on the elongation and multiplication of gametophytes. Both nitrogen deficiency and excessiveness inhibited gametophyte growth. Light intensity variation showed highly significant changes in numbers, length and fresh weight of gametophytes. Optimum light intensity for gametophyte growth seemed to be around 3000-4000 lx. Both lower and higher temperature had a negative effect on gametophyte propagation and production. This study will provide large scale and high quality propagules, and effective moss propagation system.

• Journal of Life Science
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• v.12 no.6
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• pp.740-744
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• 2002

This experiment was conducted to clarify the effects of physiological properties in culture media on the proliferation and the acclimation of bulblets of several kinds of lilies cultured in vitro. Proliferation rate of bulblets from bulblet of Oriental hybrid ‘Marco Polo’, ‘Casa Blanca’ and Asiatic hybrid ‘Jolanda’ was higher in solid MS media compared with bulblets from scale Acclimation rate of regenerated bulblets was high in solid media which was pre-cultured in vitro, compared with liquid media. Sprouting rate of bulblets from bulblet was higher than bulblets from scale.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.2
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• pp.101-105
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• 1995

This experiment was conducted to determine factors affecting callus formation of some varieties of red clover. Growth regulators. basal medium, medium pH, agar concentration, and plant age for explant were investigated. The result obtained were as follows. As auxin source, 0.1 mg/ l picloram was found to be effective on callus formation. Callus formation was the highest on PC medium among several growth media. The callus produced on PC medium was more friable than those produced on the other media. The medium pH 5.8 gave the best response for callus formation. The effective seedling age for callus formatiom was around 5-7 days old. In agar concentration, 0.5-0.8%(WN) was suitable for callus formation.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.568-572
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• 2010

In order to develop an efficient micropropagation system for a rare tree species, Empetrum nigrum var. japonicum K. Koch, the effect of medium salt, cytokinins and auxin at different concentration were evaluated. Shoot induction from axillary bud was better on WPM medium than on MS medium. Although there was no significant differences observed in shoot induction among the salt strengths of WPM medium, whereas healthy shoots were developed on basal WPM medium. In comparison of the cytokinins affecting shoot proliferation, zeatin was better than BA, whereas BA exhibited more effectiveness on shoot elongation. In vitro root formation was better on WPM medium than on 1/2MS medium and achieved the highest rooting rate when 5.0 mg/L IBA treatment. 93% of rooted plantlets were survived on artificial soil mixture after 4 weeks of acclimatization. Above results suggest that a rare tree species, E. nigrum var. japonicum can be micropropagated via axillary bud cultures.

• Journal of Practical Agriculture & Fisheries Research
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• v.14 no.1
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• pp.195-209
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• 2012

1) 버섯종균 생산 시스템 개선 버섯의 품질과 수량을 좌우하는 주요 요인은 종균의 활력이다. 그러나 말라위에서는 종균의 활력이 낮아 조그마한 350cc 음료수 병에 배양 완성하는데 20일이 소요되는 등 노동력의 비효율성을 개선하였다. 따라서 350cc 병을 3500cc 비닐봉지로 개선하여 입봉하는데 소요되는 시간을 10배 이상으로 단축 개선하였다. 또한 곡립종균을 액체종균으로 대처한 결과 균사생장 속도가 빨랐으며 작업속도가 빨라졌다. 곡립 수수를 밀로 대체하여 종균제조 노동력을 효율적으로 전환하였다. 2) 버섯재배사 환경관리 개선 버섯재배의 4대 환경조건은 온도, 습도, 환기, 광이다. 그러나 말라위의 버섯재배사는 4대 환경조건 조절을 자연상태에 의존하고 있다. 그럼에도 불구하고 비닐로 커버된 상태에서 문을 닫아두어 과습이 되고 환기가 되지않았다. 이를 개선하기 위하여 직경 5cm 환기공을 버섯재배사 당 4개씩 만들도록 지도하였다. 3) 버섯배지 개선 말라위에서는 옥수수 대를 잘라 버섯배지로 주로 이용하고 있다. 이를 개선하여 말라위 북부지역(다광)에 대규모 사탕수수 공장을 방문하여 사탕수수 박을 이용하여 버섯재배 기술을 교육하였다. 이를 이용함으로서 버섯 균사생장속도를 빠르게 하고 균사량의 축적을 높이도록 하여 고품질 다수확하는데 교육을 하고 컨설팅을 실시하여 개선하였다. 4) 버섯 배지 야외발효 후 지면재배 시도로 시간 단축과 비용절감 말라위 버섯배지 혼합제조법은 옥수수대 한가지로서 영양원이 없다. 이를 개선하여 배지를 수분을 흡수시킨 후 야외 퇴적하므로서 배지의 물리성을 개선하고 야외발효 과정 중 양분이 합성되어 양질의 배지를 만드는 방법이다. 야외발효한 배지를 봉지에 담아 균상에 입상하는 불편함과 나무로 만들어진 균상대의 과습으로 인한 곰팡이 발생과 개미 침입으로 썩는 것 등을 방지하기 위하여 지면재배 방법으로 개선하였다. 이 지면재배는 균상 만드는 나무재료와 인건비 절감. 병해충 발생 방제 뿐만아니라 고품질 다수확할 수 있는 방법으로 개선 교육과 시범 시연을 실시하였다.

• Journal of Mushroom
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• v.13 no.3
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• pp.233-236
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• 2015

This report is the result of devising a method for utilizing the device of the load cell to maintain a constant water content of the medium every day to prepare a cultural substrates with the mixer for growing mushrooms bottle cultivation. A load cell was device under the medium mixer. It is developed when the device reaches the weight calculated as amount of substrate bottled and number of the bottle, it is automatically terminated by water injection. In addition, measuring the water content of each medium and the total weight of the medium reaches the target moisture content were calculated by using the program Cheong et al. (2015). Enter the total weight of the medium on the display unit of the load cell, when starting the water supply to reach the weight-based mixing media, the water supply is stopped. This method can improve the convenience by reducing the user's trouble in repeated work medium prepared by automating water supply. The suitable moisture content of the mixed medium for some kind of mushroom can be improved by the composition accuracy. And mycelial culture period, primordial period, mushroom growing period is maintained even of the medium can be produced stably. Therefore, it is possible to achieve a stable management of the mushroom farm according to mushroom quality and quantity stable throughout the year.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2018.11a
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• pp.130-132
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• 2018

등부표는 항로를 가리키는 표지의 한 가지로써 암초나 얕은 곳 따위를 알리는 중요한 역할을 한다. 본 연구에서는 LED 등부표의 경량화, 내부식성, 높은 기계적 물성확보를 위해 탄소섬유/에폭시 복합재료를 적용하였다. 더불어, 자가발전이 가능한 스마트 LED 등부표 개발을 위해 전기활성고분자 필름을 적용하여 가능성을 확인하였다.